pCMV-Tfeb-EGFP-Neo plasmid

pCMV-Tfeb-EGFP-Neo plasmid

PVT104432

pCMV-Tfeb-EGFP-Neo plasmid Information

Plasmid name: pCMV-Tfeb(mouse)-EGFP-Neo
Alias: NM_011549.4;bHLHe35; Tcfeb
Gene lenght: 1245
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Kan
Screening marker: Neo/G418
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-Tfeb-EGFP-Neo plasmid Description

pCMV-Tfeb-EGFP-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-Tfeb-EGFP-Neo plasmid Sequence

1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 601 ccggactcag atctcgagGC CACCatggcg tcacgcatcg ggctgcgcat gcagctcatg 661 cgggagcagg cccagcagga ggagcagcga gagcgcatgc agcagcaggc tgtcatgcat 721 tatatgcaac agcagcagca gcagcagcag cagctgggtg ggccccccac cccagccatc 781 aacacccctg tccacttcca gtcgcccccg cctgtgcccg gggaggtgct gaaggtgcag 841 tcctacctgg agaaccccac ctcctaccac ctgcaacagt cccagcatca gaaggttcgg 901 gagtatctgt ctgagaccta tgggaacaag tttgctgccc acgtgagccc agcccaaggt 961 tccccgaagc ctgccccagc agcatcccca ggggtgcggg ctggacacgt actgtccacc 1021 tcggccggca acagtgctcc caacagtccc atggccatgc tacatatcag ctccaacccc 1081 gagaaagagt ttgatgatgt cattgacaac attatgcgcc tggacagcgt gctgggctac 1141 atcaaccctg agatgcagat gcctaacacg ctgcccctgt ctagcagcca cctgaacgtg 1201 tacagcggtg acccccaggt cacagcctcc atggtgggtg tcaccagcag ctcctgccct 1261 gccgacctga ctcagaagcg agagctaaca gatgctgaga gcagagccct ggccaaggag 1321 cggcagaaga aagacaatca caacctaatt gagagaagac gcaggttcaa catcaatgac 1381 cggatcaagg agctgggaat gctgatcccc aaggccaacg acctggacgt gcgctggaac 1441 aaaggcacca tcctcaaggc ctctgtggat tacatccgga ggatgcagaa ggacctgcag 1501 aagtcccggg agctggagaa ccactcccgg cgcctggaga tgactaacaa gcagctctgg 1561 ctccgcatcc aggagctgga gatgcaggca cgcgtgcacg gcctccccac cacctcgccg 1621 tcgggtgtga atatggccga gctggcccag caggtggtga agcaagagtt gcccagtgag 1681 gatggcccag gggaggcgct gatgctgggg cctgaggtcc ctgagcctga gcaaatgccg 1741 gctcttcctc cccaggctcc gctgccctcg gccgcccagc cacagtctcc gttccatcac 1801 ctggacttca gccatggcct gagctttggg ggtgggggcg acgaggggcc cacaggttac 1861 cccgataccc tggggacaga gcacggctcc ccattcccca acctgtccaa gaaggatctg 1921 gacttaatgc tcctagatga ctccctgctc cccctggcct ctgaccccct cttttctacc 1981 atgtctcctg aggcctccaa ggccagcagc cgccggagca gcttcagcat ggaggagggt 2041 gatgttctga ccggtGGCGG TGGAGGAAGC ggcggaggtg gatctgatcc accggtcgcc 2101 accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg 2161 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc 2221 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc 2281 accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg 2341 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc 2401 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc 2461 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg 2521 cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga caagcagaag 2581 aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc 2641 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac 2701 cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg 2761 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag 2821 taaagcggcc gcgactctag atcataatca gccataccac atttgtagag gttttacttg 2881 ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg 2941 ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt 3001 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg 3061 tatcttaagg cgtaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt 3121 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa 3181 gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag 3241 aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt 3301 gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac 3361 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag 3421 gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg 3481 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc aggtggcact 3541 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg 3601 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt 3661 cctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag 3721 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg 3781 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag 3841 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc 3901 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg 3961 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaag 4021 atcgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca 4081 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc 4141 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc 4201 aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg 4261 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg 4321 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct 4381 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct 4441 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa 4501 gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa 4561 ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt gacccatggc 4621 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt 4681 ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct 4741 gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc 4801 gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg 4861 ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg 4921 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc 4981 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc tagggggagg ctaactgaaa 5041 cacggaagga gacaataccg gaaggaaccc gcgctatgac ggcaataaaa agacagaata 5101 aaacgcacgg tgttgggtcg tttgttcata aacgcggggt tcggtcccag ggctggcact 5161 ctgtcgatac cccaccgaga ccccattggg gccaatacgc ccgcgtttct tccttttccc 5221 caccccaccc cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag 5281 gccctgccat agcctcaggt tactcatata tactttagat tgatttaaaa cttcattttt 5341 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac 5401 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag 5461 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg 5521 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca 5581 gagcgcagat accaaatact gtTcttctag tgtagccgta gttaggccac cacttcaaga 5641 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca 5701 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc 5761 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca 5821 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa 5881 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc 5941 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc 6001 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg 6061 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat 6121 cccctgattc tgtggataac cgtattaccg ccatgcat

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.