pLV3-CMV-BNIP3-HA-mCherry-Blas

pLV3-CMV-BNIP3-HA-mCherry-Blast plasmid

PVT109305

pLV3-CMV-BNIP3-HA-mCherry-Blast plasmid Information

Plasmid name: pLV3-CMV-BNIP3(human)-HA-mCherry-Blast
Alias: NM_004052.4;HABON; NIP3
Gene lenght: 582
Host: Mammalian cells,lentivirus
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Blast
Fluorescent labeling: Red
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-BNIP3-HA-mCherry-Blast plasmid Description

pLV3-CMV-BNIP3-HA-mCherry-Blast It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-BNIP3-HA-mCherry-Blast plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc gaattcgcca ccatgtcgca gaacggagcg cccgggatgc aggaggagag 2341 cctgcagggc tcctgggtag aactgcactt cagcaataat gggaacgggg gcagcgttcc 2401 agcctcggtt tctatttata atggagacat ggaaaaaata ctgctggacg cacagcatga 2461 gtctggacgg agtagctcca agagctctca ctgtgacagc ccacctcgct cgcagacacc 2521 acaagatacc aacagagctt ctgaaacaga tacccatagc attggagaga aaaacagctc 2581 acagtctgag gaagatgata ttgaaagaag gaaagaagtt gaaagcatct tgaagaaaaa 2641 ctcagattgg atatgggatt ggtcaagtcg gccggaaaat attcccccca aggagttcct 2701 ctttaaacac ccgaagcgca cggccaccct cagcatgagg aacacgagcg tcatgaagaa 2761 agggggcata ttctctgcag aatttctgaa agttttcctt ccatctctgc tgctctctca 2821 tttgctggcc atcggattgg ggatctatat tggaaggcgt ctgacaacct ccaccagcac 2881 ctttggatcc TACCCATACG ACGTACCAGA TTACGCTtga gcggccgcGa aggatctgcg 2941 atcgctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg 3001 ggggaggggt cggcaattga acgggtgcct agagaaggtg gcgcggggta aactgggaaa 3061 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg 3121 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca cagctgaagc 3181 ttcgaggggc tcgcatctct ccttcacgcg cccgccgccc tacctgaggc cgccatccac 3241 gccggttgag tcgcgttctg ccgcctcccg cctgtggtgc ctcctgaact gcgtccgccg 3301 tctaggtaag tttaaagctc aggtcgagac cgggcctttg tccggcgctc ccttggagcc 3361 tacctagact cagccggctc tccacgcttt gcctgaccct gcttgctcaa ctctacgtct 3421 ttgtttcgtt ttctgttctg cgccgttaca gatccaagct gtgaccggcg cctacgctag 3481 acgccaccat ggtgagcaag ggcgaggagg ataacatggc catcatcaag gagttcatgc 3541 gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg 3601 gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc 3661 ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc aaggcctacg 3721 tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt 3781 gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc 3841 tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg 3901 gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg atgtaccccg 3961 aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact 4021 acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct 4081 acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac 4141 agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaagggat 4201 ctcgcgaggg cagaggaagt cttctaacat gcggtgacgt ggaggagaat cccggccctA 4261 TGGCCAAGCC TTTGTCTCAA GAAGAATCCA CCCTCATTGA AAGAGCAACG GCTACAATCA 4321 ACAGCATCCC CATCTCTGAA GACTACAGCG TCGCCAGCGC AGCTCTCTCT AGCGACGGCC 4381 GCATCTTCAC TGGTGTCAAT GTATATCATT TTACTGGGGG ACCTTGTGCA GAACTCGTGG 4441 TGCTGGGCAC TGCTGCTGCT GCGGCAGCTG GCAACCTGAC TTGTATCGTC GCGATCGGAA 4501 ATGAGAACAG GGGCATCTTG AGCCCCTGCG GACGGTGCCG ACAGGTGCTT CTCGATCTGC 4561 ATCCTGGGAT CAAAGCCATA GTGAAGGACA GTGATGGACA GCCGACGGCA GTTGGGATTC 4621 GTGAATTGCT GCCCTCTGGT TATGTGTGGG AGGGCTAAGT CGACaatcaa cctctggatt 4681 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg 4741 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct 4801 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc 4861 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca 4921 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac 4981 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt 5041 ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct 5101 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc 5161 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tctccgcctt cgccctcaga 5221 cgagtcggat ctccctttgg ccgcctcccc gcctggtacc tttaagacca atgacttaca 5281 aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc 5341 actcccaacg aaGataagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga 5401 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct 5461 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat 5521 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta 5581 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta 5641 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat 5701 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct 5761 ggctctagct atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg 5821 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa 5881 gtagtgagga ggcttttttg gaggcctaga cttttgcaga gacggcccaa attcgtaatc 5941 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg 6001 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat 6061 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg 6121 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct 6181 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc 6241 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg 6301 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg 6361 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg 6421 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac 6481 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca 6541 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt 6601 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc 6661 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag 6721 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac 6781 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt 6841 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa 6901 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg 6961 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa 7021 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat 7081 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc 7141 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat 7201 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc 7261 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc 7321 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag 7381 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg 7441 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg 7501 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag 7561 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt 7621 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga 7681 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc 7741 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc 7801 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc 7861 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc 7921 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca 7981 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat 8041 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt 8101 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt 8161 tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac 8221 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc 8281 gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag 8341 agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag 8401 gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc 8461 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc 8521 agggttttcc cagtcacgac gttgtaaaac gacggccagt gccaagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.