pCAGGS-FLAG-RNF40 plasmid

pCAGGS-FLAG-RNF40 plasmid

PVT110338

pCAGGS-FLAG-RNF40 plasmid Information

Plasmid name: pCAGGS-FLAG-RNF40(human)
Alias: NM_014771.4;BRE1B; RBP95; STARING
Gene lenght: 3006
Host: Mammalian cells
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling:
Promoter: CAG
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: pcaggs-F
Reverse primer:
Copy number: High
Induction:

pCAGGS-FLAG-RNF40 plasmid Description

pCAGGS-FLAG-RNF40 It is a plasmid suitable for gene expression in mammalian cells.

pCAGGS-FLAG-RNF40 plasmid Sequence

1 GTCGACATTG ATTATTGACT AGTTATTAAT AGTAATCAAT TACGGGGTCA TTAGTTCATA 61 GCCCATATAT GGAGTTCCGC GTTACATAAC TTACGGTAAA TGGCCCGCCT GGCTGACCGC 121 CCAACGACCC CCGCCCATTG ACGTCAATAA TGACGTATGT TCCCATAGTA ACGCCAATAG 181 GGACTTTCCA TTGACGTCAA TGGGTGGAGT ATTTACGGTA AACTGCCCAC TTGGCAGTAC 241 ATCAAGTGTA TCATATGCCA AGTACGCCCC CTATTGACGT CAATGACGGT AAATGGCCCG 301 CCTGGCATTA TGCCCAGTAC ATGACCTTAT GGGACTTTCC TACTTGGCAG TACATCTACG 361 TATTAGTCAT CGCTATTACC ATGGTCGAGG TGAGCCCCAC GTTCTGCTTC ACTCTCCCCA 421 TCTCCCCCCC CTCCCCACCC CCAATTTTGT ATTTATTTAT TTTTTAATTA TTTTGTGCAG 481 CGATGGGGGC GGGGGGGGGG GGGGGGCCCC CCCCAGGCGG GGCGGGGCGG GGCGAGGGGC 541 GGGGCGGGGC GAGGCGGAAA GGTGCGGCGG CAGCCAATCA GAGCGGCGCG CTCCGAAAGT 601 TTCCTTTTAT GGCGAGGCGG CGGCGGCGGC GGCCCTATAA AAAGCGAAGC GCGCGGCGGG 661 CGGGAGTCGT TGCGCGCTGC CTTCCCCCCG TGCCCCGCTC CGCCGCCGCC TCGCGCCGCC 721 CGCCCCGGCT CTGACTGACC GCGTTACTCC CACAGGTGAG CGGGCGGGAC GGCCCTTCTC 781 CTCCGGGCTG TAATTAGCGC TTGGTTTAAT GACGGCTTGT TTCTTTTCTG TGGCTGCGTG 841 AAAGCCTTGA GGGGCTCCGG GAGGGCCCTT TGTGCGGGGG GAGCGGCTCG GGGGGTGCGT 901 GCGTGTGTGT GTGCGTGGGG AGCGCCGCGT GCGGCTCCGC GCTGCCCGGC GGCTGTGAGC 961 GCTGCGGGCG CGGCGCGGGG CTTTGTGCGC TCCGCAGTGT GCGCGAGGGG AGCGCGGCCG 1021 GGGGCGGTGC CCCGCGGTGC GGGGGGGGCT GCGAGGGGAA CAAAGGCTGC GTGCGGGGTG 1081 TGTGCGTGGG GGGGTGAGCA GGGGGTGTGG GCGCGTCGGT CGGGCTGCAA CCCCCCCTGC 1141 ACCCCCCTCC CCGAGTTGCT GAGCACGGCC CGGCTTCGGG TGCGGGGCTC CGTACGGGGC 1201 GTGGCGCGGG GCTCGCCGTG CCGGGCGGGG GGTGGCGGCA GGTGGGGGTG CCGGGCGGGG 1261 CGGGGCCGCC TCGGGCCGGG GAGGGCTCGG GGGAAGGGGC GCGGCGGCCC CCGGAGCGCC 1321 GGCGGCTGTC GAGGCGCGGC GAGCCGCAGC CATTGCCTTT TATGGTAATC GTGCGAGAGG 1381 GCGCAGGGAC TTCCTTTGTC CCAAATCTGT GCGGAGCCGA AATCTGGGAG GCGCCGCCGC 1441 ACCCCCTCTA GCGGGCGCGG GGCGAAGCGG TGCGGCGCCG GCAGGAAGGA AATGGGCGGG 1501 GAGGGCCTTC GTGCGTCGCC GCGCCGCCGT CCCCTTCTCC CTCTCCAGCC TCGGGGCTGT 1561 CCGCGGGGGG ACGGCTGCCT TCGGGGGGGA CGGGGCAGGG CGGGGTTCGG CTTCTGGCGT 1621 GTGACCGGCG GCTCTAGAGC CTCTGCTAAC CATGTTCATG CCTTCTTCTT TTTCCTACAG 1681 CTCCTGGGCA ACGTGCTGGT TGTTGTGCTG TCTCATCATT TTGGCAAAGC CACCATGGAT 1741 TACAAGGATG ACGACGATAA GGAATTCatg tctgggccag gcaacaaacg cgccgccggc 1801 gacgggggct cagggccccc ggaaaagaag ctgagtcgtg aggagaagac caccacgact 1861 cttatcgagc ccattcgtct tggaggcatc tcttccacgg aggagatgga cctgaaggta 1921 ctacagttca agaacaagaa actggcagag cggctggaac aacggcaggc ttgtgaagat 1981 gaactccgag aacgaattga gaagttggag aagcggcagg ccacagatga tgccacactc 2041 ctcatcgtca atcgctactg ggcccagctg gatgaaactg tggaagccct tctccgatgc 2101 catgagagcc agggggagct gtcttcagcg cctgaggcac ctgggaccca ggaggggcca 2161 acatgtgatg ggactcctct cccagagccg gggacatcag agctgagaga ccccttgctg 2221 atgcagctgc ggccccctct cagtgagccg gccttggctt ttgtggtggc actgggtgcc 2281 agcagcagtg aggaggtgga gctggagctg caaggccgaa tggagttctc caaggcagct 2341 gtgtctcgtg tggtagaggc ctcagaccgc ctacagcgcc gggtggagga actctgtcag 2401 cgagtgtaca gccgagggga cagtgagccc ctcagtgagg cggctcaggc acacacccga 2461 gagctgggcc gtgagaaccg gcgactgcag gacttggcca ctcagctgca ggagaaacac 2521 caccgcatct cattggagta ctccgagctc caggataaag tgacatcggc agagaccaag 2581 gtgctggaga tggagacaac agtggaggac ttgcagtggg acatcgagaa gctgcggaag 2641 cgagagcaaa agctcaataa gcacctggca gaggccttag agcagcttaa ctctggctac 2701 tatgtatctg ggagctcctc aggcttccag gggggccaga tcacactcag catgcagaag 2761 tttgagatgc tgaatgcaga gttagaggaa aaccaggaac tggccaacag ccgtatggca 2821 gagctggaga aactgcaggc cgaacttcag ggggctgtgc ggaccaatga gcgcctcaag 2881 gtggccctgc ggagccttcc tgaggaggta gtgcgggaga cgggggagta ccgcatgctg 2941 caggcccaat tctcactgct ctacaacgag tctctgcaag tgaagaccca gctagacgag 3001 gctcggggcc tgctgctggc cacaaagaac tcccacctgc gacacatcga gcacatggag 3061 agcgacgagc tggggctgca gaagaagcta cgcacagagg tcattcagct ggaggacacg 3121 ctggcccagg tacgcaagga gtatgagatg ctgcgcatcg agtttgagca gaatctggcg 3181 gccaacgagc aggcggggcc catcaaccgt gagatgcgcc acctgattag tagtcttcaa 3241 aaccacaacc accagctaaa aggggacgcc cagcgataca agcggaagct tcgagaagta 3301 caagctgaga ttggcaagct ccgggcccag gccagtggct ctgcccactc cacccccaac 3361 ctgggccacc cagaggattc tggcgtcagt gccccagccc cagggaaaga ggagggtggg 3421 ccaggccctg tcagtacccc cgacaacaga aaggagatgg ctccagtgcc tggcaccacc 3481 actactacca cttcagtgaa gaaggaggag ctggtcccct ctgaagagga cttccagggt 3541 ataacccctg gggcccaggg cccttcctcc cggggccgag aacctgaggc caggcccaag 3601 cgggagcttc gggaacggga aggtcccagc ctaggacctc cacctgtagc ctccgctctc 3661 tcaagggctg atcgggagaa ggccaaggtg gaagaaacca agcggaagga atcagaactc 3721 ctcaagggtc tccgagcaga gctcaagaag gcccaggaga gccagaagga gatgaaactg 3781 ctgctggata tgtacaagtc agcgcccaag gagcagcggg ataaggtgca gctcatggca 3841 gcggaacgca aggctaaggc cgaggttgat gagctgcgga gccgcatccg ggaattggag 3901 gagagggatc gaagggagag caagaagatc gcggatgagg atgccctgcg gcgcattcgg 3961 caggcagagg agcagataga acacctgcag cgcaagctgg gtgccaccaa gcaggaggag 4021 gaggctctgc tctcagagat ggatgtgaca ggtcaggctt ttgaggacat gcaggaacag 4081 aacgggcggc tgctacagca gttgcgggaa aaggatgatg ccaactttaa gctaatgtca 4141 gagcggatca aggccaacca gattcacaag ctgctgcggg aggagaagga tgagttgggc 4201 gagcaggtcc ttggcctcaa gtcccaggtg gatgcccagc tgctgactgt gcagaagcta 4261 gaggagaagg agcgagcctt gcagggcagc ctcgggggtg tggagaagga gctgacgctg 4321 cgcagccaag ccctggagct caacaagcgg aaggctgtag aagccgccca gctggccgag 4381 gacctgaagg tgcagctgga gcacgtgcag actcggctgc gggagatcca gccctgcctg 4441 gcagagagcc gggctgctcg tgagaaagag agcttcaacc tcaagagggc tcaggaggac 4501 atctcacggc tgcggcgcaa gctggaaaag cagaggaagg tggaggtcta cgcagatgcc 4561 gacgaaatcc tccaggagga gatcaaggag tacaaggcgc ggttgacctg cccctgctgt 4621 aacacccgca agaaggatgc agtccttacc aagtgcttcc acgttttctg cttcgagtgc 4681 gtgcggggcc gctatgaggc ccgccagagg aagtgcccca agtgcaacgc ggcctttggt 4741 gcccacgact tccatcgtat ctacatcagc tgaCTCGAGC TAGCAGATCT TAATTAATTA 4801 AGGATCTTTT TCCCTCTGCC AAAAATTATG GGGACATCAT GAAGCCCCTT GAGCATCTGA 4861 CTTCTGGCTA ATAAAGGAAA TTTATTTTCA TTGCAATAGT GTGTTGGAAT TTTTTGTGTC 4921 TCTCACTCGG AAGGACATAT GGGAGGGCAA ATCATTTAAA ACATCAGAAT GAGTATTTGG 4981 TTTAGAGTTT GGCAACATAT GCCCATATGC TGGCTGCCAT GAACAAAGGT TGGCTATAAA 5041 GAGGTCATCA GTATATGAAA CAGCCCCCTG CTGTCCATTC CTTATTCCAT AGAAAAGCCT 5101 TGACTTGAGG TTAGATTTTT TTTATATTTT GTTTTGTGTT ATTTTTTTCT TTAACATCCC 5161 TAAAATTTTC CTTACATGTT TTACTAGCCA GATTTTTCCT CCTCTCCTGA CTACTCCCAG 5221 TCATAGCTGT CCCTCTTCTC TTATGGAGAT CCCTCGACCT GCAGCCCAAG CTTGGCGTAA 5281 TCATGGTCAT AGCTGTTTCC TGTGTGAAAT TGTTATCCGC TCACAATTCC ACACAACATA 5341 CGAGCCGGAA GCATAAAGTG TAAAGCCTGG GGTGCCTAAT GAGTGAGCTA ACTCACATTA 5401 ATTGCGTTGC GCTCACTGCC CGCTTTCCAG TCGGGAAACC TGTCGTGCCA GCGGATCCGC 5461 ATCTCAATTA GTCAGCAACC ATAGTCCCGC CCCTAACTCC GCCCATCCCG CCCCTAACTC 5521 CGCCCAGTTC CGCCCATTCT CCGCCCCATG GCTGACTAAT TTTTTTTATT TATGCAGAGG 5581 CCGAGGCCGC CTCGGCCTCT GAGCTATTCC AGAAGTAGTG AGGAGGCTTT TTTGGAGGCC 5641 TAGGCTTTTG CAAAAAGCTA ACTTGTTTAT TGCAGCTTAT AATGGTTACA AATAAAGCAA 5701 TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG CATTCTAGTT GTGGTTTGTC 5761 CAAACTCATC AATGTATCTT ATCATGTCTG GATCCGCTGC ATTAATGAAT CGGCCAACGC 5821 GCGGGGAGAG GCGGTTTGCG TATTGGGCGC TCTTCCGCTT CCTCGCTCAC TGACTCGCTG 5881 CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA 5941 TCCACAGAAT CAGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA 6001 GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC 6061 ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC 6121 AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG 6181 GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA 6241 GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG 6301 TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC 6361 ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG 6421 GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT 6481 TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT 6541 CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC 6601 GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT 6661 GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT 6721 AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT 6781 GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC 6841 GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC 6901 CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGACCCACG CTCACCGGCT CCAGATTTAT 6961 CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG 7021 CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA 7081 GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA 7141 TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT 7201 GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG 7261 TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA 7321 GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC 7381 GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT 7441 TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC 7501 TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA 7561 CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA 7621 TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA 7681 TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC 7741 AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.