pLV3-CMV-NUMA1-3×FLAG-Puro pla

pLV3-CMV-NUMA1-3×FLAG-Puro plasmid

PVT111429

pLV3-CMV-NUMA1-3×FLAG-Puro plasmid Information

Plasmid name: pLV3-CMV-NUMA1(human-toxic-opt)-3×FLAG-Puro
Alias: NM_006185.4;NMP-22; NUMA
Gene lenght: 6345
Host: Mammalian cells,lentivirus
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-NUMA1-3×FLAG-Puro plasmid Description

pLV3-CMV-NUMA1-3×FLAG-Puro is a plasmid suitable for gene expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-NUMA1-3×FLAG-Puro plasmid Sequence

1 ACGCGTGTAG TCTTATGCAA TACTCTTGTA GTCTTGCAAC ATGGTAACGA TGAGTTAGCA 61 ACATGCCTTA CAAGGAGAGA AAAAGCACCG TGCATGCCGA TTGGTGGAAG TAAGGTGGTA 121 CGATCGTGCC TTATTAGGAA GGCAACAGAC GGGTCTGACA TGGATTGGAC GAACCACTGA 181 ATTGCCGCAT TGCAGAGATA TTGTATTTAA GTGCCTAGCT CGATACAATA AACGGGTCTC 241 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 301 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 361 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 421 CCCGAACAGG GACCTGAAAG CGAAAGGGAA ACCAGAGCTC TCTCGACGCA GGACTCGGCT 481 TGCTGAAGCG CGCACGGCAA GAGGCGAGGG GCGGCGACTG GTGAGTACGC CAAAAATTTT 541 GACTAGCGGA GGCTAGAAGG AGAGAGATGG GTGCGAGAGC GTCAGTATTA AGCGGGGGAG 601 AATTAGATCG CGATGGGAAA AAATTCGGTT AAGGCCAGGG GGAAAGAAAA AATATAAATT 661 AAAACATATA GTATGGGCAA GCAGGGAGCT AGAACGATTC GCAGTTAATC CTGGCCTGTT 721 AGAAACATCA GAAGGCTGTA GACAAATACT GGGACAGCTA CAACCATCCC TTCAGACAGG 781 ATCAGAAGAA CTTAGATCAT TATATAATAC AGTAGCAACC CTCTATTGTG TGCATCAAAG 841 GATAGAGATA AAAGACACCA AGGAAGCTTT AGACAAGATA GAGGAAGAGC AAAACAAAAG 901 TAAGACCACC GCACAGCAAG CGGCCACTGA TCTTCAGACC TGGAGGAGGA GATATGAGGG 961 ACAATTGGAG AAGTGAATTA TATAAATATA AAGTAGTAAA AATTGAACCA TTAGGAGTAG 1021 CACCCACCAA GGCAAAGAGA AGAGTGGTGC AGAGAGAAAA AAGAGCAGTG GGAATAGGAG 1081 CTTTGTTCCT TGGGTTCTTG GGAGCAGCAG GAAGCACTAT GGGCGCAGCC TCAATGACGC 1141 TGACGGTACA GGCCAGACAA TTATTGTCTG GTATAGTGCA GCAGCAGAAC AATTTGCTGA 1201 GGGCTATTGA GGCGCAACAG CATCTGTTGC AACTCACAGT CTGGGGCATC AAGCAGCTCC 1261 AGGCAAGAAT CCTGGCTGTG GAAAGATACC TAAAGGATCA ACAGCTCCTG GGGATTTGGG 1321 GTTGCTCTGG AAAACTCATT TGCACCACTG CTGTGCCTTG GAATGCTAGT TGGAGTAATA 1381 AATCTCTGGA ACAGATTGGA ATCACACGAC CTGGATGGAG TGGGACAGAG AAATTAACAA 1441 TTACACAAGC TTAATACACT CCTTAATTGA AGAATCGCAA AACCAGCAAG AAAAGAATGA 1501 ACAAGAATTA TTGGAATTAG ATAAATGGGC AAGTTTGTGG AATTGGTTTA ACATAACAAA 1561 TTGGCTGTGG TATATAAAAT TATTCATAAT GATAGTAGGA GGCTTGGTAG GTTTAAGAAT 1621 AGTTTTTGCT GTACTTTCTA TAGTGAATAG AGTTAGGCAG GGATATTCAC CATTATCGTT 1681 TCAGACCCAC CTCCCAACCC CGAGGGGACC CGACAGGCCC GAAGGAATAG AAGAAGAAGG 1741 TGGAGAGAGA GACAGAGACA GATCCATTCG ATTAGTGAAC GGATCTCGAC GGTATCGGTT 1801 AACTTTTAAA AGAAAAGGGG GGATTGGGGG GTACAGTGCA GGGGAAAGAA TAGTAGACAT 1861 AATAGCAACA GACATACAAA CTAAAGAATT ACAAAAACAA ATTACAAAAT TCAAAATTTT 1921 ATCGATACTA GTATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA 1981 TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC 2041 GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA 2101 GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT 2161 TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTTTA TATAAGCAGA GCTCGTTTAG 2221 TGAACCGTCA GATCGCCTGG AGACGCCATC CACGCTGTTT TGACCTCCAT AGAAGATTCT 2281 AGAGCTAGCG AATTCGCCAC Catgacactc cacgccaccc ggggggctgc actcctctct 2341 tgggtgaaca gtctacacgt ggctgaccct gtggaggctg tgctgcagct ccaggactgc 2401 agcatcttca tcaagatcat tgacagaatc catggcactg aagagggaca gcaaatcttg 2461 aagcagccgg tgtcagagag actggacttt gtgtgcagtt ttctgcagaa aaatcgaaaa 2521 catccctctt ccccagaatg cctggtatct gcacagaagg tgctagaggg atcagagctg 2581 gaactggcga agatgaccat gctgctctta taccactcta ccatgagctc caaaagtccc 2641 agggactggg aacagtttga atataaaatt caggctgagt tggctgtcat tcttaaattt 2701 gtgctggacc atgaggacgg gctaaacctt aatgaggacc tagagaactt cctacagaaa 2761 gctcctgtgc cttctacctg ttctagcaca ttccctgaag agctctcccc acctagccac 2821 caggccaaga gggagattcg cttcctagag ctacagaagg ttgcctcctc ttccagtggg 2881 aacaactttc tctcaggttc tccagcttct cccatgggtg atatcctgca gaccccacag 2941 ttccagatga gacggctgaa gaagcagctt gctgatgaga gaagtaatag ggatgagctg 3001 gagctggagc tagctgagaa ccgcaagctc ctcaccgaga aggatgcaca gatagccatg 3061 atgcagcagc gcattgaccg cctagccctg ctgaatgaga agcaggcggc cagcccactg 3121 gagcccaagg agcttgagga gctgcgtgac aagaatgaga gccttaccat gcggctgcat 3181 gaaaccctga agcagtgcca ggacctgaag acagagaaga gccagatgga tcgcaaaatc 3241 aaccagcttt cggaggagaa tggagacctt tcctttaagc tgcgggagtt tgccagtcat 3301 ctgcagcagc tacaggatgc cctcaatgag ctgacggagg agcacagcaa ggccactcag 3361 gagtggctag agaagcaggc ccagctggag aaggagctca gcgcagccct gcaggacaag 3421 aaatgccttg aagagaagaa cgaaatcctt cagggaaaac tttcacagct ggaagaacac 3481 ttgtcccagc tgcaggataa cccaccccag gagaagggcg aggtgctggg tgatgtcttg 3541 cagctggaaa ccttgaagca agaggcagcc actcttgctg caaacaacac acagctccaa 3601 gccagggtag agATGCTGGA GACCGAGCGC GGCCAGCAGG AGGCCAAGCT GCTGGCCGAG 3661 CGCGGCCACT TCGAGGAGGA GAAGCAGCAG CTGAGCAGCC TGATCACCGA CCTGCAGAGC 3721 AGCATCAGCA ACCTGAGCCA GGCCAAGGAG GAGCTGGAGC AGGCCAGCCA GGCCCACGGC 3781 GCCCGCCTGA CCGCCCAGGT GGCCAGCCTG ACCAGCGAGC TGACCACCCT GAACGCCACC 3841 ATCCAGCAGC AGGACCAGGA GCTGGCCGGC CTGAAGCAGC AGGCCAAGGA GAAGCAGGCC 3901 CAGCTGGCCC AGACCCTGCA GCAGCAGGAG CAGGCCAGCC AGGGCCTGCG CCACCAGGTG 3961 GAGCAGCTGA GCAGCAGCCT GAAGCAGAAG GAGCAGCAGC TGAAGGAGGT GGCCGAGAAG 4021 CAGGAGGCCA CCCGCCAGGA CCACGCCCAG CAGCTGGCCA CCGCCGCCGA GGAGCGCGAG 4081 GCCAGCCTGC GCGAGCGCGA CGCCGCCCTG AAGCAGCTGG AGGCCCTGGA GAAGGAGAAG 4141 GCCGCCAAGC TGGAGATCCT GCAGCAGCAG CTGCAGGTGG CCAACGAGGC CCGCGACAGC 4201 GCCCAGACCA GCGTGACCCA GGCCCAGCGC GAGAAGGCCG AGCTGAGCCG CAAGGTGGAG 4261 GAGCTGCAGG CCTGCGTGGA GACCGCCCGC CAGGAGCAGC ACGAGGCCCA GGCCCAGGTG 4321 GCCGAGCTGG AGCTGCAGCT GCGCAGCGAG CAGCAGAAGG CCACCGAGAA GGAGCGCGTG 4381 GCCCAGGAGA AGGACCAGCT GCAGGAGCAG CTGCAGGCCC TGAAGGAGAG CCTGAAGGTG 4441 ACCAAGGGCA GCCTGGAGGA GGAGAAGCGC CGCGCCGCCG ACGCCCTGGA GGAGCAGCAG 4501 CGCTGCATCA GCGAGCTGAA GGCCGAGACC CGCAGCCTGG TGGAGCAGCA CAAGCGCGAG 4561 CGCAAGGAGC TGGAGGAGGA GCGCGCCGGC CGCAAGGGCC TGGAGGCCCG Cttacagcag 4621 cttggggagg cccatcaggc tgagactgaa gtcctgcggc gggagctggc agaggccatg 4681 gctgcccagc acacagctga gagtgagtgt gagcagctcg tcaaagaagt agctgcctgg 4741 cgtgagcggt atgaggatag ccagcaagag gaggcacagt atggcgccat gttccaggaa 4801 cagctgatga ctttgaagga ggaatgtgag aaggcccgcc aggagctgca ggaggcaaag 4861 gagaaggtgg caggcataga atcccacagc gagctccaga taagccggca gcagaacgaa 4921 ctagctgagc tccatgccaa cctggccaga gcactccagc aggtccaaga gaaggaagtc 4981 agggcccaga agcttgcaga tgacctctcc actctgcagg aaaagatggc tgccaccagc 5041 aaagaggtgg cccgcttgga gaccttggtg cgcaaggcag gtgagcagca ggaaacagcc 5101 tcccgggagt tagtcaagga gcctgcgagg gcaggagaca gacagcccga gtggctggaa 5161 gagcaacagg gacgccagtt ctgcagcaca caggcagcgc tgcaggctat ggagcgggag 5221 gcagagcaga tgggcaatga gctggaacgg ctgcgggccg cgctgatgga gagccagggg 5281 cagcagcagg aggagcgtgg gcagcaggaa agggaggtgg cgcggctgac ccaggagcgg 5341 ggccgtgccc aggctgacct tgccctggag aaggcggcca gagcagagct tgagatgcgg 5401 ctgcagaacg ccctcaacga gcagcgtgtg gagttcgcta ccctgcaaga ggcactggct 5461 catgccctga cggaaaagga aggcaaggac caggagttgg ccaagcttcg tggtctggag 5521 gcagcccaga taaaagagct ggaggaactt cggcaaaccg tgaagcaact gaaggaacag 5581 ctggctaaga aagaaaagga gcacgcatct ggctcaggag cccaatctga ggctgctggc 5641 aggacagagc caacaggccc caagctggag gcactgcggg cagaggtgag caagctggaa 5701 cagcaatgcc agaagcagca ggagcaggct gacagcctgg aacgcagcct cgaggctgag 5761 cgggcctccc gggctgagcg ggacagtgct ctggagactc tgcagggcca gttagaggag 5821 aaggcccagg agctagggca cagtcagagt gccttagcct cggcccaacg ggagttggct 5881 gccttccgca ccaaggtaca agaccacagc aaggctgaag atgagtggaa ggcccaggtg 5941 gcccggggcc ggcaagaggc tgagaggaaa aatagcctca tcagcagctt ggaggaggag 6001 gtgtccatcc tgaatcgcca ggtcctggag aaggaggggg agagcaagga gttgaagcgg 6061 ctggtgatgg ccgagtcaga gaagagccag aagctggagg agaggctgcg cctgctgcag 6121 gcagagacag ccagcaacag tgccagagct gcagaacgca gctctgctct gcgggaggag 6181 gtgcagagcc tccgggagga ggctgagaaa cagcgggtgg cttcagagaa cctgcggcag 6241 gagctgacct cacaggctga gcgtgcggag gagctgggcc aagaattgaa ggcgtggcag 6301 gagaagttct tccagaaaga gcaggccctc tccaccctgc agctcgagca caccagcaca 6361 caggccctgg tgagtgagct gctgccagct aagcacctct gccagcagct gcaggccgag 6421 caggccgctg ccgagaaacg ccaccgtgag gagctggagc agagcaagca ggccgctggg 6481 ggactgcggg cagagctgct gcgggcccag cgggagcttg gggagctgat tcctctgcgg 6541 cagaaggtgg cagagcagga gcgaacagct cagcagctgc gggcagagaa ggccagctat 6601 gcagagcagc tgagcatgct gaagaaggcg catggcctgc tggcagagga gaaccggggg 6661 ctgggtgagc gggccaacct tggccggcag tttctggaag tggagttgga ccaggcccgg 6721 gagaagtatg tccaagagtt ggcagccgta cgtgctgatg ctgagacccg tctggctgag 6781 gtgcagcgag aagcacagag cactgcccgg gagctggagg tgatgactgc caagtatgag 6841 ggtgccaagg tcaaggtcct ggaggagagg cagcggttcc aggaagagag gcagaaactc 6901 actgcccagg tggagcagct agaggtattt cagagagagc aaactaagca ggtggaagaa 6961 ctgagtaaga aactggctga ctctgaccaa gccagcaagg tgcagcagca gaagctgaag 7021 gctgtccagg ctcagggagg cgagagccag caggaggccc agcgcctcca ggcccagctg 7081 aatgaactgc aagcccagtt gagccagaag gagcaggcag ctgagcacta taagctgcag 7141 atggagaaag ccaaaacaca ttatgatgcc aagaagcagc agaaccaaga gctgcaggag 7201 cagctgcgga gcctggagca gctgcagaag gaaaacaaag agctgcgagc tgaagctgaa 7261 cggctgggcc atgagctaca gcaggctggg ctgaagacca aggaggctga acagacctgc 7321 cgccacctta ctgcccaggt gcgcagcctg gaggcacagg ttgcccatgc agaccagcag 7381 cttcgagacc tgggcaaatt ccaggtggca actgatgctt taaagagccg tgagccccag 7441 gctaagcccc agctggactt gagtattgac agcctggatc tgagctgcga ggaggggacc 7501 ccactcagta tcaccagcaa gctgcctcgt acccagccag acggcaccag cgtccctgga 7561 gaaccagcct cacctatctc ccagcgcctg ccccccaagg tagaatccct ggagagtctc 7621 tacttcactc ccatccctgc tcggagtcag gcccccctgg agagcagcct ggactccctg 7681 ggagacgtct tcctggactc gggtcgtaag acccgctccg ctcgtcggcg caccacgcag 7741 atcatcaaca tcaccatgac caagaagcta gatgtggaag agccagacag cgccaactca 7801 tcgttctaca gcacgcggtc tgctcctgct tcccaggcta gcctgcgagc cacctcctct 7861 actcagtctc tagctcgcct gggttctccc gattatggca actcagccct gctcagcttg 7921 cctggctacc gccccaccac tcgcagttct gctcgtcgtt cccaggccgg ggtgtccagt 7981 ggggcccctc caggaaggaa cagcttctac atgggcactt gccaggatga gcctgagcag 8041 ctggatgact ggaaccgcat tgcagagctg cagcagcgca atcgagtgtg ccccccacat 8101 ctgaagacct gctatcccct ggagtccagg ccttccctga gcctgggcac catcacagat 8161 gaggagatga aaactggaga cccccaagag accctgcgcc gagccagcat gcagccaatc 8221 cagatagccg agggcactgg catcaccacc cggcagcagc gcaaacgggt ctccctagag 8281 ccccaccagg gccctggaac tcctgagtct aagaaggcca ccagctgttt cccacgcccc 8341 atgactcccc gagaccgaca tgaagggcgc aaacagagca ctactgaggc ccagaagaaa 8401 gcagctccag cttctactaa acaggctgac cggcgccagt cgatggcctt cagcatcctc 8461 aacacaccca agaagctagg gaacagcctt ctgcggcggg gagcctcaaa gaaggccctg 8521 tccaaggctt cccccaacac tcgcagtgga acccgccgtt ctccgcgcat tgccaccacc 8581 acagccagcg ccgccactgc tgccgccatt ggtgccaccc ctcgagccaa gggcaaggca 8641 aagcacGGAT CCGACTACAA AGACCATGAC GGTGATTATA AAGATCATGA CATCGACTAC 8701 AAGGATGACG ATGACAAGTA GCGGCCGCGA AGGATCTGCG ATCGCTCCGG TGCCCGTCAG 8761 TGGGCAGAGC GCACATCGCC CACAGTCCCC GAGAAGTTGG GGGGAGGGGT CGGCAATTGA 8821 ACGGGTGCCT AGAGAAGGTG GCGCGGGGTA AACTGGGAAA GTGATGTCGT GTACTGGCTC 8881 CGCCTTTTTC CCGAGGGTGG GGGAGAACCG TATATAAGTG CAGTAGTCGC CGTGAACGTT 8941 CTTTTTCGCA ACGGGTTTGC CGCCAGAACA CAGCTGAAGC TTCGAGGGGC TCGCATCTCT 9001 CCTTCACGCG CCCGCCGCCC TACCTGAGGC CGCCATCCAC GCCGGTTGAG TCGCGTTCTG 9061 CCGCCTCCCG CCTGTGGTGC CTCCTGAACT GCGTCCGCCG TCTAGGTAAG TTTAAAGCTC 9121 AGGTCGAGAC CGGGCCTTTG TCCGGCGCTC CCTTGGAGCC TACCTAGACT CAGCCGGCTC 9181 TCCACGCTTT GCCTGACCCT GCTTGCTCAA CTCTACGTCT TTGTTTCGTT TTCTGTTCTG 9241 CGCCGTTACA GATCCAAGCT GTGACCGGCG CCTACGCTAG ATGACCGAGT ACAAGCCCAC 9301 GGTGCGCCTC GCCACCCGCG ACGACGTCCC CCGGGCCGTA CGCACCCTCG CCGCCGCGTT 9361 CGCCGACTAC CCCGCCACGC GCCACACCGT CGACCCGGAC CGCCACATCG AGCGGGTCAC 9421 CGAGCTGCAA GAACTCTTCC TCACGCGCGT CGGGCTCGAC ATCGGCAAGG TGTGGGTCGC 9481 GGACGACGGC GCCGCGGTGG CGGTCTGGAC CACGCCGGAG AGCGTCGAAG CGGGGGCGGT 9541 GTTCGCCGAG ATCGGCCCGC GCATGGCCGA GTTGAGCGGT TCCCGGCTGG CCGCGCAGCA 9601 ACAGATGGAA GGCCTCCTGG CGCCGCACCG GCCCAAGGAG CCCGCGTGGT TCCTGGCCAC 9661 CGTCGGCGTC TCGCCCGACC ACCAGGGCAA GGGTCTGGGC AGCGCCGTCG TGCTCCCCGG 9721 AGTGGAGGCG GCCGAGCGCG CCGGGGTGCC CGCCTTCCTG GAGACCTCCG CGCCCCGCAA 9781 CCTCCCCTTC TACGAGCGGC TCGGCTTCAC CGTCACCGCC GACGTCGAGG TGCCCGAAGG 9841 ACCGCGCACC TGGTGCATGA CCCGCAAGCC CGGTGCCTGA GTCGACAATC AACCTCTGGA 9901 TTACAAAATT TGTGAAAGAT TGACTGGTAT TCTTAACTAT GTTGCTCCTT TTACGCTATG 9961 TGGATACGCT GCTTTAATGC CTTTGTATCA TGCTATTGCT TCCCGTATGG CTTTCATTTT 10021 CTCCTCCTTG TATAAATCCT GGTTGCTGTC TCTTTATGAG GAGTTGTGGC CCGTTGTCAG 10081 GCAACGTGGC GTGGTGTGCA CTGTGTTTGC TGACGCAACC CCCACTGGTT GGGGCATTGC 10141 CACCACCTGT CAGCTCCTTT CCGGGACTTT CGCTTTCCCC CTCCCTATTG CCACGGCGGA 10201 ACTCATCGCC GCCTGCCTTG CCCGCTGCTG GACAGGGGCT CGGCTGTTGG GCACTGACAA 10261 TTCCGTGGTG TTGTCGGGGA AATCATCGTC CTTTCCTTGG CTGCTCGCCT GTGTTGCCAC 10321 CTGGATTCTG CGCGGGACGT CCTTCTGCTA CGTCCCTTCG GCCCTCAATC CAGCGGACCT 10381 TCCTTCCCGC GGCCTGCTGC CGGCTCTGCG GCCTCTTCCG CGTCTTCGCC TTCGCCCTCA 10441 GACGAGTCGG ATCTCCCTTT GGGCCGCCTC CCCGCCTGGT ACCTTTAAGA CCAATGACTT 10501 ACAAGGCAGC TGTAGATCTT AGCCACTTTT TAAAAGAAAA GGGGGGACTG GAAGGGCTAA 10561 TTCACTCCCA ACGAAAATAA GATCTGCTTT TTGCTTGTAC TGGGTCTCTC TGGTTAGACC 10621 AGATCTGAGC CTGGGAGCTC TCTGGCTAAC TAGGGAACCC ACTGCTTAAG CCTCAATAAA 10681 GCTTGCCTTG AGTGCTTCAA GTAGTGTGTG CCCGTCTGTT GTGTGACTCT GGTAACTAGA 10741 GATCCCTCAG ACCCTTTTAG TCAGTGTGGA AAATCTCTAG CAGTAGTAGT TCATGTCATC 10801 TTATTATTCA GTATTTATAA CTTGCAAAGA AATGAATATC AGAGAGTGAG AGGAACTTGT 10861 TTATTGCAGC TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG 10921 CATTTTTTTC ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTATCATG 10981 TCTGGCTCTA GCTATCCCGC CCCTAACTCC GCCCAGTTCC GCCCATTCTC CGCCCCATGG 11041 CTGACTAATT TTTTTTATTT ATGCAGAGGC CGAGGCCGCC TCGGCCTCTG AGCTATTCCA 11101 GAAGTAGTGA GGAGGCTTTT TTGGAGGCCT AGACTTTTGC AGAGACGGCC CAAATTCGTA 11161 ATCATGGTCA TAGCTGTTTC CTGTGTGAAA TTGTTATCCG CTCACAATTC CACACAACAT 11221 ACGAGCCGGA AGCATAAAGT GTAAAGCCTG GGGTGCCTAA TGAGTGAGCT AACTCACATT 11281 AATTGCGTTG CGCTCACTGC CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC AGCTGCATTA 11341 ATGAATCGGC CAACGCGCGG GGAGAGGCGG TTTGCGTATT GGGCGCTCTT CCGCTTCCTC 11401 GCTCACTGAC TCGCTGCGCT CGGTCGTTCG GCTGCGGCGA GCGGTATCAG CTCACTCAAA 11461 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 11521 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 11581 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 11641 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 11701 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 11761 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 11821 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 11881 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 11941 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 12001 CACTAGAAGG ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 12061 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 12121 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 12181 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 12241 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 12301 TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA ATCAGTGAGG CACCTATCTC 12361 AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC CCCGTCGTGT AGATAACTAC 12421 GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG ATACCGCGAG ACCCACGCTC 12481 ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG 12541 TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG CTAGAGTAAG 12601 TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTACAGGCA TCGTGGTGTC 12661 ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA GGCGAGTTAC 12721 ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG 12781 AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA ATTCTCTTAC 12841 TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA AGTCATTCTG 12901 AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAATACGGG ATAATACCGC 12961 GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG GGCGAAAACT 13021 CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG CACCCAACTG 13081 ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG GAAGGCAAAA 13141 TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATAC TCTTCCTTTT 13201 TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG 13261 TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA 13321 CGTCTAAGAA ACCATTATTA TCATGACATT AACCTATAAA AATAGGCGTA TCACGAGGCC 13381 CTTTCGTCTC GCGCGTTTCG GTGATGACGG TGAAAACCTC TGACACATGC AGCTCCCGGA 13441 GACGGTCACA GCTTGTCTGT AAGCGGATGC CGGGAGCAGA CAAGCCCGTC AGGGCGCGTC 13501 AGCGGGTGTT GGCGGGTGTC GGGGCTGGCT TAACTATGCG GCATCAGAGC AGATTGTACT 13561 GAGAGTGCAC CATATGCGGT GTGAAATACC GCACAGATGC GTAAGGAGAA AATACCGCAT 13621 CAGGCGCCAT TCGCCATTCA GGCTGCGCAA CTGTTGGGAA GGGCGATCGG TGCGGGCCTC 13681 TTCGCTATTA CGCCAGCTGG CGAAAGGGGG ATGTGCTGCA AGGCGATTAA GTTGGGTAAC 13741 GCCAGGGTTT TCCCAGTCAC GACGTTGTAA AACGACGGCC AGTGCCAAGC TG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.