pDEPTOR-Fluc-SV40-hRluc-Neo pl

pDEPTOR-Fluc-SV40-hRluc-Neo plasmid

PVT109407

pDEPTOR-Fluc-SV40-hRluc-Neo plasmid Information

Plasmid name: pDEPTOR(human)-Fluc-SV40-hRluc-Neo
Alias:
Gene lenght: 2002
Host: Mammalian cells
Use(s): Signal report,
Promoter detection
Fragment type: Promoter
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling: Fluc
Promoter: DEPTOR
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: cer region-F1
Reverse primer:
Copy number: High
Induction:

pDEPTOR-Fluc-SV40-hRluc-Neo plasmid Description

pDEPTOR-Fluc-SV40-hRluc-Neo is a plasmid suitable for promoter detection in mammalian cells.

pDEPTOR-Fluc-SV40-hRluc-Neo plasmid Sequence

1 CATGCAAGCT GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC 61 CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCGGCCGCT TCGAGCAGAC ATGATAAGAT 121 ACATTGATGA GTTTGGACAA ACCACAACTA GAATGCAGTG AAAAAAATGC TTTATTTGTG 181 AAATTTGTGA TGCTATTGCT TTATTTGTAA CCATTATAAG CTGCAATAAA CAAGTTAACA 241 ACAACAATTG CATTCATTTT ATGTTTCAGG TTCAGGGGGA GATGTGGGAG GTTTTTTTAA 301 GCAAGTAAAA CCTCTACAAA TGTGGTAAAA TCGAATTTTA ACAAAATATT AACGCTTACA 361 ATTTCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATACGCG 421 GATCTGCGCA GCACCATGGC CTGAAATAAC CTCTGAAAGA GGAACTTGGT TAGGTACCTT 481 CTGAGGCGGA AAGAACCAGC TGTGGAATGT GTGTCAGTTA GGGTGTGGAA AGTCCCCAGG 541 CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCAGGTGTGG 601 AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC 661 AACCATAGTC CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA ACTCCGCCCA GTTCCGCCCA 721 TTCTCCGCCC CATGGCTGAC TAATTTTTTT TATTTATGCA GAGGCCGAGG CCGCCTCGGC 781 CTCTGAGCTA TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA 841 GCTTGATTCT TCTGACACAA CAGTCTCGAA CCAAAGGCTG GAGCCACCAT GGCTTCCAAG 901 GTGTACGACC CCGAGCAACG CAAACGCATG ATCACTGGGC CTCAGTGGTG GGCTCGCTGC 961 AAGCAAATGA ACGTGCTGGA CTCCTTCATC AACTACTATG ATTCCGAGAA GCACGCCGAG 1021 AACGCCGTGA TTTTTCTGCA TGGTAACGCT GCCTCCAGCT ACCTGTGGAG GCACGTCGTG 1081 CCTCACATCG AGCCCGTGGC TAGATGCATC ATCCCTGATC TGATCGGAAT GGGTAAGTCC 1141 GGCAAGAGCG GGAATGGCTC ATATCGCCTC CTGGATCACT ACAAGTACCT CACCGCTTGG 1201 TTCGAGCTGC TGAACCTTCC AAAGAAAATC ATCTTTGTGG GCCACGACTG GGGGGCTTGT 1261 CTGGCCTTTC ACTACTCCTA CGAGCACCAA GACAAGATCA AGGCCATCGT CCATGCTGAG 1321 AGTGTCGTGG ACGTGATCGA GTCCTGGGAC GAGTGGCCTG ACATCGAGGA GGATATCGCC 1381 CTGATCAAGA GCGAAGAGGG CGAGAAAATG GTGCTTGAGA ATAACTTCTT CGTCGAGACC 1441 ATGCTCCCAA GCAAGATCAT GCGGAAACTG GAGCCTGAGG AGTTCGCTGC CTACCTGGAG 1501 CCATTCAAGG AGAAGGGCGA GGTTAGACGG CCTACCCTCT CCTGGCCTCG CGAGATCCCT 1561 CTCGTTAAGG GAGGCAAGCC CGACGTCGTC CAGATTGTCC GCAACTACAA CGCCTACCTT 1621 CGGGCCAGCG ACGATCTGCC TAAGATGTTC ATCGAGTCCG ACCCTGGGTT CTTTTCCAAC 1681 GCTATTGTCG AGGGAGCTAA GAAGTTCCCT AACACCGAGT TCGTGAAGGT GAAGGGCCTC 1741 CACTTCAGCC AGGAGGACGC TCCAGATGAA ATGGGTAAGT ACATCAAGAG CTTCGTGGAG 1801 CGCGTGCTGA AGAACGAGCA GACCGGTGGT GGGAGCGGAG GTGGCGGATC AGGTGGCGGA 1861 GGCTCCGGAG GGATTGAACA AGATGGATTG CACGCAGGTT CTCCGGCCGC TTGGGTGGAG 1921 AGGCTATTCG GCTATGACTG GGCACAACAG ACAATCGGCT GCTCTGATGC CGCCGTGTTC 1981 CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA CCGACCTGTC CGGTGCCCTG 2041 AATGAACTGC AGGACGAGGC AGCGCGGCTA TCGTGGCTGG CCACGACGGG CGTTCCTTGC 2101 GCAGCTGTGC TCGACGTTGT CACTGAAGCG GGAAGGGACT GGCTGCTATT GGGCGAAGTG 2161 CCGGGGCAGG ATCTCCTGTC ATCTCACCTT GCTCCTGCCG AGAAAGTATC CATCATGGCT 2221 GATGCAATGC GGCGGCTGCA TACGCTTGAT CCGGCTACCT GCCCATTCGA CCACCAAGCG 2281 AAACATCGCA TCGAGCGAGC ACGTACTCGG ATGGAAGCCG GTCTTGTCGA TCAGGATGAT 2341 CTGGACGAAG AGCATCAGGG GCTCGCGCCA GCCGAACTGT TCGCCAGGCT CAAGGCGCGC 2401 ATGCCCGACG GCGAGGATCT CGTCGTGACC CATGGCGATG CCTGCTTGCC GAATATCATG 2461 GTGGAAAATG GCCGCTTTTC TGGATTCATC GACTGTGGCC GGCTGGGTGT GGCGGACCGC 2521 TATCAGGACA TAGCGTTGGC TACCCGTGAT ATTGCTGAAG AGCTTGGCGG CGAATGGGCT 2581 GACCGCTTCC TCGTGCTTTA CGGTATCGCC GCTCCCGATT CGCAGCGCAT CGCCTTCTAT 2641 CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA CTCTGGGGTT CGAAATGACC GACCAAGCGA 2701 CGCCCAACCT GCCATCACGA TGGCCGCAAT AAAATATCTT TATTTTCATT ACATCTGTGT 2761 GTTGGTTTTT TGTGTGAATC GATAGCGATA ATCTTTGCGC TTGCGTTTTC CCTTGTCCAG 2821 ATAGCCCAGT AGCTGACATT CATCCGGGGT CAGCACCGTT TCTGCGGACT GGCTTTCTAC 2881 GTAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT 2941 TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA 3001 ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT 3061 ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA 3121 GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC 3181 AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTC 3241 AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTATTGACG CCGGGCAAGA GCAACTCGGT 3301 CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT 3361 CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC 3421 ACTGCGGCCA ACTTACTTCT GACAACTATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG 3481 CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC 3541 ATACCAAACG ACGAGCGTGA CACCACGATG CCTGTAGCAA TGGCAACAAC GTTGCGCAAA 3601 CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG 3661 GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT 3721 GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT 3781 GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA 3841 CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ATTCGAAATG 3901 ACCGACCAAG CGACGCCCAA CCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA 3961 CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA 4021 ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC 4081 ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG 4141 CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT 4201 ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT 4261 ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC 4321 AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG 4381 ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG 4441 GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGG ACAGTATTTG 4501 GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG 4561 GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA 4621 GAAAAAAAGG ATTTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA 4681 ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA 4741 TCCTTTTATA GTCCGGAAAT ACAGGAACGC ACGCTGGATG GCCCTTCGCT GGGATGGTGA 4801 AACCATGAAA AATGGCAGCT TCAGTGGATT AAGTGGGGGT AATGTGGCCT GTACCCTCTG 4861 GTTGCATAGG TATTCATACG GTTAAAATTT ATCAGGCGCG ATTGCGGCAG TTTTTCGGGT 4921 GGTTTGTTGC CATTTTTACC TGTCTGCTGC CGTGATCGCG CTGAACGCGT TTTAGCGGTG 4981 CGTACAATTA AGGGATTATG GTAAATCCAC TTACTGTCTG CCCTCGTAGC CATCGAGATA 5041 AACCGCAGTA CTCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTc gagctcaagc 5101 ttcgaattcG TTTTTTTTTT GTAGAGACAG GGTCTTGCTA TGTTGCCCAG GCTGGCATTG 5161 AACTCTTGGC CTCAAGCAGT CCTCCCACCC TAACTTCCCA AAGAACTGGG ATTACATTAT 5221 TTCATTTAAG CCACGCAACA TCCCTATGAA GTAGAAATTA TTATTCCTGT TTTATCGATA 5281 GGAAAGGGAG GCCAAGAGAA ATAACTAGCA TAAGGTTGCA AAATTAGCAA ACGACAGAAT 5341 TGAGATAGAA ATTGCATGTT TGACTGAGAA GCCCGTGCAA ACTCTTGATA TTCTATTTAG 5401 TAGTTATAAG ATTAAAACAT GAGCAACAGA CACCTTTTAA TCTCATTAAC TCTGGGACTT 5461 CTGTTGAATG ATTTATGTAT TTTATGCAGA ATAAGGCTCT TAGAATCACA GTCACCTAGA 5521 ACTATGTCTG CCTTGGTCTA CATGTCTATT GTGGCCTATT TAAAAAATTA AGAAACTTAA 5581 ATAATTTCTT TTGGCAAAAG AGTCACCTAA CATCTGTCTC ATGCAGATTC AGAGCTGGTC 5641 TGCTCCCGGA TGTTCAAGGG AGTTGGGCAA CTATTCTCTT TCTGGAGTGG GGCTGAGTAT 5701 GGCCAGGGTT TAAGGGCTAC CTAACTTTCT AGGCAGATTA CTTCATCTTT CTATACTTAA 5761 GCTTTCTCAT TTGTAAGTTG AAAATAATAC CAAACTCCAG GGTTTTGTGA GGATAAAGTG 5821 TTTGGCACAA TGTAAAGCAC AGAATAAATG CTCAACACAC ATGAATACTG CCATAAACAT 5881 TACTTCGCCT TCCTGCAGAC CAACTCCGAC TCTGCATGAA ACAGAAAATC AGAACATTTA 5941 TTTGGTACTA ATCAAATTTC TTTTCATAAT CTTTTACGGA TAGACAATAC CATTGTATCC 6001 CCATTTTAAG AGGCAGTCTT TCAGTCTTTT CAAAGCATAA CTTTGAAGCC AGAATTGCCT 6061 CGGTTAGATT TCTTCTTATT ATTATTATGA TGATTTGAGA CGGAGTCTCG CTCTTGTTGC 6121 CCAGGCTGGA ATGCAATGGC ACTAACTCCG CTCACCCGAA CTCGGCTTAC AGCAACCTCC 6181 GCCTCCCGGG TTCGAGCGAT TCTCCTGCCT CAGCCTCCGA AGTAGCTAGG ATTACAGGCA 6241 TGCACCACCA CGCCCGGCTA ATTTTGTATT TTTAGTAGAG ACGGGGTTTC TTCATGTTGG 6301 TCAGGCTGGT CTGGAACTCC TGACCTCAGG TGATCTGCCC GCCTCGGCCT CCCAAAGTGC 6361 TGGGATTACA GGCATGAGCC ACGGTGCCCG GCTCTTCTTA TACTTATTAT TTGCTTGCCT 6421 TAGACAAGTA ACTTCACCTC TCCAAGCCTG CATTCTAATG CAAAACGAAC ACCAATACCT 6481 ATTTTATGAA TTATTGTAAT AATTAAACGA GGTAATACCA TACCTGAAGT ATCTTTAATG 6541 CAGCAGATGT CAAAAGTGGT AGCCGCAATT ATTTTTATTT TGTAAGGGAG GATTAAACAC 6601 ATTTAAATAG GACATCACAG GAGCGGAGCT GGATCCCAGG TCAGCCTGAT TCCTAGGCCG 6661 GCGTTCCTTA TTGATTACAA CATAAGGCAA ACAAGTCGTC TTTGGAGAGA GAGGTTGGGG 6721 AGAGAAGAAT GTTTAATTTC TCCATGCCCT TCAATGACAT GTATAACCTT CACTGTTGAC 6781 CATTTGGCCA GATTGTTGAA TTATGTTTTG CTCTCTGGCA ACTTGGGGTG TGGAACGTAC 6841 AATAAAAACG CCTTCACACT AATAACCTCC AGGCCTGTCC CCAGGATTCT ACAGCATGAG 6901 CTTTGTGGGT AAAGAGAGCT CACCAAATTC AGCCTCCAGC AGCACACACA GCTCAGTAGC 6961 AGCGCCCCGC CCAGATGTTT ATATTTTCCT CCATCGGCCC CGCCCCCCGA CCATGAGGGG 7021 CTCTAGCCAC GCCCCCAAGC TCAAGTTTCT GGGGCCGGAC TAGCCCAGCG CGTCTGCCTA 7081 CCCATAGGGA TTCCCTCTCC AGCCAATCCA GggatccCAC CATGGAAGAT GCCAAAAACA 7141 TTAAGAAGGG CCCAGCGCCA TTCTACCCAC TCGAAGACGG GACCGCCGGC GAGCAGCTGC 7201 ACAAAGCCAT GAAGCGCTAC GCCCTGGTGC CCGGCACCAT CGCCTTTACC GACGCACATA 7261 TCGAGGTGGA CATTACCTAC GCCGAGTACT TCGAGATGAG CGTTCGGCTG GCAGAAGCTA 7321 TGAAGCGCTA TGGGCTGAAT ACAAACCATC GGATCGTGGT GTGCAGCGAG AATAGCTTGC 7381 AGTTCTTCAT GCCCGTGTTG GGTGCCCTGT TCATCGGTGT GGCTGTGGCC CCAGCTAACG 7441 ACATCTACAA CGAGCGCGAG CTGCTGAACA GCATGGGCAT CAGCCAGCCC ACCGTCGTAT 7501 TCGTGAGCAA GAAAGGGCTG CAAAAGATCC TCAACGTGCA AAAGAAGCTA CCGATCATAC 7561 AAAAGATCAT CATCATGGAT AGCAAGACCG ACTACCAGGG CTTCCAAAGC ATGTACACCT 7621 TCGTGACTTC CCATTTGCCA CCCGGCTTCA ACGAGTACGA CTTCGTGCCC GAGAGCTTCG 7681 ACCGGGACAA AACCATCGCC CTGATCATGA ACAGTAGTGG CAGTACCGGA TTGCCCAAGG 7741 GCGTAGCCCT ACCGCACCGC ACCGCTTGTG TCCGATTCAG TCATGCCCGC GACCCCATCT 7801 TCGGCAACCA GATCATCCCC GACACCGCTA TCCTCAGCGT GGTGCCATTT CACCACGGCT 7861 TCGGCATGTT CACCACGCTG GGCTACTTGA TCTGCGGCTT TCGGGTCGTG CTCATGTACC 7921 GCTTCGAGGA GGAGCTATTC TTGCGCAGCT TGCAAGACTA TAAGATTCAA TCTGCCCTGC 7981 TGGTGCCCAC ACTATTTAGC TTCTTCGCTA AGAGCACTCT CATCGACAAG TACGACCTAA 8041 GCAACTTGCA CGAGATCGCC AGCGGCGGGG CGCCGCTCAG CAAGGAGGTA GGTGAGGCCG 8101 TGGCCAAACG CTTCCACCTA CCAGGCATCC GCCAGGGCTA CGGCCTGACA GAAACAACCA 8161 GCGCCATTCT GATCACCCCC GAAGGGGACG ACAAGCCTGG CGCAGTAGGC AAGGTGGTGC 8221 CCTTCTTCGA GGCTAAGGTG GTGGACTTGG ACACCGGTAA GACACTGGGT GTGAACCAGC 8281 GCGGCGAGCT GTGCGTCCGT GGCCCCATGA TCATGAGCGG CTACGTTAAC AACCCCGAGG 8341 CTACAAACGC TCTCATCGAC AAGGACGGCT GGCTGCACAG CGGCGACATC GCCTACTGGG 8401 ACGAGGACGA GCACTTCTTC ATCGTGGACC GGCTGAAGAG CCTGATCAAA TACAAGGGCT 8461 ACCAGGTAGC CCCAGCCGAA CTGGAGAGCA TCCTGCTGCA ACACCCCAAC ATCTTCGACG 8521 CCGGGGTCGC CGGCCTGCCC GACGACGATG CCGGCGAGCT GCCCGCCGCA GTCGTCGTGC 8581 TGGAACACGG TAAAACCATG ACCGAGAAGG AGATCGTGGA CTATGTGGCC AGCCAGGTTA 8641 CAACCGCCAA GAAGCTGCGC GGTGGTGTTG TGTTCGTGGA CGAGGTGCCT AAAGGACTGA 8701 CCGGCAAGTT GGACGCCCGC AAGATCCGCG AGATTCTCAT TAAGGCCAAG AAGGGCGGCA 8761 AGATCGCCGT GTAATTCTAG TTGTTTAAAC GAGCTCGCTA GCCTCGAGTC TAGAGTCGAC 8821 CTGCAGG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.