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文献和实验Purification of Plasmid from 50 ml-culture
4. Resuspend the pellet in 5 ml of Solution I. 5. Add 10 ml of Solution II. Mix well by inverting the bottle more than 10 times. The solution should be clear after mixing Solution II. 6. Add 7.5 ml of Solution III. Mix well as above. 7. Spin
Mag-Bind Blood DNA Maximum Yield Protocol (2ml-6ml)
实验试剂 1. Absolute ethanol (96%-100%) 实验设备 1. Nuclease-free 50 ml centrifuge tube 2. Water bath, incubator or heating block preset at 65°C 3. Magnetic separation device for 50 ml tube
BAC DNA Isolation from 200 ml Cultures by a Cleared Lysate Method
7.6, 50 mM EDTA, pH 8.0), and 1.8 ml of 7.5 M KOAc (note: the 7.5 M KOAc solution is used without pH adjustment) is added into each bottle and transfered to a 50 ml Sorval centrifuge tube. 8、After mixing, the tubes were frozen, until solid, at -70
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