pCMV-Cxcr3-3×HA-mCMV-EGFP-Neo

pCMV-Cxcr3-3×HA-mCMV-EGFP-Neo plasmid

PVT111511

pCMV-Cxcr3-3×HA-mCMV-EGFP-Neo plasmid Information

Plasmid name: pCMV-Cxcr3(mouse)-3×HA-mCMV-EGFP-Neo
Alias: NM_009910.3;Cd183; Cmkar3
Gene lenght: 1101
Host: Mammalian cells
Use(s): Gene expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Kan
Screening marker: Neo/G418
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-Cxcr3-3×HA-mCMV-EGFP-Neo plasmid Description

pCMV-Cxcr3-3×HA-mCMV-EGFP-Neo It is a plasmid suitable for gene expression in mammalian cells.

pCMV-Cxcr3-3×HA-mCMV-EGFP-Neo plasmid Sequence

1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc GCTAGCGCTA 601 CCGGACTCAG ATCTCGAGCT CCGAATTCgc caccatgtac cttgaggtta gtgaacgtca 661 agtgctagat gcctcggact ttgcctttct tctggaaaac agcacctctc cctacgatta 721 tggggaaaac gagagcgact tctctgactc cccgccctgc ccacaggatt tcagcctgaa 781 ctttgacaga accttcctgc cagccctcta cagcctcctc ttcttgctgg ggctgctagg 841 caatggggcg gtggctgctg tgctactgag tcagcgcact gccctgagca gcacggacac 901 cttcctgctc cacctggctg tagccgatgt tctgctggtg ttaactcttc cattgtgggc 961 agtggatgct gctgtccagt gggttttcgg ccctggcctc tgcaaagtgg caggcgcctt 1021 gttcaacatc aacttctatg caggggcctt cctgctggct tgtataagct tcgacagata 1081 tctgagcata gtgcacgcca cccagatcta ccgcagggac ccccgggtac gtgtagccct 1141 cacctgcata gttgtatggg gtctctgtct gctctttgcc ctcccagatt tcatctacct 1201 atcagccaac tacgatcagc gcctcaatgc cacccattgc cagtacaact tcccacaggt 1261 gggtcgcact gctctgcgtg tactgcagct agtggctggt ttcctgctgc cccttctggt 1321 catggcctac tgctatgccc atatcctagc tgttctgctg gtctccagag gccagaggcg 1381 ttttcgagct atgaggctag tggtagtggt ggtggcagcc tttgctgtct gctggacccc 1441 ctatcacctg gtggtgctag tggatatcct catggatgtg ggagttttgg cccgcaactg 1501 tggtcgagaa agccacgtgg atgtggccaa gtcagtcacc tcgggcatgg ggtacatgca 1561 ctgctgcctc aatccgctgc tctatgcctt tgtgggagtg aagttcagag agcaaatgtg 1621 gatgttgttc acgcgcctgg gccgctctga ccagagaggg ccccagcggc agccgtcatc 1681 ttcacggaga gaatcatcct ggtctgagac aactgaggcc tcctacctgg gcttgAAGCT 1741 TTACCCATAC GACGTACCTG ATTATGCAAC TTATCCTTAT GATGTACCTG ATTATGCTAG 1801 CTATCCTTAT GATGTCCCAG ACTACGCATA GGATCCGGGC CCGTTTAAAC CCGCTGATCA 1861 GCCTCGACTG TGCCTTCTAG TTGCCAGCCA TCTGTTGTTT GCCCCTCCCC CGTGCCTTCC 1921 TTGACCCTGG AAGGTGCCAC TCCCACTGTC CTTTCCTAAT AAAATGAGGA AATTGCATCG 1981 CATTGTCTGA GTAGGTGTCA TTCTATTCTG GGGGGTGGGG TGGGGCAGGA CAGCAAGGGG 2041 GAGGATTGGG AAGACAATAG CAGGCATGCT GGGGATGCGG TGGGCTCTAT GGTCTAGAGA 2101 TATACTGAGT CATTAGGGAC TTTCCAATGG GTTTTGCCCA GTACATAAGG TCAATAGGGG 2161 TGAATCAACA GGAAAGTCCC ATTGGAGCCA AGTACACTGA GTCAATAGGG ACTTTCCATT 2221 GGGTTTTGCC CAGTACAAAA GGTCAATAGG GGGTGAGTCA ATGGGTTTTT CCCATTATTG 2281 GCACGTACAT AAGGTCAATA GGGGTGAGTC ATTGGGTTTT TCCAGCCAaT TTAATTAAAA 2341 CGCCATGTAC TTTCCCACCA TTGACGTCAA TGGGCTATTG AAACTAATGC AACGTGACCT 2401 TTAAACGGTA CTTTCCCATA GCTGATTAAT GGGAAAGTAC CGTTCTCGAG CCAATACACG 2461 TCAATGGGAA GTGAAAGGGC AGCCAAAACG TAACACCGCC CCGGTTTTCC CCTGGAAATT 2521 CCATATTGGC ACGCATTCTA TTGGCTGAGC TGCGTTCTAC GTGGGTATAA GAGGCGCGAC 2581 CAGCGTCGGT ACCGTCGCAG TCTTCGGTCT GACCACCGTA GAACGCAGAT Caccggtcgc 2641 caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct 2701 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac 2761 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc 2821 caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat 2881 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat 2941 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac 3001 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg 3061 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa 3121 gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct 3181 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa 3241 ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat 3301 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa 3361 gtaaagcggc cgcgactcta gatcataatc agccatacca catttgtaga ggttttactt 3421 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt 3481 gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat 3541 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat 3601 gtatcttaag gcgtaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg 3661 ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa 3721 agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa 3781 gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg 3841 tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa 3901 ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa 3961 ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct 4021 gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt caggtggcac 4081 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat 4141 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag 4201 tcctgaggcg gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca 4261 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt 4321 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca 4381 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc 4441 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg 4501 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa 4561 gatcgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc 4621 aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat 4681 cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt 4741 caagaccgac ctgtccggtg ccctgaatga actgcaagac gaggcagcgc ggctatcgtg 4801 gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag 4861 ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc 4921 tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc 4981 tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga 5041 agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga 5101 actgttcgcc aggctcaagg cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg 5161 cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg 5221 tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc 5281 tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc 5341 cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg 5401 gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc 5461 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc 5521 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ctagggggag gctaactgaa 5581 acacggaagg agacaatacc ggaaggaacc cgcgctatga cggcaataaa aagacagaat 5641 aaaacgcacg gtgttgggtc gtttgttcat aaacgcgggg ttcggtccca gggctggcac 5701 tctgtcgata ccccaccgag accccattgg ggccaatacg cccgcgtttc ttccttttcc 5761 ccaccccacc ccccaagttc gggtgaaggc ccagggctcg cagccaacgt cggggcggca 5821 ggccctgcca tagcctcagg ttactcatat atactttaga ttgatttaaa acttcatttt 5881 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa 5941 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga 6001 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg 6061 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc 6121 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag 6181 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc 6241 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg 6301 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac 6361 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga 6421 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt 6481 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag 6541 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg 6601 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta 6661 tcccctgatt ctgtggataa ccgtattacc gccatgcat

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.