万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
深圳子科生物科技有限公司
- 英文名:
LactobacilliAgar,AOAC
中文名称:乳酸杆菌琼脂,AOAC
规格:100g/瓶
品牌:印度HiMeida
货号:M366-100g 英文:LactobacilliAgar,AOAC
详细说明书,报价,货期可以咨询在线客服或+wv 13924667705沟通了解。
Lactobacilli Agar, AOAC is used for carrying the stock cultures used in microbiological assays of the B-vitamins.
Composition**
| Ingredients | Gms / Litre |
|---|---|
| Peptonized milk | 15.000 |
| Yeast extract | 5.000 |
| Dextrose | 10.000 |
| Tomato juice (100 ml) | 5.000 |
| Monopotassium phosphate | 2.000 |
| Polysorbate 80 | 1.000 |
| Agar | 10.000 |
Final pH (at 25°C): 6.8±0.2
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 48 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Distribute into tubes in 10 ml amounts and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool the tubed agar medium in an upright position.
Principle And Interpretation
Lactobacilli Agar, AOAC was formulated by Loy (1) and recommended by AOAC (2) for carrying stock cultures used for Microbiological assay of Vitamin B. Lactobacilli Agar, AOAC is used for the cultivation and maintenance of stock cultures of Lactobacillus casei ATCC 7469, Lactobacillus fermentum ATCC 9338, Lactobacillus leichmannii ATCC 4797, and Lactobacillus viridescens ATCC 12706 by stab inoculation of sterile tubed medium followed by incubation for 18-24 hours at a constant temperature between 30-40°C. The cultures are then stored at 2-8°C.
Peptonized milk and yeast extract provide essential growth nutrients. Dextrose is the energy source. Phosphate provides buffering system while tomato juice helps in lowering the pH. Polysorbate 80 serves as an emulsifier.
Before using a culture in any assay, at least 2 successive transfers during a 1-2 week period are essential. Any culture older than one week should not be used.
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验样品中变性程度较深的那部分。新西兰Fonterra科学家Don Otter以及我们实验室的研究均证实,酪蛋白脱除对于该法获得准确检测结果具有重要意义。此外,Don Otter课题组已经正式向AOAC提交了相关论文。 色谱柱:牛初乳或免疫奶粉样品中的IgG可以采用亲合HPLC检测(Pharmacia Hi-Trap 蛋白质 G 柱),亲和介质为结合Protein G的琼脂糖(Sepharose 4B F.F.)。上述亲合材料已经通过基因工程手段剪切了Protein G中结合白蛋白的部位
一、目的要求 1. 学习分离、纯化噬菌体的基本原理和方法。 2. 观察噬菌斑。 二、基本原理 因为噬菌体是专性寄生物,所以自然界中凡有细菌分布的地方,均可发现其特异的噬菌体的存在,亦即噬菌体是伴随着宿主细菌的分布而分布的,例如粪便与阴沟污水中含有大量大肠杆菌,故也能很容易地分离到大肠杆菌噬菌体;乳牛场有较多的乳酸杆菌,也容易分离到乳酸杆菌噬菌体等。 由于噬菌体侵入细菌细胞后进行复制而导致细胞裂解,噬菌体即从中释放出来,所以 (a)在液体培养基内可使
:双歧杆菌、乳酸杆菌、乳酸球菌由本实验室筛选,经省级权威部门鉴定符合生产和临床使用标准。 2、培养基: 2.1、改良TJA培养基(培养双歧杆菌用):番茄汁50mL;酵母浸出物5g;肉膏lOg;乳糖20g;葡萄糖2g;K 2 HPO 4 2g;Tween80 1g;醋酸钠2.5g;琼脂15
技术资料暂无技术资料 索取技术资料










