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文献和实验with occasional vortexing.Wash the gel pieces with ~100 ml 25 mM NH4HCO3 for 10 min while vortexing,dehydrate with ~100 ml 25 mM NH4HCO3 in 50% acetonitrile and rehydrate again with ~100 ml 25 mM NH4HCO3 and dehydrate again.Remove the liquid phase and dry the gel
In-gel digestion of proteins for peptide fingerprint mapping
Dissolve trypsin just before use in ice-cold buffer (to reduce auto-proteolysis). 1. Excise protein bands/spots of interest from the gel and cut each gel piece into small particles (~1 mm x 1 mm) using a scalpel and place into a 0.65 ml
acetonitrile as needed for solubility. Filter clear solution of crude peptoid product with 0.45 μm syringe filter to remove dust and particles. 3) Analytical HPLC and electrospray LC-MS: Prepare a ˜ 20 μg/mL crude peptoid solution. Filter
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