pAOX1-FCY1-Myc-6×His-Zeo plasm

pAOX1-FCY1-Myc-6×His-Zeo plasmid

PVT111258

pAOX1-FCY1-Myc-6×His-Zeo plasmid Information

Plasmid name: pAOX1-FCY1(yeast)-Myc-6×His-Zeo
Alias:
Gene lenght: 474
Host: Yeast
Use(s): Gene expression
Fragment type: ORF
Species: Yeast
Prokaryotic resistance: Zeo
Screening marker: Zeo
Fluorescent labeling:
Promoter: AOX1
Replicon: pUC
Growth Strain(s): DH10B
Growth Temperature: 37°C
Vector backbone:
Forward primer:
Reverse primer: AOX1 terminator-R1
Copy number: Low
Induction:

pAOX1-FCY1-Myc-6×His-Zeo plasmid Description

pAOX1-FCY1-Myc-6×His-Zeo is a plasmid suitable for gene expression in yeast.

pAOX1-FCY1-Myc-6×His-Zeo plasmid Sequence

1 AGATCTAACA TCCAAAGACG AAAGGTTGAA TGAAACCTTT TTGCCATCCG ACATCCACAG 61 GTCCATTCTC ACACATAAGT GCCAAACGCA ACAGGAGGGG ATACACTAGC AGCAGACCGT 121 TGCAAACGCA GGACCTCCAC TCCTCTTCTC CTCAACACCC ACTTTTGCCA TCGAAAAACC 181 AGCCCAGTTA TTGGGCTTGA TTGGAGCTCG CTCATTCCAA TTCCTTCTAT TAGGCTACTA 241 ACACCATGAC TTTATTAGCC TGTCTATCCT GGCCCCCCTG GCGAGGTTCA TGTTTGTTTA 301 TTTCCGAATG CAACAAGCTC CGCATTACAC CCGAACATCA CTCCAGATGA GGGCTTTCTG 361 AGTGTGGGGT CAAATAGTTT CATGTTCCCC AAATGGCCCA AAACTGACAG TTTAAACGCT 421 GTCTTGGAAC CTAATATGAC AAAAGCGTGA TCTCATCCAA GATGAACTAA GTTTGGTTCG 481 TTGAAATGCT AACGGCCAGT TGGTCAAAAA GAAACTTCCA AAAGTCGGCA TACCGTTTGT 541 CTTGTTTGGT ATTGATTGAC GAATGCTCAA AAATAATCTC ATTAATGCTT AGCGCAGTCT 601 CTCTATCGCT TCTGAACCCC GGTGCACCTG TGCCGAAACG CAAATGGGGA AACACCCGCT 661 TTTTGGATGA TTATGCATTG TCTCCACATT GTATGCTTCC AAGATTCTGG TGGGAATACT 721 GCTGATAGCC TAACGTTCAT GATCAAAATT TAACTGTTCT AACCCCTACT TGACAGCAAT 781 ATATAAACAG AAGGAAGCTG CCCTGTCTTA AACCTTTTTT TTTATCATCA TTATTAGCTT 841 ACTTTCATAA TTGCGACTGG TTCCAATTGA CAAGCTTTTG ATTTTAACGA CTTTTAACGA 901 CAACTTGAGA AGATCAAAAA ACAACTAATT ATTCGAAACG ATGAGATTTC CTTCAATTTT 961 TACTGCTGTT TTATTCGCAG CATCCTCCGC ATTAGCTGCT CCAGTCAACA CTACAACAGA 1021 AGATGAAACG GCACAAATTC CGGCTGAAGC TGTCATCGGT TACTCAGATT TAGAAGGGGA 1081 TTTCGATGTT GCTGTTTTGC CATTTTCCAA CAGCACAAAT AACGGGTTAT TGTTTATAAA 1141 TACTACTATT GCCAGCATTG CTGCTAAAGA AGAAGGGGTA TCTCTCGAGA AAAGAGAGGC 1201 TGAAGCTGAA TTCatggtga cagggggaat ggcaagcaag tgggatcaga agggtatgga 1261 cattgcctat gaggaggcgg ccttaggtta caaagagggt ggtgttccta ttggcggatg 1321 tcttatcaat aacaaagacg gaagtgttct cggtcgtggt cacaacatga gatttcaaaa 1381 gggatccgcc acactacatg gtgagatctc cactttggaa aactgtggga gattagaggg 1441 caaagtgtac aaagatacca ctttgtatac gacgctgtct ccatgcgaca tgtgtacagg 1501 tgccatcatc atgtatggta ttccacgctg tgttgtcggt gagaacgtta atttcaaaag 1561 taagggcgag aaatatttac aaactagagg tcacgaggtt gttgttgttg acgatgagag 1621 gtgtaaaaag atcatgaaac aatttatcga tgaaagacct caggattggt ttgaagatat 1681 tggtgagGGT CTAGAACAAA AACTCATCTC AGAAGAGGAT CTGAATAGCG CCGTCGACCA 1741 TCATCATCAT CATCATTGAG TTTGTAGCCT TAGACATGAC TGTTCCTCAG TTCAAGTTGG 1801 GCACTTACGA GAAGACCGGT CTTGCTAGAT TCTAATCAAG AGGATGTCAG AATGCCATTT 1861 GCCTGAGAGA TGCAGGCTTC ATTTTTGATA CTTTTTTATT TGTAACCTAT ATAGTATAGG 1921 ATTTTTTTTG TCATTTTGTT TCTTCTCGTA CGAGCTTGCT CCTGATCAGC CTATCTCGCA 1981 GCTGATGAAT ATCTTGTGGT AGGGGTTTGG GAAAATCATT CGAGTTTGAT GTTTTTCTTG 2041 GTATTTCCCA CTCCTCTTCA GAGTACAGAA GATTAAGTGA GACCTTCGTT TGTGCGGATC 2101 CCCCACACAC CATAGCTTCA AAATGTTTCT ACTCCTTTTT TACTCTTCCA GATTTTCTCG 2161 GACTCCGCGC ATCGCCGTAC CACTTCAAAA CACCCAAGCA CAGCATACTA AATTTCCCCT 2221 CTTTCTTCCT CTAGGGTGTC GTTAATTACC CGTACTAAAG GTTTGGAAAA GAAAAAAGAG 2281 ACCGCCTCGT TTCTTTTTCT TCGTCGAAAA AGGCAATAAA AATTTTTATC ACGTTTCTTT 2341 TTCTTGAAAT TTTTTTTTTT TGATTTTTTT CTCTTTCGAT GACCTCCCAT TGATATTTAA 2401 GTTAATAAAC GGTCTTCAAT TTCTCAAGTT TCAGTTTCAT TTTTCTTGTT CTATTACAAC 2461 TTTTTTTACT TCTTGTTCAT TAGAAAGAAA GCATAGCAAT CTAATCTAAG GGGCGGTGTT 2521 GACAATTAAT CATCGGCATA GTATATCGGC ATAGTATAAT ACGACAAGGT GAGGAACTAA 2581 ACCATGGCCA AGTTGACCAG TGCCGTTCCG GTGCTCACCG CGCGCGACGT CGCCGGAGCG 2641 GTCGAGTTCT GGACCGACCG GCTCGGGTTC TCCCGGGACT TCGTGGAGGA CGACTTCGCC 2701 GGTGTGGTCC GGGACGACGT GACCCTGTTC ATCAGCGCGG TCCAGGACCA GGTGGTGCCG 2761 GACAACACCC TGGCCTGGGT GTGGGTGCGC GGCCTGGACG AGCTGTACGC CGAGTGGTCG 2821 GAGGTCGTGT CCACGAACTT CCGGGACGCC TCCGGGCCGG CCATGACCGA GATCGGCGAG 2881 CAGCCGTGGG GGCGGGAGTT CGCCCTGCGC GACCCGGCCG GCAACTGCGT GCACTTCGTG 2941 GCCGAGGAGC AGGACTGACA CGTCCGACGG CGGCCCACGG GTCCCAGGCC TCGGAGATCC 3001 GTCCCCCTTT TCCTTTGTCG ATATCATGTA ATTAGTTATG TCACGCTTAC ATTCACGCCC 3061 TCCCCCCACA TCCGCTCTAA CCGAAAAGGA AGGAGTTAGA CAACCTGAAG TCTAGGTCCC 3121 TATTTATTTT TTTATAGTTA TGTTAGTATT AAGAACGTTA TTTATATTTC AAATTTTTCT 3181 TTTTTTTCTG TACAGACGCG TGTACGCATG TAACATTATA CTGAAAACCT TGCTTGAGAA 3241 GGTTTTGGGA CGCTCGAAGG CTTTAATTTG CAAGCTGGAG ACCAACATGT GAGCAAAAGG 3301 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 3361 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 3421 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 3481 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 3541 ATGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 3601 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 3661 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 3721 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 3781 TAGAAGGACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 3841 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 3901 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 3961 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATC

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.