pLV3-CMV-Fluc-D2HGDH-EF1a-CopG

pLV3-CMV-Fluc-D2HGDH-EF1a-CopGFP-Puro plasmid

PVT107022

pLV3-CMV-Fluc-D2HGDH-EF1a-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-Fluc-D2HGDH(human)-EF1a-CopGFP-Puro
Alias: NM_152783.5;D2HGD
Gene lenght: 1566
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green,Fluc
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: Luc-F(partial HighGC）
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-Fluc-D2HGDH-EF1a-CopGFP-Puro plasmid Description

pLV3-CMV-Fluc-D2HGDH-EF1a-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and is also applicable for lentivirus packaging.

pLV3-CMV-Fluc-D2HGDH-EF1a-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt 1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc 1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga 1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac 1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga 2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg 2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg 2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac 2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagagc 2281 tagcgaattc gccaccatgg aagacgccaa aaacataaag aaaggcccgg cgccattcta 2341 tcctcttgag gatggaaccg ctggagagca actgcataag gctatgaaga gatacgccct 2401 ggttcctgga acaattgctt ttacagatgc acatatcgag gtgaacatca cgtacgcgga 2461 atacttcgaa atgtccgttc ggttggcaga agctatgaaa cgatatgggc tgaatacaaa 2521 tcacagaatc gtcgtatgca gtgaaaactc tcttcaattc tttatgccgg tgttgggcgc 2581 gttatttatc ggagttgcag ttgcgcccgc gaacgacatt tataatgaac gtgaattgct 2641 caacagtatg aacatttcgc agcctaccgt agtgtttgtt tccaaaaagg ggttgcaaaa 2701 aattttgaac gtgcaaaaaa aattaccaat aatccagaaa attattatca tggattctaa 2761 aacggattac cagggatttc agtcgatgta cacgttcgtc acatctcatc tacctcccgg 2821 ttttaatgaa tacgattttg taccagagtc ctttgatcgt gacaaaacaa ttgcactgat 2881 aatgaactcc tctggatcta ctgggttacc taagggtgtg gcccttccgc atagaactgc 2941 ctgcgtcaga ttctcgcatg ccagagatcc tatttttggc aatcaaatca ttccggatac 3001 tgcgatttta agtgttgttc cattccatca cggttttgga atgtttacta cactcggata 3061 tttgatatgt ggatttcgag tcgtcttaat gtatagattt gaagaagagc tgtttttacg 3121 atcccttcag gattacaaaa ttcaaagtgc gttgctagta ccaaccctat tttcattctt 3181 cgccaaaagc actctgattg acaaatacga tttatctaat ttacacgaaa ttgcttctgg 3241 gggcgcacct ctttcgaaag aagtcgggga agcggttgca aaacgcttcc atcttccagg 3301 gatacgacaa ggatatgggc tcactgagac tacatcagct attctgatta cacccgaggg 3361 ggatgataaa ccgggcgcgg tcggtaaagt tgttccattt tttgaagcga aggttgtgga 3421 tctggatacc gggaaaacgc tgggcgttaa tcagagaggc gaattatgtg tcagaggacc 3481 tatgattatg tccggttatg taaacaatcc ggaagcgacc aacgccttga ttgacaagga 3541 tggatggcta cattctggag acatagctta ctgggacgaa gacgaacact tcttcatagt 3601 tgaccgcttg aagtctttaa ttaaatacaa aggataccag gtggcccccg ctgaattgga 3661 gtcgatattg ttacaacacc ccaacatctt cgacgcgggc gtggcaggtc ttcccgacga 3721 tgacgccggt gaacttcccg ccgccgttgt tgttttggag cacggaaaga cgatgacgga 3781 aaaagagatc gtggattacg tcgccagtca agtaacaacc gcgaaaaagt tgcgcggagg 3841 agttgtgttt gtggacgaag taccgaaagg tcttaccgga aaactcgacg caagaaaaat 3901 cagagagatc ctcataaagg ccaagaaggg cggaaagtcc aaattgggtg gaggcggatc 3961 aggaggcggt ggatctggat ccatgctgcc ccgtcggcct ctggcgtggc ccgcgtggct 4021 gttgcggggt gctccgggag ccgcgggttc ttggggtcgg ccggttggcc ccctggcccg 4081 cagaggctgc tgctccgccc cggggacccc cgaggtgccg ctgacccggg agcgctaccc 4141 cgtgcggcgc ttgccgttct ccacggtgtc taagcaggac ctggccgcct ttgagcgcat 4201 cgtgcccggc ggggtcgtca cggacccgga agcgctgcag gctcccaacg tggactggtt 4261 gcggacgctg cgaggctgta gcaaggtgct gctgaggcca cggacgtcgg aggaggtgtc 4321 ccacatcctc aggcactgcc acgagaggaa cctggccgtg aacccacagg ggggcaacac 4381 aggcatggtg ggtggcagcg tccccgtctt tgacgagatc atcctctcca ctgcccgcat 4441 gaaccgggtc ctcagcttcc acagcgtgtc tggaattctg gtttgccagg cgggctgcgt 4501 cctggaggag ctgagccggt atgtggagga acgggacttc atcatgccgc tggacttagg 4561 agccaagggc agctgccaca tcgggggaaa cgtggcaacc aacgctggag gcctgcggtt 4621 tcttcgatat ggctcactgc atgggactgt cctgggcctg gaagtggtgc tggccgacgg 4681 cactgtcctg gactgcctga cctccctgag gaaggacaac acgggctatg acctgaagca 4741 gctgttcatc gggtcggagg gcactttggg gatcatcacc acggtgtcca tcttgtgtcc 4801 acccaagccc agggctgtga acgtggcttt cctcggctgc ccaggctttg ctgaggttct 4861 gcagaccttc agcacctgca aggggatgct gggtgagatc ctgtctgcat tcgagttcat 4921 ggatgctgtg tgcatgcagc tggtcgggcg ccatctccac ctggccagcc cggtgcaaga 4981 gagtccgttt tacgtcctca tcgagacttc aggctccaac gcaggccatg acgctgagaa 5041 gctgggccac ttcctggagc acgcgctggg ctccggcctg gtgaccgatg ggaccatggc 5101 caccgaccag aggaaagtca agatgctgtg ggccctgagg gaaaggatca cagaggcgct 5161 gagccgggat ggctacgtgt acaagtacga cctctccctc cctgtggagc ggctctacga 5221 catcgtgact gacctgcgcg cccgcctcgg cccgcacgcc aagcacgtgg tgggctatgg 5281 ccaccttgga gatggtaacc tgcacctcaa tgtgacggcg gaggccttca gcccctcgct 5341 cctggctgcc ctggagcccc acgtgtacga gtggacggcc gggcagcagg gcagcgtcag 5401 cgcggagcac ggagtgggct tcaggaagag ggacgtcctg ggctacagca agccaccggg 5461 ggccctgcag ctcatgcagc agctcaaggc cctgctggac cccaagggca tcctcaaccc 5521 ctacaagacg ctgcccagcc aggcctgagc ggccgcaagg atctgcgatc gctccggtgc 5581 ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg 5641 caattgaacg ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta 5701 ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt 5761 gaacgttctt tttcgcaacg ggtttgccgc cagaacacag ctgaagcttc gaggggctcg 5821 catctctcct tcacgcgccc gccgccctac ctgaggccgc catccacgcc ggttgagtcg 5881 cgttctgccg cctcccgcct gtggtgcctc ctgaactgcg tccgccgtct aggtaagttt 5941 aaagctcagg tcgagaccgg gcctttgtcc ggcgctccct tggagcctac ctagactcag 6001 ccggctctcc acgctttgcc tgaccctgct tgctcaactc tacgtctttg tttcgttttc 6061 tgttctgcgc cgttacagat ccaagctgtg accggcgcct acgctagacg ccaccatgga 6121 gagcgacgag agcggcctgc ccgccatgga gatcgagtgc cgcatcaccg gcaccctgaa 6181 cggcgtggag ttcgagctgg tgggcggcgg agagggcacc cccaagcagg gccgcatgac 6241 caacaagatg aagagcacca aaggcgccct gaccttcagc ccctacctgc tgagccacgt 6301 gatgggctac ggcttctacc acttcggcac ctaccccagc ggctacgaga accccttcct 6361 gcacgccatc aacaacggcg gctacaccaa cacccgcatc gagaagtacg aggacggcgg 6421 cgtgctgcac gtgagcttca gctaccgcta cgaggccggc cgcgtgatcg gcgacttcaa 6481 ggtggtgggc accggcttcc ccgaggacag cgtgatcttc accgacaaga tcatccgcag 6541 caacgccacc gtggagcacc tgcaccccat gggcgataac gtgctggtgg gcagcttcgc 6601 ccgcaccttc agcctgcgcg acggcggcta ctacagcttc gtggtggaca gccacatgca 6661 cttcaagagc gccatccacc ccagcatcct gcagaacggg ggccccatgt tcgccttccg 6721 ccgcgtggag gagctgcaca gcaacaccga gctgggcatc gtggagtacc agcacgcctt 6781 caagaccccc atcgccttcg ccagatcccg cgctcagtcg tccaattctg ccgtggacgg 6841 caccgccgga cccggctcca ccggatctcg cgagggcaga ggaagtcttc taacatgcgg 6901 tgacgtggag gagaatcccg gccctatgac cgagtacaag cccacggtgc gcctcgccac 6961 ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc 7021 cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact 7081 cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc 7141 ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg 7201 cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct 7261 cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc 7321 cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga 7381 gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga 7441 gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg 7501 catgacccgc aagcccggtg cctgaaatca acctctggat tacaaaattt gtgaaagatt 7561 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc 7621 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg 7681 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac 7741 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc 7801 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc 7861 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa 7921 gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc 7981 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc 8041 ggctctgcgg cctcttccgc gtctccgcct tcgccctcag acgagtcgga tctccctttg 8101 gccgcctccc cgcctggtac ctttaagacc aatgacttac aaggcagctg tagatcttag 8161 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaaaataaga 8221 tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc 8281 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt 8341 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc 8401 agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt atttataact 8461 tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta 8521 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag 8581 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc 8641 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat 8701 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt 8761 ggaggcctag acttttgcag agacggccca aattcgtaat catggtcata gctgtttcct 8821 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt 8881 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc 8941 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg 9001 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg 9061 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca 9121 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac 9181 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac 9241 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg 9301 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac 9361 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat 9421 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag 9481 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac 9541 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt 9601 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt 9661 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc 9721 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga 9781 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac 9841 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc 9901 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct 9961 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca 10021 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct 10081 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca 10141 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc 10201 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg 10261 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct 10321 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa 10381 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta 10441 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc 10501 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg 10561 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa 10621 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg 10681 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc 10741 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg 10801 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat 10861 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata 10921 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc 10981 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt 11041 gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa 11101 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg 11161 ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt 11221 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc gccattcagg 11281 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg 11341 aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga 11401 cgttgtaaaa cgacggccag tgccaagctg

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.