pLV3-CMV-mir194-EF1a-CopGFP-Pu

pLV3-CMV-mir194-EF1a-CopGFP-Puro plasmid

PVT107391

pLV3-CMV-mir194-EF1a-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-mir194(dog)-EF1a-CopGFP-Puro
Alias:
Gene lenght: 2001
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Canine
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-mir194-EF1a-CopGFP-Puro plasmid Description

pLV3-CMV-mir194-EF1a-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and is also applicable for lentivirus packaging.

pLV3-CMV-mir194-EF1a-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc ATATAAAGTA TAGATAACAT TTACTTCAAA 2341 TTGAAATTAA ACATCAGAGT TTCAAATCTA CCAGTCTCTA AGCCTATTTA GAATGTGAAT 2401 AAATCAATAT TTTCTGTCGT GCAACACGTA TAATTAAGGC TTTAACTTAC AGACTTTCAT 2461 CCAGAAAACC AAAAGGAATC CTCTGGTTTG CTTAATGGGA GTCATCAAGT GTAACAGCAA 2521 CTCCATGTGG ATTGTGTGCC AATTTCCAGT GGAGATGCTG TTACTTTTGA TGGTTACCAA 2581 CTCACTACAA TAGAAAGATC TAAGACAGAA AATCATAGTC ACTTGATTCT AACTTGCTTG 2641 CAAAGAAAGA AGATAACCTT TTGTGCTATC TTTAAAAACT GACAAATAAT TAAGAATTAA 2701 TATCTATCTT CTTAAATCAA GTGTCATGTC ggatccCTGC GATATCCAGC gcggccgcGa 2761 aggatctgcg atcgctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc 2821 gagaagttgg ggggaggggt cggcaattga acgggtgcct agagaaggtg gcgcggggta 2881 aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg 2941 tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca 3001 cagctgaagc ttcgaggggc tcgcatctct ccttcacgcg cccgccgccc tacctgaggc 3061 cgccatccac gccggttgag tcgcgttctg ccgcctcccg cctgtggtgc ctcctgaact 3121 gcgtccgccg tctaggtaag tttaaagctc aggtcgagac cgggcctttg tccggcgctc 3181 ccttggagcc tacctagact cagccggctc tccacgcttt gcctgaccct gcttgctcaa 3241 ctctacgtct ttgtttcgtt ttctgttctg cgccgttaca gatccaagct gtgaccggcg 3301 cctacgctag acgccaccat ggagagcgac gagagcggcc tgcccgccat ggagatcgag 3361 tgccgcatca ccggcaccct gaacggcgtg gagttcgagc tggtgggcgg cggagagggc 3421 acccccaagc agggccgcat gaccaacaag atgaagagca ccaaaggcgc cctgaccttc 3481 agcccctacc tgctgagcca cgtgatgggc tacggcttct accacttcgg cacctacccc 3541 agcggctacg agaacccctt cctgcacgcc atcaacaacg gcggctacac caacacccgc 3601 atcgagaagt acgaggacgg cggcgtgctg cacgtgagct tcagctaccg ctacgaggcc 3661 ggccgcgtga tcggcgactt caaggtggtg ggcaccggct tccccgagga cagcgtgatc 3721 ttcaccgaca agatcatccg cagcaacgcc accgtggagc acctgcaccc catgggcgat 3781 aacgtgctgg tgggcagctt cgcccgcacc ttcagcctgc gcgacggcgg ctactacagc 3841 ttcgtggtgg acagccacat gcacttcaag agcgccatcc accccagcat cctgcagaac 3901 gggggcccca tgttcgcctt ccgccgcgtg gaggagctgc acagcaacac cgagctgggc 3961 atcgtggagt accagcacgc cttcaagacc cccatcgcct tcgccagatc ccgcgctcag 4021 tcgtccaatt ctgccgtgga cggcaccgcc ggacccggct ccaccggatc tcgcgagggc 4081 agaggaagtc ttctaacatg cggtgacgtg gaggagaatc ccggccctat gaccgagtac 4141 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc 4201 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag 4261 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg 4321 tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg 4381 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc 4441 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc 4501 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg 4561 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg 4621 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg 4681 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgaaa tcaacctctg 4741 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta 4801 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt 4861 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc 4921 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt 4981 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg 5041 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac 5101 aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc 5161 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac 5221 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtctccg ccttcgccct 5281 cagacgagtc ggatctccct ttggccgcct ccccgcctgg tacctttaag accaatgact 5341 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta 5401 attcactccc aacgaaaata agatctgctt tttgcttgta ctgggtctct ctggttagac 5461 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa 5521 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag 5581 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat 5641 cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg 5701 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa 5761 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat 5821 gtctggctct agctatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg 5881 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc 5941 agaagtagtg aggaggcttt tttggaggcc tagacttttg cagagacggc ccaaattcgt 6001 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca 6061 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat 6121 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt 6181 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct 6241 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa 6301 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa 6361 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc 6421 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga 6481 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc 6541 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt 6601 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct 6661 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg 6721 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta 6781 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct 6841 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa 6901 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt 6961 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta 7021 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat 7081 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa 7141 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct 7201 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta 7261 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct 7321 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg 7381 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa 7441 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt 7501 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta 7561 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca 7621 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta 7681 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct 7741 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg 7801 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac 7861 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact 7921 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa 7981 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt 8041 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat 8101 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg 8161 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc 8221 cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg 8281 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt 8341 cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac 8401 tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca 8461 tcaggcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct 8521 cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa 8581 cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgccaag ctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.