pCMV-NFATC2-EGFP-SV40-Blast pl

pCMV-NFATC2-EGFP-SV40-Blast plasmid

PVT111316

pCMV-NFATC2-EGFP-SV40-Blast plasmid Information

Plasmid name: pCMV-NFATC2(human)-EGFP-SV40-Blast
Alias: NM_012340.5;NFAT1/NFATP
Gene lenght: 2763
Host: Mammalian cells
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Blast
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-NFATC2-EGFP-SV40-Blast plasmid Description

pCMV-NFATC2-EGFP-SV40-Blast It is a plasmid suitable for gene expression in mammalian cells.

pCMV-NFATC2-EGFP-SV40-Blast plasmid Sequence

1 GACGGATCGG GAGATCTCCC GATCCCCTAT GGTGCACTCT CAGTACAATC TGCTCTGATG 61 CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG 121 CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC 181 TTAGGGTTAG GCGTTTTGCG CTGCTTCGCG ATGTACGGGC CAGATATACG CGTTGACATT 241 GATTATTGAC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA 301 TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC 361 CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC 421 ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT 481 ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT 541 ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA 601 TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG 661 ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC 721 AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG 781 GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCT CTGGCTAACT AGAGAACCCA 841 CTGCTTACTG GCTTATCGAA ATTAATACGA CTCACTATAG GGAGACCCAA GCTGGCTAGC 901 AAGCTTTCTA GAGAATTCgc caccATGAAC GCCCCCGAGC GGCAGCCCCA ACCCGACGGC 961 GGGGACGCCC CAGGCCACGA GCCTGGGGGC AGCCCCCAAG ACGAGCTTGA CTTCTCCATC 1021 CTCTTCGACT ATGAGTATTT GAATCCGAAC GAAGAAGAGC CGAATGCACA TAAGGTCGCC 1081 AGCCCACCCT CCGGACCCGC ATACCCCGAT GATGTCCTGG ACTATGGCCT CAAGCCATAC 1141 AGCCCCCTTG CTAGTCTCTC TGGCGAGCCC CCCGGCCGAT TCGGAGAGCC GGATAGGGTA 1201 GGGCCGCAGA AGTTTCTGAG CGCGGCCAAG CCAGCAGGGG CCTCGGGCCT GAGCCCTCGG 1261 ATCGAGATCA CTCCGTCCCA CGAACTGATC CAGGCAGTGG GGCCCCTCCG CATGAGAGAC 1321 GCGGGCCTCC TGGTGGAGCA GCCGCCCCTG GCCGGGGTGG CCGCCAGCCC GAGGTTCACC 1381 CTGCCCGTGC CCGGCTTCGA GGGCTACCGC GAGCCGCTTT GCTTGAGCCC CGCTAGCAGC 1441 GGCTCCTCTG CCAGCTTCAT TTCTGACACC TTCTCCCCCT ACACCTCGCC CTGCGTCTCG 1501 CCCAATAACG GCGGGCCCGA CGACCTGTGT CCGCAGTTTC AAAACATCCC TGCTCATTAT 1561 TCCCCCAGAA CCTCGCCAAT AATGTCACCT CGAACCAGCC TCGCCGAGGA CAGCTGCCTG 1621 GGCCGCCACT CGCCCGTGCC CCGTCCGGCC TCCCGCTCCT CATCGCCTGG TGCCAAGCGG 1681 AGGCATTCGT GCGCCGAGGC CTTGGTTGCC CTGCCGCCCG GAGCCTCACC CCAGCGCTCC 1741 CGGAGCCCCT CGCCGCAGCC CTCATCTCAC GTGGCACCCC AGGACCACGG CTCCCCGGCT 1801 GGGTACCCCC CTGTGGCTGG CTCTGCCGTG ATCATGGATG CCCTGAACAG CCTCGCCACG 1861 GACTCGCCTT GTGGGATCCC CCCCAAGATG TGGAAGACCA GCCCTGACCC CTCGCCGGTG 1921 TCTGCCGCCC CATCCAAGGC CGGCCTGCCT CGCCACATCT ACCCGGCCGT GGAGTTCCTG 1981 GGGCCCTGCG AGCAGGGCGA GAGGAGAAAC TCGGCTCCAG AATCCATCCT GCTGGTTCCG 2041 CCCACTTGGC CCAAGCCGCT GGTGCCTGCC ATTCCCATCT GCAGCATCCC AGTGACTGCA 2101 TCCCTCCCTC CACTTGAGTG GCCGCTGTCC AGTCAGTCAG GCTCTTACGA GCTGCGGATC 2161 GAGGTGCAGC CCAAGCCACA TCACCGGGCC CACTATGAGA CAGAAGGCAG CCGAGGGGCT 2221 GTCAAAGCTC CAACTGGAGG CCACCCTGTG GTTCAGCTCC ATGGCTACAT GGAAAACAAG 2281 CCTCTGGGAC TTCAGATCTT CATTGGGACA GCTGATGAGC GGATCCTTAA GCCGCACGCC 2341 TTCTACCAGG TGCACCGAAT CACGGGGAAA ACTGTCACCA CCACCAGCTA TGAGAAGATA 2401 GTGGGCAACA CCAAAGTCCT GGAGATACCC TTGGAGCCCA AAAACAACAT GAGGGCAACC 2461 ATCGACTGTG CGGGGATCTT GAAGCTTAGA AACGCCGACA TTGAGCTGCG GAAAGGCGAG 2521 ACGGACATTG GAAGAAAGAA CACGCGGGTG AGACTGGTTT TCCGAGTTCA CATCCCAGAG 2581 TCCAGTGGCA GAATCGTCTC TTTACAGACT GCATCTAACC CCATCGAGTG CTCCCAGCGA 2641 TCTGCTCACG AGCTGCCCAT GGTTGAAAGA CAAGACACAG ACAGCTGCCT GGTCTATGGC 2701 GGCCAGCAAA TGATCCTCAC GGGGCAGAAC TTTACATCCG AGTCCAAAGT TGTGTTTACT 2761 GAGAAGACCA CAGATGGACA GCAAATTTGG GAGATGGAAG CCACGGTGGA TAAGGACAAG 2821 AGCCAGCCCA ACATGCTTTT TGTTGAGATC CCTGAATATC GGAACAAGCA TATCCGCACA 2881 CCTGTAAAAG TGAACTTCTA CGTCATCAAT GGGAAGAGAA AACGAAGTCA GCCTCAGCAC 2941 TTTACCTACC ACCCAGTCCC AGCCATCAAG ACGGAGCCCA CGGATGAATA TGACCCCACT 3001 CTGATCTGCA GCCCCACCCA TGGAGGCCTG GGGAGCCAGC CTTACTACCC CCAGCACCCG 3061 ATGGTGGCCG AGTCCCCCTC CTGCCTCGTG GCCACCATGG CTCCCTGCCA GCAGTTCCGC 3121 ACGGGGCTCT CATCCCCTGA CGCCCGCTAC CAGCAACAGA ACCCAGCGGC CGTACTCTAC 3181 CAGCGGAGCA AGAGCCTGAG CCCCAGCCTG CTGGGCTATC AGCAGCCGGC CCTCATGGCC 3241 GCCCCGCTGT CCCTTGCGGA CGCTCACCGC TCTGTGCTGG TGCACGCCGG CTCCCAGGGC 3301 CAGAGCTCAG CCCTGCTCCA CCCCTCTCCG ACCAACCAGC AGGCCTCGCC TGTGATCCAC 3361 TACTCACCCA CCAACCAGCA GCTGCGCTGC GGAAGCCACC AGGAGTTCCA GCACATCATG 3421 TACTGCGAGA ATTTCGCACC AGGCACCACC AGACCTGGCC CGCCCCCGGT CAGTCAAGGT 3481 CAGAGGCTGA GCCCGGGTTC CTACCCCACA GTCATTCAGC AGCAGAATGC CACGAGCCAA 3541 AGAGCCGCCA AAAACGGACC CCCGGTCAGT GACCAAAAGG AAGTATTACC TGCGGGGGTG 3601 ACCATTAAAC AGGAGCAGAA CTTGGACCAG ACCTACTTGG ATGATGAGCT GATAGACACA 3661 CACCTTAGCT GGATACAAAA CATATTAGGC GGTGGAGGAA GCggcggagg tggatctatg 3721 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc 3781 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc 3841 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc 3901 gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag 3961 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc 4021 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg 4081 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag 4141 ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc 4201 atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac 4261 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac 4321 ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg 4381 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaggGA 4441 TCCAGTTTAA ACCGCTGATC AGCCTCGACT GTGCCTTCTA GTTGCCAGCC ATCTGTTGTT 4501 TGCCCCTCCC CCGTGCCTTC CTTGACCCTG GAAGGTGCCA CTCCCACTGT CCTTTCCTAA 4561 TAAAATGAGG AAATTGCATC GCATTGTCTG AGTAGGTGTC ATTCTATTCT GGGGGGTGGG 4621 GTGGGGCAGG ACAGCAAGGG GGAGGATTGG GAAGACAATA GCAGGCATGC TGGGGATGCG 4681 GTGGGCTCTA TGGCTTCTGA GGCGGAAAGA ACCAGCTGGG GCTCTAGGGG GTATCCCCAC 4741 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 4801 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 4861 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 4921 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 4981 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 5041 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 5101 GGGATTTTGG CCATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 5161 GCGAATTAAT TCTGTGGAAT GTGTGTCAGT TAGGGTGTGG AAAGTCCCCA GGCTCCCCAG 5221 CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCAGGTGT GGAAAGTCCC 5281 CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA GCAACCATAG 5341 TCCCGCCCCT AACTCCGCCC ATCCCGCCCC TAACTCCGCC CAGTTCCGCC CATTCTCCGC 5401 CCCATGGCTG ACTAATTTTT TTTATTTATG CAGAGGCCGA GGCCGCCTCT GCCTCTGAGC 5461 TATTCCAGAA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA AAGCTCCCgg 5521 gATGGCCAAG CCTTTGTCTC AAGAAGAATC CACCCTCATT GAAAGAGCAA CGGCTACAAT 5581 CAACAGCATC CCCATCTCTG AAGACTACAG CGTCGCCAGC GCAGCTCTCT CTAGCGACGG 5641 CCGCATCTTC ACTGGTGTCA ATGTATATCA TTTTACTGGG GGACCTTGTG CAGAACTCGT 5701 GGTGCTGGGC ACTGCTGCTG CTGCGGCAGC TGGCAACCTG ACTTGTATCG TCGCGATCGG 5761 AAATGAGAAC AGGGGCATCT TGAGCCCCTG CGGACGGTGC CGACAGGTGC TTCTCGATCT 5821 GCATCCTGGG ATCAAAGCCA TAGTGAAGGA CAGTGATGGA CAGCCGACGG CAGTTGGGAT 5881 TCGTGAATTG CTGCCCTCTG GTTATGTGTG GGAGGGCTAA TTCGAAATGA CCGACCAAGC 5941 GACGCCCAAC CTGCCATCAC GAGATTTCGA TTCCACCGCC GCCTTCTATG AAAGGTTGGG 6001 CTTCGGAATC GTTTTCCGGG ACGCCGGCTG GATGATCCTC CAGCGCGGGG ATCTCATGCT 6061 GGAGTTCTTC GCCCACCCCA ACTTGTTTAT TGCAGCTTAT AATGGTTACA AATAAAGCAA 6121 TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG CATTCTAGTT GTGGTTTGTC 6181 CAAACTCATC AATGTATCTT ATCATGTCTG TATACCGTCG ACCTCTAGCT AGAGCTTGGC 6241 GTAATCATGG TCATAGCTGT TTCCTGTGTG AAATTGTTAT CCGCTCACAA TTCCACACAA 6301 CATACGAGCC GGAAGCATAA AGTGTAAAGC CTGGGGTGCC TAATGAGTGA GCTAACTCAC 6361 ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA AACCTGTCGT GCCAGCTGCA 6421 TTAATGAATC GGCCAACGCG CGGGGAGAGG CGGTTTGCGT ATTGGGCGCT CTTCCGCTTC 6481 CTCGCTCACT GACTCGCTGC GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT CAGCTCACTC 6541 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 6601 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 6661 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 6721 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 6781 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 6841 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 6901 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 6961 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 7021 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 7081 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 7141 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTT TTTTTGTTTG 7201 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 7261 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 7321 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 7381 TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA ATCAGTGAGG CACCTATCTC 7441 AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC CCCGTCGTGT AGATAACTAC 7501 GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG ATACCGCGAG ACCCACGCTC 7561 ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG 7621 TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG CTAGAGTAAG 7681 TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTACAGGCA TCGTGGTGTC 7741 ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA GGCGAGTTAC 7801 ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG 7861 AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA ATTCTCTTAC 7921 TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA AGTCATTCTG 7981 AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAATACGGG ATAATACCGC 8041 GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG GGCGAAAACT 8101 CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG CACCCAACTG 8161 ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG GAAGGCAAAA 8221 TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATAC TCTTCCTTTT 8281 TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG 8341 TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA 8401 CGTC

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.