pLV3-CMV-3×HA-PFKP-CopGFP-Neo

pLV3-CMV-3×HA-PFKP-CopGFP-Neo plasmid

PVT105708

pLV3-CMV-3×HA-PFKP-CopGFP-Neo plasmid Information

Plasmid name: pLV3-CMV-3×HA-PFKP(human)-CopGFP-Neo
Alias: NM_002627.5;ATP-PFK; PFK-C; PFK-P; PFKF;
Gene lenght: 2355
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-3×HA-PFKP-CopGFP-Neo plasmid Description

pLV3-CMV-3×HA-PFKP-CopGFP-Neo It is a plasmid suitable for protein expression in mammalian cells and is also applicable for lentivirus packaging.

pLV3-CMV-3×HA-PFKP-CopGFP-Neo plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc gaattcgcca ccatgTACCC ATACGACGTA CCTGATTATG CAACTTATCC 2341 TTATGATGTA CCTGATTATG CTAGtTATCC TTATGATGTC CCAGACTACG CActcgagat 2401 ggacgcggac gactcccggg cccccaaggg ctccttgcgg aagttcctgg agcacctctc 2461 cggggccggc aaggccatcg gcgtgctgac cagcggcggg gatgctcaag gtatgaacgc 2521 tgccgtccgt gccgtggtgc gcatgggtat ctacgtgggg gccaaggtgt acttcatcta 2581 cgagggctac cagggcatgg tggacggagg ctcaaacatc gcagaggccg actgggagag 2641 tgtctccagc atcctgcaag tgggcgggac gatcattggc agtgcgcggt gccaggcctt 2701 ccgcacgcgg gaaggccgcc tgaaggctgc ttgcaacctg ctgcagcgcg gcatcaccaa 2761 cctgtgtgtg atcggcgggg acgggagcct caccggggcc aacctcttcc ggaaggagtg 2821 gagtgggctg ctggaggagc tggccaggaa cggccagatc gataaggagg ccgtgcagaa 2881 gtacgcctac ctcaacgtgg tgggcatggt gggctccatc gacaatgatt tctgcggcac 2941 cgacatgacc atcggcacgg actccgccct gcacaggatc atcgaggtcg tcgacgccat 3001 catgaccacg gcccagagcc accagaggac cttcgttctg gaggtgatgg gacgacactg 3061 tgggtacctg gccctggtga gtgccttggc ctgcggtgcg gactgggtgt tccttccaga 3121 atctccacca gaggaaggct gggaggagca gatgtgtgtc aaactctcgg agaaccgtgc 3181 ccggaaaaaa aggctgaata ttattattgt ggctgaagga gcaattgata cccaaaataa 3241 acccatcacc tctgagaaaa tcaaagagct tgtcgtcacg cagctgggct atgacacacg 3301 tgtgaccatc ctcgggcacg tgcagagagg agggacccct tcggcattcg acaggatctt 3361 ggccagccgc atgggagtgg aggcagtcat cgccttgcta gaggccaccc cggacacccc 3421 agcttgcgtc gtgtcactga acgggaacca cgccgtgcgc ctgccgctga tggagtgcgt 3481 gcagatgact caggatgtgc agaaggcgat ggacgagagg agatttcaag atgcggttcg 3541 actccgaggg aggagctttg cgggcaacct gaacacctac aagcgacttg ccatcaagct 3601 gccggatgat cagatcccaa agaccaattg caacgtagct gtcatcaacg tgggggcacc 3661 cgcggctggg atgaacgcag ccgtacgctc agctgtgcgc gtgggcattg ccgacggcca 3721 caggatgctc gccatctatg atggctttga cggcttcgcc aagggccaga tcaaagaaat 3781 cggctggaca gatgtcgggg gctggaccgg ccaaggaggc tccattcttg ggacaaaacg 3841 cgttctcccg gggaagtact tggaagagat cgccacacag atgcgcacgc acagcatcaa 3901 cgcgctgctg atcatcggtg gattcgaggc ctacctggga ctcctggagc tgtcagccgc 3961 ccgggagaag cacgaggagt tctgtgtccc catggtcatg gttcccgcta ctgtgtccaa 4021 caatgtgccg ggttccgatt tcagcatcgg ggcagacacc gccctgaaca ctatcaccga 4081 cacctgcgac cgcatcaagc agtccgccag cggaaccaag cggcgcgtgt tcatcatcga 4141 gaccatgggc ggctactgtg gctacctggc caacatgggg gggctcgcgg ccggagctga 4201 tgccgcatac attttcgaag agcccttcga catcagggat ctgcagtcca acgtggagca 4261 cctgacggag aaaatgaaga ccaccatcca gagaggcctt gtgctcagaa atgagagctg 4321 cagtgaaaac tacaccaccg acttcattta ccagctgtat tcagaagagg gcaaaggcgt 4381 gtttgactgc aggaagaacg tgctgggtca catgcagcag ggtggggcac cctctccatt 4441 tgatagaaac tttggaacca aaatctctgc cagagctatg gagtggatca ctgcaaaact 4501 caaggaggcc cggggcagag gaaaaaaatt taccaccgat gattccattt gtgtgctggg 4561 aataagcaaa agaaacgtta tttttcaacc tgtggcagag ctgaagaagc aaacggattt 4621 tgagcacagg attcccaaag aacagtggtg gctcaagcta cggcccctca tgaaaatcct 4681 ggccaagtac aaggccagct atgacgtgtc ggactcaggc cagctggaac atgtgcagcc 4741 ctggagtgtc tgaggatccg cggccgcGaa ggatctgcga tcgctccggt gcccgtcagt 4801 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa 4861 cgggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc 4921 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc 4981 tttttcgcaa cgggtttgcc gccagaacac agctgaagct tcgaggggct cgcatctctc 5041 cttcacgcgc ccgccgccct acctgaggcc gccatccacg ccggttgagt cgcgttctgc 5101 cgcctcccgc ctgtggtgcc tcctgaactg cgtccgccgt ctaggtaagt ttaaagctca 5161 ggtcgagacc gggcctttgt ccggcgctcc cttggagcct acctagactc agccggctct 5221 ccacgctttg cctgaccctg cttgctcaac tctacgtctt tgtttcgttt tctgttctgc 5281 gccgttacag atccaagctg tgaccggcgc ctacgctaga cgccaccatg gagagcgacg 5341 agagcggcct gcccgccatg gagatcgagt gccgcatcac cggcaccctg aacggcgtgg 5401 agttcgagct ggtgggcggc ggagagggca cccccaagca gggccgcatg accaacaaga 5461 tgaagagcac caaaggcgcc ctgaccttca gcccctacct gctgagccac gtgatgggct 5521 acggcttcta ccacttcggc acctacccca gcggctacga gaaccccttc ctgcacgcca 5581 tcaacaacgg cggctacacc aacacccgca tcgagaagta cgaggacggc ggcgtgctgc 5641 acgtgagctt cagctaccgc tacgaggccg gccgcgtgat cggcgacttc aaggtggtgg 5701 gcaccggctt ccccgaggac agcgtgatct tcaccgacaa gatcatccgc agcaacgcca 5761 ccgtggagca cctgcacccc atgggcgata acgtgctggt gggcagcttc gcccgcacct 5821 tcagcctgcg cgacggcggc tactacagct tcgtggtgga cagccacatg cacttcaaga 5881 gcgccatcca ccccagcatc ctgcagaacg ggggccccat gttcgccttc cgccgcgtgg 5941 aggagctgca cagcaacacc gagctgggca tcgtggagta ccagcacgcc ttcaagaccc 6001 ccatcgcctt cgccagatcc cgcgctcagt cgtccaattc tgccgtggac ggcaccgccg 6061 gacccggctc caccggatct cgcgagggca gaggaagtct tctaacatgc ggtgacgtgg 6121 aggagaatcc cggccctGCC ACCatggctg aacaagatgg attgcacgca ggttctccgg 6181 ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg 6241 atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc 6301 tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga 6361 cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc 6421 tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag 6481 tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat 6541 tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg 6601 tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca 6661 ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct 6721 tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg 6781 gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg 6841 gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc 6901 gcatcgcctt ctatcgcctt cttgacgagt tcttctgaGT CGACAATCAA CCTCTGGATT 6961 ACAAAATTTG TGAAAGATTG ACTGGTATTC TTAACTATGT TGCTCCTTTT ACGCTATGTG 7021 GATACGCTGC TTTAATGCCT TTGTATCATG CTATTGCTTC CCGTATGGCT TTCATTTTCT 7081 CCTCCTTGTA TAAATCCTGG TTGCTGTCTC TTTATGAGGA GTTGTGGCCC GTTGTCAGGC 7141 AACGTGGCGT GGTGTGCACT GTGTTTGCTG ACGCAACCCC CACTGGTTGG GGCATTGCCA 7201 CCACCTGTCA GCTCCTTTCC GGGACTTTCG CTTTCCCCCT CCCTATTGCC ACGGCGGAAC 7261 TCATCGCCGC CTGCCTTGCC CGCTGCTGGA CAGGGGCTCG GCTGTTGGGC ACTGACAATT 7321 CCGTGGTGTT GTCGGGGAAA TCATCGTCCT TTCCTTGGCT GCTCGCCTGT GTTGCCACCT 7381 GGATTCTGCG CGGGACGTCC TTCTGCTACG TCCCTTCGGC CCTCAATCCA GCGGACCTTC 7441 CTTCCCGCGG CCTGCTGCCG GCTCTGCGGC CTCTTCCGCG TCTTCGCCTT CGCCCTCAGA 7501 CGAGTCGGAT CTCCCTTTGG GCCGCCTCCC CGCggtacct ttaagaccaa tgacttacaa 7561 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca 7621 ctcccaacga aGataagatc tgctttttgc ttgtactggg tctctctggt tagaccagat 7681 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt 7741 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc 7801 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat 7861 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat 7921 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt 7981 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg 8041 gctctagcta tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga 8101 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag 8161 tagtgaggag gcttttttgg aggcctagac ttttgcagag acggcccaaa ttcgtaatca 8221 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga 8281 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt 8341 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga 8401 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc 8461 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg 8521 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc 8581 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc 8641 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga 8701 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc 8761 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat 8821 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg 8881 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc 8941 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga 9001 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact 9061 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt 9121 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag 9181 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg 9241 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa 9301 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata 9361 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg 9421 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata 9481 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg 9541 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct 9601 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt 9661 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc 9721 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga 9781 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt 9841 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc 9901 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa 9961 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca 10021 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca 10081 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct 10141 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc 10201 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa 10261 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt 10321 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc 10381 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt 10441 cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg 10501 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg 10561 ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga 10621 gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg 10681 cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg 10741 ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca 10801 gggttttccc agtcacgacg ttgtaaaacg acggccagtg ccaagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.