pCMV-Ddx10-3×FLAG-Neo plasmid

pCMV-Ddx10-3×FLAG-Neo plasmid

PVT108851

pCMV-Ddx10-3×FLAG-Neo plasmid Information

Plasmid name: pCMV-Ddx10(mouse)-3×FLAG-Neo
Alias: NM_029936.2;4632415A01Rik
Gene lenght: 2625
Host: Mammalian cells
Use(s): Gene expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-Ddx10-3×FLAG-Neo plasmid Description

pCMV-Ddx10-3×FLAG-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-Ddx10-3×FLAG-Neo plasmid Sequence

1 GACGGATCGG GAGATCTCCC GATCCCCTAT GGTGCACTCT CAGTACAATC TGCTCTGATG 61 CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG 121 CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC 181 TTAGGGTTAG GCGTTTTGCG CTGCTTCGCG ATGTACGGGC CAGATATACG CGTTGACATT 241 GATTATTGAC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA 301 TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC 361 CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC 421 ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT 481 ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT 541 ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA 601 TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG 661 ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC 721 AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG 781 GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCT CTGGCTAACT AGAGAACCCA 841 CTGCTTACTG GCTTATCGAA ATTAATACGA CTCACTATAG GGAGACCCAA GCTGGCTAGC 901 GTTTAAACTT AAGCTTGGTA CCGAGCTCGG ATCCACTAGT CCAGTGTGGT GGAATTCgcc 961 accatgggca aaacggttgc ttctctgggt cagggaaccc gacccgaccc ggtgcggagc 1021 tttaatcgct ggaagaaaaa acacagccac aggcagcacc aaaagaagga gcggaggaag 1081 cagctaaaga agccggaatg gcaggtcgag cgcgagggga tcagccgcct catgcaaaac 1141 tacgagaaga taaatgtaaa tgaaattaca agattttcag atttcccttt gtccaaaaaa 1201 acattgaaag gtttgcaaga agcacagtac cgcttggtaa cagagataca gaagcagacc 1261 atagggttgg ctttgcaagg taaagatgtg cttggtgctg ccaaaactgg atctggcaag 1321 actttggctt ttcttgttcc ggtgttggaa gccttatacc gcctgcagtg gacttcaaca 1381 gatggcctgg gagttctgat aatatcacct actcgagaac tggcctatca gacatttgag 1441 gttctacgca aagtagggaa gaatcatgat ttttcagctg gtctcatcat tggtgggaag 1501 gatctgaaac atgaagctga gaggatcaac aacataaaca tactggtttg cacaccaggt 1561 cgacttcttc aacacatgga tgaaacaata tgttttcatg ccaccaatct tcagatgtta 1621 gttcttgatg aggcagatag aatcttggat atgggctttg ctgataccat gaatgctatt 1681 attgaaaatc ttcccaagaa acgccagact ttactgttct cagccacaca gaccaaatct 1741 gtaaaggacc ttgcgcgctt gagtttgaaa gaccctgagt atgtctgggt tcatgaaaaa 1801 gcaaaataca gcacccctgc tacgttggaa cagaactata taatttgtga gctgcaccaa 1861 aaaataagtg tgctgttttc ctttttgaga agccacctaa agaagaagag tattgtgttt 1921 ttctccagtt gcaaagaggt tcagtatctg tatagggtgt tctgcaggct gcgcccaggg 1981 atctcgatcc ttgctctcca tggccggcag cagcagatga gaagaatgga ggtctacaat 2041 gaatttgtcc gaaagagggc cgcagtactt tttgccactg atattgcagc taggggactg 2101 gattttccag ctgtgaattg ggtccttcag tttgattgtc cagaggatgc caacacgtat 2161 attcacagag ctggtagaac tgccaggtac aaagaagatg gagaagcgtt attaatttta 2221 ctaccttcag aagagcaagg aatggtgcag cagcttctcc agaagaaagt acctgtgaag 2281 gagatcaaaa tcaatccaga aaaacttatt gatgttcaga aaaaattaga atcattttta 2341 gctcaagatc aagatttaaa agaaagagct caaaggtgtt ttgtctccta tatacgctca 2401 gtatatctta tgaaggataa agaagtattt aatgtgagca agttacctat tactgaatat 2461 gcactgtctc ttggtcttgc tgtggcaccg cggataagat ttcttcagaa actggagaag 2521 cagcccagca cagaattggt aaagaatcca gtcacagaag cagttccgcc cagggccccc 2581 tccctcccca acgatgaagc agacgaatct ccagcttatg tcagtgagaa gatgtccgtc 2641 cttcacaaaa gtggggaaag actggaagag acagaacaca gactggctag tggtgacggt 2701 gacgaagaac aggatgaaga gacggaagac gaagagacgg aagatcatct gggaaaagca 2761 agagagcctc acactgaatc tgttgtcagt attgaggagg cacagaaggt caaggaagtt 2821 tctgtgcagt tcttaaacag agatgatgat gatgaagatg gacctgatgc tgacttcctc 2881 acagtgaagc ggcgggatgt gtttgggttg gatcttaaag agaatgaagc actgtcgaaa 2941 aaagaacctt ctaaatccag tgtcaagaaa aaattaacca aagttgcaga agcaaaaaaa 3001 gtaatgaaaa gaagttttaa agtgaacaag aaaataacat ttactgatga aggggagttg 3061 gttcagcagt ggccacagat tcagaaatgt gctataaaag atgtggagga agaagatgac 3121 actggtggta tcaacctaga caaagcaaag gaaaggcttc aggaagagga caaatttgac 3181 aaagaagaat acaggaagaa aattaaggca aagcaccggg aaagacggct aaaagaaaga 3241 gaggccagaa gagaagccaa caaaagacaa gcaaaggcca gagatgaaga ggaagccttt 3301 ctggactgga gtgatgaaga tgatggtggg tttgatccaa gtacacttcc agatcctgac 3361 aagcacagaa gttctgaaga gtcggagagt gaagatacaa accataagat gagtgacacc 3421 aagaagaagc aggaaacaag gaagagaaac aacactgaag acgatgatgt aagaccaaga 3481 agtcgtcatg gaaagaaggc caagtgggaa acggtagaac ctttggatac aggcctgtct 3541 ttagcagagg atgaagagct ggtgttacat ctcctcaaaa gtcaaaacCT CGAGGACTAC 3601 AAAGACCATG ACGGTGATTA TAAAGATCAT GACATCGACT ACAAGGATGA CGATGACAAG 3661 TAGTGAGGGC CCGTTTAAAC CCGCTGATCA GCCTCGACTG TGCCTTCTAG TTGCCAGCCA 3721 TCTGTTGTTT GCCCCTCCCC CGTGCCTTCC TTGACCCTGG AAGGTGCCAC TCCCACTGTC 3781 CTTTCCTAAT AAAATGAGGA AATTGCATCG CATTGTCTGA GTAGGTGTCA TTCTATTCTG 3841 GGGGGTGGGG TGGGGCAGGA CAGCAAGGGG GAGGATTGGG AAGACAATAG CAGGCATGCT 3901 GGGGATGCGG TGGGCTCTAT GGCTTCTGAG GCGGAAAGAA CCAGCTGGGG CTCTAGGGGG 3961 TATCCCCACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 4021 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 4081 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 4141 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 4201 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 4261 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 4321 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 4381 AAATTTAACG CGAATTAATT CTGTGGAATG TGTGTCAGTT AGGGTGTGGA AAGTCCCCAG 4441 GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCAGGTGTG 4501 GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG 4561 CAACCATAGT CCCGCCCCTA ACTCCGCCCA TCCCGCCCCT AACTCCGCCC AGTTCCGCCC 4621 ATTCTCCGCC CCATGGCTGA CTAATTTTTT TTATTTATGC AGAGGCCGAG GCCGCCTCTG 4681 CCTCTGAGCT ATTCCAGAAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA 4741 AGCTCCCGGG AGCTTGTATA TCCATTTTCG GATCTGATCA AGAGACAGGA TGAGGATCGT 4801 TTCGCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC 4861 TATTCGGCTA TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC 4921 TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG 4981 AACTGCAGGA CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG 5041 CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG 5101 GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG 5161 CAATGCGGCG GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC 5221 ATCGCATCGA GCGAGCACGT ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG 5281 ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGCGCATGC 5341 CCGACGGCGA GGATCTCGTC GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG 5401 AAAATGGCCG CTTTTCTGGA TTCATCGACT GTGGCCGGCT GGGTGTGGCG GACCGCTATC 5461 AGGACATAGC GTTGGCTACC CGTGATATTG CTGAAGAGCT TGGCGGCGAA TGGGCTGACC 5521 GCTTCCTCGT GCTTTACGGT ATCGCCGCTC CCGATTCGCA GCGCATCGCC TTCTATCGCC 5581 TTCTTGACGA GTTCTTCTGA GCGGGACTCT GGGGTTCGAA ATGACCGACC AAGCGACGCC 5641 CAACCTGCCA TCACGAGATT TCGATTCCAC CGCCGCCTTC TATGAAAGGT TGGGCTTCGG 5701 AATCGTTTTC CGGGACGCCG GCTGGATGAT CCTCCAGCGC GGGGATCTCA TGCTGGAGTT 5761 CTTCGCCCAC CCCAACTTGT TTATTGCAGC TTATAATGGT TACAAATAAA GCAATAGCAT 5821 CACAAATTTC ACAAATAAAG CATTTTTTTC ACTGCATTCT AGTTGTGGTT TGTCCAAACT 5881 CATCAATGTA TCTTATCATG TCTGTATACC GTCGACCTCT AGCTAGAGCT TGGCGTAATC 5941 ATGGTCATAG CTGTTTCCTG TGTGAAATTG TTATCCGCTC ACAATTCCAC ACAACATACG 6001 AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG TGCCTAATGA GTGAGCTAAC TCACATTAAT 6061 TGCGTTGCGC TCACTGCCCG CTTTCCAGTC GGGAAACCTG TCGTGCCAGC TGCATTAATG 6121 AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG CGCTCTTCCG CTTCCTCGCT 6181 CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 6241 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 6301 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 6361 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 6421 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 6481 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 6541 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 6601 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 6661 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 6721 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 6781 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 6841 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTTTTTTTG TTTGCAAGCA 6901 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 6961 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 7021 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 7081 TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT 7141 CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG 7201 GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG CGAGACCCAC GCTCACCGGC 7261 TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC 7321 AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC 7381 GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTACA GGCATCGTGG TGTCACGCTC 7441 GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC 7501 CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA 7561 GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT 7621 GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA 7681 GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAATA CGGGATAATA CCGCGCCACA 7741 TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG 7801 GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC 7861 AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC 7921 AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA 7981 TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA 8041 GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGTC

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.