pLV2-EF1a-Mito-EGFP-IRES-Hyg p

pLV2-EF1a-Mito-EGFP-IRES-Hyg plasmid

PVT105110

pLV2-EF1a-Mito-EGFP-IRES-Hyg plasmid Information

Plasmid name: pLV2-EF1a-Mito-EGFP-IRES-Hyg
Alias:
Gene lenght:
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Empty Vector
Prokaryotic resistance: Amp
Screening marker: Hyg
Fluorescent labeling: Green
Promoter: EF-1a
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: EF-1α-F1
Reverse primer:
Copy number: High
Induction:

pLV2-EF1a-Mito-EGFP-IRES-Hyg plasmid Description

pLV2-EF1a-Mito-EGFP-IRES-Hyg It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV2-EF1a-Mito-EGFP-IRES-Hyg plasmid Sequence

1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgaggt gcccgtcagt 2221 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa 2281 ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc 2341 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc 2401 tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc 2461 ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc tggctgcagt 2521 acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg 2581 cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc 2641 gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca 2701 tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg 2761 cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc 2821 gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg 2881 gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta 2941 tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat 3001 ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc tcgggagagc 3061 gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat 3121 gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg agcttttgga 3181 gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt 3241 gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc 3301 tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc 3361 ttccatttca ggtgtcgtga ggatccgcca ccATGTCCGT CCTGACGCCG CTGCTGCTGC 3421 GGGGCTTGAC AGGCTCGGCC CGGCGGCTCC CAGTGCCGCG CGCCAAGATC CATTCGTTGG 3481 GGGATCCACC GGTCGCCACC TGCGGCCGCG GAGGATCCGG AGGAGAATTC ACCATGGTGA 3541 GCAAGGGCGA GGAGCTGTTC ACCGGGGTGG TGCCCATCCT GGTCGAGCTG GACGGCGACG 3601 TAAACGGCCA CAAGTTCAGC GTGTCCGGCG AGGGCGAGGG CGATGCCACC TACGGCAAGC 3661 TGACCCTGAA GTTCATCTGC ACCACCGGCA AGCTGCCCGT GCCCTGGCCC ACCCTCGTGA 3721 CCACCCTGAC CTACGGCGTG CAGTGCTTCA GCCGCTACCC CGACCACATG AAGCAGCACG 3781 ACTTCTTCAA GTCCGCCATG CCCGAAGGCT ACGTCCAGGA GCGCACCATC TTCTTCAAGG 3841 ACGACGGCAA CTACAAGACC CGCGCCGAGG TGAAGTTCGA GGGCGACACC CTGGTGAACC 3901 GCATCGAGCT GAAGGGCATC GACTTCAAGG AGGACGGCAA CATCCTGGGG CACAAGCTGG 3961 AGTACAACTA CAACAGCCAC AACGTCTATA TCATGGCCGA CAAGCAGAAG AACGGCATCA 4021 AGGTGAACTT CAAGATCCGC CACAACATCG AGGACGGCAG CGTGCAGCTC GCCGACCACT 4081 ACCAGCAGAA CACCCCCATC GGCGACGGCC CCGTGCTGCT GCCCGACAAC CACTACCTGA 4141 GCACCCAGTC CGCCCTGAGC AAAGACCCCA ACGAGAAGCG CGATCACATG GTCCTGCTGG 4201 AGTTCGTGAC CGCCGCCGGG ATCACTCTCG GCATGGACGA GCTGTACAAG taagaattcc 4261 tcgagggcgg ccgctctaga gtcgacgggc cgcggtaaca attgttaact aacttaagct 4321 agcaacggtt tccctctagc gggatcaatt ccgccccccc cccctaacgt tactggccga 4381 agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac catattgccg 4441 tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg 4501 ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt 4561 cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag gcagcggaac 4621 cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga tacacctgca 4681 aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag agtcaaatgg 4741 ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc ccattgtatg 4801 ggatctgatc tggggcctcg gtgcacatgc tttacatgtg tttagtcgag gttaaaaaaa 4861 cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg ataataccat 4921 gaaaaagcct gaactcaccg cgacgtctgt cgagaagttt ctgatcgaaa agttcgacag 4981 cgtctccgac ctgatgcagc tctcggaggg cgaagaatct cgtgctttca gcttcgatgt 5041 aggagggcgt ggatatgtcc tgcgggtaaa tagctgcgcc gatggtttct acaaagatcg 5101 ttatgtttat cggcactttg catcggccgc gctcccgatt ccggaagtgc ttgacattgg 5161 ggaatttagc gagagcctga cctattgcat ctcccgccgt gcacagggtg tcacgttgca 5221 agacctgcct gaaaccgaac tgcccgctgt tctgcagccg gtcgcggagg ccatggatgc 5281 gatcgctgcg gccgatctta gccagacgag cgggttcggc ccattcggac cgcaaggaat 5341 cggtcaatac actacatggc gtgatttcat atgcgcgatt gctgatcccc atgtgtatca 5401 ctggcaaact gtgatggacg acaccgtcag tgcgtccgtc gcgcaggctc tcgatgagct 5461 gatgctttgg gccgaggact gccccgaagt ccggcacctc gtgcacgcgg atttcggctc 5521 caacaatgtc ctgacggaca atggccgcat aacagcggtc attgactgga gcgaggcgat 5581 gttcggggat tcccaatacg aggtcgccaa catcttcttc tggaggccgt ggttggcttg 5641 tatggagcag cagacgcgct acttcgagcg gaggcatccg gagcttgcag gatcgccgcg 5701 gctccgggcg tatatgctcc gcattggtct tgaccaactc tatcagagct tggttgacgg 5761 caatttcgat gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc gatccggagc 5821 cgggactgtc gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga ccgatggctg 5881 tgtagaagta ctcgccgata gtggaaaccg acgccccagc actcgtccga gggcaaagga 5941 atagcccggg cggggcgcgt ctggaacaat caacctctgg attacaaaat ttgtgaaaga 6001 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg 6061 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc 6121 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc 6181 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt 6241 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt 6301 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg 6361 aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg 6421 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg 6481 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt 6541 tgggccgcct ccccgcctgg aattaattct gcagtcgaga cctagaaaaa catggagcaa 6601 tcacaagtag caatacagca gctaccaatg ctgattgtgc ctggctagaa gcacaagagg 6661 aggaggaggt gggttttcca gtcacacctc aggtaccttt aagaccaatg acttacaagg 6721 cagctgtaga tcttagccac tttttaaaag aaaagagggg actggaaggg ctaattcact 6781 cccaacgaag acaagatatc cttgatctgt ggatctacca cacacaaggc tacttccctg 6841 attagcagaa ctacacacca gggccagggg tcagatatcc actgaccttt ggatggtgct 6901 acaagctagt accagttgag ccagataagg tagaagaggc caataaagga gagaacacca 6961 gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa gtgttagagt 7021 ggaggtttga cagccgccta gcatttcatc acgtggcccg agagctgcat ccggagtact 7081 tcaagaactg ctgatatcga gcttgctaca agggactttc cgctggggac tttccaggga 7141 ggcgtggcct gggcgggact ggggagtggc gagccctcag atcctgcata taagcagctg 7201 ctttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc 7261 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg 7321 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg 7381 tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca 7441 aagaaatgaa tatcagagag tgagaggcct tgacattgct agcgtttacc gtcgacctct 7501 agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc 7561 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga 7621 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg 7681 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg 7741 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg 7801 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga 7861 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg 7921 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag 7981 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc 8041 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg 8101 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt 8161 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc 8221 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc 8281 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg 8341 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca 8401 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc 8461 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat 8521 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt 8581 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt 8641 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc 8701 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc 8761 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata 8821 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg 8881 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc 8941 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct 9001 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa 9061 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt 9121 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca 9181 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac 9241 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca 9301 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt 9361 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc 9421 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca 9481 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata 9541 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc 9601 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc 9661 cgaaaagtgc cacctgacgt cgacggatcg ggagatcaac ttgtttattg cagcttataa 9721 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca 9781 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tcaactggat 9841 aactcaagct aaccaaaatc atcccaaact tcccacccca taccctatta ccactgccaa 9901 ttacctgtgg tttcatttac tctaaacctg tgattcctct gaattatttt cattttaaag 9961 aaattgtatt tgttaaatat gtactacaaa cttagtagt

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.