PiggyBac-EF1a-H1-4-EGFP-EF1a-P

PiggyBac-EF1a-H1-4-EGFP-EF1a-Puro plasmid

PVT107718

PiggyBac-EF1a-H1-4-EGFP-EF1a-Puro plasmid Information

Plasmid name: PiggyBac-EF1a-H1-4(human)-EGFP-EF1a-Puro
Alias: NM_005321.3;dJ221C16.5; H1.4; H1E; H1F4; H1s-4;HIST1H1E; RMNS
Gene lenght: 657
Host: Mammalian cells
Use(s): Gene expression,Transposition
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: EF-1α
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: EF-1α promoter-F1
Reverse primer:
Copy number: High
Induction:

PiggyBac-EF1a-H1-4-EGFP-EF1a-Puro plasmid Description

PiggyBac-EF1a-H1-4-EGFP-EF1a-Puro is a plasmid suitable for protein expression and transposition in mammalian cells.

PiggyBac-EF1a-H1-4-EGFP-EF1a-Puro plasmid Sequence

1 ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA 61 CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA 121 AGTGCCACCT AAATTGTAAG CGTTAATATT TTGTTAAAAT TCGCGTTAAA TTTTTGTTAA 181 ATCAGCTCAT TTTTTAACCA ATAGGCCGAA ATCGGCAAAA TCCCTTATAA ATCAAAAGAA 241 TAGACCGAGA TAGGGTTGAG TGTTGTTCCA GTTTGGAACA AGAGTCCACT ATTAAAGAAC 301 GTGGACTCCA ACGTCAAAGG GCGAAAAACC GTCTATCAGG GCGATGGCCC ACTACGTGAA 361 CCATCACCCT AATCAAGTTT TTTGGGGTCG AGGTGCCGTA AAGCACTAAA TCGGAACCCT 421 AAAGGGAGCC CCCGATTTAG AGCTTGACGG GGAAAGCCGG CGAACGTGGC GAGAAAGGAA 481 GGGAAGAAAG CGAAAGGAGC GGGCGCTAGG GCGCTGGCAA GTGTAGCGGT CACGCTGCGC 541 GTAACCACCA CACCCGCCGC GCTTAATGCG CCGCTACAGG GCGCGTCCCA TTCGCCATTC 601 AGGCTGCGCA ACTGTTGGGA AGGGCGATCG GTGCGGGCCT CTTCGCTATT ACGCCAGCTG 661 GCGAAAGGGG GATGTGCTGC AAGGCGATTA AGTTGGGTAA CGCCAGGGTT TTCCCAGTCA 721 CGACGTTGTA AAACGACGGC CAGTGAGCGC GCCTCGTTCA TTCACGTTTT TGAACCCGTG 781 GAGGACGGGC AGACTCGCGG TGCAAATGTG TTTTACAGCG TGATGGAGCA GATGAAGATG 841 CTCGACACGC TGCAGAACAC GCAGCTAGAT TAACCCTAGA AAGATAATCA TATTGTGACG 901 TACGTTAAAG ATAATCATGC GTAAAATTGA CGCATGTGTT TTATCGGTCT GTATATCGAG 961 GTTTATTTAT TAATTTGAAT AGATATTAAG TTTTATTATA TTTACACTTA CATACTAATA 1021 ATAAATTCAA CAAACAATTT ATTTATGTTT ATTTATTTAT TAAAAAAAAA CAAAAACTCA 1081 AAATTTCTTC TATAAAGTAA CAAAACTTTT ATGAGGGACA GCCCCCCCCC AAAGCCCCCA 1141 GGGATGTAAT TACGTCCCTC CCCCGCTAGG GGGCAGCAGC GAGCCGCCCG GGGCTCCGCT 1201 CCGGTCCGGC GCTCCCCCCG CATCCCCGAG CCGGCAGCGT GCGGGGACAG CCCGGGCACG 1261 GGGAAGGTGG CACGGGATCG CTTTCCTCTG AACGCTTCTC GCTGCTCTTT GAGCCTGCAG 1321 ACACCTGGGG GGATACGGGG AAAAGGCCTC CACGGCCACT AGTATTATGC CCAGTACATG 1381 ACCTTATGGG ACTTTCCTAC TTGGCAGTAC ATCTACGTAT TAGTCATCGC TATTACCATG 1441 GCTCCGGTGC CCGTCAGTGG GCAGAGCGCA CATCGCCCAC AGTCCCCGAG AAGTTGGGGG 1501 GAGGGGTCGG CAATTGAACC GGTGCCTAGA GAAGGTGGCG CGGGGTAAAC TGGGAAAGTG 1561 ATGTCGTGTA CTGGCTCCGC CTTTTTCCCG AGGGTGGGGG AGAACCGTAT ATAAGTGCAG 1621 TAGTCGCCGT GAACGTTCTT TTTCGCAACG GGTTTGCCGC CAGAACACAG GTAAGTGCCG 1681 TGTGTGGTTC CCGCGGGCCT GGCCTCTTTA CGGGTTATGG CCCTTGCGTG CCTTGAATTA 1741 CTTCCACCTG GCTGCAGTAC GTGATTCTTG ATCCCGAGCT TCGGGTTGGA AGTGGGTGGG 1801 AGAGTTCGAG GCCTTGCGCT TAAGGAGCCC CTTCGCCTCG TGCTTGAGTT GAGGCCTGGC 1861 CTGGGCGCTG GGGCCGCCGC GTGCGAATCT GGTGGCACCT TCGCGCCTGT CTCGCTGCTT 1921 TCGATAAGTC TCTAGCCATT TAAAATTTTT GATGACCTGC TGCGACGCTT TTTTTCTGGC 1981 AAGATAGTCT TGTAAATGCG GGCCAAGATC TGCACACTGG TATTTCGGTT TTTGGGGCCG 2041 CGGGCGGCGA CGGGGCCCGT GCGTCCCAGC GCACATGTTC GGCGAGGCGG GGCCTGCGAG 2101 CGCGGCCACC GAGAATCGGA CGGGGGTAGT CTCAAGCTGG CCGGCCTGCT CTGGTGCCTG 2161 GCCTCGCGCC GCCGTGTATC GCCCCGCCCT GGGCGGCAAG GCTGGCCCGG TCGGCACCAG 2221 TTGCGTGAGC GGAAAGATGG CCGCTTCCCG GCCCTGCTGC AGGGAGCTCA AAATGGAGGA 2281 CGCGGCGCTC GGGAGAGCGG GCGGGTGAGT CACCCACACA AAGGAAAAGG GCCTTTCCGT 2341 CCTCAGCCGT CGCTTCATGT GACTCCACGG AGTACCGGGC GCCGTCCAGG CACCTCGATT 2401 AGTTCTCGAG CTTTTGGAGT ACGTCGTCTT TAGGTTGGGG GGAGGGGTTT TATGCGATGG 2461 AGTTTCCCCA CACTGAGTGG GTGGAGACTG AAGTTAGGCC AGCTTGGCAC TTGATGTAAT 2521 TCTCCTTGGA ATTTGCCCTT TTTGAGTTTG GATCTTGGTT CATTCTCAAG CCTCAGACAG 2581 TGGTTCAAAG TTTTTTTCTT CCATTTCAGG TGTCGTGAGG AATTAGCTTG GTACGCTAGC 2641 GTTTAAACTT AAGCTTGGTA CCGAATTCgc caccatgtcc gagactgcgc ctgccgcgcc 2701 cgctgctccg gcccctgccg agaagactcc cgtgaagaag aaggcccgca agtctgcagg 2761 tgcggccaag cgcaaagcgt ctgggccccc ggtgtccgag ctcattacta aagctgttgc 2821 cgcctccaag gagcgcagcg gcgtatcttt ggccgctctc aagaaagcgc tggcagccgc 2881 tggctatgac gtggagaaga acaacagccg catcaagctg ggtctcaaga gcctggtgag 2941 caagggcacc ctggtgcaga ccaagggcac cggcgcgtcg ggttccttca aactcaacaa 3001 gaaggcggcc tctggggaag ccaagcctaa ggctaaaaag gcaggcgcgg ccaaggccaa 3061 gaagccagca ggagcggcga agaagcccaa gaaggcgacg ggggcggcca cccccaagaa 3121 gagcgccaag aagaccccaa agaaggcgaa gaagccggct gcagctgctg gagccaaaaa 3181 agcgaaaagc ccgaaaaagg cgaaagcagc caagccaaaa aaggcgccca agagcccagc 3241 gaaggccaaa gcagttaaac ccaaggcggc taaaccaaag accgccaagc ccaaggcagc 3301 caagccaaag aaggcggcag ccaagaaaaa gGGCGGTGGA GGAAGCggcg gaggtggatc 3361 tatggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga 3421 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta 3481 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac 3541 cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa 3601 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt 3661 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct 3721 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca 3781 caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa 3841 cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc 3901 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca 3961 ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt 4021 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta 4081 gGGATCCGCG GCCGCGAAGG ATCTGCGATC GCTCCGGTGC CCGTCAGTGG GCAGAGCGCA 4141 CATCGCCCAC AGTCCCCGAG AAGTTGGGGG GAGGGGTCGG CAATTGAACG GGTGCCTAGA 4201 GAAGGTGGCG CGGGGTAAAC TGGGAAAGTG ATGTCGTGTA CTGGCTCCGC CTTTTTCCCG 4261 AGGGTGGGGG AGAACCGTAT ATAAGTGCAG TAGTCGCCGT GAACGTTCTT TTTCGCAACG 4321 GGTTTGCCGC CAGAACACAG CTGAAGCTTC GAGGGGCTCG CATCTCTCCT TCACGCGCCC 4381 GCCGCCCTAC CTGAGGCCGC CATCCACGCC GGTTGAGTCG CGTTCTGCCG CCTCCCGCCT 4441 GTGGTGCCTC CTGAACTGCG TCCGCCGTCT AGGTAAGTTT AAAGCTCAGG TCGAGACCGG 4501 GCCTTTGTCC GGCGCTCCCT TGGAGCCTAC CTAGACTCAG CCGGCTCTCC ACGCTTTGCC 4561 TGACCCTGCT TGCTCAACTC TACGTCTTTG TTTCGTTTTC TGTTCTGCGC CGTTACAGAT 4621 CCAAGCTGTG ACCGGCGCCT ACGCTAGACG CCACCATGAC CGAGTACAAG CCCACGGTGC 4681 GCCTCGCCAC CCGCGACGAC GTCCCCAGGG CCGTACGCAC CCTCGCCGCC GCGTTCGCCG 4741 ACTACCCCGC CACGCGCCAC ACCGTCGATC CGGACCGCCA CATCGAGCGG GTCACCGAGC 4801 TGCAAGAACT CTTCCTCACG CGCGTCGGGC TCGACATCGG CAAGGTGTGG GTCGCGGACG 4861 ACGGCGCCGC GGTGGCGGTC TGGACCACGC CGGAGAGCGT CGAAGCGGGG GCGGTGTTCG 4921 CCGAGATCGG CCCGCGCATG GCCGAGTTGA GCGGTTCCCG GCTGGCCGCG CAGCAACAGA 4981 TGGAAGGCCT CCTGGCGCCG CACCGGCCCA AGGAGCCCGC GTGGTTCCTG GCCACCGTCG 5041 GCGTCTCGCC CGACCACCAG GGCAAGGGTC TGGGCAGCGC CGTCGTGCTC CCCGGAGTGG 5101 AGGCGGCCGA GCGCGCCGGG GTGCCCGCCT TCCTGGAGAC CTCCGCGCCC CGCAACCTCC 5161 CCTTCTACGA GCGGCTCGGC TTCACCGTCA CCGCCGACGT CGAGGTGCCC GAAGGACCGC 5221 GCACCTGGTG CATGACCCGC AAGCCCGGTG CCTGAAATCA ACCTCTGGAT TACAAAATTT 5281 GTGAAAGATT GACTGGTATT CTTAACTATG TTGCTCCTTT TACGCTATGT GGATACGCTG 5341 CTTTAATGCC TTTGTATCAG TTAACTTGTT TATTGCAGCT TATAATGGTT ACAAATAAAG 5401 CAATAGCATC ACAAATTTCA CAAATAAAGC ATTTTTTTCA CTGCATTCTA GTTGTGGTTT 5461 GTCCAAACTC ATCAATGTAT CTTATCATGT CTGGAATTGA CTCAAATGAT GTCAATTAGT 5521 CTATCAGAAG CTATCTGGTC TCCCTTCCGG GGGACAAGAC ATCCCTGTTT AATATTTAAA 5581 CAGCAGTGTT CCCAAACTGG GTTCTTATAT CCCTTGCTCT GGTCAACCAG GTTGCAGGGT 5641 TTCCTGTCCT CACAGGAACG AAGTCCCTAA AGAAACAGTG GCAGCCAGGT TTAGCCCCGG 5701 AATTGACTGG ATTCCTTTTT TAGGGCCCAT TGGTATGGCT TTTTCCCCGT ATCCCCCCAG 5761 GTGTCTGCAG GCTCAAAGAG CAGCGAGAAG CGTTCAGAGG AAAGCGATCC CGTGCCACCT 5821 TCCCCGTGCC CGGGCTGTCC CCGCACGCTG CCGGCTCGGG GATGCGGGGG GAGCGCCGGA 5881 CCGGAGCGGA GCCCCGGGCG GCTCGCTGCT GCCCCCTAGC GGGGGAGGGA CGTAATTACA 5941 TCCCTGGGGG CTTTGGGGGG GGGCTGTCCC TGATATCTAT AACAAGAAAA TATATATATA 6001 ATAAGTTATC ACGTAAGTAG AACATGAAAT AACAATATAA TTATCGTATG AGTTAAATCT 6061 TAAAAGTCAC GTAAAAGATA ATCATGCGTC ATTTTGACTC ACGCGGTCGT TATAGTTCAA 6121 AATCAGTGAC ACTTACCGCA TTGACAAGCA CGCCTCACGG GAGCTCCAAG CGGCGACTGA 6181 GATGTCCTAA ATGCACAGCG ACGGATTCGC GCTATTTAGA AAGAGAGAGC AATATTTCAA 6241 GAATGCATGC GTCAATTTTA CGCAGACTAT CTTTCTAGGG TTAATCTAGC TGCATCAGGA 6301 TCATATCGTC GGGTCTTTTT TCCGGCTCAG TCATCGCCCA AGCTGGCGCT ATCTGGGCAT 6361 CGGGGAGGAA GAAGCCCGTG CCTTTTCCCG CGAGGTTGAA GCGGCATGGA AAGAGTTTGC 6421 CGAGGATGAC TGCTGCTGCA TTGACGTTGA GCGAAAACGC ACGTTTACCA TGATGATTCG 6481 GGAAGGTGTG GCCATGCACG CCTTTAACGG TGAACTGTTC GTTCAGGCCA CCTGGGATAC 6541 CAGTTCGTCG CGGCTTTTCC GGACACAGTT CCGGATGGTC AGCCCGAAGC GCATCAGCAA 6601 CCCGAACAAT ACCGGCGACA GCCGGAACTG CCGTGCCGGT GTGCAGATTA ATGACAGCGG 6661 TGCGGCGCTG GGATATTACG TCAGCGAGGA CGGGTATCCT GGCTGGATGC CGCAGAAATG 6721 GACATGGATA CCCCGTGAGT TACCCGGCGG GCGCGCTTGG CGTAATCATG GTCATAGCTG 6781 TTTCCTGTGT GAAATTGTTA TCCGCTCACA ATTCCACACA ACATACGAGC CGGAAGCATA 6841 AAGTGTAAAG CCTGGGGTGC CTAATGAGTG AGCTAACTCA CATTAATTGC GTTGCGCTCA 6901 CTGCCCGCTT TCCAGTCGGG AAACCTGTCG TGCCAGCTGC ATTAATGAAT CGGCCAACGC 6961 GCGGGGAGAG GCGGTTTGCG TATTGGGCGC TCTTCCGCTT CCTCGCTCAC TGACTCGCTG 7021 CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA 7081 TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC 7141 AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG 7201 CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC 7261 CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC 7321 GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT 7381 AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC 7441 GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA 7501 CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA 7561 GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG AAGGACAGTA 7621 TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA 7681 TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG 7741 CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG 7801 TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC 7861 TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT 7921 TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT 7981 CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA 8041 CCATCTGGCC CCAGTGCTGC AATGATACCG CGAGACCCAC GCTCACCGGC TCCAGATTTA 8101 TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC 8161 GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT 8221 AGTTTGCGCA ACGTTGTTGC CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT 8281 ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG 8341 TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA 8401 GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA 8461 AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG 8521 CGACCGAGTT GCTCTTGCCC GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT 8581 TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG 8641 CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT 8701 ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA 8761 ATAAGGGCGA CACGGAAATG TTGAATACTC AT

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.