pAAV-CMV-mCherry-U6-Col6a3-shR

pAAV-CMV-mCherry-U6-Col6a3-shRNA1 plasmid

PVT111802

pAAV-CMV-mCherry-U6-Col6a3-shRNA1 plasmid Information

Plasmid name: pAAV-CMV-mCherry-U6-Col6a3(mouse)-shRNA1
Alias: AGTTCTATTTAAATTCCTA
Gene lenght:
Host: Mammalian cells,Adeno associated virus
Use(s): Gene interference
Fragment type: shRNA
Species: Mouse
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling: Red
Promoter: U6
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: MCHERRY-F
Reverse primer:
Copy number: High
Induction:

pAAV-CMV-mCherry-U6-Col6a3-shRNA1 plasmid Description

pAAV-CMV-mCherry-U6-Col6a3-shRNA1 This plasmid is suitable for gene interference in mammalian cells and can also be used for adeno-associated virus packaging.

pAAV-CMV-mCherry-U6-Col6a3-shRNA1 plasmid Sequence

1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca 121 actccatcac taggggttcc tgcggccgca cgcgtggagc tagttattaa tagtaatcaa 181 ttacggggtc attagttcat agcccatata tggagttccg CGTTACATAA CTTACGGTAA 241 ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT GACGTCAATA ATGACGTATG 301 TTCCCATAGT AACGcCAATA GGGACTTTCC ATTGACGTCA ATGGGTGGAG TATTTACGGT 361 AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC AAGTACGCCC CCTATTGACG 421 TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA CATGACCTTA TGGGACTTTC 481 CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC CATGGTGATG CGGTTTTGGC 541 AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG ATTTCCAAGT CTCCACCCCA 601 TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG GGACTTTCCA AAATGTCGTA 661 ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA 721 GCAGAGCTCG TTTAGTGAAC CGTCAGATCG CCTGGAGACG CCATCCACGC TGTTTTGACC 781 TCCATAGAAG ACACCGGGAC CGATCCAGCC TCCGCGGATT CGAATCCCGG CCGGGAACGG 841 TGCATTGGAA CGCGGATTCC CCGTGCCAAG AGTGACGTAA GTACCGCCTA TAGAGTCTAT 901 AGGCCCACAA AAAATGCTTT CTTCTTTTAA TATACTTTTT TGTTTATCTT ATTTCTAATA 961 CTTTCCCTAA TCTCTTTCTT TCAGGGCAAT AATGATACAA TGTATCATGC CTCTTTGCAC 1021 CATTCTAAAG AATAACAGTG ATAATTTCTG GGTTAAGGCA ATAGCAATAT TTCTGCATAT 1081 AAATATTTCT GCATATAAAT TGTAACTGAT GTAAGAGGTT TCATATTGCT AATAGCAGCT 1141 ACAATCCAGC TACCATTCTG CTTTTATTTT ATGGTTGGGA TAAGGCTGGA TTATTCTGAG 1201 TCCAAGCTAG GCCCTTTTGC TAATCATGTT CATACCTCTT ATCTTCCTCC CACAGCTCCT 1261 GGGCAACGTG CTGGTCTGTG TGCTGGCCCA TCACTTTGGC AAAGAATTGG GATTCGAACA 1321 TCGATTGAAT TCGCTAGCGT TTAAACTTAA GCTTGGTACC ACTAGTGATA TCTCTAGAGG 1381 GCCCAGATCT ACgggtggca tccctgtgac ccctccccag tgcctctcct ggccctggaa 1441 gttgccactc cagtgcccac cagccttgtc ctaataaaat taagttgcat cattttgtct 1501 gactaggtgt ccttctataa tattatgggg tggagggggg tggtatggag caaggggcaa 1561 gttgggaaga caacctgtag ggcctgcggg gtctattggg aaccaagctg gagtgcagtg 1621 gcacaatctt ggctcactgc aatctccgcc tcctgggttc aagcgattct cctgcctcag 1681 cctcccgagt tgttgggatt ccaggcatgc atgaccaggc tcagctaatt tttgtttttt 1741 tggtagagac ggggtttcac catattggcc aggctggtct ccaactccta atctcaggtg 1801 atctacccac cttggcctcc caaattgctg ggattacagg cgtgaaccac tgctcccttc 1861 cctgtccttc tgattttgta ggtaaccacg tgcggaccTC GAAATTCCCC AGTGGAAAGA 1921 CGCGCAGGCA AAACGCACCA CGTGACGGAG CGTGACCGCG CGCCGAGCGC GCGCCAAGGT 1981 CGGGCAGGAA GAGGGCCTAT TTCCCATGAT TCCTTCATAT TTGCATATAC GATACAAGGC 2041 TGTTAGAGAG ATAATTAGAA TTAATTTGAC TGTAAACACA AAGATATTAG TACAAAATAC 2101 GTGACGTAGA AAGTAATAAT TTCTTGGGTA GTTTGCAGTT TTAAAATTAT GTTTTAAAAT 2161 GGACTATCAT ATGCTTACCG TAACTTGAAA GTATTTCGAT TTCTTGGGTT TATATATCTT 2221 GTGGAAAGGA CGCGGGATCC GAGTTCTATT TAAATTCCTA ttcaagagaT AGGAATTTAA 2281 ATAGAACTtt ttttCTCGAG TTAGAGTCCT GCAGCTCGCT TTTTAAGCTT GCGGccgcag 2341 gaacccctag tgatggagtt ggccactccc tctctgcgcg ctcgctcgct cactgaggcc 2401 gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg cggcctcagt gagcgagcga 2461 gcgcgcagct gcctgcaggg gcgcctgatg cggtattttc tccttacgca tctgtgcggt 2521 atttcacacc gcatacgtca aagcaaccat agtacgcgcc ctgtagcggc gcattaagcg 2581 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg 2641 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc 2701 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa 2761 aacttgattt gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc 2821 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac 2881 tcaaccctat ctcgggctat tcttttgatt tataagggat tttgccgatt tcggcctatt 2941 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt 3001 ttacaatttt atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc 3061 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg 3121 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat 3181 caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca 3241 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc 3301 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct 3361 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg 3421 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg 3481 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc 3541 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca 3601 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac 3661 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa 3721 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg 3781 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt 3841 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg 3901 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc 3961 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga 4021 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta 4081 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc 4141 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg 4201 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt 4261 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa 4321 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt 4381 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt 4441 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt 4501 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga 4561 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag 4621 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata 4681 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg 4741 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga 4801 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca 4861 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa 4921 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt 4981 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac 5041 ggttcctggc cttttgctgg ccttttgctc acatgt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.