pLV2-TRE3GS-3×HA-mherry-RETREG

pLV2-TRE3GS-3×HA-mherry-RETREG1-TetOne-Puro plasmid

PVT112018

pLV2-TRE3GS-3×HA-mherry-RETREG1-TetOne-Puro plasmid Information

Plasmid name: pLV2-TRE3GS-3×HA-mherry-RETREG1(human)-TetOne-Puro
Alias: NM_001034850.3;FAM134B; JK-1; JK1
Gene lenght: 1494
Host: Mammalian cells,lentivirus
Use(s): Gene expression,Tetracycline induction
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: TRE3GS
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: mCherry-F
Reverse primer:
Copy number: High
Induction: Tetracycline induction

pLV2-TRE3GS-3×HA-mherry-RETREG1-TetOne-Puro plasmid Description

pLV2-TRE3GS-3×HA-mherry-RETREG1-TetOne-Puro is a plasmid suitable for gene expression in mammalian cells, tetracycline-inducible, and can also be used for lentivirus packaging.

pLV2-TRE3GS-3×HA-mherry-RETREG1-TetOne-Puro plasmid Sequence

1 actactaagt ttgtagtaca tatttaacaa atacaatttc tttaaaaact actaagtttg 61 tagtacatat ttaacaaata caatttcttt aaaatgaaaa taattcagag gaatcacagg 121 tttagagtaa atgaaaccac taggtaattg gcagtggtaa tagggtatgg ggtgggaagt 181 ttgggatgat tttggttagc ttgagttatc cagttgatcc agacatgata agatacattg 241 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt 301 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt gatctcccga 361 tccgtcgacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt 421 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca 481 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt 541 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga 601 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa 661 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct 721 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat 781 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga 841 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc 901 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat 961 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa 1021 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac 1081 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa 1141 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc 1201 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc 1261 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag 1321 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta 1381 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa 1441 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc 1501 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat 1561 ctgctgcttg caaacaaaaa aaccgctacc agcggtggtt tgtttgccgg atcaagagct 1621 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct 1681 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct 1741 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg 1801 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc 1861 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga 1921 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg 1981 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta 2041 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg 2101 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg 2161 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat 2221 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc 2281 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc 2341 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa 2401 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc 2461 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga 2521 ccatgattac gccaagctct agctagaggt cgacggtaaa acgctagcaa tgtcaaggcc 2581 tctcactctc tgatattcat ttctttgcaa gttataaata ctgaataata agatgacatg 2641 aactactact gctagagatt ttccacactg actaaaaggg tctgagggat ctctagttac 2701 cagagtcaca caacagacgg gcacacacta cttgaagcac tcaaggcaag ctttattgag 2761 gcttaagcag tgggttccct agttagccag agagctccca ggctcagatc tggtctaacc 2821 agagagaccc agtacaggca aaaagcagct gcttatatgc aggatctgag ggctcgccac 2881 tccccagtcc cgcccaggcc acgcctccct ggaaagtccc cagcggaaag tcccttgtag 2941 caagctcgat atcagcagtt cttgaagtac tccggatgca gctctcgggc cacgtgatga 3001 aatgctaggc ggctgtcaaa cctccactct aacacttctc tctccgggtc atccatccca 3061 tgcaggctca cagggtgtaa caagctggtg ttctctcctt tattggcctc ttctacctta 3121 tctggctcaa ctggtactag cttgtagcac catccaaagg tcagtggata tctgacccct 3181 ggccctggtg tgtagttctg ctaatcaggg aagtagcctt gtgtgtggta gatccacaga 3241 tcaaggatat cttgtcttcg ttgggagtga attagccctt ccagtcccct cttttctttt 3301 aaaaagtggc taagatctac agctgccttg taagtcattg gtcttaaagg tacctgaggt 3361 gtgactggaa aacccacctc ctcctcctct tgtgcttcta gccaggcaca atcagcattg 3421 gtagctgctg tattgctact tgtgattgct ccatgttttt ctaggtctcg actgcagaat 3481 taattccagg cggggaggcg gcccaaaggg agatccgact cgtctgaggg cgaaggcgaa 3541 gacgcggaag aggccgcaga gccggcagca ggccgcggga aggaaggtcc gctggattga 3601 gggccgaagg gacgtagcag aaggacgtcc cgcgcagaat ccaggtggca acacaggcga 3661 gcagccatgg aaaggacgtc agcttccccg acaacaccac ggaattgtca gtgcccaaca 3721 gccgagcccc tgtccagcag cgggcaaggc aggcggcgat gagttccgcc gtggcaatag 3781 ggagggggaa agcgaaagtc ccggaaagga gctgacaggt ggtggcaatg ccccaaccag 3841 tgggggttgc gtcagcaaac acagtgcaca ccacgccacg ttgcctgaca acgggccaca 3901 actcctcata aagagacagc aaccaggatt tatacaagga ggagaaaatg aaagccatac 3961 gggaagcaat agcatgatac aaaggcatta aagcagcgta tccacatagc gtaaaaggag 4021 caacatagtt aagaatacca gtcaatcttt cacaaatttt gtaatccaga ggttgattgt 4081 tccagacgcg ttcaggcacc gggcttgcgg gtcatgcacc aggtgcgcgg tccttcgggc 4141 acctcgacgt cggcggtgac ggtgaagccg agccgctcgt agaaggggag gttgcggggc 4201 gcggaggtct ccaggaaggc gggcaccccg gcgcgctcgg ccgcctccac tccggggagc 4261 acgacggcgc tgcccagacc cttgccctgg tggtcgggcg agacgccgac ggtggccagg 4321 aaccacgcgg gctccttggg ccggtgcggc gccaggaggc cttccatctg ttgctgcgcg 4381 gccagccggg aaccgctcaa ctcggccatg cgcgggccga tctcggcgaa caccgccccc 4441 gcttcgacgc tctccggcgt ggtccagacc gccaccgcgg cgccgtcgtc cgcgacccac 4501 accttgccga tgtcgagccc gacgcgcgtg aggaagagtt cttgcagctc ggtgacccgc 4561 tcgatgtggc ggtccgggtc gacggtgtgg cgcgtggcgg ggtagtcggc gaacgcggcg 4621 gcgagggtgc gtacggcccg ggggacgtcg tcgcgggtgg cgaggcgcac cgtgggcttg 4681 tactcggtca tggtcgcgtt ttgcaaaagc ctaggcctcc aaaaaagcct cctcactact 4741 tctggaatag ctcagaggcc gaggcggcct cggcctctgc ataaataaaa aaaattagtc 4801 agccatgggg cggagaatgg gcggaactgg gcggagttag gggcgggatg ggcggagtta 4861 ggggcgggac tatggttgct gactaattga gatgcatgct ttgcatactt ctgcctgctg 4921 gggagcctgg ggactttcca cacctggttg ctgactaatt gagatgcatg ctttgcatac 4981 ttctgcctgc tggggagcct ggggactttc cacaccctaa ctgacacaca ttcgcgcgtt 5041 tacccgggga gcatgtcaag gtcaaaatcg tcaagagcgt cagcaggcag catatcaagg 5101 tcaaagtcgt caagggcatc ggctgggagc atgtctaagt caaaatcgtc aagggcgtcg 5161 gtcggcccgc cgctttcgca ctttagctgt ttctccaggc cacatatgat tagttccagg 5221 ccgaaaagga aggcaggttc ggctccctgc cggtcgaaca gctcaattgc ttgtttcaga 5281 agtgggggca tagaatcggt ggtaggtgtc tctctttcct cttttgctac ttgatgctcc 5341 tgttcctcca atacgcagcc cagtgtaaag tggcccacgg cggacagagc gtacagtgcg 5401 ttctccaggg agaagccttg ctgacacagg aacgcgagct gattttccag ggtttcgtac 5461 tgtttctctg ttgggcgggt gccgagatgc actttagccc cgtcgcgatg tgagaggaga 5521 gcacagcggt atgacttggc gttgttccgc agaaagtctt gccatgactc gccttccagg 5581 gggcaggagt gggtatgatg cctgtccagc atctcgattg gcagggcatc gagcagggcc 5641 cgcttgttct tcacgtgcca gtacagggta ggctgctcaa ctcccagctt ttgagcgagt 5701 ttccttgtcg tcaggccttc gataccgact ccattgagta attccagagc agagtttatg 5761 actttgctct tgtccagtct agacatggta attcgatgat ccccctgggg agagaggtcg 5821 gtgattcggt caacgaggga gccgactgcc gacgtgcgct ccggaggctt gcagaatgcg 5881 gaacaccgcg cgggcaggaa cagggcccac actaccgccc cacaccccgc ctcccgcacc 5941 gccccttccc ggccgctgct ctcggcgcgc cctgctgagc agccgctatt ggccacagcc 6001 catcgcggtc ggcgcgctgc cattgctccc tggcgctgtc cgtctgcgag ggtactagtg 6061 agacgtgcgg cttccgtttg tcacgtccgg cacgccgcga accgcaagga accttcccga 6121 cttaggggcg gagcaggaag cgtcgccggg gggcccacaa gggtagcggc gaagatccgg 6181 gtgacgctgc gaacggacgt gaagaatgtg cgagacccag ggtcggcgcc gctgcgtttc 6241 ccggaaccac gcccagagca gccgcgtccc tgcgcaaacc cagggctgcc ttggaaaagg 6301 cgcaacccca accccgtgga attatcacct cgagtttact ccctatcagt gatagagaac 6361 gtatgaagag tttactccct atcagtgata gagaacgtat gcagacttta ctccctatca 6421 gtgatagaga acgtataagg agtttactcc ctatcagtga tagagaacgt atgaccagtt 6481 tactccctat cagtgataga gaacgtatct acagtttact ccctatcagt gatagagaac 6541 gtatatccag tttactccct atcagtgata gagaacgtat aagctttgct tatgtaaacc 6601 agggcgccta taaaagagtg ctgatttttt gagtaaactt caattccaca acacttttgt 6661 cttataccaa ctttccgtac cacttcctac cctcgtaaag aattcgccac catgTACCCA 6721 TACGACGTAC CTGATTATGC AACTTATCCT TATGATGTAC CTGATTATGC TAGCTATCCT 6781 TATGATGTCC CAGACTACGC Aggggatcca ccggtcgcca ccatggtgag caagggcgag 6841 gaggataaca tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc 6901 gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc 6961 cagaccgcca agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg 7021 tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc accccgccga catccccgac 7081 tacttgaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac 7141 ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggcgagtt catctacaag 7201 gtgaagctgc gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg 7261 ggctgggagg cctcctccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc 7321 aagcagaggc tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa gaccacctac 7381 aaggccaaga agcccgtgca gctgcccggc gcctacaacg tcaacatcaa gttggacatc 7441 acctcccaca acgaggacta caccatcgtg gaacagtacg aacgcgccga gggccgccac 7501 tccaccggcg gcatggacga gctgtacaag gGCGGCGGAG GGAGTGGAGG AGGGGGAAGC 7561 ctcgagatgg cgagcccggc gcctccggag cacgccgagg agggatgccc ggctcctgcc 7621 gccgaggagc aggcgccgcc gtcgccgcca ccgccccagg catcccccgc agagcggcag 7681 cagcaggagg aggaagcgca ggaagctggg gcggcggagg gcgcggggtt gcaggtggag 7741 gaggccgcgg gccgggcggc ggccgcggta acctggctgc tcggggagcc ggtgctgtgg 7801 ctgggctgcc gcgccgacga gctgctgagc tggaagaggc cgctgcggag cctgctcggc 7861 ttcgtcgctg ccaacctgct gttctggttc cttgcattga ctccatggag agtatatcac 7921 ctgatttccg tcatgatact tgggcgtgtt attatgcaaa taataaagga tatggttttg 7981 tctagaacaa gaggtgcaca gttgtggaga agcctcagtg aaagctggga agttatcaat 8041 tccaaaccag atgaaagacc caggctcagc cactgtattg cagaatcatg gatgaatttc 8101 agcatatttc ttcaagaaat gtctcttttt aaacagcaga gccctggcaa gttttgtctc 8161 ctggtctgta gtgtgtgcac attttttacg atcttgggaa gttacattcc tggggttata 8221 ctcagctatc tactgttact gtgtgcattt ttgtgtccat tgtttaaatg taatgatatt 8281 ggacaaaaaa tttacagcaa aattaagtca gttctgctga aactggattt tggaattgga 8341 gaatatatta atcagaagaa acgtgagaga tctgaagcag acaaagaaaa aagtcacaaa 8401 gatgacagtg aattagactt ttcagctctt tgtcctaaga ttagcctcac ggttgctgcc 8461 aaagagttat ctgtgtctga cacagacgtc tcagaggtat cctggactga taatgggacc 8521 ttcaaccttt cagaaggata cactccacag acagacactt ctgatgatct tgaccgaccc 8581 agtgaggaag ttttctctag agatctttca gattttccat ctctagaaaa tggcatggga 8641 acaaatgatg aagatgaatt aagccttggt ttgcccactg agctcaagag aaagaaggaa 8701 cagttggaca gtggtcacag accaagcaaa gagacgcaat cagcagctgg tctcaccctt 8761 cctctgaaca gtgaccaaac ctttcacctg atgagcaacc tggctgggga tgttatcaca 8821 gctgcagtga ctgcagctat caaagaccag ttagagggtg tgcagcaagc actttctcag 8881 gctgccccca tcccagaaga ggacacagac actgaagaag gtgatgactt tgaactactt 8941 gaccagtcag agctggatca aattgagagt gaattgggac ttacacaaga ccaggaagca 9001 gaagcacagc aaaataagaa gtcttcaggt ttcctttcaa atctgctggg aggccattaa 9061 ggatccagac cacctcccct gcgagctaag ctggacagcc aatgacgggt aagagagtga 9121 catttttcac taacctaaga caggagggcc gtcagagcta ctgcctaatc caaagacggg 9181 taaaagtgat aaaaatgtat cactccaacc taagacaggc gcagcttccg agggatttga 9241 gatccagaca tgataagata cattgatgag tttggacaaa ccaaaactag aatgcagtga 9301 aaaaaatgcc ttatttgtga aatttgtgat gctattgcct tatttgtaac cattataagc 9361 tgcaataaac aagttaagtc gataaactgg atctctgctg tccctgtaat aaacccgaaa 9421 attttgaatt tttgtaattt gtttttgtag ttctttagtt tgtatgtctg ttgctattat 9481 gtctactatt ctttcccctg cactgtaccc cccaatcccc ccttttcttt taaaggcgat 9541 accgtcgaga tccgttcact aatcgaatgg atctgtctct gtctctctct ccaccttctt 9601 cttctattcc ttcgggcctg tcgggtcccc tcggggttgg gaggtgggtc tgaaacgata 9661 atggtgaata tccctgccta actctattca ctatagaaag tacagcaaaa actattctta 9721 aacctaccaa gcctcctact atcattatga ataattttat ataccacagc caatttgtta 9781 tgttaaacca attccacaaa cttgcccatt tatctaattc caataattct tgttcattct 9841 tttcttgctg gttttgcgat tcttcaatta aggagtgtat taagcttgtg taattgttaa 9901 tttctctgtc ccactccatc caggtcgtgt gattccaaat ctgttccaga gatttattac 9961 tccaactagc attccaaggc acagcagtgg tgcaaatgag ttttccagag caaccccaaa 10021 tccccaggag ctgttgatcc tttaggtatc tttccacagc caggattctt gcctggagct 10081 gcttgatgcc ccagactgtg agttgcaaca gatgctgttg cgcctcaata gccctcagca 10141 aattgttctg ctgctgcact ataccagaca ataattgtct ggcctgtacc gtcagcgtca 10201 ttgacgctgc gcccatagtg cttcctgctg ctcccaagaa cccaaggaac aaagctccta 10261 ttcccactgc tcttttttct ctctgcacca ctcttctctt tgccttggtg ggtgctactc 10321 ctaatggttc aatttttact actttatatt tatataattc acttctccaa ttgtccctca 10381 tatctcctcc tccaggtctg aagatcagcg gccggccgct tgctgtgcgg tggtcttact 10441 tttgttttgc tcttcctcta tcttgtctaa agcttccttg gtgtctttta tctctatcct 10501 ttgatgcaca caatagaggg ttgctactgt attatataat gatctaagtt cttctgatcc 10561 tgtctgaagg gatggttgta gctgtcccag tatttgtcta cagccttctg atgtttctaa 10621 caggccagga ttaactgcga atcgttctag ctccctgctt gcccatacta tatgttttaa 10681 tttatatttt ttctttcccc ctggccttaa ccgaattttt tcccatcgcg atctaattct 10741 cccccgctta atactgacgc tctcgcaccc atctctctcc ttctagcctc cgctagtcaa 10801 aatttttggc gtactcacca gtcgccgccc ctcgcctctt gccgtgcgcg cttcagcaag 10861 ccgagtcctg cgtcgagaga gctcctctgg tttccctttc gctttcaagt ccctgttcgg 10921 gcgccactgc tagagatttt ccacactgac taaaagggtc tgagggatct ctagttacca 10981 gagtcacaca acagacgggc acacactact tgaagcactc aaggcaagct ttattgaggc 11041 ttaagcagtg ggttccctag ttagccagag agctcccagg ctcagatctg gtctaaccag 11101 agagacccag tacaggcaaa aagcagctgc ttatatgcag gatctgaggg ctcgccactc 11161 cccagtcccg cccaggccac gcctccctgg aaagtcccca gcggaaagtc ccttgtagca 11221 agctcgatat cagcagttct tgaagtactc cggatgcagc tctcgggcca cgtgatgaaa 11281 tgctaggcgg ctgtcaaacc tccactctaa cacttctctc tccgggtcat ccatcccatg 11341 caggctcaca gggtgtaaca agctggtgtt ctctccttta ttggcctctt ctaccttatc 11401 tggctcaact ggtactagct tgtagcacca tccaaaggtc agtggatatc tgacccctgg 11461 ccctggtgtg tagttctgct aatcagggaa gtagccttgt gtgtggtaga tccacagatc 11521 aaggatatct tgtcttcttt gggagtgaat tagcccttcc a

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.