pCMV-ZNF532-3×FLAG-Neo plasmid

pCMV-ZNF532-3×FLAG-Neo plasmid

PVT106479

pCMV-ZNF532-3×FLAG-Neo plasmid Information

Plasmid name: pCMV-ZNF532(human-toxic-opt)-3×FLAG-Neo
Alias: NM_018181.6
Gene lenght: 3903
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-ZNF532-3×FLAG-Neo plasmid Description

pCMV-ZNF532-3×FLAG-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-ZNF532-3×FLAG-Neo plasmid Sequence

1 GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA 61 TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG 121 TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC 181 CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA 241 CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT 301 ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC 361 CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT 421 ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA 481 CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT 541 CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG 601 CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTCTGGC TAACTAGAGA ACCCACTGCT 661 TACTGGCTTA TCGAAATTAA TACGACTCAC TATAGGGAGA CCCAAGCTGG CTAGCGTTTA 721 AACTTAAGCT TGGTACCGAG CTCGAATTCG CCACCatgac catgggggat atgaagaccc 781 cagactttga tgacctcctg gcagcatttg acatcccaga tatggtcgat cctaaagcag 841 ctattgagtc tggacacgat gaccatgaaa gccacatgaa gcagaatgct cacggagagg 901 atgactccca cgcaccatca tcttctgatg tgggtgtcag cgttatcgtc aagaatgttc 961 ggaacattga ctcttccgag ggcggggaga aagacggcca caaccccact ggcaatggct 1021 tacataatgg gtttctcaca gcatcctccc ttgacagtta cagtaaagat ggagcaaagt 1081 ccttgaaagg agatgtgcct gcctctgagg tgacactgaa agactcgaca ttcagccagt 1141 ttagcccgat ctccagtgct gaagagtttg atgacgacga gaagattgag gtggatgacc 1201 cccctgacaa ggaggacatg cgatcaagct tcaggtcgaa tgtgttgacg gggtcggctc 1261 cccagcagga ctacgataag ctgaaggcac tcggagggga aaactccagc aaaactggac 1321 tctctacgtc aggcaatgtg gagaaaaaca aagctgttaa gagagaaaca gaagccagtt 1381 ctataaacct gagtgtttat gaacctttta aagtcagaaa agcagaggat aaattgaagg 1441 aaagctctga caaggtgctg gaaaacagag tcctagatgg gaagctgagc tccgagaaga 1501 atgacaccag cctccccagc gttgcgccat caaagacaaa gtcgtcctcc aagctctcgt 1561 cctgcatcgc tgccatcgcg gctctcagcg ctaaaaaggc ggcttcagac tcctgcaaag 1621 aaccagtggc caattcgagg gaatcctccc cgttaccaaa agaagtaaat gacagtccga 1681 gagccgctga caagtctcct gaatcccaga atctcatcga cgggaccaaa aaaccatccc 1741 tgaagcaacc ggatagtccc agaagcatct caagtgagaa cagcagcaaa ggatccccgt 1801 cctctcccgc agggtccaca ccagcaatcc ccaaagtccg cataaaaacc attaagacat 1861 cttctgggga aatcaagaga acagtgacca gggtattgcc agaagtggat cttgactctg 1921 gaaagaaacc ttccgagcag acagcgtccg tgatggcctc tgtgacatcc cttctgtcgt 1981 ctccagcatc agccgccgtc ctttcctctc cccccagggc gcctctccag tctgcggtcg 2041 tgaccaatgc agtttcccct gcagagctca cccccaaaca ggtcacaatc aagcctgtgg 2101 ctactgcttt cctcccagtg tctgctgtga agacggcagg atcccaagtc attaatttga 2161 agctcgctaa caacaccacg gtgaaagcca cggtcatatc tgctgcctct gtccagagtg 2221 ccagcagcgc catcattaaa gctgccaacg ccatccagca gcaaactgtc gtggtgccgg 2281 catccagcct ggccaatgcc aaactcgtgc caaagactgt gcaccttgcc aaccttaacc 2341 ttttgcctca gggtgcccag gccacctctg aactccgcca agtgctaacc aaacctcagc 2401 aacaaataaa gcaggcaata atcaatgcag cagcctcgca accccccaaa aaggtgtctc 2461 gagtccaggt ggtgtcgtcc ttgcagagtt ctgtggtgga agctttcaac aaggtgctga 2521 gcagtgtcaa tccagtccct gtttacatcc caaacctcag tcctcccgcc aatgcaggga 2581 tcacgttacc gacgcgtggg tacaagtgct tggagtgtgg ggactccttt gcacttgaaa 2641 agagtctgac ccagcactac gacagacgga gcgtgcgcat cgaagtaacg tgcaaccatt 2701 gtacaaagaa cctcgttttt tacaacaaat gcagcctcct ttcccatgcc cgtgggcata 2761 aggagaaagg ggtggtaatg caatgctccc acttaatttt aaagccagtc ccagcagatc 2821 aaatgatagt ttctccgtca agcaatactt ccacttcaac ttccactctt cagagccctg 2881 tgggagctgg cacacacact gtcacaaaaa ttcagtctgg cataactggg acagtcatat 2941 cggctccttc aagcactccc atcaccccag ccatgcccct agatgaagac ccctccaaac 3001 tgtgtagaca tagtctaaaa tgtttggagt gtaatgaagt cttccaggac gagacatcac 3061 tggctacaca tttccagcag gctgcagata cgagtggaca aaagacttgc actatctgcc 3121 agatgctgct tcctaaccag tgcagttatg catcacacca gagaatccat cagcacaaat 3181 ctccctacac ctgccctgag tgtggggcca tctgcaggtc ggtgcacttc cagacccacg 3241 tcaccaagaa ctgtctgcac tacacgagga gagttggttt tcgatgtgtg cattgcaatg 3301 ttgtgtactc tgatgtggct gctctgaagt ctcacattca aggttctcac tgtgaagtct 3361 tctacaagtg tcctatttgt ccaatggcgt ttaagtctgc cccaagcaca CACAGCCACG 3421 CATACACCCA GCACCCCGGC ATCAAGATCG GGGAACCCAA GATCATCTAC AAGTGCTCCA 3481 TGTGCGACAC CGTGTTCACA CTTCAGACCC TGCTGTACAG GCATTTCGAT CAGCACATCG 3541 AGAATCAGAA AGTGTCCGTG TTTAAGTGCC CTGATTGCTC CCTGCTGTAC GCTCAGAAGC 3601 AGCTGATGAT GGACCACATT AAGAGCATGC ACGGCACACT GAAGAGTATT GAGGGACCTC 3661 CTAACCTGGG GATCAATCTG CCCCTGTCAA TCAAGCCCGC CACTCAGAAT AGCGCCAACC 3721 AGAATAAAGA GGACACCAAG AGCATGAACG GCAAGGAGAA GCTGGAGAAG AAGAGCCCCA 3781 GCCCAGTGAA AAAGTCCATG GAGACCAAAA AAGTGGCCTC CCCCGGGTGG ACCTGTTGGG 3841 AGTGCGACTG CCTGTTCATG CAGCGGGACG TGTACATCTC CCATGTGCGG AAGGAGCACG 3901 GCAAACAGAT GAAAAAGCAC CCATGTAGGC AGTGCGACAA GAGCTTTAGC TCCTCCCACA 3961 GCCTGTGTAG ACACAATAGG ATCAAGCACA AAGGCATCCG GAAGGTGTAT GCCTGTAGTC 4021 ACTGCCCAGA CTCCCGCAGG ACCTTCACTA AGCGCCTGAT GCTGGAGAAG CACGTGCAGC 4081 TGATGCACGG CATTAAGGAC CCAGATCTGA AGGAGATGAC TGACGCCACC AACGAGGAGG 4141 AGACCGAAAT CAAGGAAGAC ACAAAAGTGC CCAGCCCCAA GAGGAAGCTG GAGGAGCCCG 4201 TGCTCGAGTT CAGGCCTCCT CGGGGCGCCA TCACCCAGCC CCTGAAGAAG CTGAAGATCA 4261 ATGTGTTTAA GGTGCACAAG TGCGCCGTGT GCGGCTTCAC AACAGAGAAC CTGCTGCAGT 4321 TTCACGAGCA CATCCCCCAG CACAAGTCTG ATGGGTCCTC CTATCAGTGC CGCGAGTGTG 4381 GCCTGTGCTA CACCTCCCAC GTGTCCCTGt ccaggcacct cttcatcgta cacaagttaa 4441 aggaacctca gccagtgtcc aagcaaaatg gggctgggga agataaccaa caggagaaca 4501 aacccagcca cgaggatgaa tcccctgatg gcgccgtgtc agacagaaag tgcaaagtgt 4561 gcgcaaaaac ttttgaaact gaagctgcct taaatactca catgcggaca cacggcatgg 4621 ccttcatcaa atccaaaagg atgagctcag ccgagaaaGG ATCCGACTAC AAAGACCATG 4681 ACGGTGATTA TAAAGATCAT GACATCGACT ACAAGGATGA CGATGACAAG TAGTGAGGGC 4741 CCGATATCTC GACAATCAAC CTCTGGATTA CAAAATTTGT GAAAGATTGA CTGGTATTCT 4801 TAACTATGTT GCTCCTTTTA CGCTATGTGG ATACGCTGCT TTAATGCCTT TGTATCATGC 4861 TATTGCTTCC CGTATGGCTT TCATTTTCTC CTCCTTGTAT AAATCCTGGT TGCTGTCTCT 4921 TTATGAGGAG TTGTGGCCCG TTGTCAGGCA ACGTGGCGTG GTGTGCACTG TGTTTGCTGA 4981 CGCAACCCCC ACTGGTTGGG GCATTGCCAC CACCTGTCAG CTCCTTTCCG GGACTTTCGC 5041 TTTCCCCCTC CCTATTGCCA CGGCGGAACT CATCGCCGCC TGCCTTGCCC GCTGCTGGAC 5101 AGGGGCTCGG CTGTTGGGCA CTGACAATTC CGTGGTGTTG TCGGGGAAGC TGACGTCCTT 5161 TCCATGGCTG CTCGCCTGTG TTGCCACCTG GATTCTGCGC GGGACGTCCT TCTGCTACGT 5221 CCCTTCGGCC CTCAATCCAG CGGACCTTCC TTCCCGCGGC CTGCTGCCGG CTCTGCGGCC 5281 TCTTCCGCGT CTTCGCCTTC GCCCTCAGAC GAGTCGGATC TCCCTTTGGG CCGCCTCCCC 5341 GCCTGGAAAC GGGGGAGGCT AACTGAAACA CGGAAGGAGA CAATACCGGA AGGAACCCGC 5401 GCTATGACGG CAATAAAAAG ACAGAATAAA ACGCACGGGT GTTGGGTCGT TTGTTCATAA 5461 ACGCGGGGTT CGGTCCCAGG GCTGGCACTC TGTCGATACC CCACCGAGAC CCCATTGGGG 5521 CCAATACGCC CGCGTTTCTT CCTTTTCCCC ACCCCACCCC CCAAGTTCGG GTGAAGGCCC 5581 AGGGCTCGCA GCCAACGTCG GGGCGGCAGG CCCTGCCATA GCAGATCTGC GCAGCTGGGG 5641 CTCTAGGGGG TATCCCCACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT 5701 TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT 5761 CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGCATCCC 5821 TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA 5881 TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC 5941 CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT 6001 CTATTCTTTT GATTTATAAG GGATTTTGGG GATTTCGGCC TATTGGTTAA AAAATGAGCT 6061 GATTTAACAA AAATTTAACG CGAATTAATT CTGTGGAATG TGTGTCAGTT AGGGTGTGGA 6121 AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA 6181 ACCAGGTGTG GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA GTATGCAAAG CATGCATCTC 6241 AATTAGTCAG CAACCATAGT CCCGCCCCTA ACTCCGCCCA TCCCGCCCCT AACTCCGCCC 6301 AGTTCCGCCC ATTCTCCGCC CCATGGCTGA CTAATTTTTT TTATTTATGC AGAGGCCGAG 6361 GCCGCCTCTG CCTCTGAGCT ATTCCAGAAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC 6421 TTTTGCAAAA AGCTCCCGGG AGCTTGTATA TCCATTTTCG GATCTGATCA AGAGACAGGA 6481 TGAGGATCGT TTCGCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC GGCCGCTTGG 6541 GTGGAGAGGC TATTCGGCTA TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC 6601 GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT 6661 GCCCTGAATG AACTGCAGGA CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT 6721 CCTTGCGCAG CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC 6781 GAAGTGCCGG GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA AGTATCCATC 6841 ATGGCTGATG CAATGCGGCG GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC 6901 CAAGCGAAAC ATCGCATCGA GCGAGCACGT ACTCGGATGG AAGCCGGTCT TGTCGATCAG 6961 GATGATCTGG ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG AACTGTTCGC CAGGCTCAAG 7021 GCGCGCATGC CCGACGGCGA GGATCTCGTC GTGACCCATG GCGATGCCTG CTTGCCGAAT 7081 ATCATGGTGG AAAATGGCCG CTTTTCTGGA TTCATCGACT GTGGCCGGCT GGGTGTGGCG 7141 GACCGCTATC AGGACATAGC GTTGGCTACC CGTGATATTG CTGAAGAGCT TGGCGGCGAA 7201 TGGGCTGACC GCTTCCTCGT GCTTTACGGT ATCGCCGCTC CCGATTCGCA GCGCATCGCC 7261 TTCTATCGCC TTCTTGACGA GTTCTTCTGA GCGGGACTCT GGGGTTCGCG AAATGACCGA 7321 CCAAGCGACG CCCAACCTGC CATCACGAGA TTTCGATTCC ACCGCCGCCT TCTATGAAAG 7381 GTTGGGCTTC GGAATCGTTT TCCGGGACGC CGGCTGGATG ATCCTCCAGC GCGGGGATCT 7441 CATGCTGGAG TTCTTCGCCC ACCCCAACTT GTTTATTGCA GCTTATAATG GTTACAAATA 7501 AAGCAATAGC ATCACAAATT TCACAAATAA AGCATTTTTT TCACTGCATT CTAGTTGTGG 7561 TTTGTCCAAA CTCATCAATG TATCTTATCA TGTCTGTATA CCGTCGACCT CTAGCTAGAG 7621 CTTGGCGTAA TCATGGTCAT AGCTGTTTCC TGTGTGAAAT TGTTATCCGC TCACAATTCC 7681 ACACAACATA CGAGCCGGAA GCATAAAGTG TAAAGCCTGG GGTGCCTAAT GAGTGAGCTA 7741 ACTCACATTA ATTGCGTTGC GCTCACTGCC CGCTTTCCAG TCGGGAAACC TGTCGTGCCA 7801 GCTGCATTAA TGAATCGGCC AACGCGCGGG GAGAGGCGGT TTGCGTATTG GGCGCTCTTC 7861 CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC 7921 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 7981 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 8041 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 8101 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 8161 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 8221 GGCGCTTTCT CAATGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 8281 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 8341 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 8401 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 8461 CTACGGCTAC ACTAGAAGGA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 8521 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 8581 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 8641 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 8701 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 8761 AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC 8821 ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA 8881 GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA 8941 CCCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG 9001 CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC 9061 TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT 9121 CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG 9181 GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT 9241 CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA 9301 TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA 9361 GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA 9421 TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG 9481 GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC 9541 ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG 9601 AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT 9661 CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT 9721 ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT 9781 GCCACCTGAC GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTCGAC TCTCAGTACA 9841 ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC 9901 GCTGAGTAGT GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC 9961 ATGAAGAATC TGCTTAGG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.