pAAV-CAG-Eif2ak3-3×HA plasmid

pAAV-CAG-Eif2ak3-3×HA plasmid

PVT108792

pAAV-CAG-Eif2ak3-3×HA plasmid Information

Plasmid name: pAAV-CAG-Eif2ak3(mouse)-3×HA
Alias: NM_010121.3;Pek; Perk
Gene lenght: 3342
Host: Mammalian cells,Adeno associated virus
Use(s): Gene expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling:
Promoter: CAG
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: paavCAG-F1
Reverse primer:
Copy number: High
Induction:

pAAV-CAG-Eif2ak3-3×HA plasmid Description

pAAV-CAG-Eif2ak3-3×HA This is a plasmid suitable for protein expression in mammalian cells and can also be used for adeno-associated virus packaging.

pAAV-CAG-Eif2ak3-3×HA plasmid Sequence

1 CTTCCGCTTC CTCGCTCACT GACTCGCTGC GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT 61 CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA 121 ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT 181 TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT 241 GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC 301 GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA 361 GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT 421 CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA 481 ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG 541 GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC 601 CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA 661 CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG 721 GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT 781 TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG 841 TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA 901 AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG 961 AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG 1021 TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC 1081 GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG 1141 AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG 1201 AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG 1261 GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT 1321 CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC 1381 CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC 1441 ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA 1501 CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC 1561 GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT 1621 CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC 1681 GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA 1741 CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA 1801 TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT 1861 ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA 1921 AAGTGCCACC TAAATTGTAA GCGTTAATAT TTTGTTAAAA TTCGCGTTAA ATTTTTGTTA 1981 AATCAGCTCA TTTTTTAACC AATAGGCCGA AATCGGCAAA ATCCCTTATA AATCAAAAGA 2041 ATAGACCGAG ATAGGGTTGA GTGTTGTTCC AGTTTGGAAC AAGAGTCCAC TATTAAAGAA 2101 CGTGGACTCC AACGTCAAAG GGCGAAAAAC CGTCTATCAG GGCGATGGCC CACTACGTGA 2161 ACCATCACCC TAATCAAGTT TTTTGGGGTC GAGGTGCCGT AAAGCACTAA ATCGGAACCC 2221 TAAAGGGAGC CCCCGATTTA GAGCTTGACG GGGAAAGCCG GCGAACGTGG CGAGAAAGGA 2281 AGGGAAGAAA GCGAAAGGAG CGGGCGCTAG GGCGCTGGCA AGTGTAGCGG TCACGCTGCG 2341 CGTAACCACC ACACCCGCCG CGCTTAATGC GCCGCTACAG GGCGCGTCCC ATTCGCCATT 2401 CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC TCTTCGCTAT TACGCCAGCT 2461 GCGCGCTCGC TCGCTCACTG AGGCCGCCCG GGCAAAGCCC GGGCGTCGGG CGACCTTTGG 2521 TCGCCCGGCC TCAGTGAGCG AGCGAGCGCG CAGAGAGGGA GTGGCCAACT CCATCACTAG 2581 GGGTTCCTTG TAGTTAATGA TTAACCCGCC ATGCTACTTA TCTACGTAGC CATGCTCTAG 2641 GAAGAGTACC ATTGACGTCA ATAATGACGT ATGTTCCCAT AGTAACGCCA ATAGGGACTT 2701 TCCATTGACG TCAATGGGTG GAGTATTTAC GGTAAACTGC CCACTTGGCA GTACATCAAG 2761 TGTATCATAT GCCAAGTACG CCCCCTATTG ACGTCAATGA CGGTAAATGG CCCGCCTGGC 2821 ATTATGCCCA GTACATGACC TTATGGGACT TTCCTACTTG GCAGTACATC TACGTATTAG 2881 TCATCGCTAT TACCATGGTC GAGGTGAGCC CCACGTTCTG CTTCACTCTC CCCATCTCCC 2941 CCCCCTCCCC ACCCCCAATT TTGTATTTAT TTATTTTTTA ATTATTTTGT GCAGCGATGG 3001 GGGCGGGGGG GGGGGGGGGG CGCGCGCCGG GCGGGGCGGG GCGGGGCGAG GGGCGGGGCG 3061 GGGCGAGGCG GAGAGGTGCG GCGGCAGCCA ATCAGAGCGG CGCGCTCCGA AAGTTTCCTT 3121 TTATGGCGAG GCGGCGGCGG CGGCGGCCCT ATAAAAAGCG AAGCGCGCGG CGGGCGGGAG 3181 TCGCTGCGCG CTGCCTTCGC CCCGTGCCCC GCTCCGCCGC CGCCTCGCGC CGCCCGCCCC 3241 GGCTCTGACT GACCGCGTTA CTCCCACAGG TGAGCGGGCG GGACGGCCCT TCTCCTCCGG 3301 GCTGTAATTA GCGCTTGGTT TAATGACGGC TTGTTTCTTT TCTGTGGCTG CGTGAAAGCC 3361 TTGAGGGGCT CCGGGAGGGC CCTTTGTGCG GGGGGAGCGG CTCGGGGCTG TCCGCGGGGG 3421 GACGGCTGCC TTCGGGGGGG ACGGGGCAGG GCGGGGTTCG GCTTCTGGCG TGTGACCGGC 3481 GGCTCTAGAG CCTCTGCTAA CCATGTTCAT GCCTTCTTCT TTTTCCTACA GCTCCTGGGC 3541 AACGTGCTGG TTATTGTGCT GTCTCATCAT TTTGGCAAAG AATTGGATCC gccaccatgg 3601 agcgcgccac ccggcccggg ccgcgcgcgc tgctgctgct tctgttcctg ctgctgggct 3661 gcgcggcggg gatctcggcg gtcgcgcccg cccgcagttt gcttgctccc gcgtcggaga 3721 cagtgtttgg cttaggggca gcggccgccc cgacttcggc cgcgcgggtg cctgcggtgg 3781 caacggccga agtgaccgtg gaggacgccg aggcattgcc ggctgccgct ggcgaaccgg 3841 agtcacgcgc gacggagccc gatgacgacg tggaactgcg gcctcgcggc aggtccttgg 3901 taatcatcag cactttagat ggacgaatcg ctgcactgga tgccgagaat gatgggaaaa 3961 agcagtggga tttggacgtg gggtctggtt ccttggtttc atctagcctc agcaagccag 4021 aggtgtttgg gaacaagatg atcatcccct ccctggatgg agacctcttc cagtgggacc 4081 gggaccgaga gagcatggag gccgtcccct tcacggtgga gtccctgctc gaatcttcct 4141 acaagtttgg agatgatgtt gttctggttg gagggaaatc tctgactaca tacggactca 4201 gtgcttacag tggaaagctg aggtatatct gttctgcctt gggatgtcgc cgatgggata 4261 gtgatgaaat ggaagaagag gaagacatct tgcttctgca gcgtacgcag aagactgtgc 4321 gagctgtcgg gcctcgaagc ggcagtgaga agtggaattt cagtgttggc cactttgaac 4381 ttcggtatat tccagacatg gaaactagag ccggattcat tgaaagcacc tttaaaccgg 4441 gtggaaacaa agaagactct aaaattattt cagatgtgga agaacaagaa gccaccatgc 4501 tggacacagt gataaaagtt tccgttgctg attggaaggt catggcgttt agtaggaagg 4561 gaggccgcct ggaatgggag taccagtttt gtactcccat cgcgtccgcc tggctggtga 4621 gggatggcaa ggtcatcccc atcagcctgt ttgatgatac aagttacaca gccagcgaag 4681 aagccttggg agacgaagaa gacattgtag aggctgctcg gggagccaca gagaacagcg 4741 tgtacttagg gatgtacaga ggccagctgt acctgcagtc gtccgtcagg gtctcagaaa 4801 agttccctac aagcccaaag gccttggagt ctgtaaatgg cgaaaatgca attattcctc 4861 tgccgacgat caaatggaag cccttaatcc attctccttc taggactcct gtcttggttg 4921 ggtctgatga atttgacaaa tgtctaagta atgataagta ttcccacgaa gaatacagta 4981 atggtgcact ttcaatcctc cagtatccat acgataacgg ttactatctg ccatactaca 5041 agagagaaag gaataagcgg agcacgcaga tcacagtcag gttcctggac agcccccact 5101 acagcaagaa catccgcaag aaggacccta tcctcctgct gcactggtgg aaggagatat 5161 tcgggacgat cctgctttgc atcgtagcca cgaccttcat cgtgcgcagg cttttccatc 5221 ctcagcccca caggcagcgg aaggagtctg aaactcagtg ccagactgaa agtaaatacg 5281 actccgtgag tgccgatgtc agtgacaaca gctggaatga catgaagtac tcaggatacg 5341 tatcccgata tctaacagat tttgagccaa ttcagtgcat gggtcgtggt ggctttggcg 5401 ttgtctttga agctaaaaac aaagtagatg actgcaatta cgctatcaag aggatccggc 5461 tccccaacag ggagttggca cgggagaagg taatgcggga agttaaagcc ttggctaagc 5521 tggaacaccc aggcattgtg aggtatttca acgcctggct ggaaacccca ccagagaagt 5581 ggcaagaaga gatggatgag atctggctca aagacgaaag cacagactgg ccgctcagct 5641 cccctagccc gatggatgcc ccatctgtta agatccgaag gatggatcct ttctctacaa 5701 aagagcagat cgaagtcata gctccttctc ctgaaagaag tcggtctttc tcggtgggca 5761 tttcctgtgg ccagacaagc tcatcggaga gccagttctc tcccctggag ttctcaggga 5821 cagactgcgg agacaacagt gactcagcgg acgcagccta caacctccag gacagttgcc 5881 tgacggactg cgaggacgtg gaagatggca ccgtggacgg caatgacgag ggacactcct 5941 ttgaactttg tccgtccgaa gcttctccct atacccggtc aagggaagga acgtcctcct 6001 ccatagtgtt tgaggactct ggctgcggca acgcgtccag taaggaggag cccagaggga 6061 accggctgca tgatggcaac cattatgtta ataagctaac tgatctcaag tgctccagca 6121 gcaggtcttc ttcagaagcc accaccttgt ctacctcccc taccaggcca accactctaa 6181 gcttggattt caccaagaac actgtgggcc agctccagcc cagctccccc aaggtgtatc 6241 tgtacattca gatgcagctg tgcaggaagg agaacctcaa agactggatg aaccggcgct 6301 gcagcttgga ggaccgggag cacggcgtgt gcctgcacat cttcctgcag atcgcagagg 6361 cagtggagtt cctgcacagc aagggactca tgcacaggga cctcaagcct tccaacatat 6421 tcttcacaat ggatgatgtg gtcaaggttg gggactttgg actggtgact gctatggacc 6481 aagatgaaga agagcagact gtactgactc caatgccagc ctatgctacg cacacgggac 6541 aagtagggac caagctatac atgagcccag agcagattca tggaaacaac tactcccata 6601 aagtggacat cttctcttta ggcttgattc tgtttgaact cctctaccca ttcagcaccc 6661 agatggaacg agtccggatt ttaactgatg tcagaaatct caagtttcct ctactgttca 6721 ctcagaaata tccccaagag catatgatgg ttcaagacat gctctctcca tcccccacgg 6781 agcggcctga agccacagac atcattgaaa atgccatatt tgagaacttg gagtttcccg 6841 ggaaaacggt tctgagacag cggtcccgct ccatgagttc atctggaaca aaacattcca 6901 gacagcccag ctgctcgtac agcccactgc ctggcaacgg tggcggaggt tcaTACCCAT 6961 ACGACGTACC TGATTATGCA ACTTATCCTT ATGATGTACC TGATTATGCT AGCTATCCTT 7021 ATGATGTCCC AGACTACGCA TGAATTCGAT ATCAAGCTTA TCGATAATCA ACCTCTGGAT 7081 TACAAAATTT GTGAAAGATT GACTGGTATT CTTAACTATG TTGCTCCTTT TACGCTATGT 7141 GGATACGCTG CTTTAATGCC TTTGTATCAT GCTATTGCTT CCCGTATGGC TTTCATTTTC 7201 TCCTCCTTGT ATAAATCCTG GTTGCTGTCT CTTTATGAGG AGTTGTGGCC CGTTGTCAGG 7261 CAACGTGGCG TGGTGTGCAC TGTGTTTGCT GACGCAACCC CCACTGGTTG GGGCATTGCC 7321 ACCACCTGTC AGCTCCTTTC CGGGACTTTC GCTTTCCCCC TCCCTATTGC CACGGCGGAA 7381 CTCATCGCCG CCTGCCTTGC CCGCTGCTGG ACAGGGGCTC GGCTGTTGGG CACTGACAAT 7441 TCCGTGGTGT TGTCGGGGAA ATCATCGTCC TTTCCTTGGC TGCTCGCCTG TGTTGCCACC 7501 TGGATTCTGC GCGGGACGTC CTTCTGCTAC GTCCCTTCGG CCCTCAATCC AGCGGACCTT 7561 CCTTCCCGCG GCCTGCTGCC GGCTCTGCGG CCTCTTCCGC GTCTTCGCCT TCGCCCTCAG 7621 ACGAGTCGGA TCTCCCTTTG GGCCGCCTCC CCGCATCGAT ACCGTCGACC CGGGCGGCCG 7681 CTTCGAGCAG ACATGATAAG ATACATTGAT GAGTTTGGAC AAACCACAAC TAGAATGCAG 7741 TGAAAAAAAT GCTTTATTTG TGAAATTTGT GATGCTATTG CTTTATTTGT AACCATTATA 7801 AGCTGCAATA AACAAGTTAA CAACAACAAT TGCATTCATT TTATGTTTCA GGTTCAGGGG 7861 GAGATGTGGG AGGTTTTTTA AAGCAAGTAA AACCTCTACA AATGTGGTAA AATCGATAAG 7921 GATCTTCCTA GAGCATGGCT ACGTAGATAA GTAGCATGGC GGGTTAATCA TTAACTACAA 7981 GGAACCCCTA GTGATGGAGT TGGCCACTCC CTCTCTGCGC GCTCGCTCGC TCACTGAGGC 8041 CGGGCGACCA AAGGTCGCCC GACGCCCGGG CTTTGCCCGG GCGGCCTCAG TGAGCGAGCG 8101 AGCGCGCAGC TGcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg 8161 cgct

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.