pLV3-CMV-3×FLAG-HA-Kif20a-Neo

pLV3-CMV-3×FLAG-HA-Kif20a-Neo plasmid

PVT111027

pLV3-CMV-3×FLAG-HA-Kif20a-Neo plasmid Information

Plasmid name: pLV3-CMV-3×FLAG-HA-Kif20a(mouse)-Neo
Alias: NM_001166406.1;Rab6kifl
Gene lenght: 2664
Host: Mammalian cells,lentivirus
Use(s): Gene expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-3×FLAG-HA-Kif20a-Neo plasmid Description

pLV3-CMV-3×FLAG-HA-Kif20a-Neo It is a plasmid suitable for gene expression in mammalian cells, and is also suitable for lentivirus packaging.

pLV3-CMV-3×FLAG-HA-Kif20a-Neo plasmid Sequence

1 ACGCGTGTAG TCTTATGCAA TACTCTTGTA GTCTTGCAAC ATGGTAACGA TGAGTTAGCA 61 ACATGCCTTA CAAGGAGAGA AAAAGCACCG TGCATGCCGA TTGGTGGAAG TAAGGTGGTA 121 CGATCGTGCC TTATTAGGAA GGCAACAGAC GGGTCTGACA TGGATTGGAC GAACCACTGA 181 ATTGCCGCAT TGCAGAGATA TTGTATTTAA GTGCCTAGCT CGATACAATA AACGGGTCTC 241 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 301 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 361 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 421 CCCGAACAGG GACCTGAAAG CGAAAGGGAA ACCAGAGCTC TCTCGACGCA GGACTCGGCT 481 TGCTGAAGCG CGCACGGCAA GAGGCGAGGG GCGGCGACTG GTGAGTACGC CAAAAATTTT 541 GACTAGCGGA GGCTAGAAGG AGAGAGATGG GTGCGAGAGC GTCAGTATTA AGCGGGGGAG 601 AATTAGATCG CGATGGGAAA AAATTCGGTT AAGGCCAGGG GGAAAGAAAA AATATAAATT 661 AAAACATATA GTATGGGCAA GCAGGGAGCT AGAACGATTC GCAGTTAATC CTGGCCTGTT 721 AGAAACATCA GAAGGCTGTA GACAAATACT GGGACAGCTA CAACCATCCC TTCAGACAGG 781 ATCAGAAGAA CTTAGATCAT TATATAATAC AGTAGCAACC CTCTATTGTG TGCATCAAAG 841 GATAGAGATA AAAGACACCA AGGAAGCTTT AGACAAGATA GAGGAAGAGC AAAACAAAAG 901 TAAGACCACC GCACAGCAAG CGGCCACTGA TCTTCAGACC TGGAGGAGGA GATATGAGGG 961 ACAATTGGAG AAGTGAATTA TATAAATATA AAGTAGTAAA AATTGAACCA TTAGGAGTAG 1021 CACCCACCAA GGCAAAGAGA AGAGTGGTGC AGAGAGAAAA AAGAGCAGTG GGAATAGGAG 1081 CTTTGTTCCT TGGGTTCTTG GGAGCAGCAG GAAGCACTAT GGGCGCAGCC TCAATGACGC 1141 TGACGGTACA GGCCAGACAA TTATTGTCTG GTATAGTGCA GCAGCAGAAC AATTTGCTGA 1201 GGGCTATTGA GGCGCAACAG CATCTGTTGC AACTCACAGT CTGGGGCATC AAGCAGCTCC 1261 AGGCAAGAAT CCTGGCTGTG GAAAGATACC TAAAGGATCA ACAGCTCCTG GGGATTTGGG 1321 GTTGCTCTGG AAAACTCATT TGCACCACTG CTGTGCCTTG GAATGCTAGT TGGAGTAATA 1381 AATCTCTGGA ACAGATTGGA ATCACACGAC CTGGATGGAG TGGGACAGAG AAATTAACAA 1441 TTACACAAGC TTAATACACT CCTTAATTGA AGAATCGCAA AACCAGCAAG AAAAGAATGA 1501 ACAAGAATTA TTGGAATTAG ATAAATGGGC AAGTTTGTGG AATTGGTTTA ACATAACAAA 1561 TTGGCTGTGG TATATAAAAT TATTCATAAT GATAGTAGGA GGCTTGGTAG GTTTAAGAAT 1621 AGTTTTTGCT GTACTTTCTA TAGTGAATAG AGTTAGGCAG GGATATTCAC CATTATCGTT 1681 TCAGACCCAC CTCCCAACCC CGAGGGGACC CGACAGGCCC GAAGGAATAG AAGAAGAAGG 1741 TGGAGAGAGA GACAGAGACA GATCCATTCG ATTAGTGAAC GGATCTCGAC GGTATCGGTT 1801 AACTTTTAAA AGAAAAGGGG GGATTGGGGG GTACAGTGCA GGGGAAAGAA TAGTAGACAT 1861 AATAGCAACA GACATACAAA CTAAAGAATT ACAAAAACAA ATTACAAAAT TCAAAATTTT 1921 ATCGATACTA GTATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA 1981 TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC 2041 GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA 2101 GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT 2161 TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTTTA TATAAGCAGA GCTCGTTTAG 2221 TGAACCGTCA GATCGCCTGG AGACGCCATC CACGCTGTTT TGACCTCCAT AGAAGATTCT 2281 AGAGCTAGCC ACCATGGACT ACAAAGACCA TGACGGTGAT TATAAAGATC ATGACATCGA 2341 CTACAAGGAT GACGATGACA AGGAATTCTA CCCATACGAT GTTCCAGATT ACGCTatgtc 2401 tcaccggatc ctttctccgc cagcgggctt actctctgat gaggatgttg tagactctcc 2461 catattagaa tccacagctg cagatctgag atctgttgtc cgaaaggacc tgttgtcaga 2521 ctgctctgtc atctctgcct ctctggagga caagcaggct ctactggagg acacctcaga 2581 gaaggtgaaa gtttaccttc gaattcgacc attcttgact tcagagttgg accgacagga 2641 agatcagggc tgcgtctgca ttgagaatac agaaaccctt gtgctgcagg cacccaaaga 2701 ctcctttgcc ttgaagagta atgagagggg agttggtcag gccactcaca aatttacctt 2761 ttcccagata tttgggccag aagtgggaca ggtggccttc ttcaatctga ccatgaagga 2821 gatggtgaag gatgtgctca aagggcagaa ctggctcatc tacacctatg gagtcaccaa 2881 ctcggggaaa acgtacacca ttcaaggtac tagtaaggat gcggggatcc tgccccagtc 2941 cctggctctc atctttaata gtctccaggg ccagcttcat ccgacacctg acctgaagcc 3001 cttattgtcc aatgaagtaa tctggctaga cagcaagcag attcgacagg aggaaatgaa 3061 gaagctgtcc ttgctaattg gaggcctcca agaggaggag ctgtccacct ccgtgaagaa 3121 acgtgtccac actgaaagtc ggataggtgc cagcaacagc tttgacagtg gcgttgctgg 3181 gctgtcttcc accagtcagt tcaccagcag cagccagcta gatgaaacaa gtcaattatg 3241 ggcacaacca gacactgtcc cagtaagtgt tccagcagac attcgcttct ctgtctggat 3301 ctccttcttt gagatctaca atgaattgct ttatgacctg ttagaaccac ctagccatca 3361 gcataagaga cagacacttc ggctgtgtga ggatcagaac ggcaatcctt acgtgaaaga 3421 tctcaattgg attcatgttc gtgatgttga ggaagcctgg aaactattga aagtgggtcg 3481 caagaaccag agctttgcca gcacccacat gaaccagcaa tccagccgca gtcacagcat 3541 cttctcaatc agaatcttgc accttcaggg agaaggggat atagtgccca agatcagtga 3601 actgtcactc tgtgacctgg ctggctctga gcgctgcaaa catcaaaaaa gtggtgagcg 3661 gctaaaggag gcagggaaca ttaacacttc tctgcacacc ctgggccgct gtattgctgc 3721 cctgcgacag aatcagcaga accggtcaaa gcagaacctg attcctttcc gtgacagcaa 3781 gttgactcgt gtgttccaag gcttcttcac aggtcgaggt cgttcctgta tgattgtcaa 3841 tgtgaatccc tgtgcatcta catatgacga gactcttcat gcagccaaat tctcagccct 3901 agccagccag cttgtgcacg ccccacctgt gcatctggga atcccatccc tgcattcatt 3961 catcaagaag cacagtcctc aggttggccc tggcttagag aaagaggaca aggccgactc 4021 agaccttgag gacagtcctg aagacgaagc tgacgtctcc gtgtatggca aggaggaact 4081 actccaggtg gtggaagcca tgaaagcgct acttttaaaa gaacgacaag aaaagctgca 4141 gttggagata cagctccgag aggaaatttg caacgaaatg gtggaacaga tgcaacagcg 4201 ggagcagtgg tgcagtgagc gtttggacaa ccaaaaggaa ctgatggagg aactgtacga 4261 ggagaaactg aagatcctga aggaatcact gacaactttc taccaagagc agatccagga 4321 gcgggatgaa aaaattgaag agctagaaac tctgttgcag gaagccaaac agcagccagc 4381 ggcacaacag tcaggggggc tgtccctcct ccggcggtca cagaggctgg cagcgtctgc 4441 ctccactcag cagttccagg aagttaaagc tgaactagag caatgcaaaa cagagttaag 4501 ctctaccact gcagagctgc acaagtatca gcaagtgtta aaaccgccac ccccagccaa 4561 gcctttcacc attgatgtgg ataagaagtt agaggagggc cagaagaata taagactgct 4621 acggacagag ctccagaaac taggacagtc tctgcagtca gcagaaagag cctgttgcca 4681 cagcaccgga gcaggaaaac ttcgtcaagc attaaccaac tgtgatgaca tcttaatcaa 4741 gcagaaccag accctggctg agctgcagaa taacatggtg ctagtgaaac tggaccttca 4801 gaaaaaggca gcctgcattg ctgagcagta tcacactgtg ctaaagctcc aaggccaagc 4861 ctctgccaaa aagcggctgg gagcaaacca ggaaaaccag caaccaaatc atcaaccccc 4921 aggaaagaaa ccattcctgc gaaacttact tccccgaaca cctacctgcc aaagctcaac 4981 agacagcagc ccttatgcgc ggatcttgcg ctcacggcac tctcctttac tcaaatctcc 5041 ctttggcaaa aaatactaaG CGGCCGCGAA GGATCTGCGA TCGCTCCGGT GCCCGTCAGT 5101 GGGCAGAGCG CACATCGCCC ACAGTCCCCG AGAAGTTGGG GGGAGGGGTC GGCAATTGAA 5161 CGGGTGCCTA GAGAAGGTGG CGCGGGGTAA ACTGGGAAAG TGATGTCGTG TACTGGCTCC 5221 GCCTTTTTCC CGAGGGTGGG GGAGAACCGT ATATAAGTGC AGTAGTCGCC GTGAACGTTC 5281 TTTTTCGCAA CGGGTTTGCC GCCAGAACAC AGCTGAAGCT TCGAGGGGCT CGCATCTCTC 5341 CTTCACGCGC CCGCCGCCCT ACCTGAGGCC GCCATCCACG CCGGTTGAGT CGCGTTCTGC 5401 CGCCTCCCGC CTGTGGTGCC TCCTGAACTG CGTCCGCCGT CTAGGTAAGT TTAAAGCTCA 5461 GGTCGAGACC GGGCCTTTGT CCGGCGCTCC CTTGGAGCCT ACCTAGACTC AGCCGGCTCT 5521 CCACGCTTTG CCTGACCCTG CTTGCTCAAC TCTACGTCTT TGTTTCGTTT TCTGTTCTGC 5581 GCCGTTACAG ATCCAAGCTG TGACCGGCGC CTACGCTAGG CCACCatggc tgaacaagat 5641 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca 5701 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg 5761 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg 5821 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact 5881 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct 5941 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg 6001 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt 6061 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc 6121 gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc 6181 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga 6241 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc 6301 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt 6361 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga 6421 GTCGACAATC AACCTCTGGA TTACAAAATT TGTGAAAGAT TGACTGGTAT TCTTAACTAT 6481 GTTGCTCCTT TTACGCTATG TGGATACGCT GCTTTAATGC CTTTGTATCA TGCTATTGCT 6541 TCCCGTATGG CTTTCATTTT CTCCTCCTTG TATAAATCCT GGTTGCTGTC TCTTTATGAG 6601 GAGTTGTGGC CCGTTGTCAG GCAACGTGGC GTGGTGTGCA CTGTGTTTGC TGACGCAACC 6661 CCCACTGGTT GGGGCATTGC CACCACCTGT CAGCTCCTTT CCGGGACTTT CGCTTTCCCC 6721 CTCCCTATTG CCACGGCGGA ACTCATCGCC GCCTGCCTTG CCCGCTGCTG GACAGGGGCT 6781 CGGCTGTTGG GCACTGACAA TTCCGTGGTG TTGTCGGGGA AATCATCGTC CTTTCCTTGG 6841 CTGCTCGCCT GTGTTGCCAC CTGGATTCTG CGCGGGACGT CCTTCTGCTA CGTCCCTTCG 6901 GCCCTCAATC CAGCGGACCT TCCTTCCCGC GGCCTGCTGC CGGCTCTGCG GCCTCTTCCG 6961 CGTCTTCGCC TTCGCCCTCA GACGAGTCGG ATCTCCCTTT GGGCCGCCTC CCCGCCTGGT 7021 ACCTTTAAGA CCAATGACTT ACAAGGCAGC TGTAGATCTT AGCCACTTTT TAAAAGAAAA 7081 GGGGGGACTG GAAGGGCTAA TTCACTCCCA ACGAAAATAA GATCTGCTTT TTGCTTGTAC 7141 TGGGTCTCTC TGGTTAGACC AGATCTGAGC CTGGGAGCTC TCTGGCTAAC TAGGGAACCC 7201 ACTGCTTAAG CCTCAATAAA GCTTGCCTTG AGTGCTTCAA GTAGTGTGTG CCCGTCTGTT 7261 GTGTGACTCT GGTAACTAGA GATCCCTCAG ACCCTTTTAG TCAGTGTGGA AAATCTCTAG 7321 CAGTAGTAGT TCATGTCATC TTATTATTCA GTATTTATAA CTTGCAAAGA AATGAATATC 7381 AGAGAGTGAG AGGAACTTGT TTATTGCAGC TTATAATGGT TACAAATAAA GCAATAGCAT 7441 CACAAATTTC ACAAATAAAG CATTTTTTTC ACTGCATTCT AGTTGTGGTT TGTCCAAACT 7501 CATCAATGTA TCTTATCATG TCTGGCTCTA GCTATCCCGC CCCTAACTCC GCCCAGTTCC 7561 GCCCATTCTC CGCCCCATGG CTGACTAATT TTTTTTATTT ATGCAGAGGC CGAGGCCGCC 7621 TCGGCCTCTG AGCTATTCCA GAAGTAGTGA GGAGGCTTTT TTGGAGGCCT AGACTTTTGC 7681 AGAGACGGCC CAAATTCGTA ATCATGGTCA TAGCTGTTTC CTGTGTGAAA TTGTTATCCG 7741 CTCACAATTC CACACAACAT ACGAGCCGGA AGCATAAAGT GTAAAGCCTG GGGTGCCTAA 7801 TGAGTGAGCT AACTCACATT AATTGCGTTG CGCTCACTGC CCGCTTTCCA GTCGGGAAAC 7861 CTGTCGTGCC AGCTGCATTA ATGAATCGGC CAACGCGCGG GGAGAGGCGG TTTGCGTATT 7921 GGGCGCTCTT CCGCTTCCTC GCTCACTGAC TCGCTGCGCT CGGTCGTTCG GCTGCGGCGA 7981 GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA 8041 GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG 8101 CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT 8161 CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC 8221 CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT 8281 TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC 8341 GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA 8401 TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA 8461 GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG 8521 TGGTGGCCTA ACTACGGCTA CACTAGAAGG ACAGTATTTG GTATCTGCGC TCTGCTGAAG 8581 CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT 8641 AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA 8701 GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG 8761 ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA 8821 AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA 8881 ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC 8941 CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG 9001 ATACCGCGAG ACCCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA 9061 AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT 9121 TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT 9181 GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC 9241 CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC 9301 GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA 9361 GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG 9421 TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG 9481 TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA 9541 CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA 9601 CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA 9661 GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA 9721 ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 9781 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 9841 CCCCGAAAAG TGCCACCTGA CGTCTAAGAA ACCATTATTA TCATGACATT AACCTATAAA 9901 AATAGGCGTA TCACGAGGCC CTTTCGTCTC GCGCGTTTCG GTGATGACGG TGAAAACCTC 9961 TGACACATGC AGCTCCCGGA GACGGTCACA GCTTGTCTGT AAGCGGATGC CGGGAGCAGA 10021 CAAGCCCGTC AGGGCGCGTC AGCGGGTGTT GGCGGGTGTC GGGGCTGGCT TAACTATGCG 10081 GCATCAGAGC AGATTGTACT GAGAGTGCAC CATATGCGGT GTGAAATACC GCACAGATGC 10141 GTAAGGAGAA AATACCGCAT CAGGCGCCAT TCGCCATTCA GGCTGCGCAA CTGTTGGGAA 10201 GGGCGATCGG TGCGGGCCTC TTCGCTATTA CGCCAGCTGG CGAAAGGGGG ATGTGCTGCA 10261 AGGCGATTAA GTTGGGTAAC GCCAGGGTTT TCCCAGTCAC GACGTTGTAA AACGACGGCC 10321 AGTGCCAAGC TG

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.