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文献和实验祝贺〖雅裕睿安〗成为Signalway Antibody(SAB)独家
antibody unconjugated; 货号21337,产品描述:GAPDH Antibody;规格 50 ul / 100 ul; 货号3052,产品描述:Rabbit anti-Human IgG secondary antibody HRP conjugated; 货号300904050,产品描述:One-Hour Western kit,10 ml / bottle x 3;
v468. chapter 4 WNT 细胞通路 检测细胞GSK3活性与表达水平
) (see Note 5 ). 5. Rabbit anti-mouse (#31450), sheep anti-rabbit (#31460)and donkey anti-sheep (#31480) horseradish peroxidaseconjugatedsecondary antibodies are available from Pierce(Rockford, IL). 6. Enhanced chemiluminescence (ECL) reagent (#RPN2106
In Situ Hybridization Protocols
. Incubate at 70deg.C for 30 minutes. Vortex hard for 10 minutes. Cool mixture on ice, add 5ul sheep serum and 1ul anti-digoxygenin antibody. Mix for 1 hour at 4deg.C. Spin down hard and dilute supernatant to 2ml with PBT containing
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