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文献和实验Materials2 ml size Sarstedt screw cap tubesBIO-RAD Disposable Chromatography Column （BIO-RAD cat. #732-6008）Oligo dT celluloseRNase-free water5.0 M NaCl0.5 M EDTA1.0 M Tris, pH 7.410% SDS70% EtOHBefore starting protocol1. Measure out 75 mg of oligo
also can be concentrated by polymerization induced by addition of 1/9 volume of 10X MKEI buffer (1 M KCl, 20 mM MgCl2, 10 mM EGTA and 0.2 M imidazole-HCl, pH 7.0) and incubated overnight at room temperature. Actin was pelleted by centrifugation at 132,00 x g in a Ti75
x Column Buffer ~10 L 1x column buffer 10 M NaOH 3 fresh ( Equipment: Waring Blender Temperature controlled ultracentrifuge (Beckman L7-55) with the equivalent of a Beckman 50.2Ti rotor At least 24 31.5 ml thick-walled polycarbonate ultracentrifuge tubes
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