pLV2-CMV-ERBB2-P2A-FLuc-Puro p

pLV2-CMV-ERBB2-P2A-FLuc-Puro plasmid

PVT111363

pLV2-CMV-ERBB2-P2A-FLuc-Puro plasmid Information

Plasmid name: pLV2-CMV-ERBB2(human)-P2A-FLuc-Puro
Alias: NM_001005862.3;c-ERB-2; c-ERB2; CD340; HER-2; HER-2/neu;HER2; MLN 19; NEU; NGL; TKR1; VSCN2
Gene lenght: 3675
Host: Mammalian cells,lentivirus
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Fluc
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV2-CMV-ERBB2-P2A-FLuc-Puro plasmid Description

pLV2-CMV-ERBB2-P2A-FLuc-Puro It is a plasmid suitable for gene expression in mammalian cells, and is also suitable for lentivirus packaging.

pLV2-CMV-ERBB2-P2A-FLuc-Puro plasmid Sequence

1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 2821 GCCACCatgg agctggcggc cttgtgccgc tgggggctcc tcctcgccct cttgcccccc 2881 ggagccgcga gcacccaagt gtgcaccggc acagacatga agctgcggct ccctgccagt 2941 cccgagaccc acctggacat gctccgccac ctctaccagg gctgccaggt ggtgcaggga 3001 aacctggaac tcacctacct gcccaccaat gccagcctgt ccttcctgca ggatatccag 3061 gaggtgcagg gctacgtgct catcgctcac aaccaagtga ggcaggtccc actgcagagg 3121 ctgcggattg tgcgaggcac ccagctcttt gaggacaact atgccctggc cgtgctagac 3181 aatggagacc cgctgaacaa taccacccct gtcacagggg cctccccagg aggcctgcgg 3241 gagctgcagc ttcgaagcct cacagagatc ttgaaaggag gggtcttgat ccagcggaac 3301 ccccagctct gctaccagga cacgattttg tggaaggaca tcttccacaa gaacaaccag 3361 ctggctctca cactgataga caccaaccgc tctcgggcct gccacccctg ttctccgatg 3421 tgtaagggct cccgctgctg gggagagagt tctgaggatt gtcagagcct gacgcgcact 3481 gtctgtgccg gtggctgtgc ccgctgcaag gggccactgc ccactgactg ctgccatgag 3541 cagtgtgctg ccggctgcac gggccccaag cactctgact gcctggcctg cctccacttc 3601 aaccacagtg gcatctgtga gctgcactgc ccagccctgg tcacctacaa cacagacacg 3661 tttgagtcca tgcccaatcc cgagggccgg tatacattcg gcgccagctg tgtgactgcc 3721 tgtccctaca actacctttc tacggacgtg ggatcctgca ccctcgtctg ccccctgcac 3781 aaccaagagg tgacagcaga ggatggaaca cagcggtgtg agaagtgcag caagccctgt 3841 gcccgagtgt gctatggtct gggcatggag cacttgcgag aggtgagggc agttaccagt 3901 gccaatatcc aggagtttgc tggctgcaag aagatctttg ggagcctggc atttctgccg 3961 gagagctttg atggggaccc agcctccaac actgccccgc tccagccaga gcagctccaa 4021 gtgtttgaga ctctggaaga gatcacaggt tacctataca tctcagcatg gccggacagc 4081 ctgcctgacc tcagcgtctt ccagaacctg caagtaatcc ggggacgaat tctgcacaat 4141 ggcgcctact cgctgaccct gcaagggctg ggcatcagct ggctggggct gcgctcactg 4201 agggaactgg gcagtggact ggccctcatc caccataaca cccacctctg cttcgtgcac 4261 acggtgccct gggaccagct ctttcggaac ccgcaccaag ctctgctcca cactgccaac 4321 cggccagagg acgagtgtgt gggcgagggc ctggcctgcc accagctgtg cgcccgaggg 4381 cactgctggg gtccagggcc cacccagtgt gtcaactgca gccagttcct tcggggccag 4441 gagtgcgtgg aggaatgccg agtactgcag gggctcccca gggagtatgt gaatgccagg 4501 cactgtttgc cgtgccaccc tgagtgtcag ccccagaatg gctcagtgac ctgttttgga 4561 ccggaggctg accagtgtgt ggcctgtgcc cactataagg accctccctt ctgcgtggcc 4621 cgctgcccca gcggtgtgaa acctgacctc tcctacatgc ccatctggaa gtttccagat 4681 gaggagggcg catgccagcc ttgccccatc aactgcaccc actcctgtgt ggacctggat 4741 gacaagggct gccccgccga gcagagagcc agccctctga cgtccatcat ctctgcggtg 4801 gttggcattc tgctggtcgt ggtcttgggg gtggtctttg ggatcctcat caagcgacgg 4861 cagcagaaga tccggaagta cacgatgcgg agactgctgc aggaaacgga gctggtggag 4921 ccgctgacac ctagcggagc gatgcccaac caggcgcaga tgcggatcct gaaagagacg 4981 gagctgagga aggtgaaggt gcttggatct ggcgcttttg gcacagtcta caagggcatc 5041 tggatccctg atggggagaa tgtgaaaatt ccagtggcca tcaaagtgtt gagggaaaac 5101 acatccccca aagccaacaa agaaatctta gacgaagcat acgtgatggc tggtgtgggc 5161 tccccatatg tctcccgcct tctgggcatc tgcctgacat ccacggtgca gctggtgaca 5221 cagcttatgc cctatggctg cctcttagac catgtccggg aaaaccgcgg acgcctgggc 5281 tcccaggacc tgctgaactg gtgtatgcag attgccaagg ggatgagcta cctggaggat 5341 gtgcggctcg tacacaggga cttggccgct cggaacgtgc tggtcaagag tcccaaccat 5401 gtcaaaatta cagacttcgg gctggctcgg ctgctggaca ttgacgagac agagtaccat 5461 gcagatgggg gcaaggtgcc catcaagtgg atggcgctgg agtccattct ccgccggcgg 5521 ttcacccacc agagtgatgt gtggagttat ggtgtgactg tgtgggagct gatgactttt 5581 ggggccaaac cttacgatgg gatcccagcc cgggagatcc ctgacctgct ggaaaagggg 5641 gagcggctgc cccagccccc catctgcacc attgatgtct acatgatcat ggtcaaatgt 5701 tggatgattg actctgaatg tcggccaaga ttccgggagt tggtgtctga attctcccgc 5761 atggccaggg acccccagcg ctttgtggtc atccagaatg aggacttggg cccagccagt 5821 cccttggaca gcaccttcta ccgctcactg ctggaggacg atgacatggg ggacctggtg 5881 gatgctgagg agtatctggt accccagcag ggcttcttct gtccagaccc tgccccgggc 5941 gctgggggca tggtccacca caggcaccgc agctcatcta ccaggagtgg cggtggggac 6001 ctgacactag ggctggagcc ctctgaagag gaggccccca ggtctccact ggcaccctcc 6061 gaaggggctg gctccgatgt atttgatggt gacctgggaa tgggggcagc caaggggctg 6121 caaagcctcc ccacacatga ccccagccct ctacagcggt acagtgagga ccccacagta 6181 cccctgccct ctgagactga tggctacgtt gcccccctga cctgcagccc ccagcctgaa 6241 tatgtgaacc agccagatgt tcggccccag cccccttcgc cccgagaggg ccctctgcct 6301 gctgcccgac ctgctggtgc cactctggaa aggcccaaga ctctctcccc agggaagaat 6361 ggggtcgtca aagacgtttt tgcctttggg ggtgccgtgg agaaccccga gtacttgaca 6421 ccccagggag gagctgcccc tcagccccac cctcctcctg ccttcagccc agccttcgac 6481 aacctctatt actgggacca ggacccacca gagcgggggg ctccacccag caccttcaaa 6541 gggacaccta cggcagagaa cccagagtac ctgggtctgg acgtgccagt gGGATCCGGC 6601 GCAACCAATT TTAGCCTGCT GAAACAAGCT GGCGACGTTG AAGAAAATCC GGGTCCGatg 6661 gaagacgcca aaaacataaa gaaaggcccg gcgccattct atcctcttga ggatggaacc 6721 gctggagagc aactgcataa ggctatgaag agatacgccc tggttcctgg aacaattgct 6781 tttacagatg cacatatcga ggtgaacatc acgtacgcgg aatacttcga aatgtccgtt 6841 cggttggcag aagctatgaa acgatatggg ctgaatacaa atcacagaat cgtcgtatgc 6901 agtgaaaact ctcttcaatt ctttatgccg gtgttgggcg cgttatttat cggagttgca 6961 gttgcgcccg cgaacgacat ttataatgaa cgtgaattgc tcaacagtat gaacatttcg 7021 cagcctaccg tagtgtttgt ttccaaaaag gggttgcaaa aaattttgaa cgtgcaaaaa 7081 aaattaccaa taatccagaa aattattatc atggattcta aaacggatta ccagggattt 7141 cagtcgatgt acacgttcgt cacatctcat ctacctcccg gttttaatga atacgatttt 7201 gtaccagagt cctttgatcg tgacaaaaca attgcactga taatgaactc ctctggatct 7261 actgggttac ctaagggtgt ggcccttccg catagaactg cctgcgtcag attctcgcat 7321 gccagagatc ctatttttgg caatcaaatc attccggata ctgcgatttt aagtgttgtt 7381 ccattccatc acggttttgg aatgtttact acactcggat atttgatatg tggatttcga 7441 gtcgtcttaa tgtatagatt tgaagaagag ctgtttttac gatcccttca ggattacaaa 7501 attcaaagtg cgttgctagt accaacccta ttttcattct tcgccaaaag cactctgatt 7561 gacaaatacg atttatctaa tttacacgaa attgcttctg ggggcgcacc tctttcgaaa 7621 gaagtcgggg aagcggttgc aaaacgcttc catcttccag ggatacgaca aggatatggg 7681 ctcactgaga ctacatcagc tattctgatt acacccgagg gggatgataa accgggcgcg 7741 gtcggtaaag ttgttccatt ttttgaagcg aaggttgtgg atctggatac cgggaaaacg 7801 ctgggcgtta atcagagagg cgaattatgt gtcagaggac ctatgattat gtccggttat 7861 gtaaacaatc cggaagcgac caacgccttg attgacaagg atggatggct acattctgga 7921 gacatagctt actgggacga agacgaacac ttcttcatag ttgaccgctt gaagtcttta 7981 attaaataca aaggatacca ggtggccccc gctgaattgg agtcgatatt gttacaacac 8041 cccaacatct tcgacgcggg cgtggcaggt cttcccgacg atgacgccgg tgaacttccc 8101 gccgccgttg ttgttttgga gcacggaaag acgatgacgg aaaaagagat cgtggattac 8161 gtcgccagtc aagtaacaac cgcgaaaaag ttgcgcggag gagttgtgtt tgtggacgaa 8221 gtaccgaaag gtcttaccgg aaaactcgac gcaagaaaaa tcagagagat cctcataaag 8281 gccaagaagg gcggaaagtc caaattgtAG CGGCCGCtct agataattct accgggtagg 8341 ggaggcgctt ttcccaaggc agtctggagc atgcgcttta gcagccccgc tgggcacttg 8401 gcgctacaca agtggcctct ggcctcgcac acattccaca tccaccggta ggcgccaacc 8461 ggctccgttc tttggtggcc ccttcgcgcc accttctact cctcccctag tcaggaagtt 8521 cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac 8581 tagtctcgtg cagatggaca gcaccgctga gcaatggaag cgggtaggcc tttggggcag 8641 cggccaatag cagctttgct ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc 8701 cgggggcggg ctcaggggcg ggctcagggg cggggcgggc gcccgaaggt cctccggagg 8761 cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc 8821 tccgggcctt tcgacctgca gcccaagctt accatgaccg agtacaagcc cacggtgcgc 8881 ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac 8941 taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg 9001 caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac 9061 ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc 9121 gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg 9181 gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc 9241 gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag 9301 gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc 9361 ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc 9421 acctggtgca tgacccgcaa gcccggtgcc tgaccgcgtc tggaacaatc aacctctgga 9481 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg 9541 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt 9601 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag 9661 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc 9721 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga 9781 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa 9841 ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac 9901 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct 9961 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca 10021 gacgagtcgg atctcccttt gggccgcctc cccgcctgga attaattctg cagtcgagac 10081 ctagaaaaac atggagcaat cacaagtagc aatacagcag ctaccaatgc tgattgtgcc 10141 tggctagaag cacaagagga ggaggaggtg ggttttccag tcacacctca ggtaccttta 10201 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagagggga 10261 ctggaagggc taattcactc ccaacgaaga caagatatcc ttgatctgtg gatctaccac 10321 acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca 10381 ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc 10441 aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg 10501 gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga 10561 gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc 10621 gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga 10681 tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg 10741 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc 10801 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct 10861 cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat 10921 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggcctt gacattgcta 10981 gcgttttacc gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg 11041 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta 11101 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg 11161 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga 11221 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg 11281 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag 11341 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc 11401 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca 11461 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt 11521 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc 11581 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc 11641 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc 11701 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact 11761 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg 11821 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta 11881 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca 11941 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa 12001 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg 12061 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc 12121 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg 12181 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat 12241 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg 12301 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa 12361 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca 12421 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc 12481 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt 12541 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa 12601 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat 12661 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct 12721 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga 12781 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag 12841 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga 12901 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca 12961 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg 13021 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc 13081 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag 13141 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatcaac 13201 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat 13261 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat 13321 catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact tcccacccca 13381 taccctatta ccactgccaa ttacctgtgg tttcatttac tctaaacctg tgattcctct 13441 gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa cttagtagtt 13501 tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtagt

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.