pCMV-SP-TRIM33-TM-P2A-mCherry-

pCMV-SP-TRIM33-TM-P2A-mCherry-Neo plasmid

PVT111312

pCMV-SP-TRIM33-TM-P2A-mCherry-Neo plasmid Information

Plasmid name: pCMV-SP-TRIM33(human)-TM-P2A-mCherry-Neo
Alias: NM_015906.4;DDH4; ECTO; PTC7; RFG7; TF1G; TIF1G;TIF1GAMMA; TIFGAMMA
Gene lenght: 3381
Host: Mammalian cells
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling: Red
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-SP-TRIM33-TM-P2A-mCherry-Neo plasmid Description

pCMV-SP-TRIM33-TM-P2A-mCherry-Neo It is a plasmid suitable for gene expression in mammalian cells.

pCMV-SP-TRIM33-TM-P2A-mCherry-Neo plasmid Sequence

1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta 61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag 121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc 181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga 241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat 301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc 361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct 421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca 481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat 541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg 601 cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg 661 agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc cagcctccgg 721 actctagagc tagcgtttaa acttaagctt gAGCCACCAT GAGGGCCTGG ATCTTCTTTC 781 TCCTTTGCCT GGCCGGGAGG GCCTTGGCAC TGCCCGTCGG TACCatggcg gaaaacaaag 841 gcggcggcga ggctgagagc ggcggcgggg gcagcggcag cgcgccggta actgccgggg 901 ccgccgggcc cgccgcgcag gaggcggagc cgcctctcac cgcggtgctg gtggaggagg 961 aggaggagga aggcggcagg gccggcgctg agggcggcgc ggccgggccc gacgacgggg 1021 gggtggccgc ggcctcctcg ggctcggccc aggctgcttc atctcctgcg gcctcagtgg 1081 gcactggagt tgccgggggc gcagtatcga cgccggctcc agctccagcc tcggctcccg 1141 ctccgggtcc ctcggcaggg ccgcctcctg gaccgccagc ctcgctcctg gacacctgcg 1201 ccgtgtgtca gcagagcttg cagagccggc gtgaggcgga gcccaagctg ctgccctgtc 1261 ttcactcctt ctgcctgcgc tgcctgcccg agccggagcg ccagctcagc gtgcccatcc 1321 cggggggcag caacggcgac atccagcaag ttggtgtaat acggtgccca gtatgccgcc 1381 aagaatgcag acagatagac cttgtggata attattttgt gaaagacaca tctgaagctc 1441 ctagcagttc tgatgaaaaa tcagaacagg tatgtactag ttgtgaagac aatgcaagtg 1501 cagttggctt ttgtgtagaa tgtggagagt ggctatgtaa gacatgtatc gaagcacatc 1561 aaagagtaaa atttactaaa gatcacttga tcaggaagaa agaagatgtc tcagagtctg 1621 ttggagcatc tggtcaacgc cctgttttct gccctgtaca caaacaagaa cagttgaaac 1681 ttttctgtga aacatgtgat agattgacat gtagagactg tcagctattg gaacacaaag 1741 aacataggta tcagtttttg gaagaagctt ttcaaaatca gaagggtgca attgagaatc 1801 tactggcgaa acttcttgag aagaagaatt atgttcattt tgcagctact caggtgcaga 1861 ataggataaa agaagtaaat gagactaaca aacgagtaga acaggaaatt aaagtggcca 1921 ttttcaccct tatcaatgaa attaataaga aaggaaaatc tctcttacaa cagctagaga 1981 atgttacaaa ggaaagacag atgaagttac tacagcagca gaatgacatc acaggccttt 2041 cccggcaggt gaagcatgtt atgaacttca caaattgggc aattgcaagt ggcagcagca 2101 cagcactact atacagcaag cgactgatta ctttccagtt gcgtcatatt ttgaaagcac 2161 ggtgtgatcc tgtccctgct gctaatggag caatacgttt ccattgtgat cccaccttct 2221 gggcaaagaa tgtagtcaat ttaggtaatc tagtaataga gagtaaacca gctcctggtt 2281 atactcctaa tgttgtagtt gggcaagttc ctccagggac aaaccacatt agtaaaaccc 2341 ctggacagat taacttagca cagcttcgac tccagcacat gcaacaacaa gtatatgcac 2401 agaaacatca gcagttgcaa cagatgagga tgcagcaacc accagcacct gtaccaacta 2461 caacaacaac aacacaacag catcctagac aagcagcccc tcagatgtta caacaacagc 2521 ctcctcgatt gatcagtgtg caaacaatgc aaagaggcaa catgaactgt ggagcttttc 2581 aagcccatca gatgagactg gctcagaatg ctgccagaat accagggata cccaggcaca 2641 gcggccctca atattccatg atgcagccac acctccaaag acaacactca aacccagggc 2701 atgctggacc ctttcccgta gtatcggtac acaacaccac aatcaaccca acgagcccta 2761 ctacagcaac tatggcaaat gcaaaccgag gtcccaccag cccatctgtt acagcaatag 2821 agctaatccc ctcagttacc aatccagaaa accttccatc gctgccagat attccaccca 2881 tacagttgga agatgctggc tcaagtagtt tagataatct actaagtaga tacatctcag 2941 gcagtcacct acccccacag cctacaagca ccatgaatcc ttctccaggt ccctctgccc 3001 tttctccggg atcatcaggt ttatccaatt ctcacacacc tgtgagaccc ccaagtactt 3061 ctagtactgg cagtcgaggc agctgtgggt catcaggaag aactgctgag aagacaagtc 3121 ttagtttcaa atctgatcag gtgaaggtca agcaagaacc tgggactgaa gatgaaatat 3181 gtagcttttc aggaggtgta aaacaagaaa aaacagagga tggcaggagg agtgcttgca 3241 tgttgagcag tcctgagagt agcttgacac cacctctctc aaccaacctg catctagaaa 3301 gtgaattgga tgcattggca agcctggaaa accatgtgaa aattgaacct gcagatatga 3361 atgaaagctg caaacagtca gggctcagca gccttgttaa tggaaagtcc ccaattcgaa 3421 gcctcatgca caggtcggca aggattggag gagatggcaa caataaagat gatgacccaa 3481 atgaagactg gtgtgctgtc tgccaaaacg gaggagatct cttgtgctgc gaaaaatgtc 3541 caaaggtctt tcatctaact tgtcatgttc caacactact tagctttcca agtggggact 3601 ggatatgcac attttgtaga gatattggaa agccagaagt tgaatatgat tgtgataatt 3661 tgcaacatag taagaagggg aaaactgcgc aggggttaag ccccgtggac caaaggaaat 3721 gtgaacgtct tctgctttac ctctattgcc atgaattaag tattgaattc caggagcctg 3781 ttcctgcttc gataccaaac tactataaaa ttataaagaa accaatggat ttatccaccg 3841 tgaaaaagaa gcttcagaaa aaacattccc aacactacca aatcccggat gactttgtgg 3901 ccgatgtccg tttgatcttc aagaactgtg aaaggtttaa tgaaatgatg aaagttgttc 3961 aagtttatgc agacacacaa gagattaatt tgaaggctga ttcagaagta gctcaggcag 4021 ggaaagcagt tgcattgtac tttgaagata aactcacaga gatctactca gacaggacct 4081 tcgcaccttt gccagagttt gagcaggaag aggatgatgg tgaggtaact gaggactctg 4141 atgaagactt tatacagccc cgcagaaaac gcctaaagtc agatgagaga ccagtacata 4201 taaaggaatt cGGCTcCACC AGCGGCAGCG GCAAGCCAGG CTCCGGCGaA GGCAGCACCA 4261 AAGGCGATCT GAATgctgtg ggccaggaca cgcaggaggt catcgtggtg ccacactcct 4321 tgccctttaa ggtggtggtg atctcagcca tcctggccct ggtggtgctc accatcatct 4381 cccttatcat cctcatcatg ctttggcaga agaagccacg tATAGGGATC CGTCTCGAGT 4441 CTAGAGGGCC CTTCGGGGGT GGAGGCTCTg gaagcggagc tactaacttc agcctgctga 4501 agcaggctgg cgacgtggag gagaaccctg gacctatggt gagcaagggc gaggaggata 4561 acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg 4621 gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg 4681 ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc 4741 agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga 4801 agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg 4861 tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc 4921 tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg 4981 aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga 5041 ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca 5101 agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc 5161 acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg 5221 gcggcatgga cgagctgtac aagtaactcg agtctagagg gccccttaag ggttcgatcc 5281 ctaccggtta gtaatgagtt tgatatctcg acaatcaacc tctggattac aaaatttgtg 5341 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt 5401 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata 5461 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg 5521 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc 5581 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct 5641 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt 5701 cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg 5761 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc 5821 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct 5881 ccctttgggc cgcctccccg cctggaaacg ggggaggcta actgaaacac ggaaggagac 5941 aataccggaa ggaacccgcg ctatgacggc aataaaaaga cagaataaaa cgcacgggtg 6001 ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg ctggcactct gtcgataccc 6061 caccgagacc ccattggggc caatacgccc gcgtttcttc cttttcccca ccccaccccc 6121 caagttcggg tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag 6181 cagatctgcg cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa 6241 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc 6301 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag 6361 ctctaaatcg gggcatccct ttagggttcc gatttagtgc tttacggcac ctcgacccca 6421 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc 6481 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa 6541 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgggg atttcggcct 6601 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt 6661 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat 6721 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag 6781 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat 6841 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt 6901 tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg 6961 cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg 7021 atctgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc 7081 aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat 7141 cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt 7201 caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg 7261 gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag 7321 ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc 7381 tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc 7441 tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga 7501 agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga 7561 actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg 7621 cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg 7681 tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc 7741 tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc 7801 cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg 7861 gggttcgcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca 7921 ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga 7981 tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag 8041 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt 8101 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac 8161 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt 8221 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg 8281 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt 8341 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt 8401 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc 8461 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg 8521 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg 8581 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac 8641 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg 8701 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct 8761 ttctcccttc gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg 8821 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct 8881 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac 8941 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt 9001 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc 9061 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca 9121 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat 9181 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac 9241 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt 9301 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc 9361 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg 9421 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg 9481 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc 9541 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta 9601 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg 9661 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct 9721 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta 9781 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg 9841 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga 9901 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt 9961 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca 10021 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt 10081 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt 10141 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga 10201 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt 10261 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc 10321 gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct 10381 atggtcgact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc 10441 tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca 10501 aggcttgacc gacaattgca tgaagaatct gcttagg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.