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文献和实验Interaction Trap/Two‐Hybrid System to Identify Loss‐of‐Interaction Mutant Proteins
to a specific partner protein is described. The method takes advantage of the ease of the interaction trap/two?hybrid system and PCR mutagenesis. PCR fusion of the target protein to GFP is used to ensure the protein's open reading frame is not disrupted
of 7 x 2 x 0.5" and bottom dimensions of 7 x 2 x 0.25". 2) Recess the top plate in the center portion to 0.125" deep, leaving 0.950" per end for 1.375" wide x 0.3125" deep fluid reservoir. 3) Drill 1/16" wide x 0.625" long
【精华】vol 687 Chapter 3 采用Splinkerette-PCR技术对前病毒基因组插入位点进行分离
of methods used to uncover insertion sites have previouslybeen described, including genomic DNA library screening(15), ligation-mediated PCR (6), inverse PCR (16), VISAtechnique (17), T-linker PCR (18), and single nucleotidepolymorphism-based mapping (19
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