pLKO.1-U6-SMN1-shRNA3-mCherry-

pLKO.1-U6-SMN1-shRNA3-mCherry-Puro plasmid

PVT111447

pLKO.1-U6-SMN1-shRNA3-mCherry-Puro plasmid Information

Plasmid name: pLKO.1-U6-SMN1(human)-shRNA3-mCherry-Puro
Alias: GGTTGCATTTACCCAGCTACC
Gene lenght:
Host: Mammalian cells,lentivirus
Use(s): Gene interference
Fragment type: shRNA
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Red
Promoter: U6
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: U6-F(Hairpin structure)
Reverse primer:
Copy number: High
Induction:

pLKO.1-U6-SMN1-shRNA3-mCherry-Puro plasmid Description

pLKO.1-U6-SMN1-shRNA3-mCherry-Puro This plasmid is suitable for gene interference in mammalian cells and can also be used for lentivirus packaging.

pLKO.1-U6-SMN1-shRNA3-mCherry-Puro plasmid Sequence

1 tccATGGTGA GCAAGGGCGA GGAGGATAAC ATGGCCATCA TCAAGGAGTT CATGCGCTTC 61 AAGGTGCACA TGGAGGGCTC CGTGAACGGC CACGAGTTCG AGATCGAGGG CGAGGGCGAG 121 GGCCGCCCCT ACGAGGGCAC CCAGACCGCC AAGCTGAAGG TGACCAAGGG TGGCCCCCTG 181 CCCTTCGCCT GGGACATCCT GTCCCCTCAG TTCATGTACG GCTCCAAGGC CTACGTGAAG 241 CACCCCGCCG ACATCCCCGA CTACTTGAAG CTGTCCTTCC CCGAGGGCTT CAAGTGGGAG 301 CGCGTGATGA ACTTCGAGGA CGGCGGCGTG GTGACCGTGA CCCAGGACTC CTCCCTGCAG 361 GACGGCGAGT TCATCTACAA GGTGAAGCTG CGCGGCACCA ACTTCCCCTC CGACGGCCCC 421 GTAATGCAGA AGAAGACCAT GGGCTGGGAG GCCTCCTCCG AGCGGATGTA CCCCGAGGAC 481 GGCGCCCTGA AGGGCGAGAT CAAGCAGAGG CTGAAGCTGA AGGACGGCGG CCACTACGAC 541 GCTGAGGTCA AGACCACCTA CAAGGCCAAG AAGCCCGTGC AGCTGCCCGG CGCCTACAAC 601 GTCAACATCA AGTTGGACAT CACCTCCCAC AACGAGGACT ACACCATCGT GGAACAGTAC 661 GAACGCGCCG AGGGCCGCCA CTCCACCGGC GGCATGGACG AGCTGTACAA GGAATTTGGC 721 AGCGGCCAGT GCACCAACTA TGCCCTGCTG AAACTGGCTG GCGATGTGGA AAGCAACCCC 781 GGACCCatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg 841 gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat 901 ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg 961 ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg 1021 ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg 1081 agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc 1141 aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt 1201 ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc 1261 ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc 1321 accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt 1381 gcctgacgcc cgccccacga cccgcagcgc ccgaccgaaa ggagcgcacg accccatgca 1441 tcggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa 1501 gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct 1561 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg 1621 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt 1681 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc 1741 tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga 1801 atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat 1861 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc 1921 aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca 1981 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt 2041 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag 2101 gcttttttgg aggcctaggg acgtacccaa ttcgccctat agtgagtcgt attacgcgcg 2161 ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa 2221 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga 2281 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc 2341 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct 2401 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg 2461 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga 2521 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt 2581 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg 2641 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc 2701 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat 2761 attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt 2821 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg 2881 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt 2941 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta 3001 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc 3061 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa 3121 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc 3181 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt 3241 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact 3301 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac 3361 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata 3421 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta 3481 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg 3541 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat 3601 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt 3661 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga 3721 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa 3781 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag 3841 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac 3901 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc 3961 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat 4021 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat 4081 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct 4141 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt 4201 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg 4261 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta 4321 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg 4381 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg 4441 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc 4501 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg 4561 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat 4621 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc 4681 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg 4741 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt 4801 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt 4861 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac 4921 agctatgacc atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg 4981 gagctgcaag cttaatgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga 5041 tgagttagca acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag 5101 taaggtggta cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac 5161 gaaccactga attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa 5221 acgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc 5281 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt 5341 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta 5401 gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg 5461 caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac 5521 gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat 5581 taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa 5641 aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa 5701 tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc 5761 ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg 5821 tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga 5881 gcaaaacaaa agtaagacca ccgcacagca agcggccgct gatcttcaga cctggaggag 5941 gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac 6001 cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag 6061 tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag 6121 cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga 6181 acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca 6241 tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc 6301 tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta 6361 gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca 6421 gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc 6481 aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt 6541 ttaacataac aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg 6601 taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt 6661 caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa 6721 tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg aacggatctc 6781 gacggtatcg atcacgagac tagcctcgag cggccgcccc cttcaccgag ggcctatttc 6841 ccatgattcc ttcatatttg catatacgat acaaggctgt tagagagata attggaatta 6901 atttgactgt aaacacaaag atattagtac aaaatacgtg acgtagaaag taataatttc 6961 ttgggtagtt tgcagtttta aaattatgtt ttaaaatgga ctatcatatg cttaccgtaa 7021 cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga aacACCGGGG 7081 TTGCATTTAC CCAGCTACCT TCAAGAGAGG TAGCTGGGTA AATGCAACCt tttttGAATT 7141 Ctcgacctcg agacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg 7201 ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga 7261 attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga 7321 tccactttgg ccgcggctcg agggggttgg ggttgcgcct tttccaaggc agccctgggt 7381 ttgcgcaggg acgcggctgc tctgggcgtg gttccgggaa acgcagcggc gccgaccctg 7441 ggtctcgcac attcttcacg tccgttcgca gcgtcacccg gatcttcgcc gctacccttg 7501 tgggcccccc ggcgacgctt cctgctccgc ccctaagtcg ggaaggttcc ttgcggttcg 7561 cggcgtgccg gacgtgacaa acggaagccg cacgtctcac tagtaccctc gcagacggac 7621 agcgccaggg agcaatggca gcgcgccgac cgcgatgggc tgtggccaat agcggctgct 7681 cagcagggcg cgccgagagc agcggccggg aaggggcggt gcgggaggcg gggtgtgggg 7741 cggtagtgtg ggccctgttc ctgcccgcgc ggtgttccgc attctgcaag cctccggagc 7801 gcacgtcggc agtcggctcc ctcgttgacc gaatcaccga cctctctccc caggggga

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.