pBI121-NLS-EGFP-NLS plasmid

pBI121-NLS-EGFP-NLS plasmid

PVT109081

pBI121-NLS-EGFP-NLS plasmid Information

Plasmid name: pBI121-NLS-EGFP-NLS
Alias:
Gene lenght:
Host: Plant
Use(s): Gene expression,subcellular localization
Fragment type: ORF
Species: Empty Vector
Prokaryotic resistance: Kan
Screening marker: Neo/G418
Fluorescent labeling: Green
Promoter: 35s
Replicon: oriV
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: EGFP-F3
Reverse primer:
Copy number: Low
Induction:

pBI121-NLS-EGFP-NLS plasmid Description

pBI121-NLS-EGFP-NLS is a plasmid suitable for protein expression in plants, subcellular localization.

pBI121-NLS-EGFP-NLS plasmid Sequence

1 tgagcgtcgc aaaggcgctc ggtcttgcct tgctcgtcgg tgatgtactt caccagctcc 61 gcgaagtcgc tcttcttgat ggagcgcatg gggacgtgct tggcaatcac gcgcaccccc 121 cggccgtttt agcggctaaa aaagtcatgg ctctgccctc gggcggacca cgcccatcat 181 gaccttgcca agctcgtcct gcttctcttc gatcttcgcc agcagggcga ggatcgtggc 241 atcaccgaac cgcgccgtgc gcgggtcgtc ggtgagccag agtttcagca ggccgcccag 301 gcggcccagg tcgccattga tgcgggccag ctcgcggacg tgctcatagt ccacgacgcc 361 cgtgattttg tagccctggc cgacggccag caggtaggcc gacaggctca tgccggccgc 421 cgccgccttt tcctcaatcg ctcttcgttc gtctggaagg cagtacacct tgataggtgg 481 gctgcccttc ctggttggct tggtttcatc agccatccgc ttgccctcat ctgttacgcc 541 ggcggtagcc ggccagcctc gcagagcagg attcccgttg agcaccgcca ggtgcgaata 601 agggacagtg aagaaggaac acccgctcgc gggtgggcct acttcaccta tcctgcccgg 661 ctgacgccgt tggatacacc aaggaaagtc tacacgaacc ctttggcaaa atcctgtata 721 tcgtgcgaaa aaggatggat ataccgaaaa aatcgctata atgaccccga agcagggtta 781 tgcagcggaa aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg 841 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt 901 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag 961 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt 1021 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta 1081 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt 1141 cagtgagcga ggaagcggaa gagcgccaga aggccgccag agaggccgag cgcggccgtg 1201 aggcttggac gctagggcag ggcatgaaaa agcccgtagc gggctgctac gggcgtctga 1261 cgcggtggaa agggggaggg gatgttgtct acatggctct gctgtagtga gtgggttgcg 1321 ctccggcagc ggtcctgatc aatcgtcacc ctttctcggt ccttcaacgt tcctgacaac 1381 gagcctcctt ttcgccaatc catcgacaat caccgcgagt ccctgctcga acgctgcgtc 1441 cggaccggct tcgtcgaagg cgtctatcgc ggcccgcaac agcggcgaga gcggagcctg 1501 ttcaacggtg ccgccgcgct cgccggcatc gctgtcgccg gcctgctcct caagcacggc 1561 cccaacagtg aagtagctga ttgtcatcag cgcattgacg gcgtccccgg ccgaaaaacc 1621 cgcctcgcag aggaagcgaa gctgcgcgtc ggccgtttcc atctgcggtg cgcccggtcg 1681 cgtgccggca tggatgcgcg cgccatcgcg gtaggcgagc agcgcctgcc tgaagctgcg 1741 ggcattcccg atcagaaatg agcgccagtc gtcgtcggct ctcggcaccg aatgcgtatg 1801 attctccgcc agcatggctt cggccagtgc gtcgagcagc gcccgcttgt tcctgaagtg 1861 ccagtaaagc gccggctgct gaacccccaa ccgttccgcc agtttgcgtg tcgtcagacc 1921 gtctacgccg acctcgttca acaggtccag ggcggcacgg atcactgtat tcggctgcaa 1981 ctttgtcatg cttgacactt tatcactgat aaacataata tgtccaccaa cttatcagtg 2041 ataaagaatc cgcgcgttca atcggaccag cggaggctgg tccggaggcc agacgtgaaa 2101 cccaacatac ccctgatcgt aattctgagc actgtcgcgc tcgacgctgt cggcatcggc 2161 ctgattatgc cggtgctgcc gggcctcctg cgcgatctgg ttcactcgaa cgacgtcacc 2221 gcccactatg gcattctgct ggcgctgtat gcgttggtgc aatttgcctg cgcacctgtg 2281 ctgggcgcgc tgtcggatcg tttcgggcgg cggccaatct tgctcgtctc gctggccggc 2341 gccagatctg gggaaccctg tggttggcat gcacatacaa atggacgaac ggataaacct 2401 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc 2461 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga 2521 tcatgagcgg agaattaagg gagtcacgtt atgacccccg ccgatgacgc gggacaagcc 2581 gttttacgtt tggaactgac agaaccgcaa cgttgaagga gccactcagc cgcgggtttc 2641 tggagtttaa tgagctaagc acatacgtca gaaaccatta ttgcgcgttc aaaagtcgcc 2701 taaggtcact atcagctagc aaatatttct tgtcaaaaat gctccactga cgttccataa 2761 attcccctcg gtatccaatt agagtctcat attcactctc aatccaaata atctgcaccg 2821 gatctggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt 2881 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg 2941 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg 3001 gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg 3061 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg 3121 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca 3181 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc 3241 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc 3301 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca 3361 aggcgcgcat gcccgacggc gatgatctcg tcgtgaccca tggcgatgcc tgcttgccga 3421 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg 3481 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg 3541 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg 3601 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga 3661 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag 3721 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct 3781 catgctggag ttcttcgccc acgggatctc tgcggaacag gcggtcgaag gtgccgatat 3841 cattacgaca gcaacggccg acaagcacaa cgccacgatc ctgagcgaca atatgatcgg 3901 gcccggcgtc cacatcaacg gcgtcggcgg cgactgccca ggcaagaccg agatgcaccg 3961 cgatatcttg ctgcgttcgg atattttcgt ggagttcccg ccacagaccc ggatgatccc 4021 cgatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg ccggtcttgc 4081 gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta acatgtaatg 4141 catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat acatttaata 4201 cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc 4261 tatgttacta gatcgggcct cctgtcaatg ctggcggcgg ctctggtggt ggttctggtg 4321 gcggctctga gggtggtggc tctgagggtg gcggttctga gggtggcggc tctgagggag 4381 gcggttccgg tggtggctct ggttccggtg attttgatta tgaaaagatg gcaaacgcta 4441 ataagggggc tatgaccgaa aatgccgatg aaaacgcgct acagtctgac gctaaaggca 4501 aacttgattc tgtcgctact gattacggtg ctgctatcga tggtttcatt ggtgacgttt 4561 ccggccttgc taatggtaat ggtgctactg gtgattttgc tggctctaat tcccaaatgg 4621 ctcaagtcgg tgacggtgat aattcacctt taatgaataa tttccgtcaa tatttacctt 4681 ccctccctca atcggttgaa tgtcgccctt ttgtctttgg cccaatacgc aaaccgcctc 4741 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag 4801 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt 4861 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca 4921 caggaaacag ctatgaccat gattacgcca agcttgcatg cctgcaggtc cccagattag 4981 ccttttcaat ttcagaaaga atgctaaccc acagatggtt agagaggctt acgcagcagg 5041 tctcatcaag acgatctacc cgagcaataa tctccaggaa atcaaatacc ttcccaagaa 5101 ggttaaagat gcagtcaaaa gattcaggac taactgcatc aagaacacag agaaagatat 5161 atttctcaag atcagaagta ctattccagt atggacgatt caaggcttgc ttcacaaacc 5221 aaggcaagta atagagattg gagtctctaa aaaggtagtt cccactgaat caaaggccat 5281 ggagtcaaag attcaaatag aggacctaac agaactcgcc gtaaagactg gcgaacagtt 5341 catacagagt ctcttacgac tcaatgacaa gaagaaaatc ttcgtcaaca tggtggagca 5401 cgacacactt gtctactcca aaaatatcaa agatacagtc tcagaagacc aaagggcaat 5461 tgagactttt caacaaaggg taatatccgg aaacctcctc ggattccatt gcccagctat 5521 ctgtcacttt attgtgaaga tagtggaaaa ggaaggtggc tcctacaaat gccatcattg 5581 cgataaagga aaggccatcg ttgaagatgc ctctgccgac agtggtccca aagatggacc 5641 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt 5701 ggattgatgt gatatctcca atgacgtaag ggatgacgca caatcccact atccttcgca 5761 agacccttcc tctatataag gaagttcatt tcatttggag aggacctcga cctcaacaca 5821 acatatacaa aacaaacgaa tctcaagcaa tcaagcattc tacttctatt gcagcaattt 5881 aaatcatttc ttttaaagca aaagcaattt tctgaaaatt ttcaccattt acgaacgata 5941 ctcgagGCCA CCatggcgag ttcggaggtt tcaatgaaag gtaatcgtgg aggagataac 6001 ttctcctcct ctggttttag tgaccctaag gagactagaa atgtctccgt cgccggcgag 6061 gggcaaaaaa gtaattctac ccgatccgct gcagtcgacg tactagtgat ggtgagcaag 6121 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac 6181 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc 6241 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc 6301 ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc 6361 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac 6421 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc 6481 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac 6541 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg 6601 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag 6661 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc 6721 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc 6781 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaaggtacc cgggatcCAG 6841 CCTAAGAAGA AGAGAAAGGT TGGAGGAtaa ttgatcgata gagctcgaat ttccccgatc 6901 gTtcaaacat ttggcaataa agtttcttaa gattgaatcc tgttgccggT cttgcgatga 6961 ttatcatata atttctgttg aattacgtta aGcatgTaat aattaacatg taatgcatga 7021 cgttatttat gaGatgggtt tttatgatta Gagtcccgca attatacatt taatacgcga 7081 tagaaaacaa aatatagcgc gcaaactaGg ataaattatc gcgcgcggtg tcatctatgt 7141 tactagatcg ggaattcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc 7201 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa 7261 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcgc ccgctccttt 7321 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg 7381 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga 7441 tttgggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac 7501 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc 7561 tatctcgggc tattcttttg atttataagg gattttgccg atttcggaac caccatcaaa 7621 caggattttc gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc 7681 caggcggtga agggcaatca gctgttgccc gtctcactgg tgaaaagaaa aaccacccca 7741 gtacattaaa aacgtccgca atgtgttatt aagttgtcta agcgtcaatt tgtttacacc 7801 acaatatatc ctgccaccag ccagccaaca gctccccgac cggcagctcg gcacaaaatc 7861 accactcgat acaggcagcc catcagtccg ggacggcgtc agcgggagag ccgttgtaag 7921 gcggcagact ttgctcatgt taccgatgct attcggaaga acggcaacta agctgccggg 7981 tttgaaacac ggatgatctc gcggagggta gcatgttgat tgtaacgatg acagagcgtt 8041 gctgcctgtg atcaaatatc atctccctcg cagagatccg aattatcagc cttcttattc 8101 atttctcgct taaccgtgac aggctgtcga tcttgagaac tatgccgaca taataggaaa 8161 tcgctggata aagccgctga ggaagctgag tggcgctatt tctttagaag tgaacgttga 8221 cgatatcaac tcccctatcc attgctcacc gaatggtaca ggtcggggac ccgaagttcc 8281 gactgtcggc ctgatgcatc cccggctgat cgaccccaga tctggggctg agaaagccca 8341 gtaaggaaac aactgtaggt tcgagtcgcg agatcccccg gaaccaaagg aagtaggtta 8401 aacccgctcc gatcaggccg agccacgcca ggccgagaac attggttcct gtaggcatcg 8461 ggattggcgg atcaaacact aaagctactg gaacgagcag aagtcctccg gccgccagtt 8521 gccaggcggt aaaggtgagc agaggcacgg gaggttgcca cttgcgggtc agcacggttc 8581 cgaacgccat ggaaaccgcc cccgccaggc ccgctgcgac gccgacagga tctagcgctg 8641 cgtttggtgt caacaccaac agcgccacgc ccgcagttcc gcaaatagcc cccaggaccg 8701 ccatcaatcg tatcgggcta cctagcagag cggcagagat gaacacgacc atcagcggct 8761 gcacagcgcc taccgtcgcc gcgaccccgc ccggcaggcg gtagaccgaa ataaacaaca 8821 agctccagaa tagcgaaata ttaagtgcgc cgaggatgaa gatgcgcatc caccagattc 8881 ccgttggaat ctgtcggacg atcatcacga gcaataaacc cgccggcaac gcccgcagca 8941 gcataccggc gacccctcgg cctcgctgtt cgggctccac gaaaacgccg gacagatgcg 9001 ccttgtgagc gtccttgggg ccgtcctcct gtttgaagac cgacagccca atgatctcgc 9061 cgtcgatgta ggcgccgaat gccacggcat ctcgcaaccg ttcagcgaac gcctccatgg 9121 gctttttctc ctcgtgctcg taaacggacc cgaacatctc tggagctttc ttcagggccg 9181 acaatcggat ctcgcggaaa tcctgcacgt cggccgctcc aagccgtcga atctgagcct 9241 taatcacaat tgtcaatttt aatcctctgt ttatcggcag ttcgtagagc gcgccgtgcg 9301 tcccgagcga tactgagcga agcaagtgcg tcgagcagtg cccgcttgtt cctgaaatgc 9361 cagtaaagcg ctggctgctg aacccccagc cggaactgac cccacaaggc cctagcgttt 9421 gcaatgcacc aggtcatcat tgacccaggc gtgttccacc aggccgctgc ctcgcaactc 9481 ttcgcaggct tcgccgacct gctcgcgcca cttcttcacg cgggtggaat ccgatccgca 9541 catgaggcgg aaggtttcca gcttgagcgg gtacggctcc cggtgcgagc tgaaatagtc 9601 gaacatccgt cgggccgtcg gcgacagctt gcggtacttc tcccatatga atttcgtgta 9661 gtggtcgcca gcaaacagca cgacgatttc ctcgtcgatc aggacctggc aacgggacgt 9721 tttcttgcca cggtccagga cgcggaagcg gtgcagcagc gacaccgatt ccaggtgccc 9781 aacgcggtcg gacgtgaagc ccatcgccgt cgcctgtagg cgcgacaggc attcctcggc 9841 cttcgtgtaa taccggccat tgatcgacca gcccaggtcc tggcaaagct cgtagaacgt 9901 gaaggtgatc ggctcgccga taggggtgcg cttcgcgtac tccaacacct gctgccacac 9961 cagttcgtca tcgtcggccc gcagctcgac gccggtgtag gtgatcttca cgtccttgtt 10021 gacgtggaaa atgaccttgt tttgcagcgc ctcgcgcggg attttcttgt tgcgcgtggt 10081 gaacagggca gagcgggccg tgtcgtttgg catcgctcgc atcgtgtccg gccacggcgc 10141 aatatcgaac aaggaaagct gcatttcctt gatctgctgc ttcgtgtgtt tcagcaacgc 10201 ggcctgcttg gcctcgctga cctgttttgc caggtcctcg ccggcggttt ttcgcttctt 10261 ggtcgtcata gttcctcgcg tgtcgatggt catcgacttc gccaaacctg ccgcctcctg 10321 ttcgagacga cgcgaacgct ccacggcggc cgatggcgcg ggcagggcag ggggagccag 10381 ttgcacgctg tcgcgctcga tcttggccgt agcttgctgg accatcgagc cgacggactg 10441 gaaggtttcg cggggcgcac gcatgacggt gcggcttgcg atggtttcgg catcctcggc 10501 ggaaaacccc gcgtcgatca gttcttgcct gtatgccttc cggtcaaacg tccgattcat 10561 tcaccctcct tgcgggattg ccccgactca cgccggggca atgtgccctt attcctgatt 10621 tgacccgcct ggtgccttgg tgtccagata atccacctta tcggcaatga agtcggtccc 10681 gtagaccgtc tggccgtcct tctcgtactt ggtattccga atcttgccct gcacgaatac 10741 cagcgacccc ttgcccaaat acttgccgtg ggcctcggcc tgagagccaa aacacttgat 10801 gcggaagaag tcggtgcgct cctgcttgtc gccggcatcg ttgcgccaca tctaggtact 10861 aaaacaattc atccagtaaa atataatatt ttattttctc ccaatcaggc ttgatcccca 10921 gtaagtcaaa aaatagctcg acatactgtt cttccccgat atcctccctg atcgaccgga 10981 cgcagaaggc aatgtcatac cacttgtccg ccctgccgct tctcccaaga tcaataaagc 11041 cacttacttt gccatctttc acaaagatgt tgctgtctcc caggtcgccg tgggaaaaga 11101 caagttcctc ttcgggcttt tccgtcttta aaaaatcata cagctcgcgc ggatctttaa 11161 atggagtgtc ttcttcccag ttttcgcaat ccacatcggc cagatcgtta ttcagtaagt 11221 aatccaattc ggctaagcgg ctgtctaagc tattcgtata gggacaatcc gatatgtcga 11281 tggagtgaaa gagcctgatg cactccgcat acagctcgat aatcttttca gggctttgtt 11341 catcttcata ctcttccgag caaaggacgc catcggcctc actcatgagc agattgctcc 11401 agccatcatg ccgttcaaag tgcaggacct ttggaacagg cagctttcct tccagccata 11461 gcatcatgtc cttttcccgt tccacatcat aggtggtccc tttataccgg ctgtccgtca 11521 tttttaaata taggttttca ttttctccca ccagcttata taccttagca ggagacattc 11581 cttccgtatc ttttacgcag cggtattttt cgatcagttt tttcaattcc ggtgatattc 11641 tcattttagc catttattat ttccttcctc ttttctacag tatttaaaga taccccaaga 11701 agctaattat aacaagacga actccaattc actgttcctt gcattctaaa accttaaata 11761 ccagaaaaca gctttttcaa agttgttttc aaagttggcg tataacatag tatcgacgga 11821 gccgattttg aaaccacaat tatgggtgat gctgccaact tactgattta gtgtatgatg 11881 gtgtttttga ggtgctccag tggcttctgt gtctatcagc tgtccctcct gttcagctac 11941 tgacggggtg gtgcgtaacg gcaaaagcac cgccggacat cagcgctatc tctgctctca 12001 ctgccgtaaa acatggcaac tgcagttcac ttacaccgct tctcaacccg gtacgcacca 12061 gaaaatcatt gatatggcca tgaatggcgt tggatgccgg gcaacagccc gcattatggg 12121 cgttggcctc aacacgattt tacgtcactt aaaaaactca ggccgcagtc ggtaacctcg 12181 cgcatacagc cgggcagtga cgtcatcgtc tgcgcggaaa tggacgaaca gtggggctat 12241 gtcggggcta aatcgcgcca gcgctggctg ttttacgcgt atgacagtct ccggaagacg 12301 gttgttgcgc acgtattcgg tgaacgcact atggcgacgc tggggcgtct tatgagcctg 12361 ctgtcaccct ttgacgtggt gatatggatg acggatggct ggccgctgta tgaatcccgc 12421 ctgaagggaa agctgcacgt aatcagcaag cgatatacgc agcgaattga gcggcataac 12481 ctgaatctga ggcagcacct ggcacggctg ggacggaagt cgctgtcgtt ctcaaaatcg 12541 gtggagctgc atgacaaagt catcgggcat tatctgaaca taaaacacta tcaataagtt 12601 ggagtcatta cccaattatg atagaattta caagctataa ggttattgtc ctgggtttca 12661 agcattagtc catgcaagtt tttatgcttt gcccattcta tagatatatt gataagcgcg 12721 ctgcctatgc cttgccccct gaaatcctta catacggcga tatcttctat ataaaagata 12781 tattatctta tcagtattgt caatatattc aaggcaatct gcctcctcat cctcttcatc 12841 ctcttcgtct tggtagcttt ttaaatatgg cgcttcatag agtaattctg taaaggtcca 12901 attctcgttt tcatacctcg gtataatctt acctatcacc tcaaatggtt cgctgggttt 12961 atcgcacccc cgaacacgag cacggcaccc gcgaccacta tgccaagaat gcccaaggta 13021 aaaattgccg gccccgccat gaagtccgtg aatgccccga cggccgaagt gaagggcagg 13081 ccgccaccca ggccgccgcc ctcactgccc ggcacctggt cgctgaatgt cgatgccagc 13141 acctgcggca cgtcaatgct tccgggcgtc gcgctcgggc tgatcgccca tcccgttact 13201 gccccgatcc cggcaatggc aaggactgcc agcgctgcca tttttggggt gaggccgttc 13261 gcggccgagg ggcgcagccc ctggggggat gggaggcccg cgttagcggg ccgggagggt 13321 tcgagaaggg ggggcacccc ccttcggcgt gcgcggtcac gcgcacaggg cgcagccctg 13381 gttaaaaaca aggtttataa atattggttt aaaagcaggt taaaagacag gttagcggtg 13441 gccgaaaaac gggcggaaac ccttgcaaat gctggatttt ctgcctgtgg acagcccctc 13501 aaatgtcaat aggtgcgccc ctcatctgtc agcactctgc ccctcaagtg tcaaggatcg 13561 cgcccctcat ctgtcagtag tcgcgcccct caagtgtcaa taccgcaggg cacttatccc 13621 caggcttgtc cacatcatct gtgggaaact cgcgtaaaat caggcgtttt cgccgatttg 13681 cgaggctggc cagctccacg tcgccggccg aaatcgagcc tgcccctcat ctgtcaacgc 13741 cgcgccgggt gagtcggccc ctcaagtgtc aacgtccgcc cctcatctgt cagtgagggc 13801 caagttttcc gcgaggtatc cacaacgccg gcggccgcgg tgtctcgcac acggcttcga 13861 cggcgtttct ggcgcgtttg cagggccata gacggccgcc agcccagcgg cgagggcaac 13921 cagcccgg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.