pLV-hU6-Negative-Control-sgRNA

pLV-hU6-Negative-Control-sgRNA-hUbC-dCas9-KRAB-T2a-Puro plasmid

PVT108112

pLV-hU6-Negative-Control-sgRNA-hUbC-dCas9-KRAB-T2a-Puro plasmid Information

Plasmid name: pLV-hU6-Negative-Control-sgRNA-hUbC-dCas9-KRAB-T2a-Puro
Alias: CACGTGTTTATGGGATGAAC
Gene lenght:
Host: Mammalian cells,lentivirus
Use(s): Gene knockout,negative control
Fragment type: CRISPR
Species: Empty Vector
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: U6
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: U6-F(Hairpin structure)
Reverse primer:
Copy number: High
Induction:

pLV-hU6-Negative-Control-sgRNA-hUbC-dCas9-KRAB-T2a-Puro plasmid Description

pLV-hU6-Negative-Control-sgRNA-hUbC-dCas9-KRAB-T2a-Puro This plasmid is suitable for control and gene knockout in mammalian cells and can also be used for lentivirus packaging.

pLV-hU6-Negative-Control-sgRNA-hUbC-dCas9-KRAB-T2a-Puro plasmid Sequence

1 CATGGTGGCT CTAGAGTCGA CCTGCAGCCC AAGCTTCGTC TAACAAAAAA GCCAAAAACG 61 GCCAGAATTT AGCGGACAAT TTACTAGTCT AACACTGAAA ATTACATATT GACCCAAATG 121 ATTACATTTC AAAAGGTGCC TAAAAAACTT CACAAAACAC ACTCGCCAAC CCCGAGCGCA 181 TAGTTCAAAA CCGGAGCTTC AGCTACTTAA GAAGATAGGT ACATAAAACC GACCAAAGAA 241 ACTGACGCCT CACTTATCCC TCCCCTCACC AGAGGTCCGG CGCCTGTCGA TTCAGGAGAG 301 CCTACCCTAG GCCCGAACCC TGCGTCCTGC GACGGAGAAA AGCCTACCGC ACACCTACCG 361 GCAGGTGGCC CCACCCTGCA TTATAAGCCA ACAGAACGGG TGACGTCACG ACACGACGAG 421 GGCGCGCGCT CCCAAAGGTA CGGGTGCACT GCCCAACGGC ACCGCCATAA CTGCCGCCCC 481 CGCAACAGAC GACAAACCGA GTTCTCCAGT CAGTGACAAA CTTCACGTCA GGGTCCCCAG 541 ATGGTGCCCC AGCCCATCTC ACCCGAATAA GAGCTTTCCC GCATTAGCGA AGGCCTCAAG 601 ACCTTGGGTT CTTGCCGCCC ACCATGCCCC CCACCTTGTT TCAACGACCT CACAGCCCGC 661 CTCACAAGCG TCTTCCATTC AAGACTCGGG AACAGCCGCC ATTTTGCTGC GCTCCCCCCA 721 ACCCCCAGTT CAGGGCAACC TTGCTCGCGG ACCCAGACTA CAGCCCTTGG CGGTCTCTCC 781 ACACGCTTCC GTCCCACCGA GCGGCCCGGC GGCCACGAAA GCCCCGGCCA GCCCAGCAGC 841 CCGCTACTCA CCAAGTGACG ATCACAGCGA TCCACAAACA AGAACCGCGA CCCAAATCCC 901 GGCTGCGACG GAACTAGCTG TGCCACACCC GGCGCGTCCT TATATAATCA TCGGCGTTCA 961 CCGCCCCACG GAGATCCCTC CGCAGAATCG CCGAGAAGGG ACTACTTTTC CTCGCCTGTT 1021 CCGCTCTCTG GAAAGAAAAC CAGTGCCCTA GAGTCACCCA AGTCCCGTCC TAAAATGTCC 1081 TTCTGCTGAT ACTGGGGTTC TAAGGCCGAG TCTTATGAGC AGCGGGCCGC TGTCCTGAGC 1141 GTCCGGGCGG AAGGATCAGG ACGCTCGCTG CGCCCTTCGT CTGACGTGGC AGCGCTCGCC 1201 GTGAGGAGGG GGGCGCCCGC GGGAGGCGCC AAAACCCGGC GCGGAGGCCA GATCTGGAGC 1261 CGACACGGGT TAATTAATTA AGAGGGCCTA TTTCCCATGA TTCCTTCATA TTTGCATATA 1321 CGATACAAGG CTGTTAGAGA GATAATTGGA ATTAATTTGA CTGTAAACAC AAAGATATTA 1381 GTACAAAATA CGTGACGTAG AAAGTAATAA TTTCTTGGGT AGTTTGCAGT TTTAAAATTA 1441 TGTTTTAAAA TGGACTATCA TATGCTTACC GTAACTTGAA AGTATTTCGA TTTCTTGGCT 1501 TTATATATCT TGTGGAAAGG ACGAAACACC GCACGTGTTT ATGGGATGAA CGTTTTAGAG 1561 CTAGAAATAG CAAGTTAAAA TAAGGCTAGT CCGTTATCAA CTTGAAAAAG TGGCACCGAG 1621 TCGGTGCTTT TTTTTTAATA ACTTCGTATA ATGTATGCTA TACGAAGTTA TTAATTAACC 1681 AAACTGGATC TCTGCTGTCC CTGTAATAAA CCCGAAAATT TTGAATTTTT GTAATTTGTT 1741 TTTGTAATTC TTTAGTTTGT ATGTCTGTTG CTATTATGTC TACTATTCTT TCCCCTGCAC 1801 TGTACCCCCC AATCCCCCCT TTTCTTTTAA AATTGTGGAT GAATACTGCC ATTTGTCTGC 1861 AGAATTGGCG CACGCAGTGC CGATCCGTTC ACTAATCGAA TGGATCTGTC TCTGTCTCTC 1921 TCTCCACCTT CTTCTTCTAT TCCTTCGGGC CTGTCGGGTC CCCTCGGGGT TGGGAGGTGG 1981 GTCTGAAACG ATAATGGTGA ATATCCCTGC CTAACTCTAT TCACTATAGA AAGTACAGCA 2041 AAAACTATTC TTAAACCTAC CAAGCCTCCT ACTATCATTA TGAATAATTT TATATACCAC 2101 AGCCAATTTG TTATGTTAAA CCAATTCCAC AAACTTGCCC ATTTATCTAA TTCCAATAAT 2161 TCTTGTTCAT TCTTTTCTTG CTGGTTTTGC GATTCTTCAA TTAAGGAGTG TATTAAGCTT 2221 GTGTAATTGT TAATTTCTCT GTCCCACTCC ATCCAGGTCG TGTGATTCCA AATCTGTTCC 2281 AGAGATTTAT TACTCCAACT AGCATTCCAA GGCACAGCAG TGGTGCAAAT GAGTTTTCCA 2341 GAGCAACCCC AAATCCCCAG GAGCTGTTGA TCCTTTAGGT ATCTTTCCAC AGCCAGGATT 2401 CTTGCCTGGA GCTGCTTGAT GCCCCAGACT GTGAGTTGCA ACAGATGCTG TTGCGCCTCA 2461 ATAGCCCTCA GCAAATTGTT CTGCTGCTGC ACTATACCAG ACAATAATTG TCTGGCCTGT 2521 ACCGTCAGCG TCATTGACGC TGCGCCCATA GTGCTTCCTG CTGCTCCCAA GAACCCAAGG 2581 AACAAAGCTC CTATTCCCAC TGCTCTTTTT TCTCTCTGCA CCACTCTTCT CTTTGCCTTG 2641 GTGGGTGCTA CTCCTAATGG TTCAATTTTT ACTACTTTAT ATTTATATAA TTCACTTCTC 2701 CAATTGTCCC TCATATCTCC TCCTCCAGGT CTGAAGATCA GCGGCCGCTT GCTGTGCGGT 2761 GGTCTTACTT TTGTTTTGCT CTTCCTCTAT CTTGTCTAAA GCTTCCTTGG TGTCTTTTAT 2821 CTCTATCCTT TGATGCACAC AATAGAGGGT TGCTACTGTA TTATATAATG ATCTAAGTTC 2881 TTCTGATCCT GTCTGAAGGG ATGGTTGTAG CTGTCCCAGT ATTTGTCTAC AGCCTTCTGA 2941 TGTTTCTAAC AGGCCAGGAT TAACTGCGAA TCGTTCTAGC TCCCTGCTTG CCCATACTAT 3001 ATGTTTTAAT TTATATTTTT TCTTTCCCCC TGGCCTTAAC CGAATTTTTT CCCATCGCGA 3061 TCTAATTCTC CCCCGCTTAA TACTGACGCT CTCGCACCCA TCTCTCTCCT TCTAGCCTCC 3121 GCTAGTCAAA ATTTTTGGCG TACTCACCAG TCGCCGCCCC TCGCCTCTTG CCGTGCGCGC 3181 TTCAGCAAGC CGAGTCCTGC GTCGAGAGAG CTCCTCTGGT TTCCCTTTCG CTTTCAAGTC 3241 CCTGTTCGGG CGCCACTGCT AGAGATTTTC CACACTGACT AAAAGGGTCT GAGGGATCTC 3301 TAGTTACCAG AGTCACACAA CAGACGGGCA CACACTACTT GAAGCACTCA AGGCAAGCTT 3361 TATTGAGGCT TAAGCAGTGG GTTCCCTAGT TAGCCAGAGA GCTCCCAGGC TCAGATCTGG 3421 TCTAACCAGA GAGACCCAGT ACAGGCAAAA CGCGCTGCTT ATATAGACCT CCCACCGTAC 3481 ACGCCTACCG CCCATTTGCG TCAATGGGGC GGAGTTGTTA CGACATTTTG GAAAGTCCCG 3541 TTGATTTTGG TGCCAAAACA AACTCCCATT GACGTCAATG GGGTGGAGAC TTGGAAATCC 3601 CCGTGAGTCA AACCGCTATC CACGCCCATT GATGTACTGC CAAAACCGCA TCACCATGGT 3661 AATAGCGATG ACTAATACGT AGATGTACTG CCAAGTAGGA AAGTCCCATA AGGTCATGTA 3721 CTGGGCATAA TGCCAGGCGG GCCATTTACC GTCATTGACG TCAATAGGGG GCGTACTTGG 3781 CATATGATAC ACTTGATGTA CTGCCAAGTG GGCAGTTTAC CGTAAATACT CCACCCATTG 3841 ACGTCAATGG AAAGTCCCTA TTGGCGTTAC TATGGGAACA TACGTCATTA TTGACGTCAA 3901 TGGGCGGGGG TCGTTGGGCG GTCAGCCAGG CGGGCCATTT ACCGTAAGTT ATGTAACGCG 3961 GAACTCCATA TATGGGCTAT GAACTAATGA CCCCGTAATT GATTACTATT AATAACTAGT 4021 CAATAATCAA TGTCAACGCG TATATCTGGC CCGTACATCG CGAAGCAGCG CAAAACGCCT 4081 AACCCTAAGC AGATTCTTCA TGCAATTGTC GGTCAAGCCT TGCCTTGTTG TAGCTTAAAT 4141 TTTGCTCGCG CACTACTCAG CGACCTCCAA CACACAAGCA GGGAGCAGAT ACTGGCTTAA 4201 CTATGCGGCA TCAGAGCAGA TTGTACTGAG AGTGCACCAT AGGGGATCGG GAGATCTCCC 4261 GATCCGTCGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA 4321 TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT 4381 CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC 4441 TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA 4501 GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT 4561 AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT 4621 CTGCTATGTG GCGCGGTATT ATCCCGTATT GACGCCGGGC AAGAGCAACT CGGTCGCCGC 4681 ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG 4741 GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG 4801 GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC 4861 ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA 4921 AACGACGAGC GTGACACCAC GATGCCTGTA GCAATGGCAA CAACGTTGCG CAAACTATTA 4981 ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT 5041 AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA 5101 TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG 5161 CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT 5221 AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT 5281 TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG 5341 AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA 5401 GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA 5461 ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA 5521 GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT 5581 GTTCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA 5641 TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT 5701 ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG 5761 GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG 5821 CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA 5881 AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT 5941 CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG 6001 TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC 6061 TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC 6121 CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC 6181 GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CCAATACGCA AACCGCCTCT CCCCGCGCGT 6241 TGGCCGATTC ATTAATGCAG CTGGCACGAC AGGTTTCCCG ACTGGAAAGC GGGCAGTGAG 6301 CGCAACGCAA TTAATGTGAG TTAGCTCACT CATTAGGCAC CCCAGGCTTT ACACTTTATG 6361 CTTCCGGCTC GTATGTTGTG TGGAATTGTG AGCGGATAAC AATTTCACAC AGGAAACAGC 6421 TATGACCATG ATTACGCCAA GCTCTAGCTA GAGGTCGACG GTATACAGAC ATGATAAGAT 6481 ACATTGATGA GTTTGGACAA ACCACAACTA GAATGCAGTG AAAAAAATGC TTTATTTGTG 6541 AAATTTGTGA TGCTATTGCT TTATTTGTAA CCATTATAAG CTGCAATAAA CAAGTTGGGG 6601 TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG 6661 AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTAGC 6721 ACGTGTCAGT CCTGCTCCTC GGCCACGAAG TGCACGCAGT TGCCGGCCGG GTCGCGCAGG 6781 GCGAACTCCC GCCCCCACGG CTGCTCGCCG ATCTCGGTCA TGGCCGGCCC GGAGGCGTCC 6841 CGGAAGTTCG TGGACACGAC CTCCGACCAC TCGGCGTACA GCTCGTCCAG GCCGCGCACC 6901 CACACCCAGG CCAGGGTGTT GTCCGGCACC ACCTGGTCCT GGACCGCGCT GATGAACAGG 6961 GTCACGTCGT CCCGGACCAC ACCGGCGAAG TCGTCCTCCA CGAAGTCCCG GGAGAACCCG 7021 AGCCGGTCGG TCCAGAACTC GACCGCTCCG GCGACGTCGC GCGCGGTGAG CACCGGAACG 7081 GCACTGGTCA ACTTGGCCAT GGTTTAGTTC CTCACCTTGT CGTATTATAC TATGCCGATA 7141 TACTATGCCG ATGATTAATT GTCAACACGT GCTGATCAGA TCCGAAAATG GATATACAAG 7201 CTCCCGGGAG CTTTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA 7261 ATAGCTCAGA GGCAGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT 7321 GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG 7381 GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC 7441 CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC 7501 CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGAATTAATT 7561 CGCGTTAAAT TTTTGTTAAA TCAGCTCATT TTTTAACCAA TAGGCCGAAA TCGGCAAAAT 7621 CCCTTATAAA TCAAAAGAAT AGACCGAGAT AGGGTTGAGT GTTGTTCCAG TTTGGAACAA 7681 GAGTCCACTA TTAAAGAACG TGGACTCCAA CGTCAAAGGG CGAAAAACCG TCTATCAGGG 7741 CGATGGCCCA CTACGTGAAC CATCACCCTA ATCAAGTTTT TTGGGGTCGA GGTGCCGTAA 7801 AGCACTAAAT CGGAACCCTA AAGGGAGCCC CCGATTTAGA GCTTGACGGG GAAAGCCGGC 7861 GAACGTGGCG AGAAAGGAAG GGAAGAAAGC GAAAGGAGCG GGCGCTAGGG CGCTGGCAAG 7921 TGTAGCGGTC ACGCTGCGCG TAACCACCAC ACCCGCCGCG CTTAATGCGC CGCTACAGGG 7981 CGCGTGGGGA TACCCCCTAG AGCCCCAGCT GGTTCTTTCC GCCTCAGAAG CCATAGAGCC 8041 CACCGCATCC CCAGCATGCC TGCTATTGTC TTCCCAATCC TCCCCCTTGC TGTCCTGCCC 8101 CACCCCACCC CCCAGAATAG AATGACACCT ACTCAGACAA TGCGATGCAA TTTCCTCATT 8161 TTATTAGGAA AGGACAGTGG GAGTGGCACC TTCCAGGGTC AAGGAAGGCA CGGGGGAGGG 8221 GCAAACAACA GATGGCTGGC AACTAGAAGG CACAGTCGAG GCTGATCAGC GGGTTTAAAC 8281 GGGCCCTGCT AGAGATTTTC CACACTGACT AAAAGGGTCT GAGGGATCTC TAGTTACCAG 8341 AGTCACACAA CAGACGGGCA CACACTACTT GAAGCACTCA AGGCAAGCTT TATTGAGGCT 8401 TAAGCAGTGG GTTCCCTAGT TAGCCAGAGA GCTCCCAGGC TCAGATCTGG TCTAACCAGA 8461 GAGACCCAGT ACAGTCCGGA TGCAGCTCTC GGGCCATGTG ATGAAATGCT AGGCGGCTGT 8521 CAAACCTCCA CTCTAATACT TCTCTCTCCG GGTCATCCAT CCCATGCAGG CTCACAGGGT 8581 GTAACAAGCG GGTGTTCTCT CCTTCATTGG CTTCTTCTAC CTTCTCTTGC TCAACTGGTA 8641 CTAGCTTGTA GCACCATCCA AAGGTCAGTG GATATCTGAT CCCTGGCCCT GGTGTGTAGT 8701 TCTGCCAATC AGGGAAGTAG CCTTGTGTGT GGTAGATCCA CAGATCAAGG ATATCTTGTC 8761 TTCGTTGGGA GTGAATTAGC CCTTCCAGTC CCCCCTTTTC TTTTAAAAAG TGGCTAAGAT 8821 CTACAGCTGC CTTGTAAGTC ATTGGTCTTA AAGGTACCTG AGGTGTGACT GGAAAACCCA 8881 CCTCCTCCTC CTCTTGTGCT TCTAGCCAGG CACAATCAGC ATTGGTAGCT GCTGTATTGC 8941 TACTTGTGAT TGCTCCATGT TTTTCTAGGT CTCGAGGTCG ACGGTATCGA TGCGGGGAGG 9001 CGGCCCAAAG GGAGATCCGA CTCGTCTGAG GGCGAAGGCG AAGACGCGGA AGAGGCCGCA 9061 GAGCCGGCAG CAGGCCGCGG GAAGGAAGGT CCGCTGGATT GAGGGCCGAA GGGACGTAGC 9121 AGAAGGACGT CCCGCGCAGA ATCCAGGTGG CAACACAGGC GAGCAGCCAA GGAAAGGACG 9181 ATGATTTCCC CGACAACACC ACGGAATTGT CAGTGCCCAA CAGCCGAGCC CCTGTCCAGC 9241 AGCGGGCAAG GCAGGCGGCG ATGAGTTCCG CCGTGGCAAT AGGGAGGGGG AAAGCGAAAG 9301 TCCCGGAAAG GAGCTGACAG GTGGTGGCAA TGCCCCAACC AGTGGGGGTT GCGTCAGCAA 9361 ACACAGTGCA CACCACGCCA CGTTGCCTGA CAACGGGCCA CAACTCCTCA TAAAGAGACA 9421 GCAACCAGGA TTTATACAAG GAGGAGAAAA TGAAAGCCAT ACGGGAAGCA ATAGCATGAT 9481 ACAAAGGCAT TAAAGCAGCG TATCCACATA GCGTAAAAGG AGCAACATAG TTAAGAATAC 9541 CAGTCAATCT TTCACAAATT TTGTAATCCA GAGGTTGATT ATCGATAAGC TTGATATCGA 9601 ATTCATAACT TCGTATAATG TATGCTATAC GAAGTTATCT ATTATCAACC GGTCGTGCTG 9661 CAGAAGCTAG TAACGGCCGC CAGTGTGCTG GTCAGGCACC GGGCTTGCGG GTCATGCACC 9721 AGGTGCGCGG TCCTTCGGGC ACCTCGACGT CGGCGGTGAC GGTGAAGCCG AGCCGCTCGT 9781 AGAAGGGGAG GTTGCGGGGC GCGGAGGTCT CCAGGAAGGC GGGCACCCCG GCGCGCTCGG 9841 CCGCCTCCAC TCCGGGGAGC ACGACGGCGC TGCCCAGACC CTTGCCCTGG TGGTCGGGCG 9901 ACACGCCGAC GGTGGCCAGG AACCACGCGG GCTCCTTGGG CCGGTGCGGC GCCAGGAGGC 9961 CTTCCATCTG TTGCTGCGCG GCCAGCCGGG AACCGCTCAA CTCGGCCATG CGCGGGCCGA 10021 TCTCGGCGAA CACCGCCCCC GCTTCGACGC TCTCCGGCGT GGTCCAGACC GCCACCGCGG 10081 CGCCGTCGTC CGCGACCCAC ACCTTGCCGA TGTCGAGCCC GACGCGCGTG AGGAAGAGTT 10141 CTTGCAGCTC GGTGACCCGC TCGATGTGGC GGTCCGGATC GACGGTGTGG CGCGTGGCGG 10201 GGTAGTCGGC GAACGCGGCG GCGAGGGTGC GTACGGCCCT GGGGACGTCG TCGCGGGTGG 10261 CGAGGCGCAC CGTGGGCTTG TACTCGGTCA TAGGGCCGGG ATTCTCCTCC ACGTCACCGC 10321 ATGTTAGAAG ACTTCCTCTG CCCTCGCTAG CAACTTTGCG TTTCTTTTTC GGAACTGATG 10381 ATTTGATTTC AAATGCAGTC TCTGAATCAG GATGGGTCTC TTGGTGAATT TCTCTCTCCA 10441 CCAGCCAGGG CTCTTCTCCC TTCTCCAACC GGAGGATCAC ATCTGGCTTA GTAAGCTGAT 10501 AACCCAAGGA AACCAGGTTC TTATAGTTCT CCAGCATCAC ATTTCTGTAC AGGATCTGCT 10561 GAGCAGTGTC CAGCAGCTTC CACTCCTCCC TGGTGAAGTC CACAAACACA TCCTTGAAGG 10621 TCACCAGTGT CCGGGACCAG GCAGTCAGTG ACTTAGCATC GCTAGCCACC TTCCTCTTCT 10681 TCTTGGGGTC AGCCCTGCTG TCTCCACCGA GCTGAGAGAG GTCGATTCTT GTTTCATAGA 10741 GCCCCGTAAT TGACTGATGA ATCAGTGTGG CGTCCAGGAC CTCCTTTGTA GAGGTGTACC 10801 GCTTTCTGTC TATGGTGGTG TCGAAGTACT TGAAGGCTGC AGGCGCGCCC AAGTTGGTCA 10861 GAGTAAACAA GTGGATAATG TTTTCTGCCT GCTCCCTGAT GGGCTTATCC CTGTGCTTAT 10921 TGTAAGCAGA AAGCACCTTA TCGAGGTTAG CGTCGGCGAG GATCACTCTT TTGGAGAATT 10981 CGCTTATTTG CTCGATGATC TCATCAAGGT AGTGTTTGTG TTGTTCCACG AACAGCTGCT 11041 TCTGCTCATT ATCTTCGGGA GACCCTTTGA GCTTTTCATA GTGGCTGGCC AGATACAAGA 11101 AATTAACGTA TTTAGAGGGC AGTGCCAGCT CGTTACCTTT CTGCAGCTCG CCCGCACTAG 11161 CGAGCATTCG TTTCCGGCCG TTTTCAAGCT CAAAGAGAGA GTACTTGGGA AGCTTAATGA 11221 TGAGGTCTTT TTTGACCTCT TTATATCCTT TCGCCTCGAG AAAGTCGATG GGGTTTTTTT 11281 CGAAGCTTGA TCGCTCCATG ATTGTGATGC CCAGCAGTTC CTTGACGCTT TTGAGTTTTT 11341 TAGACTTCCC TTTCTCCACT TTGGCCACAA CCAGTACACT GTAAGCGACT GTAGGAGAAT 11401 CGAATCCGCC GTATTTCTTG GGGTCCCAAT CTTTTTTGCG TGCGATCAGC TTGTCGCTGT 11461 TCCTTTTCGG GAGGATACTT TCCTTGGAGA AGCCTCCGGT CTGTACTTCG GTCTTTTTAA 11521 CGATGTTCAC CTGCGGCATG GACAGGACCT TCCGGACTGT CGCGAAATCC CTACCCTTGT 11581 CCCACACGAT TTCTCCTGTT TCTCCGTTTG TTTCGATAAG TGGTCGCTTC CGAATCTCTC 11641 CATTGGCCAG TGTAATCTCG GTCTTGAAAA AATTCATAAT ATTGCTGTAA AAGAAGTACT 11701 TAGCGGTGGC CTTGCCTATT TCCTGCTCAG ACTTTGCGAT CATTTTCCTA ACATCGTACA 11761 CTTTATAGTC TCCGTAAACA AATTCAGATT CAAGCTTGGG ATATTTTTTG ATAAGTGCAG 11821 TGCCTACCAC TGCATTCAGG TAGGCATCAT GCGCATGGTG GTAATTGTTG ATCTCTCTCA 11881 CCTTATAAAA CTGAAAGTCC TTTCTGAAAT CTGAGACCAG CTTAGACTTC AGAGTAATAA 11941 CTTTCACCTC TCGAATCAGT TTGTCATTTT CATCGTACTT GGTGTTCATG CGTGAATCGA 12001 GAATTTGGGC CACGTGCTTG GTGATCTGGC GTGTCTCAAC AAGCTGCCTT TTGATGAAGC 12061 CGGCTTTATC CAACTCAGAC AGGCCACCTC GTTCAGCCTT AGTCAGATTA TCGAACTTCC 12121 GTTGTGTGAT CAGTTTGGCG TTCAGCAGCT GCCGCCAATA ATTTTTCATT TTCTTGACAA 12181 CTTCTTCTGA GGGGACGTTA TCACTCTTCC CTCTATTTTT ATCGGATCTT GTCAACACTT 12241 TATTATCAAT AGAATCATCT TTGAGAAAAG ACTGGGGCAC GATGGCATCC ACGTCGTAGT 12301 CGGAGAGCCG ATTGATGTCC AGTTCCTGAT CCACGTACAT GTCCCTGCCG TTCTGCAGGT 12361 AGTACAGGTA GAGCTTCTCA TTCTGAAGCT GGGTGTTTTC AACTGGGTGT TCCTTAAGGA 12421 TTTGGGACCC CAGTTCTTTT ATACCCTCTT CAATCCTCTT CATCCTTTCC CTACTGTTCT 12481 TCTGTCCCTT CTGGGTAGTT TGGTTCTCTC GGGCCATCTC GATAACGATA TTCTCGGGCT 12541 TATGCCTTCC CATTACTTTG ACGAGTTCAT CCACGACCTT AACGGTCTGC AGTATTCCCT 12601 TTTTGATAGC TGGGCTACCT GCAAGATTAG CGATGTGCTC GTGAAGACTG TCCCCCTGGC 12661 CAGAAACTTG TGCTTTCTGG ATGTCCTCCT TAAAGGTGAG AGAGTCATCA TGGATCAACT 12721 GCATGAAGTT CCGGTTGGCA AATCCATCGG ACTTAAGAAA ATCCAGGATT GTCTTTCCAC 12781 TCTGCTTGTC TCGGATCCCA TTGATCAGTT TTCTTGACAG CCGCCCCCAT CCTGTATATC 12841 GGCGCCTCTT GAGCTGTTTC ATGACTTTGT CGTCGAAGAG ATGAGCGTAA GTTTTCAAGC 12901 GTTCTTCAAT CATCTCCCTA TCTTCAAACA ACGTAAGGGT GAGGACAATG TCCTCAAGAA 12961 TGTCCTCGTT CTCCTCATTG TCCAGGAAGT CCTTGTCTTT AATGATTTTC AGGAGATCGT 13021 GATACGTTCC CAGGGATGCG TTGAAGCGAT CCTCCACTCC GCTGATTTCA ACAGAGTCGA 13081 AACATTCAAT CTTTTTGAAA TAGTCTTCTT TGAGCTGTTT CACGGTAACT TTCCGGTTCG 13141 TCTTGAAGAG GAGGTCCACG ATAGCTTTCT TCTGCTCTCC AGACAGGAAT GCTGGCTTTC 13201 TCATCCCTTC TGTGACGTAT TTGACCTTGG TGAGCTCGTT ATAAACTGTG AAGTACTCGT 13261 ACAGCAGAGA GTGTTTAGGA AGCACCTTTT CGTTAGGCAG ATTTTTATCA AAGTTAGTCA 13321 TCCTTTCGAT GAAGGACTGG GCAGAGGCCC CCTTATCCAC GACTTCCTCG AAGTTCCAGG 13381 GAGTGATGGT CTCTTCTGAT TTGCGAGTCA TCCACGCGAA TCTGGAATTT CCCCGGGCGA 13441 GGGGGCCTAC ATAGTAGGGT ATCCGAAATG TGAGGATTTT CTCAATCTTT TCCCTGTTAT 13501 CTTTCAAAAA GGGGTAGAAA TCCTCTTGCC GCCTGAGGAT AGCGTGCAGT TCGCCCAGGT 13561 GAATCTGGTG GGGGATGCTT CCATTGTCGA AAGTGCGCTG TTTGCGCAAC AGATCTTCTC 13621 TGTTAAGCTT TACCAGCAGC TCCTCGGTGC CGTCCATTTT TTCCAAGATG GGCTTAATAA 13681 ATTTGTAAAA TTCCTCCTGG CTTGCTCCGC CGTCAATGTA TCCGGCGTAG CCATTTTTAG 13741 ACTGATCGAA GAAAATTTCC TTGTACTTCT CAGGCAGTTG CTGTCTGACA AGGGCCTTCA 13801 GCAAAGTCAA GTCTTGGTGG TGCTCATCAT AGCGCTTGAT CATACTAGCG CTCAGCGGAG 13861 CTTTGGTGAT CTCCGTGTTC ACTCGCAGAA TATCACTCAG CAGAATGGCG TCTGACAGGT 13921 TCTTTGCCGC CAAAAAAAGG TCTGCGTACT GGTCGCCGAT CTGGGCCAGC AGATTGTCGA 13981 GATCATCATC GTAGGTGTCT TTGCTCAGTT GAAGCTTGGC ATCTTCGGCC AGGTCGAAGT 14041 TAGATTTAAA GTTGGGGGTC AGCCCGAGTG ACAGGGCGAT AAGATTACCA AACAGGCCGT 14101 TCTTCTTCTC CCCAGGGAGC TGTGCGATGA GGTTTTCGAG CCGCCGGGAT TTGGACAGCC 14161 TAGCGCTCAG GATTGCTTTG GCGTCAACTC CGGATGCGTT GATCGGGTTC TCTTCGAAAA 14221 GCTGATTGTA AGTCTGAACC AGTTGGATAA AGAGTTTGTC GACATCGCTG TTGTCTGGGT 14281 TCAGGTCCCC CTCGATGAGG AAGTGTCCCC GAAATTTGAT CATATGCGCC AGCGCGAGAT 14341 AGATCAACCG CAAGTCAGCC TTATCAGTAC TGTCTACAAG CTTCTTCCTC AGATGATATA 14401 TGGTTGGGTA CTTTTCATGG TACGCCACCT CGTCCACGAT ATTGCCAAAG ATTGGGTGGC 14461 GCTCGTGCTT TTTATCCTCC TCCACCAAAA AGGACTCCTC CAGCCTATGG AAGAAAGAGT 14521 CATCCACCTT AGCCATCTCA TTACTAAAGA TCTCCTGCAG GTAGCAGATC CGATTCTTTC 14581 TGCGGGTATA TCTGCGCCGT GCTGTTCTTT TGAGCCGCGT GGCTTCGGCG GTTTCCCCGG 14641 AGTCGAACAG GAGGGCGCCA ATGAGGTTCT TCTTTATGCT GTGGCGATCG GTATTGCCCA 14701 GAACTTTGAA TTTTTTGCTC GGCACCTTGT ACTCGTCCGT AATGACGGCC CAGCCGACGC 14761 TGTTTGTGCC GATGGCGAGC CCAATGGAGT ACTTCTTGTC CATTCCGCGG CCCACCTTCC 14821 TCTTCTTCTT GGGGGCCATC TTGTCATCGT CATCCTTGTA ATCGATGTCA TGATCTTTAT 14881 AATCACCGTC ATGGTCTTTG TAGTC

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.