pLV3-CMV-UBE3A-2-3×FLAG-CopGFP

pLV3-CMV-UBE3A-2-3×FLAG-CopGFP-Puro plasmid

PVT112139

pLV3-CMV-UBE3A-2-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-UBE3A(human)-2-3×FLAG-CopGFP-Puro
Alias: NM_001354505.1;ANCR; AS; E6-AP; EPVE6AP; HPVE6A; PIX1
Gene lenght: 2614
Host: Mammalian cells,lentivirus
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-UBE3A-2-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-UBE3A-2-3×FLAG-CopGFP-Puro is a plasmid suitable for gene expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-UBE3A-2-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgg ccacagcttg taaaagatca 2341 ggagaacctc agtctgacga cattgaagct agccgaatga agcgagcagc tgcaaagcat 2401 ctaatagaac gctactacca ccagttaact gagggctgtg gaaatgaagc ctgcacgaat 2461 gagttttgtg cttcctgtcc aacttttctt cgtatggata ataatgcagc agctattaaa 2521 gccctcgagc tttataagat taatgcaaaa ctctgtgatc ctcatccctc caagaaagga 2581 gcaagctcag cttaccttga gaactcgaaa ggtgccccca acaactcctg ctctgagata 2641 aaaatgaaca agaaaggcgc tagaattgat tttaaagatg tgacttactt aacagaagag 2701 aaggtatatg aaattcttga attatgtaga gaaagagagg attattcccc tttaatccgt 2761 gttattggaa gagttttttc tagtgctgag gcattggtac agagcttccg gaaagttaaa 2821 caacacacca aggaagaact gaaatctctt caagcaaaag atgaagacaa agatgaagat 2881 gaaaaggaaa aagctgcatg ttctgctgct gctatggaag aagactcaga agcatcttcc 2941 tcaaggatag gtgatagctc acagggagac aacaatttgc aaaaattagg ccctgatgat 3001 gtgtctgtgg atattgatgc cattagaagg gtctacacca gattgctctc taatgaaaaa 3061 attgaaactg cctttctcaa tgcacttgta tatttgtcac ctaacgtgga atgtgacttg 3121 acgtatcaca atgtatactc tcgagatcct aattatctga atttgttcat tatcgtaatg 3181 gagaatagaa atctccacag tcctgaatat ctggaaatgg ctttgccatt attttgcaaa 3241 gcgatgagca agctacccct tgcagcccaa ggaaaactga tcagactgtg gtctaaatac 3301 aatgcagacc agattcggag aatgatggag acatttcagc aacttattac ttataaagtc 3361 ataagcaatg aatttaacag tcgaaatcta gtgaatgatg atgatgccat tgttgctgct 3421 tcgaagtgct tgaaaatggt ttactatgca aatgtagtgg gaggggaagt ggacacaaat 3481 cacaatgaag aagatgatga agagcccatc cctgagtcca gcgagctgac acttcaggaa 3541 cttttgggag aagaaagaag aaacaagaaa ggtcctcgag tggaccccct ggaaactgaa 3601 cttggtgtta aaaccctgga ttgtcgaaaa ccacttatcc cttttgaaga gtttattaat 3661 gaaccactga atgaggttct agaaatggat aaagattata cttttttcaa agtagaaaca 3721 gagaacaaat tctcttttat gacatgtccc tttatattga atgctgtcac aaagaatttg 3781 ggattatatt atgacaatag aattcgcatg tacagtgaac gaagaatcac tgttctctac 3841 agcttagttc aaggacagca gttgaatcca tatttgagac tcaaagttag acgtgaccat 3901 atcatagatg atgcacttgt ccggctagag atgatcgcta tggaaaatcc tgcagacttg 3961 aagaagcagt tgtatgtgga atttgaagga gaacaaggag ttgatgaggg aggtgtttcc 4021 aaagaatttt ttcagctggt tgtggaggaa atcttcaatc cagatattgg tatgttcaca 4081 tacgatgaat ctacaaaatt gttttggttt aatccatctt cttttgaaac tgagggtcag 4141 tttactctga ttggcatagt actgggtctg gctatttaca ataactgtat actggatgta 4201 cattttccca tggttgtcta caggaagcta atggggaaaa aaggaacttt tcgtgacttg 4261 ggagactctc acccagttct atatcagagt ttaaaagatt tattggagta tgaagggaat 4321 gtggaagatg acatgatgat cactttccag atatcacaga cagatctttt tggtaaccca 4381 atgatgtatg atctaaagga aaatggtgat aaaattccaa ttacaaatga aaacaggaag 4441 gaatttgtca atctttattc tgactacatt ctcaataaat cagtagaaaa acagttcaag 4501 gcttttcgga gaggttttca tatggtgacc aatgaatctc ccttaaagta cttattcaga 4561 ccagaagaaa ttgaattgct tatatgtgga agccggaatc tagatttcca agcactagaa 4621 gaaactacag aatatgacgg tggctatacc agggactctg ttctgattag ggagttctgg 4681 gaaatcgttc attcatttac agatgaacag aaaagactct tcttgcagtt tacaacgggc 4741 acagacagag cacctgtggg aggactagga aaattaaaga tgattatagc caaaaatggc 4801 ccagacacag aaaggttacc tacatctcat acttgcttta atgtgctttt acttccggaa 4861 tactcaagca aagaaaaact taaagagaga ttgttgaagg ccatcacgta tgccaaagga 4921 tttggcatgc tgggatccGA CTACAAAGAC CATGACGGTG ATTATAAAGA TCATGACATC 4981 GACTACAAGG ATGACGATGA CAAGtagcgg ccgcGaagga tctgcgatcg ctccggtgcc 5041 cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc 5101 aattgaacgg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac 5161 tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg 5221 aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg aggggctcgc 5281 atctctcctt cacgcgcccg ccgccctacc tgaggccgcc atccacgccg gttgagtcgc 5341 gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta ggtaagttta 5401 aagctcaggt cgagaccggg cctttgtccg gcgctccctt ggagcctacc tagactcagc 5461 cggctctcca cgctttgcct gaccctgctt gctcaactct acgtctttgt ttcgttttct 5521 gttctgcgcc gttacagatc caagctgtga ccggcgccta cgctagacgc caccatggag 5581 agcgacgaga gcggcctgcc cgccatggag atcgagtgcc gcatcaccgg caccctgaac 5641 ggcgtggagt tcgagctggt gggcggcgga gagggcaccc ccaagcaggg ccgcatgacc 5701 aacaagatga agagcaccaa aggcgccctg accttcagcc cctacctgct gagccacgtg 5761 atgggctacg gcttctacca cttcggcacc taccccagcg gctacgagaa ccccttcctg 5821 cacgccatca acaacggcgg ctacaccaac acccgcatcg agaagtacga ggacggcggc 5881 gtgctgcacg tgagcttcag ctaccgctac gaggccggcc gcgtgatcgg cgacttcaag 5941 gtggtgggca ccggcttccc cgaggacagc gtgatcttca ccgacaagat catccgcagc 6001 aacgccaccg tggagcacct gcaccccatg ggcgataacg tgctggtggg cagcttcgcc 6061 cgcaccttca gcctgcgcga cggcggctac tacagcttcg tggtggacag ccacatgcac 6121 ttcaagagcg ccatccaccc cagcatcctg cagaacgggg gccccatgtt cgccttccgc 6181 cgcgtggagg agctgcacag caacaccgag ctgggcatcg tggagtacca gcacgccttc 6241 aagaccccca tcgccttcgc cagatcccgc gctcagtcgt ccaattctgc cgtggacggc 6301 accgccggac ccggctccac cggatctcgc gagggcagag gaagtcttct aacatgcggt 6361 gacgtggagg agaatcccgg ccctatgacc gagtacaagc ccacggtgcg cctcgccacc 6421 cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc 6481 acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc 6541 ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg 6601 gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc 6661 ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc 6721 ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc 6781 gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag 6841 cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag 6901 cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc 6961 atgacccgca agcccggtgc ctgaaatcaa cctctggatt acaaaatttg tgaaagattg 7021 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct 7081 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg 7141 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact 7201 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc 7261 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc 7321 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaA 7381 TCATCGTCCT TTCCTtggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc 7441 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg 7501 gctctgcggc ctcttccgcg tctTcgcctt cgccctcaga cgagtcggat ctccctttgg 7561 Gccgcctccc cgcctggtac ctttaagacc aatgacttac aaggcagctg tagatcttag 7621 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaaGataaga 7681 tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc 7741 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt 7801 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc 7861 agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt atttataact 7921 tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta 7981 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag 8041 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc taTCCCGCCC 8101 CTAACTCCGC CCAtcccgcc cctaactccg cccagttccg cccattctcc gccccatggc 8161 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag 8221 aagtagtgag gaggcttttt tggaggccta gacttttgca gagaccaaat tcgtaatcat 8281 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc 8341 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc 8401 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat 8461 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac 8521 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt 8581 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca 8641 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc 8701 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact 8761 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct 8821 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag 8881 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca 8941 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa 9001 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc 9061 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag 9121 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg 9181 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca 9241 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc 9301 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag 9361 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata 9421 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat 9481 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg 9541 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc 9601 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc 9661 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc 9721 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc 9781 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc 9841 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa 9901 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat 9961 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata 10021 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca 10081 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag 10141 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc 10201 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc 10261 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata 10321 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta 10381 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta 10441 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg 10501 tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt 10561 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg 10621 tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt 10681 gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg 10741 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct 10801 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg 10861 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.