pLV3-EF1a-SLC10A3-IRES-BSD pla

pLV3-EF1a-SLC10A3-IRES-BSD plasmid

PVT108632

pLV3-EF1a-SLC10A3-IRES-BSD plasmid Information

Plasmid name: pLV3-EF1a-SLC10A3(human)-IRES-BSD
Alias: NM_019848.5;DXS253E; P3
Gene lenght: 1434
Host: Mammalian cells,lentivirus
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Blast
Fluorescent labeling:
Promoter: EF1a
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: EF-1α-F1
Reverse primer:
Copy number: High
Induction:

pLV3-EF1a-SLC10A3-IRES-BSD plasmid Description

pLV3-EF1a-SLC10A3-IRES-BSD It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-EF1a-SLC10A3-IRES-BSD plasmid Sequence

1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg 3121 tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag 3181 actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag 3241 tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt 3301 caggtgtcgt gaaaactacc cctctagaGC CACCatggtg ttaatgcagg acaagggcag 3361 ctctcagcag tggcctggtc tggggggcga gggtggtggc acaggtccct taagcatgct 3421 cagagctgcc ctgctgctca tcagcctgcc atggggggcc caagggacag ccagcaccag 3481 cctcagcact gctgggggtc acaccgtgcc accgactggg ggccgctact tgagcattgg 3541 agatggctct gtgatggagt ttgagtttcc tgaggacagt gagggcatca tcgtgatctc 3601 cagccagtac ccaggccagg ccaacaggac ggcgcctggc cccatgctca gggtcacctc 3661 cctggacaca gaggtgctga ccatcaagaa cgtgagtgct ataacctggg gaggcggggg 3721 tggctttgtg gtgagcatcc actcaggcct ggctgggctg gccccactcc acatccagct 3781 cgtggacgcc catgaggccc cgcccacact gattgaggag cggagagact tctgcatcaa 3841 ggtctcacct gctgaagaca cgcctgccac cctcagcgcc gacctggccc acttctcgga 3901 aaacccaatc ctctacctgc tcctgcctct tatctttgtc aacaagtgtt cgtttgggtg 3961 caaagtggaa ctcgaggttc tgaaggggct catgcagagc ccccagccca tgctgctggg 4021 cctcctgggc cagtttctgg tcatgccctt gtacgctttc ctcatggcca aggtcttcat 4081 gctgcccaag gccctggctc tgggcctcat catcacctgc tcgtcgcctg gcggcggggg 4141 gagctacctc ttcagcctcc ttcttggagg ggacgtcacc ctggccatct ccatgacttt 4201 cctctctacg gtggctgcca ctggcttctt gcctctgtct tcggccatct acagccgcct 4261 gctcagcatc catgagacgc tccacgtgcc catctccaag atcctgggga ccctgctgtt 4321 cattgccatc cccatagccg tgggcgtgct gatcaagtcc aagctcccca agttctccca 4381 gctgctgctg caggtcgtca agcccttcag ctttgtgctc ctcctgggcg gcctcttcct 4441 ggcctatcgc atgggggtct tcatcctggc aggcatccgg ctacccatcg tactggtggg 4501 tatcacggtg cccctggttg gcctgttggt gggctactgc ctagccacgt gtctgaagct 4561 gccagtggcc cagcggcgga cggtcagcat tgaggtaggg gtgcagaaca gcctgctggc 4621 cttggccatg ctgcagctat ccctccgccg ccttcaagct gactatgcct cccaggcccc 4681 cttcattgtg gcgctgagcg gcacctccga gatgctggcc ttggtcattg gccacttcat 4741 ctacagcagc ctgttcccag ttccctgagg atccacgcgt ctccggccta gggataacag 4801 ggtaatccgc tagcccctct ccctcccccc cccctaacgt tactggccga agccgcttgg 4861 aataaggccg gtgtgcgttt gtctatatgt tattttccac catattgccg tcttttggca 4921 atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg ggtctttccc 4981 ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag 5041 cttcttgaag acaaacaacg tctgtagcga ccctttgcag gcagcggaac cccccacctg 5101 gcgacaggtg cctctgcggc caaaagccac gtgtataaga tacacctgca aaggcggcac 5161 aaccccagtg ccacgttgtg agttggatag ttgtggaaag agtcaaatgg ctctcctcaa 5221 gcgtattcaa caaggggctg aaggatgccc agaaggtacc ccattgtatg ggatctgatc 5281 tggggcctcg gtacacatgc tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc 5341 ccccgaacca cggggacgtg gttttccttt gaaaaacacg atgataatat ggccacacta 5401 gagatccacc ggtcgccacc atggccaagc ctttgtctca agaagaatcc accctcattg 5461 aaagagcaac ggctacaatc aacagcatcc ccatctctga agactacagc gtcgccagcg 5521 cagctctctc tagcgacggc cgcatcttca ctggtgtcaa tgtatatcat tttactgggg 5581 gaccttgtgc agaactcgtg gtgctgggca ctgctgctgc tgcggcagct ggcaacctga 5641 cttgtatcgt cgcgatcgga aatgagaaca ggggcatctt gagcccctgc ggacggtgcc 5701 gacaggtgct tctcgatctg catcctggga tcaaagccat agtgaaggac agtgatggac 5761 agccgacggc agttgggatt cgtgaattgc tgccctctgg ttatgtgtgg gagggctaag 5821 cggccgcgtc gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct 5881 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc 5941 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct 6001 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga 6061 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc 6121 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac 6181 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt 6241 tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt 6301 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc 6361 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc 6421 gcctggaatt cgagctcggt acctttaaga ccaatgactt acaaggcagc tgtagatctt 6481 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa 6541 gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc 6601 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa 6661 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag 6721 tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa 6781 cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt 6841 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct 6901 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc 6961 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg 7021 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc 7081 agaagtagtg aggaggcttt tttggaggcc tagggacgta cccaattcgc cctatagtga 7141 gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg 7201 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga 7261 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc 7321 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac 7381 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt 7441 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc 7501 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc 7561 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact 7621 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg 7681 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc 7741 gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc 7801 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa 7861 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc 7921 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa 7981 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa 8041 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg 8101 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa 8161 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc 8221 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc 8281 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta 8341 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag 8401 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca 8461 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata 8521 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc 8581 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca 8641 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca 8701 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg 8761 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa 8821 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt 8881 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat 8941 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg 9001 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga 9061 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac 9121 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt 9181 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag 9241 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc 9301 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag 9361 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca 9421 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt 9481 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc 9541 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc 9601 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc 9661 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa 9721 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac 9781 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc 9841 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa 9901 tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa 9961 agggaacaaa agctggagct gc

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.