- 询价
- Invitrogen
- 美国
- CPC0043
- 2026年03月14日
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文献和实验Human IL-2 Enzyme Immunoassay (EIA)
EIA is an in vitro enzyme-linked immunosorbent assay for quantitatively detecting human IL-2 in biological fluids (e.g. serum, plasma) and in cell culture supernatants. The kit consists of a 96 well microtiter plate that has been precoated with anti-IL-2
obtaining good signals when we've used poor quality BSA. Others have told me that 10% nonfat dry milk works well for anti-PCREB, but in my hands milk is not as good as BSA from USB (it seems to mask the epitope).9. Remove block solution and add primary
Dual-Color ELISPOT Assay for the Simultaneous Detection of IL-2 and/or IFN- Secreting T Cells
. 3. Add 100 µL of coating buffer (dual, IL-2, and IFN- ) to the appropriate wells. 4. Seal plate in plastic wrap and incubate overnight at 4°C. Thawing Cells (30 min) 5. Remove frozen PBMCs from the liquid
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