pLV3-CMV-Mark2-3×HA-EF1a-Fluc-

pLV3-CMV-Mark2-3×HA-EF1a-Fluc-Puro plasmid

PVT107450

pLV3-CMV-Mark2-3×HA-EF1a-Fluc-Puro plasmid Information

Plasmid name: pLV3-CMV-Mark2(mouse)-3×HA-EF1a-Fluc-Puro
Alias: NM_007928.4;Emk; EMK-1; Par-1; Par-1b;
Gene lenght: 2328
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Fluc
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-Mark2-3×HA-EF1a-Fluc-Puro plasmid Description

pLV3-CMV-Mark2-3×HA-EF1a-Fluc-Puro It is a plasmid suitable for protein expression in mammalian cells and is also applicable for lentivirus packaging.

pLV3-CMV-Mark2-3×HA-EF1a-Fluc-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt 1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc 1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga 1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac 1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga 2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg 2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg 2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac 2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagagc 2281 tagcgaattc GCCACCatgt ccagcgctcg gaccccccta cccacgctga acgaaaggga 2341 cacggagcag cccaccttgg gacaccttga ttccaagccc agcagtaagt ccaacatgct 2401 gcggggccgc aactcagcca cctctgctga cgagcagccc catattggca actaccggct 2461 ccttaagacc attggcaagg gtaactttgc caaggtgaag ttggcccggc acatcctgac 2521 ggggaaagag gtagctgtga agatcatcga caagacccag ctgaactcct ccagcctaca 2581 gaaactgttc cgagaagtaa gaataatgaa ggttttgaat catcccaaca tagttaagtt 2641 gtttgaagtg atcgagactg agaagactct ctaccttgtc atggagtatg ccagtggcgg 2701 agaggtgttt gattacctag tggcccatgg caggatgaaa gaaaaagaag ctcgagccaa 2761 atttcgccag atagtgtctg ctgtgcagta ctgtcaccag aagttcattg ttcatagaga 2821 tctaaaggca gaaaacctgc tcctggatgc tgatatgaac atcaagattg cagactttgg 2881 ctttagcaac gaattcacct ttgggaacaa gctggatact ttctgtggca gtcctcctta 2941 tgctgcccca gaacttttcc agggcaaaaa gtatgatggt cctgaggtgg atgtctggag 3001 cctgggagtc atcctctata cactggtcag cggatccctg ccttttgatg gacagaacct 3061 caaggagctg cgggaacggg tactgagggg gaaataccgt attccgttct acatgtccac 3121 ggactgtgaa aatctgctta agaaatttct catacttaat cctagtaaga gaggcacttt 3181 agagcaaatt atgaaagatc ggtggatgaa cgtggggcat gaggacgatg agctaaagcc 3241 ttatgtggaa cctctccctg actacaagga cccccggcgg acagagttga tggtgtcaat 3301 gggttacaca cgggaagaga tccaggactc gctggtaggc cagaggtaca acgaagtgat 3361 ggctacctat ctgctccttg gctacaagag ctctgagctg gaaggtgata ccatcacttt 3421 gaagccccgg ccttcagctg atctaaccaa cagcagtgcc ccatctccat cccacaaggt 3481 tcagcgcagc gtctctgcca accccaagca acgacgctcc agtgaccagg ccgtccctgc 3541 cattcccacc tcgaattcct actctaagaa gactcagagt aacaacgcag aaaataagcg 3601 gcctgaggaa gagacagggc ggaaagccag cagcaccgcc aaagtgcctg ccagccctct 3661 gcctggcctg gacaggaaga agaccactcc tgccccctcc acgaacagcg tcctttccac 3721 cagcacaaac cgaagcagga actccccact tttggacagg gccagccttg gccaggcctc 3781 catccagaat ggtaaagaca gcctaaccat gccagggtcc cgggcctcca cggcttctgc 3841 ttctgccgca gtctctgcgg cccggccccg ccagcaccag aaatccatgt ctgcctccgt 3901 acaccccaac aaggcctctg ggttgccccc cacggagagt aactgtgagg tgcctcggcc 3961 cagcacagcc ccccagcgcg tccctgtcgc ctccccctcc gcccacaaca tcagcagcag 4021 tagtggagcc ccagaccgaa ctaatttccc acggggtgtg tccagtcgaa gcaccttcca 4081 tgctgggcag ctccgacagg tgcgggacca gcagaatcta ccctacggtg tgaccccagc 4141 ctctccctct ggccatagcc agggccggcg gggggcctct ggcagcatct tcagcaagtt 4201 cacctccaag tttgtccgca ggaacctgaa tgaacctgaa agcaaagacc gagtggagac 4261 gctcagacct cacgtggtag gcagtggagg cactgacaag gacaaggagg agtttcggga 4321 ggccaagcct cgctccctgc gcttcacctg gagcatgaag accacgagct ctatggagcc 4381 caatgagatg atgcgggaga tccgcaaggt gctggacgcc aacagctgcc aaagcgagct 4441 gcacgagcgg tacatgctac tgtgcgtgca tggcacacca ggccacgaga actttgtgca 4501 gtgggagatg gaggtgtgca aactgccccg gctgtctctc aacggtgttc ggtttaagcg 4561 gatatcgggc acttccatgg ccttcaaaaa cattgcctcc aaaatagcca atgagctgaa 4621 gcttggatcc atgTACCCAT ACGACGTACC TGATTATGCA ACTTATCCTT ATGATGTACC 4681 TGATTATGCT AGCTATCCTT ATGATGTCCC AGACTACGCA tagggatccG ATATCCTCGA 4741 GTCTGGGCCC gcggccgcaa ggatctgcga tcgctccggt gcccgtcagt gggcagagcg 4801 cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa cgggtgccta 4861 gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc gcctttttcc 4921 cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa 4981 cgggtttgcc gccagaacac agctgaagct tcgaggggct cgcatctctc cttcacgcgc 5041 ccgccgccct acctgaggcc gccatccacg ccggttgagt cgcgttctgc cgcctcccgc 5101 ctgtggtgcc tcctgaactg cgtccgccgt ctaggtaagt ttaaagctca ggtcgagacc 5161 gggcctttgt ccggcgctcc cttggagcct acctagactc agccggctct ccacgctttg 5221 cctgaccctg cttgctcaac tctacgtctt tgtttcgttt tctgttctgc gccgttacag 5281 atccaagctg tgaccggcgc ctacgctaga cgccaccatg gagagcgacg agagcggcct 5341 gatggaagac gccaaaaaca taaagaaagg cccggcgcca ttctatccgc tggaagatgg 5401 aaccgctgga gagcaactgc ataaggctat gaagagatac gccctggttc ctggaacaat 5461 tgcttttaca gatgcacata tcgaggtgga catcacttac gctgagtact tcgaaatgtc 5521 cgttcggttg gcagaagcta tgaaacgata tgggctgaat acaaatcaca gaatcgtcgt 5581 atgcagtgaa aactctcttc aattctttat gccggtgttg ggcgcgttat ttatcggagt 5641 tgcagttgcg cccgcgaacg acatttataa tgaacgtgaa ttgctcaaca gtatgggcat 5701 ttcgcagcct accgtggtgt tcgtttccaa aaaggggttg caaaaaattt tgaacgtgca 5761 aaaaaagctc ccaatcatcc aaaaaattat tatcatggat tctaaaacgg attaccaggg 5821 atttcagtcg atgtacacgt tcgtcacatc tcatctacct cccggtttta atgaatacga 5881 ttttgtgcca gagtccttcg atagggacaa gacaattgca ctgatcatga actcctctgg 5941 atctactggt ctgcctaaag gtgtcgctct gcctcataga actgcctgcg tgagattctc 6001 gcatgccaga gatcctattt ttggcaatca aatcattccg gatactgcga ttttaagtgt 6061 tgttccattc catcacggtt ttggaatgtt tactacactc ggatatttga tatgtggatt 6121 tcgagtcgtc ttaatgtata gatttgaaga agagctgttt ctgaggagcc ttcaggatta 6181 caagattcaa agtgcgctgc tggtgccaac cctattctcc ttcttcgcca aaagcactct 6241 gattgacaaa tacgatttat ctaatttaca cgaaattgct tctggtggcg ctcccctctc 6301 taaggaagtc ggggaagcgg ttgccaagag gttccatctg ccaggtatca ggcaaggata 6361 tgggctcact gagactacat cagctattct gattacaccc gagggggatg ataaaccggg 6421 cgcggtcggt aaagttgttc cattttttga agcgaaggtt gtggatctgg ataccgggaa 6481 aacgctgggc gttaatcaaa gaggcgaact gtgtgtgaga ggtcctatga ttatgtccgg 6541 ttatgtaaac aatccggaag cgaccaacgc cttgattgac aaggatggat ggctacattc 6601 tggagacata gcttactggg acgaagacga acacttcttc atcgttgacc gcctgaagtc 6661 tctgattaag tacaaaggct atcaggtggc tcccgctgaa ttggaatcca tcttgctcca 6721 acaccccaac atcttcgacg caggtgtcgc aggtcttccc gacgatgacg ccggtgaact 6781 tcccgccgcc gttgttgttt tggagcacgg aaagacgatg acggaaaaag agatcgtgga 6841 ttacgtcgcc agtcaagtaa caaccgcgaa aaagttgcgc ggaggagttg tgtttgtgga 6901 cgaagtaccg aaaggtctta ccggaaaact cgacgcaaga aaaatcagag agatcctcat 6961 aaaggccaag aagggcggaa agatcgccgt gagatcccgc gctcagtcgt ccaattctgc 7021 cgtggacggc accgccggac ccggctccac cggatctcgc gagggcagag gaagtcttct 7081 aacatgcggt gacgtggagg agaatcccgg ccctatgacc gagtacaagc ccacggtgcg 7141 cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga 7201 ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct 7261 gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga 7321 cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc 7381 cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat 7441 ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg 7501 cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga 7561 ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc 7621 cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg 7681 cacctggtgc atgacccgca agcccggtgc ctgaaatcaa cctctggatt acaaaatttg 7741 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc 7801 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta 7861 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt 7921 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca 7981 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc 8041 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt 8101 gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg 8161 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg 8221 cctgctgccg gctctgcggc ctcttccgcg tctccgcctt cgccctcaga cgagtcggat 8281 ctccctttgg ccgcctcccc gcctggtacc tttaagacca atgacttaca aggcagctgt 8341 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg 8401 aaaataagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg 8461 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt 8521 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc 8581 cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta 8641 tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta 8701 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact 8761 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct 8821 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt 8881 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga 8941 ggcttttttg gaggcctaga cttttgcaga gacggcccaa attcgtaatc atggtcatag 9001 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc 9061 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc 9121 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa 9181 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg 9241 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg 9301 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag 9361 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac 9421 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga 9481 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt 9541 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc 9601 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc 9661 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta 9721 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat 9781 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca 9841 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct 9901 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt 9961 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct 10021 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc 10081 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa 10141 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta 10201 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc 10261 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat 10321 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta 10381 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt 10441 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt 10501 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg 10561 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc 10621 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc 10681 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg 10741 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga 10801 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta 10861 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct 10921 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag 10981 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga 11041 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat 11101 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc 11161 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg 11221 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct 11281 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc 11341 gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat 11401 atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gcgccattcg 11461 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc 11521 cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc 11581 cagtcacgac gttgtaaaac gacggccagt gccaagctg

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.