pLV2-CMV-SDC4-EGFP-mCherry pla

pLV2-CMV-SDC4-EGFP-mCherry plasmid

PVT108787

pLV2-CMV-SDC4-EGFP-mCherry plasmid Information

Plasmid name: pLV2-CMV-SDC4(human)-EGFP-mCherry
Alias: NM_002999;SYND4
Gene lenght: 594
Host: Mammalian cells,lentivirus
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling: Red,Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV2-CMV-SDC4-EGFP-mCherry plasmid Description

pLV2-CMV-SDC4-EGFP-mCherry It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV2-CMV-SDC4-EGFP-mCherry plasmid Sequence

1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattcgc caccatggcc 2821 cccgcccgtc tgttcgcgct gctgctgttc ttcgtaggcg gagtcgccga gtcgatccga 2881 gagactgagg tcatcgaccc ccaggacctc ctagaaggcc gatacttctc cggagcccta 2941 ccagacgatg aggatgtagt ggggcccggg caggaatctg atgactttga gctgtctggc 3001 tctggagatc tggatgactt ggaagactcc atgatcggcc ctgaagttgt ccatcccttg 3061 gtgcctctag ataaccatat ccctgagagg gcagggtctg ggagccaagt ccccaccgaa 3121 cccaagaaac tagaggagaa tgaggttatc cccaagagaa tctcacccgt tgaagagagt 3181 gaggatgtgt ccaacaaggt gtcaatgtcc agcactgtgc agggcagcaa catctttgag 3241 agaacggagg tcctggcagc tctgattgtg ggtggcatcg tgggcatcct ctttgccgtc 3301 ttcctgatcc tactgctcat gtaccgtatg aagaagaagg atgaaggcag ctatgacctg 3361 ggcaagaaac ccatctacaa gaaagccccc accaatgagt tctacgcggg cggtggaggc 3421 agtggtggag gcggtagcat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc 3481 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc 3541 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg 3601 cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc 3661 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc 3721 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag 3781 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac 3841 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg 3901 gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac 3961 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg 4021 ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag 4081 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg 4141 gacgagctgt acaagtaagg atcccgcccc tctccctccc ccccccctaa cgttactggc 4201 cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg 4261 ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct 4321 aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca 4381 gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg 4441 aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct 4501 gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa 4561 tggctcacct caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt 4621 atgggatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa 4681 aacgtctagg ccccccgaac cacggggacg tggttttcct ttgaaaaaca cgatgataat 4741 atggtgagca agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag 4801 gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc 4861 cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc 4921 ttcgcctggg acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac 4981 cccgccgaca tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc 5041 gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac 5101 ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta 5161 atgcagaaga agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc 5221 gccctgaagg gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct 5281 gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc 5341 aacatcaagt tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa 5401 cgcgccgagg gccgccactc caccggcggc atggacgagc tgtacaagtg aacgcgtctg 5461 gaacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt 5521 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc 5581 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga 5641 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc 5701 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct 5761 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg 5821 gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct 5881 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc 5941 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg 6001 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggaat 6061 taattctgca gtcgagacct agaaaaacat ggagcaatca caagtagcaa tacagcagct 6121 accaatgctg attgtgcctg gctagaagca caagaggagg aggaggtggg ttttccagtc 6181 acacctcagg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt 6241 ttaaaagaaa agaggggact ggaagggcta attcactccc aacgaagaca agatatcctt 6301 gatctgtgga tctaccacac acaaggctac ttccctgatt agcagaacta cacaccaggg 6361 ccaggggtca gatatccact gacctttgga tggtgctaca agctagtacc agttgagcca 6421 gataaggtag aagaggccaa taaaggagag aacaccagct tgttacaccc tgtgagcctg 6481 catgggatgg atgacccgga gagagaagtg ttagagtgga ggtttgacag ccgcctagca 6541 tttcatcacg tggcccgaga gctgcatccg gagtacttca agaactgctg atatcgagct 6601 tgctacaagg gactttccgc tggggacttt ccagggaggc gtggcctggg cgggactggg 6661 gagtggcgag ccctcagatc ctgcatataa gcagctgctt tttgcctgta ctgggtctct 6721 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa 6781 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc 6841 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag 6901 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga 6961 gaggccttga cattgctagc gtttaccgtc gacctctagc tagagcttgg cgtaatcatg 7021 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc 7081 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc 7141 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat 7201 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac 7261 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt 7321 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca 7381 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc 7441 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact 7501 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct 7561 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag 7621 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca 7681 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa 7741 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc 7801 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag 7861 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg 7921 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca 7981 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc 8041 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag 8101 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata 8161 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat 8221 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg 8281 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc 8341 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc 8401 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc 8461 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc 8521 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc 8581 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa 8641 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat 8701 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata 8761 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca 8821 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag 8881 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc 8941 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc 9001 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata 9061 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta 9121 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcga 9181 cggatcggga gatcaacttg tttattgcag cttataatgg ttacaaataa agcaatagca 9241 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac 9301 tcatcaatgt atcttatcat gtctggatca actggataac tcaagctaac caaaatcatc 9361 ccaaacttcc caccccatac cctattacca ctgccaatta cctgtggttt catttactct 9421 aaacctgtga ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt taaatatgta 9481 ctacaaactt agtagt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.