pCMV-EGFP-IMPA1-Neo plasmid

pCMV-EGFP-IMPA1-Neo plasmid

PVT108504

pCMV-EGFP-IMPA1-Neo plasmid Information

Plasmid name: pCMV-EGFP-IMPA1(human)-Neo
Alias: NM 001144878.2;IMP; IMPA; MRT59
Gene lenght: 1011
Host: Mammalian cells
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Kan
Screening marker: Neo/G418
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: pEGFP-C-5'
Reverse primer:
Copy number: High
Induction:

pCMV-EGFP-IMPA1-Neo plasmid Description

pCMV-EGFP-IMPA1-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-EGFP-IMPA1-Neo plasmid Sequence

1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg 661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc 721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg 781 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc 841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag 901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag 961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac 1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac 1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc 1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg 1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc 1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag 1321 ctgtacaagt ccggactcag atctcgaggt ggaggcggat caggaggcgg tggatctatg 1381 gggcagcgcc caggtcctgt gctgcccgca gtcgcggtgc tgggacaggt ggcaaaaagg 1441 aaggtggcct ggctcctacg ctggaaggct gtgaccagga cagaaacagc aggaaacagt 1501 tcaggtgttt atggctttgg gaaaatgaaa atatttgtca aatattttca gaagatggct 1561 gatccttggc aggaatgcat ggattatgca gtaactctag caagacaagc tggagaggta 1621 gtttgtgaag ctataaaaaa tgaaatgaat gttatgctga aaagttctcc agttgatttg 1681 gtaactgcta cggaccaaaa agttgaaaaa atgcttatct cttccataaa ggaaaagtat 1741 ccatctcaca gtttcattgg tgaagaatct gtggcagctg gggaaaaaag tatcttaacc 1801 gacaacccca catggatcat tgaccctatt gatggaacaa ctaactttgt acatagattt 1861 ccttttgtag ctgtttcaat tggctttgct gtaaataaaa agatagaatt tggagttgtg 1921 tacagttgtg tggaaggcaa gatgtacact gccagaaaag gaaaaggtgc cttttgtaat 1981 ggtcaaaaac tacaagtttc acaacaagaa gatattacca aatctctctt ggtgactgag 2041 ttgggctctt ccagaacacc agagactgtg agaatggttc tttctaatat ggaaaagctt 2101 ttttgcattc ctgttcatgg gatccggagt gttggaacag cagctgttaa tatgtgcctt 2161 gtggcaactg gcggagcaga tgcatattat gaaatgggaa ttcactgctg ggatgttgca 2221 ggagctggca ttattgttac tgaagctggt ggcgtgctaa tggatgttac aggtggacca 2281 tttgatttga tgtcacgaag agtaattgct gcaaataata gaatattagc agaaaggata 2341 gctaaagaaa ttcaggttat acctttgcaa cgagacgacg aagattaaga attctgcagt 2401 cgacggtacc gcgggcccgg gatccaccgg atctagataa ctgatcataa tcagccatac 2461 cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa 2521 acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa 2581 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg 2641 tggtttgtcc aaactcatca atgtatctta acgcgtaaat tgtaagcgtt aatattttgt 2701 taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg 2761 gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt 2821 ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct 2881 atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt 2941 gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa 3001 agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc 3061 tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc 3121 tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat 3181 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc 3241 aataatattg aaaaaggaag agtcctgagg cggaaagaac cagctgtgga atgtgtgtca 3301 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct 3361 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca 3421 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc 3481 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta 3541 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt 3601 tggaggccta ggcttttgca aagatcgatc aagagacagg atgaggatcg tttcgcatga 3661 ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct 3721 atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc 3781 aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcaag 3841 acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg 3901 acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc 3961 tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc 4021 ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg 4081 agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc 4141 atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg 4201 aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc 4261 gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag 4321 cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg 4381 tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg 4441 agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc 4501 atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt 4561 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca 4621 ccctaggggg aggctaactg aaacacggaa ggagacaata ccggaaggaa cccgcgctat 4681 gacggcaata aaaagacaga ataaaacgca cggtgttggg tcgtttgttc ataaacgcgg 4741 ggttcggtcc cagggctggc actctgtcga taccccaccg agaccccatt ggggccaata 4801 cgcccgcgtt tcttcctttt ccccacccca ccccccaagt tcgggtgaag gcccagggct 4861 cgcagccaac gtcggggcgg caggccctgc catagcctca ggttactcat atatacttta 4921 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa 4981 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga 5041 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac 5101 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt 5161 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc 5221 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat 5281 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag 5341 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc 5401 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag 5461 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac 5521 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg 5581 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct 5641 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc 5701 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgccatgca 5761 t

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.