pLenti-U6-Negative-Control-sgR

pLenti-U6-Negative-Control-sgRNA1-Cas9-Puro plasmid

PVT107769

pLenti-U6-Negative-Control-sgRNA1-Cas9-Puro plasmid Information

Plasmid name: pLenti-U6-Negative-Control-sgRNA1-Cas9-Puro
Alias: CTAAGGTTAAGTCGCCCTC
Gene lenght:
Host: Mammalian cells,lentivirus
Use(s): Gene knockout,negative control
Fragment type: CRISPR
Species: Empty Vector
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: U6
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: U6-F(Hairpin structure)
Reverse primer:
Copy number: High
Induction:

pLenti-U6-Negative-Control-sgRNA1-Cas9-Puro plasmid Description

pLenti-U6-Negative-Control-sgRNA1-Cas9-Puro This plasmid is suitable for control and gene knockout in mammalian cells and can also be used for lentivirus packaging.

pLenti-U6-Negative-Control-sgRNA1-Cas9-Puro plasmid Sequence

1 TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA CGGGGATTTC 61 CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT CAACGGGACT 121 TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG CGTGTACGGT 181 GGGAGGTCTA TATAAGCAGC GCGTTTTGCC TGTACTGGGT CTCTCTGGTT AGACCAGATC 241 TGAGCCTGGG AGCTCTCTGG CTAACTAGGG AACCCACTGC TTAAGCCTCA ATAAAGCTTG 301 CCTTGAGTGC TTCAAGTAGT GTGTGCCCGT CTGTTGTGTG ACTCTGGTAA CTAGAGATCC 361 CTCAGACCCT TTTAGTCAGT GTGGAAAATC TCTAGCAGTG GCGCCCGAAC AGGGACTTGA 421 AAGCGAAAGG GAAACCAGAG GAGCTCTCTC GACGCAGGAC TCGGCTTGCT GAAGCGCGCA 481 CGGCAAGAGG CGAGGGGCGG CGACTGGTGA GTACGCCAAA AATTTTGACT AGCGGAGGCT 541 AGAAGGAGAG AGATGGGTGC GAGAGCGTCA GTATTAAGCG GGGGAGAATT AGATCGCGAT 601 GGGAAAAAAT TCGGTTAAGG CCAGGGGGAA AGAAAAAATA TAAATTAAAA CATATAGTAT 661 GGGCAAGCAG GGAGCTAGAA CGATTCGCAG TTAATCCTGG CCTGTTAGAA ACATCAGAAG 721 GCTGTAGACA AATACTGGGA CAGCTACAAC CATCCCTTCA GACAGGATCA GAAGAACTTA 781 GATCATTATA TAATACAGTA GCAACCCTCT ATTGTGTGCA TCAAAGGATA GAGATAAAAG 841 ACACCAAGGA AGCTTTAGAC AAGATAGAGG AAGAGCAAAA CAAAAGTAAG ACCACCGCAC 901 AGCAAGCGGC CGCTGATCTT CAGACCTGGA GGAGGAGATA TGAGGGACAA TTGGAGAAGT 961 GAATTATATA AATATAAAGT AGTAAAAATT GAACCATTAG GAGTAGCACC CACCAAGGCA 1021 AAGAGAAGAG TGGTGCAGAG AGAAAAAAGA GCAGTGGGAA TAGGAGCTTT GTTCCTTGGG 1081 TTCTTGGGAG CAGCAGGAAG CACTATGGGC GCAGCGTCAA TGACGCTGAC GGTACAGGCC 1141 AGACAATTAT TGTCTGGTAT AGTGCAGCAG CAGAACAATT TGCTGAGGGC TATTGAGGCG 1201 CAACAGCATC TGTTGCAACT CACAGTCTGG GGCATCAAGC AGCTCCAGGC AAGAATCCTG 1261 GCTGTGGAAA GATACCTAAA GGATCAACAG CTCCTGGGGA TTTGGGGTTG CTCTGGAAAA 1321 CTCATTTGCA CCACTGCTGT GCCTTGGAAT GCTAGTTGGA GTAATAAATC TCTGGAACAG 1381 ATTTGGAATC ACACGACCTG GATGGAGTGG GACAGAGAAA TTAACAATTA CACAAGCTTA 1441 ATACACTCCT TAATTGAAGA ATCGCAAAAC CAGCAAGAAA AGAATGAACA AGAATTATTG 1501 GAATTAGATA AATGGGCAAG TTTGTGGAAT TGGTTTAACA TAACAAATTG GCTGTGGTAT 1561 ATAAAATTAT TCATAATGAT AGTAGGAGGC TTGGTAGGTT TAAGAATAGT TTTTGCTGTA 1621 CTTTCTATAG TGAATAGAGT TAGGCAGGGA TATTCACCAT TATCGTTTCA GACCCACCTC 1681 CCAACCCCGA GGGGACCCGA CAGGCCCGAA GGAATAGAAG AAGAAGGTGG AGAGAGAGAC 1741 AGAGACAGAT CCATTCGATT AGTGAACGGA TCGGCACTGC GTGCGCCAAT TCTGCAGACA 1801 AATGGCAGTA TTCATCCACA ATTTTAAAAG AAAAGGGGGG ATTGGGGGGT ACAGTGCAGG 1861 GGAAAGAATA GTAGACATAA TAGCAACAGA CATACAAACT AAAGAATTAC AAAAACAAAT 1921 TACAAAAATT CAAAATTTTC GGGTTTATTA CAGGGACAGC AGAGATCCAG TTTGGTTAAT 1981 TAAGGTACCG AGGGCCTATT TCCCATGATT CCTTCATATT TGCATATACG ATACAAGGCT 2041 GTTAGAGAGA TAATTAGAAT TAATTTGACT GTAAACACAA AGATATTAGT ACAAAATACG 2101 TGACGTAGAA AGTAATAATT TCTTGGGTAG TTTGCAGTTT TAAAATTATG TTTTAAAATG 2161 GACTATCATA TGCTTACCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG 2221 TGGAAAGGAC GAAACACCGC TAAGGTTAAG TCGCCCTCGT TTTAGAGCTA GAAATAGCAA 2281 GTTAAAATAA GGCTAGTCCG TTATCAACTT GAAAAAGTGG CACCGAGTCG GTGCTTTTTT 2341 GAATTCGCTA GCTAGGTCTT GAAAGGAGTG GGAATTGGCT CCGGTGCCCG TCAGTGGGCA 2401 GAGCGCACAT CGCCCACAGT CCCCGAGAAG TTGGGGGGAG GGGTCGGCAA TTGATCCGGT 2461 GCCTAGAGAA GGTGGCGCGG GGTAAACTGG GAAAGTGATG TCGTGTACTG GCTCCGCCTT 2521 TTTCCCGAGG GTGGGGGAGA ACCGTATATA AGTGCAGTAG TCGCCGTGAA CGTTCTTTTT 2581 CGCAACGGGT TTGCCGCCAG AACACAGGAC CGGTTCTAGA GCGCTGCCAC CATGGACAAG 2641 AAGTACAGCA TCGGCCTGGA CATCGGCACC AACTCTGTGG GCTGGGCCGT GATCACCGAC 2701 GAGTACAAGG TGCCCAGCAA GAAATTCAAG GTGCTGGGCA ACACCGACCG GCACAGCATC 2761 AAGAAGAACC TGATCGGAGC CCTGCTGTTC GACAGCGGCG AAACAGCCGA GGCCACCCGG 2821 CTGAAGAGAA CCGCCAGAAG AAGATACACC AGACGGAAGA ACCGGATCTG CTATCTGCAA 2881 GAGATCTTCA GCAACGAGAT GGCCAAGGTG GACGACAGCT TCTTCCACAG ACTGGAAGAG 2941 TCCTTCCTGG TGGAAGAGGA TAAGAAGCAC GAGCGGCACC CCATCTTCGG CAACATCGTG 3001 GACGAGGTGG CCTACCACGA GAAGTACCCC ACCATCTACC ACCTGAGAAA GAAACTGGTG 3061 GACAGCACCG ACAAGGCCGA CCTGCGGCTG ATCTATCTGG CCCTGGCCCA CATGATCAAG 3121 TTCCGGGGCC ACTTCCTGAT CGAGGGCGAC CTGAACCCCG ACAACAGCGA CGTGGACAAG 3181 CTGTTCATCC AGCTGGTGCA GACCTACAAC CAGCTGTTCG AGGAAAACCC CATCAACGCC 3241 AGCGGCGTGG ACGCCAAGGC CATCCTGTCT GCCAGACTGA GCAAGAGCAG ACGGCTGGAA 3301 AATCTGATCG CCCAGCTGCC CGGCGAGAAG AAGAATGGCC TGTTCGGAAA CCTGATTGCC 3361 CTGAGCCTGG GCCTGACCCC CAACTTCAAG AGCAACTTCG ACCTGGCCGA GGATGCCAAA 3421 CTGCAGCTGA GCAAGGACAC CTACGACGAC GACCTGGACA ACCTGCTGGC CCAGATCGGC 3481 GACCAGTACG CCGACCTGTT TCTGGCCGCC AAGAACCTGT CCGACGCCAT CCTGCTGAGC 3541 GACATCCTGA GAGTGAACAC CGAGATCACC AAGGCCCCCC TGAGCGCCTC TATGATCAAG 3601 AGATACGACG AGCACCACCA GGACCTGACC CTGCTGAAAG CTCTCGTGCG GCAGCAGCTG 3661 CCTGAGAAGT ACAAAGAGAT TTTCTTCGAC CAGAGCAAGA ACGGCTACGC CGGCTACATT 3721 GACGGCGGAG CCAGCCAGGA AGAGTTCTAC AAGTTCATCA AGCCCATCCT GGAAAAGATG 3781 GACGGCACCG AGGAACTGCT CGTGAAGCTG AACAGAGAGG ACCTGCTGCG GAAGCAGCGG 3841 ACCTTCGACA ACGGCAGCAT CCCCCACCAG ATCCACCTGG GAGAGCTGCA CGCCATTCTG 3901 CGGCGGCAGG AAGATTTTTA CCCATTCCTG AAGGACAACC GGGAAAAGAT CGAGAAGATC 3961 CTGACCTTCC GCATCCCCTA CTACGTGGGC CCTCTGGCCA GGGGAAACAG CAGATTCGCC 4021 TGGATGACCA GAAAGAGCGA GGAAACCATC ACCCCCTGGA ACTTCGAGGA AGTGGTGGAC 4081 AAGGGCGCTT CCGCCCAGAG CTTCATCGAG CGGATGACCA ACTTCGATAA GAACCTGCCC 4141 AACGAGAAGG TGCTGCCCAA GCACAGCCTG CTGTACGAGT ACTTCACCGT GTATAACGAG 4201 CTGACCAAAG TGAAATACGT GACCGAGGGA ATGAGAAAGC CCGCCTTCCT GAGCGGCGAG 4261 CAGAAAAAGG CCATCGTGGA CCTGCTGTTC AAGACCAACC GGAAAGTGAC CGTGAAGCAG 4321 CTGAAAGAGG ACTACTTCAA GAAAATCGAG TGCTTCGACT CCGTGGAAAT CTCCGGCGTG 4381 GAAGATCGGT TCAACGCCTC CCTGGGCACA TACCACGATC TGCTGAAAAT TATCAAGGAC 4441 AAGGACTTCC TGGACAATGA GGAAAACGAG GACATTCTGG AAGATATCGT GCTGACCCTG 4501 ACACTGTTTG AGGACAGAGA GATGATCGAG GAACGGCTGA AAACCTATGC CCACCTGTTC 4561 GACGACAAAG TGATGAAGCA GCTGAAGCGG CGGAGATACA CCGGCTGGGG CAGGCTGAGC 4621 CGGAAGCTGA TCAACGGCAT CCGGGACAAG CAGTCCGGCA AGACAATCCT GGATTTCCTG 4681 AAGTCCGACG GCTTCGCCAA CAGAAACTTC ATGCAGCTGA TCCACGACGA CAGCCTGACC 4741 TTTAAAGAGG ACATCCAGAA AGCCCAGGTG TCCGGCCAGG GCGATAGCCT GCACGAGCAC 4801 ATTGCCAATC TGGCCGGCAG CCCCGCCATT AAGAAGGGCA TCCTGCAGAC AGTGAAGGTG 4861 GTGGACGAGC TCGTGAAAGT GATGGGCCGG CACAAGCCCG AGAACATCGT GATCGAAATG 4921 GCCAGAGAGA ACCAGACCAC CCAGAAGGGA CAGAAGAACA GCCGCGAGAG AATGAAGCGG 4981 ATCGAAGAGG GCATCAAAGA GCTGGGCAGC CAGATCCTGA AAGAACACCC CGTGGAAAAC 5041 ACCCAGCTGC AGAACGAGAA GCTGTACCTG TACTACCTGC AGAATGGGCG GGATATGTAC 5101 GTGGACCAGG AACTGGACAT CAACCGGCTG TCCGACTACG ATGTGGACCA TATCGTGCCT 5161 CAGAGCTTTC TGAAGGACGA CTCCATCGAC AACAAGGTGC TGACCAGAAG CGACAAGAAC 5221 CGGGGCAAGA GCGACAACGT GCCCTCCGAA GAGGTCGTGA AGAAGATGAA GAACTACTGG 5281 CGGCAGCTGC TGAACGCCAA GCTGATTACC CAGAGAAAGT TCGACAATCT GACCAAGGCC 5341 GAGAGAGGCG GCCTGAGCGA ACTGGATAAG GCCGGCTTCA TCAAGAGACA GCTGGTGGAA 5401 ACCCGGCAGA TCACAAAGCA CGTGGCACAG ATCCTGGACT CCCGGATGAA CACTAAGTAC 5461 GACGAGAATG ACAAGCTGAT CCGGGAAGTG AAAGTGATCA CCCTGAAGTC CAAGCTGGTG 5521 TCCGATTTCC GGAAGGATTT CCAGTTTTAC AAAGTGCGCG AGATCAACAA CTACCACCAC 5581 GCCCACGACG CCTACCTGAA CGCCGTCGTG GGAACCGCCC TGATCAAAAA GTACCCTAAG 5641 CTGGAAAGCG AGTTCGTGTA CGGCGACTAC AAGGTGTACG ACGTGCGGAA GATGATCGCC 5701 AAGAGCGAGC AGGAAATCGG CAAGGCTACC GCCAAGTACT TCTTCTACAG CAACATCATG 5761 AACTTTTTCA AGACCGAGAT TACCCTGGCC AACGGCGAGA TCCGGAAGCG GCCTCTGATC 5821 GAGACAAACG GCGAAACCGG GGAGATCGTG TGGGATAAGG GCCGGGATTT TGCCACCGTG 5881 CGGAAAGTGC TGAGCATGCC CCAAGTGAAT ATCGTGAAAA AGACCGAGGT GCAGACAGGC 5941 GGCTTCAGCA AAGAGTCTAT CCTGCCCAAG AGGAACAGCG ATAAGCTGAT CGCCAGAAAG 6001 AAGGACTGGG ACCCTAAGAA GTACGGCGGC TTCGACAGCC CCACCGTGGC CTATTCTGTG 6061 CTGGTGGTGG CCAAAGTGGA AAAGGGCAAG TCCAAGAAAC TGAAGAGTGT GAAAGAGCTG 6121 CTGGGGATCA CCATCATGGA AAGAAGCAGC TTCGAGAAGA ATCCCATCGA CTTTCTGGAA 6181 GCCAAGGGCT ACAAAGAAGT GAAAAAGGAC CTGATCATCA AGCTGCCTAA GTACTCCCTG 6241 TTCGAGCTGG AAAACGGCCG GAAGAGAATG CTGGCCTCTG CCGGCGAACT GCAGAAGGGA 6301 AACGAACTGG CCCTGCCCTC CAAATATGTG AACTTCCTGT ACCTGGCCAG CCACTATGAG 6361 AAGCTGAAGG GCTCCCCCGA GGATAATGAG CAGAAACAGC TGTTTGTGGA ACAGCACAAG 6421 CACTACCTGG ACGAGATCAT CGAGCAGATC AGCGAGTTCT CCAAGAGAGT GATCCTGGCC 6481 GACGCTAATC TGGACAAAGT GCTGTCCGCC TACAACAAGC ACCGGGATAA GCCCATCAGA 6541 GAGCAGGCCG AGAATATCAT CCACCTGTTT ACCCTGACCA ATCTGGGAGC CCCTGCCGCC 6601 TTCAAGTACT TTGACACCAC CATCGACCGG AAGAGGTACA CCAGCACCAA AGAGGTGCTG 6661 GACGCCACCC TGATCCACCA GAGCATCACC GGCCTGTACG AGACACGGAT CGACCTGTCT 6721 CAGCTGGGAG GCGACAAGCG ACCTGCCGCC ACAAAGAAGG CTGGACAGGC TAAGAAGAAG 6781 AAAGATTACA AAGACGATGA CGATAAGGGA TCCGGCGCAA CAAACTTCTC TCTGCTGAAA 6841 CAAGCCGGAG ATGTCGAAGA GAATCCTGGA CCGACCGAGT ACAAGCCCAC GGTGCGCCTC 6901 GCCACCCGCG ACGACGTCCC CAGGGCCGTA CGCACCCTCG CCGCCGCGTT CGCCGACTAC 6961 CCCGCCACGC GCCACACCGT CGATCCGGAC CGCCACATCG AGCGGGTCAC CGAGCTGCAA 7021 GAACTCTTCC TCACGCGCGT CGGGCTCGAC ATCGGCAAGG TGTGGGTCGC GGACGACGGC 7081 GCCGCGGTGG CGGTCTGGAC CACGCCGGAG AGCGTCGAAG CGGGGGCGGT GTTCGCCGAG 7141 ATCGGCCCGC GCATGGCCGA GTTGAGCGGT TCCCGGCTGG CCGCGCAGCA ACAGATGGAA 7201 GGCCTCCTGG CGCCGCACCG GCCCAAGGAG CCCGCGTGGT TCCTGGCCAC CGTCGGAGTC 7261 TCGCCCGACC ACCAGGGCAA GGGTCTGGGC AGCGCCGTCG TGCTCCCCGG AGTGGAGGCG 7321 GCCGAGCGCG CCGGGGTGCC CGCCTTCCTG GAGACCTCCG CGCCCCGCAA CCTCCCCTTC 7381 TACGAGCGGC TCGGCTTCAC CGTCACCGCC GACGTCGAGG TGCCCGAAGG ACCGCGCACC 7441 TGGTGCATGA CCCGCAAGCC CGGTGCCTGA ACGCGTTAAG TCGACAATCA ACCTCTGGAT 7501 TACAAAATTT GTGAAAGATT GACTGGTATT CTTAACTATG TTGCTCCTTT TACGCTATGT 7561 GGATACGCTG CTTTAATGCC TTTGTATCAT GCTATTGCTT CCCGTATGGC TTTCATTTTC 7621 TCCTCCTTGT ATAAATCCTG GTTGCTGTCT CTTTATGAGG AGTTGTGGCC CGTTGTCAGG 7681 CAACGTGGCG TGGTGTGCAC TGTGTTTGCT GACGCAACCC CCACTGGTTG GGGCATTGCC 7741 ACCACCTGTC AGCTCCTTTC CGGGACTTTC GCTTTCCCCC TCCCTATTGC CACGGCGGAA 7801 CTCATCGCCG CCTGCCTTGC CCGCTGCTGG ACAGGGGCTC GGCTGTTGGG CACTGACAAT 7861 TCCGTGGTGT TGTCGGGGAA ATCATCGTCC TTTCCTTGGC TGCTCGCCTG TGTTGCCACC 7921 TGGATTCTGC GCGGGACGTC CTTCTGCTAC GTCCCTTCGG CCCTCAATCC AGCGGACCTT 7981 CCTTCCCGCG GCCTGCTGCC GGCTCTGCGG CCTCTTCCGC GTCTTCGCCT TCGCCCTCAG 8041 ACGAGTCGGA TCTCCCTTTG GGCCGCCTCC CCGCGTCGAC TTTAAGACCA ATGACTTACA 8101 AGGCAGCTGT AGATCTTAGC CACTTTTTAA AAGAAAAGGG GGGACTGGAA GGGCTAATTC 8161 ACTCCCAACG AAGACAAGAT CTGCTTTTTG CTTGTACTGG GTCTCTCTGG TTAGACCAGA 8221 TCTGAGCCTG GGAGCTCTCT GGCTAACTAG GGAACCCACT GCTTAAGCCT CAATAAAGCT 8281 TGCCTTGAGT GCTTCAAGTA GTGTGTGCCC GTCTGTTGTG TGACTCTGGT AACTAGAGAT 8341 CCCTCAGACC CTTTTAGTCA GTGTGGAAAA TCTCTAGCAG GGCCCGTTTA AACCCGCTGA 8401 TCAGCCTCGA CTGTGCCTTC TAGTTGCCAG CCATCTGTTG TTTGCCCCTC CCCCGTGCCT 8461 TCCTTGACCC TGGAAGGTGC CACTCCCACT GTCCTTTCCT AATAAAATGA GGAAATTGCA 8521 TCGCATTGTC TGAGTAGGTG TCATTCTATT CTGGGGGGTG GGGTGGGGCA GGACAGCAAG 8581 GGGGAGGATT GGGAAGACAA TAGCAGGCAT GCTGGGGATG CGGTGGGCTC TATGGCTTCT 8641 GAGGCGGAAA GAACCAGCTG GGGCTCTAGG GGGTATCCCC ACGCGCCCTG TAGCGGCGCA 8701 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 8761 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 8821 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 8881 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 8941 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 9001 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 9061 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTA ATTCTGTGGA 9121 ATGTGTGTCA GTTAGGGTGT GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA 9181 GCATGCATCT CAATTAGTCA GCAACCAGGT GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA 9241 GAAGTATGCA AAGCATGCAT CTCAATTAGT CAGCAACCAT AGTCCCGCCC CTAACTCCGC 9301 CCATCCCGCC CCTAACTCCG CCCAGTTCCG CCCATTCTCC GCCCCATGGC TGACTAATTT 9361 TTTTTATTTA TGCAGAGGCC GAGGCCGCCT CTGCCTCTGA GCTATTCCAG AAGTAGTGAG 9421 GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA AAAAGCTCCC GGGAGCTTGT ATATCCATTT 9481 TCGGATCTGA TCAGCACGTG TTGACAATTA ATCATCGGCA TAGTATATCG GCATAGTATA 9541 ATACGACAAG GTGAGGAACT AAACCATGGC CAAGTTGACC AGTGCCGTTC CGGTGCTCAC 9601 CGCGCGCGAC GTCGCCGGAG CGGTCGAGTT CTGGACCGAC CGGCTCGGGT TCTCCCGGGA 9661 CTTCGTGGAG GACGACTTCG CCGGTGTGGT CCGGGACGAC GTGACCCTGT TCATCAGCGC 9721 GGTCCAGGAC CAGGTGGTGC CGGACAACAC CCTGGCCTGG GTGTGGGTGC GCGGCCTGGA 9781 CGAGCTGTAC GCCGAGTGGT CGGAGGTCGT GTCCACGAAC TTCCGGGACG CCTCCGGGCC 9841 GGCCATGACC GAGATCGGCG AGCAGCCGTG GGGGCGGGAG TTCGCCCTGC GCGACCCGGC 9901 CGGCAACTGC GTGCACTTCG TGGCCGAGGA GCAGGACTGA CACGTGCTAC GAGATTTCGA 9961 TTCCACCGCC GCCTTCTATG AAAGGTTGGG CTTCGGAATC GTTTTCCGGG ACGCCGGCTG 10021 GATGATCCTC CAGCGCGGGG ATCTCATGCT GGAGTTCTTC GCCCACCCCA ACTTGTTTAT 10081 TGCAGCTTAT AATGGTTACA AATAAAGCAA TAGCATCACA AATTTCACAA ATAAAGCATT 10141 TTTTTCACTG CATTCTAGTT GTGGTTTGTC CAAACTCATC AATGTATCTT ATCATGTCTG 10201 TATACCGTCG ACCTCTAGCT AGAGCTTGGC GTAATCATGG TCATAGCTGT TTCCTGTGTG 10261 AAATTGTTAT CCGCTCACAA TTCCACACAA CATACGAGCC GGAAGCATAA AGTGTAAAGC 10321 CTGGGGTGCC TAATGAGTGA GCTAACTCAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT 10381 CCAGTCGGGA AACCTGTCGT GCCAGCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG 10441 CGGTTTGCGT ATTGGGCGCT CTTCCGCTTC CTCGCTCACT GACTCGCTGC GCTCGGTCGT 10501 TCGGCTGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 10561 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 10621 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 10681 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 10741 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 10801 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 10861 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 10921 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 10981 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 11041 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 11101 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 11161 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 11221 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 11281 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 11341 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG 11401 TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT 11461 AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC 11521 CAGTGCTGCA ATGATACCGC GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA 11581 CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA 11641 GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA 11701 CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT 11761 CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC 11821 GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT 11881 CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC 11941 TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG 12001 CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT 12061 CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC 12121 CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG 12181 CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC 12241 ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG 12301 TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT 12361 TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGTCGAC GGATCGGGAG ATCTCCCGAT 12421 CCCCTATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATCTG 12481 CTCCCTGCTT GTGTGTTGGA GGTCGCTGAG TAGTGCGCGA GCAAAATTTA AGCTACAACA 12541 AGGCAAGGCT TGACCGACAA TTGCATGAAG AATCTGCTTA GGGTTAGGCG TTTTGCGCTG 12601 CTTCGCGATG TACGGGCCAG ATATACGCGT TGACATTGAT TATTGACTAG TTATTAATAG 12661 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT 12721 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG 12781 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT 12841 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT 12901 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG 12961 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.