pLV3-EF1a-SLC25A26-3×FLAG-PGK-

pLV3-EF1a-SLC25A26-3×FLAG-PGK-CopGFP-Puro plasmid

PVT111272

pLV3-EF1a-SLC25A26-3×FLAG-PGK-CopGFP-Puro plasmid Information

Plasmid name: pLV3-EF1a-SLC25A26(human)-3×FLAG-PGK-CopGFP-Puro
Alias: NM_173471.4;COXPD28; SAMC
Gene lenght: 822
Host: Mammalian cells,lentivirus
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: EF1a
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: 5' LTR (truncated) -F1
Reverse primer:
Copy number: High
Induction:

pLV3-EF1a-SLC25A26-3×FLAG-PGK-CopGFP-Puro plasmid Description

pLV3-EF1a-SLC25A26-3×FLAG-PGK-CopGFP-Puro It is a plasmid suitable for gene expression in mammalian cells, and is also suitable for lentivirus packaging.

pLV3-EF1a-SLC25A26-3×FLAG-PGK-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt 1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc 1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga 1921 tactagtaag gatctgcgat cgctccggtg cccgtcagtg ggcagagcgc acatcgccca 1981 cagtccccga gaagttgggg ggaggggtcg gcaattgaac gggtgcctag agaaggtggc 2041 gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg 2101 gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg 2161 ccagaacaca gctgaagctt cgaggggctc gcatctctcc ttcacgcgcc cgccgcccta 2221 cctgaggccg ccatccacgc cggttgagtc gcgttctgcc gcctcccgcc tgtggtgcct 2281 cctgaactgc gtccgccgtc taggtaagtt taaagctcag gtcgagaccg ggcctttgtc 2341 cggcgctccc ttggagccta cctagactca gccggctctc cacgctttgc ctgaccctgc 2401 ttgctcaact ctacgtcttt gtttcgtttt ctgttctgcg ccgttacaga tccaagctgt 2461 gaccggcgcc tactctagag ctagcgaatt cGCCACCatg gaccggccgg ggttcgtggc 2521 agcgctggtg gctggtgggg tagcaggtgt ttctgttgac ttgatattat ttcctctgga 2581 taccattaaa accaggctgc agagtcccca aggatttagt aaggctggtg gttttcatgg 2641 aatatatgct ggcgttcctt ctgctgctat tggatccttt cctaatgctg ctgcattttt 2701 tatcacctat gaatatgtga agtggttttt gcatgctgat tcatcttcat atttgacacc 2761 tatgaaacat atgttggctg cctctgctgg agaagtggtt gcctgcctga ttcgagttcc 2821 atctgaagtg gttaagcaga gggcacaggt atctgcttct acaagaacat ttcagatttt 2881 ctctaacatc ttatatgaag agggtatcca agggttgtat cgaggctata aaagcacagt 2941 tttaagagag attccttttt ctttggtcca gtttccctta tgggagtcct taaaagccct 3001 ctggtcctgg aggcaggatc atgtggtgga ttcttggcag tcagcagtct gtggagcttt 3061 tgcaggtgga tttgccgctg cagtcaccac ccctctagac gtggcaaaga caagaattac 3121 gctggcaaag gctggctcca gcactgctga tgggaatgtg ctctctgtcc tgcatggggt 3181 ctggcggtca caggggctgg caggattatt tgcaggtgtc ttccctcgaa tggcagccat 3241 cagtctggga ggtttcatct ttctgggggc ttatgaccga acgcacagct tgctgttgga 3301 agttggcaga aagagtcctg gatccGACTA CAAAGACCAT GACGGTGATT ATAAAGATCA 3361 TGACATCGAC TACAAGGATG ACGATGACAA Gtagcggccg ctgggtaggg gaggcgcttt 3421 tcccaaggca gtctggagca tgcgctttag cagccccgct gggcacttgg cgctacacaa 3481 gtggcctctg gcctcgcaca cattccacat ccaccggtag gcgccaaccg gctccgttct 3541 ttggtggccc cttcgcgcca ccttctactc ctcccctagt caggaagttc ccccccgccc 3601 cgcagctcgc gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact agtctcgtgc 3661 agatggacag caccgctgag caatggaagc gggtaggcct ttggggcagc ggccaatagc 3721 agctttgctc cttcgctttc tgggctcaga ggctgggaag gggtgggtcc gggggcgggc 3781 tcaggggcgg gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc ccggcattct 3841 gcacgcttca aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct ccgggccttt 3901 cgacctgcag cccaagctta ccatggagag cgacgagagc ggcctgcccg ccatggagat 3961 cgagtgccgc atcaccggca ccctgaacgg cgtggagttc gagctggtgg gcggcggaga 4021 gggcaccccc aagcagggcc gcatgaccaa caagatgaag agcaccaaag gcgccctgac 4081 cttcagcccc tacctgctga gccacgtgat gggctacggc ttctaccact tcggcaccta 4141 ccccagcggc tacgagaacc ccttcctgca cgccatcaac aacggcggct acaccaacac 4201 ccgcatcgag aagtacgagg acggcggcgt gctgcacgtg agcttcagct accgctacga 4261 ggccggccgc gtgatcggcg acttcaaggt ggtgggcacc ggcttccccg aggacagcgt 4321 gatcttcacc gacaagatca tccgcagcaa cgccaccgtg gagcacctgc accccatggg 4381 cgataacgtg ctggtgggca gcttcgcccg caccttcagc ctgcgcgacg gcggctacta 4441 cagcttcgtg gtggacagcc acatgcactt caagagcgcc atccacccca gcatcctgca 4501 gaacgggggc cccatgttcg ccttccgccg cgtggaggag ctgcacagca acaccgagct 4561 gggcatcgtg gagtaccagc acgccttcaa gacccccatc gccttcgcca gatcccgcgc 4621 tcagtcgtcc aattctgccg tggacggcac cgccggaccc ggctccaccg gatctcgcga 4681 gggcagagga agtcttctaa catgcggtga cgtggaggag aatcccggcc ctatgaccga 4741 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct 4801 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat 4861 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa 4921 ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga 4981 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct 5041 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg 5101 gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt 5161 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc 5221 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga 5281 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gagtcgacaa 5341 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc 5401 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat 5461 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg 5521 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg 5581 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat 5641 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt 5701 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc 5761 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa 5821 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg 5881 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg gtacctttaa 5941 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac 6001 tggaagggct aattcactcc caacgaaaat aagatctgct ttttgcttgt actgggtctc 6061 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 6121 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 6181 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta 6241 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg 6301 agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt 6361 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg 6421 tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccagtt ccgcccattc 6481 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc 6541 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagactttt gcagagacgg 6601 cccaaattcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat 6661 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag 6721 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg 6781 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc 6841 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc 6901 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa 6961 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt 7021 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg 7081 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg 7141 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag 7201 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc 7261 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa 7321 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg 7381 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc 7441 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac 7501 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg 7561 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt 7621 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt 7681 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa 7741 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga 7801 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt 7861 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg 7921 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga 7981 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga 8041 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg 8101 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc 8161 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc 8221 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca 8281 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac 8341 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg 8401 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc 8461 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg 8521 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac 8581 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat 8641 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata 8701 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa 8761 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg 8821 tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat 8881 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg 8941 tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga 9001 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag 9061 aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc 9121 ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt 9181 aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa 9241 gctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.