pSIN-EF2-Dse-Puro plasmid

pSIN-EF2-Dse-Puro plasmid

PVT104737

pSIN-EF2-Dse-Puro plasmid Information

Plasmid name: pSIN-EF2-Dse(mouse)-Puro
Alias: NM_172508.3;6030499O08; B130024B19Rik; DS-epi1; Sart2
Gene lenght: 2877
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: EF-1a
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: EF1a-R-F
Reverse primer:
Copy number: High
Induction:

pSIN-EF2-Dse-Puro plasmid Description

pSIN-EF2-Dse-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pSIN-EF2-Dse-Puro plasmid Sequence

1 CGGACCGCCA CTGCCAATTA CCTGTGGTTT CATTTACTCT AAACCTGTGA TTCCTCTGAA 61 TTATTTTCAT TTTAAAGAAA TTGTATTTGT TAAATATGTA CTACAAACTT AGTAGTTGGA 121 AGGGCTAATT CACTCCCAAA GAAGACAAGA TATCCTTGAT CTGTGGATCT ACCACACACA 181 AGGCTACTTC CCTGATTAGC AGAACTACAC ACCAGGGCCA GGGGTCAGAT ATCCACTGAC 241 CTTTGGATGG TGCTACAAGC TAGTACCAGT TGAGCCAGAT AAGGTAGAAG AGGCCAATAA 301 AGGAGAGAAC ACCAGCTTGT TACACCCTGT GAGCCTGCAT GGGATGGATG ACCCGGAGAG 361 AGAAGTGTTA GAGTGGAGGT TTGACAGCCG CCTAGCATTT CATCACGTGG CCCGAGAGCT 421 GCATCCGGAG TACTTCAAGA ACTGCTGATA TCGAGCTTGC TACAAGGGAC TTTCCGCTGG 481 GGACTTTCCA GGGAGGCGTG GCCTGGGCGG GACTGGGGAG TGGCGAGCCC TCAGATCCTG 541 CATATAAGCA GCTGCTTTTT GCCTGTACTG GGTCTCTCTG GTTAGACCAG ATCTGAGCCT 601 GGGAGCTCTC TGGCTAACTA GGGAACCCAC TGCTTAAGCC TCAATAAAGC TTGCCTTGAG 661 TGCTTCAAGT AGTGTGTGCC CGTCTGTTGT GTGACTCTGG TAACTAGAGA TCCCTCAGAC 721 CCTTTTAGTC AGTGTGGAAA ATCTCTAGCA GTGGCGCCCG AACAGGGACT TGAAAGCGAA 781 AGGGAAACCA GAGGAGCTCT CTCGACGCAG GACTCGGCTT GCTGAAGCGC GCACGGCAAG 841 AGGCGAGGGG CGGCGACTGG TGAGTACGCC AAAAATTTTG ACTAGCGGAG GCTAGAAGGA 901 GAGAGATGGG TGCGAGAGCG TCAGTATTAA GCGGGGGAGA ATTAGATCGC GATGGGAAAA 961 AATTCGGTTA AGGCCAGGGG GAAAGAAAAA ATATAAATTA AAACATATAG TATGGGCAAG 1021 CAGGGAGCTA GAACGATTCG CAGTTAATCC TGGCCTGTTA GAAACATCAG AAGGCTGTAG 1081 ACAAATACTG GGACAGCTAC AACCATCCCT TCAGACAGGA TCAGAAGAAC TTAGATCATT 1141 ATATAATACA GTAGCAACCC TCTATTGTGT GCATCAAAGG ATAGAGATAA AAGACACCAA 1201 GGAAGCTTTA GACAAGATAG AGGAAGAGCA AAACAAAAGT AAGACCACCG CACAGCAAGC 1261 GGCCGCTGAT CTTCAGACCT GGAGGAGGAG ATATGAGGGA CAATTGGAGA AGTGAATTAT 1321 ATAAATATAA AGTAGTAAAA ATTGAACCAT TAGGAGTAGC ACCCACCAAG GCAAAGAGAA 1381 GAGTGGTGCA GAGAGAAAAA AGAGCAGTGG GAATAGGAGC TTTGTTCCTT GGGTTCTTGG 1441 GAGCAGCAGG AAGCACTATG GGCGCAGCGT CAATGACGCT GACGGTACAG GCCAGACAAT 1501 TATTGTCTGG TATAGTGCAG CAGCAGAACA ATTTGCTGAG GGCTATTGAG GCGCAACAGC 1561 ATCTGTTGCA ACTCACAGTC TGGGGCATCA AGCAGCTCCA GGCAAGAATC CTGGCTGTGG 1621 AAAGATACCT AAAGGATCAA CAGCTCCTGG GGATTTGGGG TTGCTCTGGA AAACTCATTT 1681 GCACCACTGC TGTGCCTTGG AATGCTAGTT GGAGTAATAA ATCTCTGGAA CAGATTTGGA 1741 ATCACACGAC CTGGATGGAG TGGGACAGAG AAATTAACAA TTACACAAGC TTAATACACT 1801 CCTTAATTGA AGAATCGCAA AACCAGCAAG AAAAGAATGA ACAAGAATTA TTGGAATTAG 1861 ATAAATGGGC AAGTTTGTGG AATTGGTTTA ACATAACAAA TTGGCTGTGG TATATAAAAT 1921 TATTCATAAT GATAGTAGGA GGCTTGGTAG GTTTAAGAAT AGTTTTTGCT GTACTTTCTA 1981 TAGTGAATAG AGTTAGGCAG GGATATTCAC CATTATCGTT TCAGACCCAC CTCCCAACCC 2041 CGAGGGGACC CGACAGGCCC GAAGGAATAG AAGAAGAAGG TGGAGAGAGA GACAGAGACA 2101 GATCCATTCG ATTAGTGAAC GGATCTCGAC GGTATCGCCA CAAATGGCAG TATTCATCCA 2161 CAATTTTAAA AGAAAGGGGG GGATTGGGGG GTACAGTGCA GGGGAAAGAA TAGTAGACAT 2221 AATAGCAACA GACATACAAA CTAAAGAATT ACAAAAACAA ATTACAAAAA TTCAAAATTT 2281 TCGGGTTTAT TACAGGGACA GCAGAGATCC ACTTTGGATC GATAAGCTTT GCAAAGATGG 2341 ATAAAGTTTT AAACAGAGAG GAATCTTTGC AGCTAATGGA CCTTCTAGGT CTTGAAAGGA 2401 GTGGGAATTG GCTCCGGTGC CCGTCAGTGG GCAGAGCGCA CATCGCCCAC AGTCCCCGAG 2461 AAGTTGGGGG GAGGGGTCGG CAATTGAACC GGTGCCTAGA GAAGGTGGCG CGGGGTAAAC 2521 TGGGAAAGTG ATGTCGTGTA CTGGCTCCGC CTTTTTCCCG AGGGTGGGGG AGAACCGTAT 2581 ATAAGTGCAG TAGTCGCCGT GAACGTTCTT TTTCGCAACG GGTTTGCCGC CAGAACACAG 2641 GTAAGTGCCG TGTGTGGTTC CCGCGGGCCT GGCCTCTTTA CGGGTTATGG CCCTTGCGTG 2701 CCTTGAATTA CTTCCACCTG GCTGCAGTAC GTGATTCTTG ATCCCGAGCT TCGGGTTGGA 2761 AGTGGGTGGG AGAGTTCGAG GCCTTGCGCT TAAGGAGCCC CTTCGCCTCG TGCTTGAGTT 2821 GAGGCCTGGC CTGGGCGCTG GGGCCGCCGC GTGCGAATCT GGTGGCACCT TCGCGCCTGT 2881 CTCGCTGCTT TCGATAAGTC TCTAGCCATT TAAAATTTTT GATGACCTGC TGCGACGCTT 2941 TTTTTCTGGC AAGATAGTCT TGTAAATGCG GGCCAAGATC TGCACACTGG TATTTCGGTT 3001 TTTGGGGCCG CGGGCGGCGA CGGGGCCCGT GCGTCCCAGC GCACATGTTC GGCGAGGCGG 3061 GGCCTGCGAG CGCGGCCACC GAGAATCGGA CGGGGGTAGT CTCAAGCTGG CCGGCCTGCT 3121 CTGGTGCCTG GCCTCGCGCC GCCGTGTATC GCCCCGCCCT GGGCGGCAAG GCTGGCCCGG 3181 TCGGCACCAG TTGCGTGAGC GGAAAGATGG CCGCTTCCCG GCCCTGCTGC AGGGAGCTCA 3241 AAATGGAGGA CGCGGCGCTC GGGAGAGCGG GCGGGTGAGT CACCCACACA AAGGAAAAGG 3301 GCCTTTCCGT CCTCAGCCGT CGCTTCATGT GACTCCACGG AGTACCGGGC GCCGTCCAGG 3361 CACCTCGATT AGTTCTCGAG CTTTTGGAGT ACGTCGTCTT TAGGTTGGGG GGAGGGGTTT 3421 TATGCGATGG AGTTTCCCCA CACTGAGTGG GTGGAGACTG AAGTTAGGCC AGCTTGGCAC 3481 TTGATGTAAT TCTCCTTGGA ATTTGCCCTT TTTGAGTTTG GATCTTGGTT CATTCTCAAG 3541 CCTCAGACAG TGGTTCAAAG TTTTTTTCTT CCATTTCAGG TGTCGTGAGG AATTGGGATC 3601 CGCCACCatg aggactcata cacggggggc acccagtgtg tttttcatat gtttgctctg 3661 ctgtgtgtca gccttcatca ctgacgagaa cccagaagtc atgattccct tcactaatgc 3721 caactatgac agccacccaa tgttgtactt ctccaggaaa gatgtggcag agctccagct 3781 gagggctgcc agctcccatg agcacattgc agctcggctt acagaggctg tgcataccat 3841 gttgactaac cccttggagt acctccctcc ttgggatccc aaggagtaca gtgcccgctg 3901 gaatgagatc tatggaaaca acctgggtgc cttggcaatg ttctgtgtgc tgtatcctga 3961 gaacactgaa gctcgagaca tggccaaaga ctacatggag aggatggcag cgcagcctag 4021 ttggttggtg aaagatgcgc cttgggatga agttccactt gcccactccc tggtgggttt 4081 cgccactgca tatgacttct tatacaacta cctgagcaag acacagcagg agacattcct 4141 ggaagtgatt gccaatgcct cgggctatat gtatgaaact tcctatagaa gaggatgggg 4201 gttccagtac ttgcacaacc accagcccac caactgcatg gccttactca ctggaagtct 4261 cattctgatg aaccaagggt accttcagga ggcctattta tggaccaagc aagttctaag 4321 catcatggag aagtctctgg tcttgctgcg agaggtgaca gatggctctc tctatgaagg 4381 agttgcatac ggcagctata ccactagatc cctgttccag tacatgtttc ttgttcagag 4441 gcactttgac atcaaccact ttggccatcc atggcttaag caacactttg cctttatgta 4501 taggaccatc ttgccagggt ttcagagaac tgtagccatt gcagattcaa attacaactg 4561 gttttatggc ccagaaagtc aattagtgtt cctggataaa tttgtcatgc gtaacgggag 4621 tggaaactgg ctggctgacc aaatcagaag gaaccgtgta gtggaaggtc cagggacacc 4681 atcgaaaggg cagcgctggt gcacgctaca caccgaattt ctctggtatg atgccagctt 4741 gaaaccagtt ccacctccag attttggtac cccaacattg cattattttg aggactgggg 4801 tgttgtgact tatggaagtg cactacctgc agaaatcaac aggtcattcc tttccttcaa 4861 gtcaggaaaa cttggagggc gtgcaatata tgacattgtt catagaaaca aatataaaga 4921 ctggatcaaa gggtggagaa attttaatgc aggacatgag caccctgacc aaaactcttt 4981 cactttcgcc cccaatggtg tgccgttcat tactgaggcc ctgtatgggc caaagtacac 5041 ctactttaac aacgttctaa tgttttctcc agctgtgtct aagagttgct tttctccctg 5101 ggagggtcag gtcacagaag actgttcatc aaaatggtca aagtataagc atgacctggc 5161 agccagctgt caggggagag tgattgcagc agatgaaaag gatggggttg tttttatccg 5221 aggagaaggc gtaggagcat ataaccccat gctcaacctg aagcacattc aacggaatct 5281 catcctcctc cacccacagc ttctccttct tgtagaccag atccacctgg gagaggagag 5341 cccgctggag acagcagcaa gtttcttcca caatgtggat gtcccttttg aggagacagt 5401 agtagatggg gtccatgggg ctctcatccg gcagcgagac ggtctctata aaatgtactg 5461 gatggatgat accggttata gtgaaaaagc aaactttgcc tcagtgatgt atcctcgggg 5521 ttatccctac aacgggacaa attatgtgaa tgtcaccatg catcttcgaa gccctatcac 5581 cagggcagct tacctcttca tagggccatc tgtggatgtc cagagcttca gcatccatgg 5641 agaccctcag cggctggatg tgttcatagc taccagtgaa cacgcctatg caacttacct 5701 gtggacaggt gagaacacag ggcattctgc tttcgcacag gtcattgcag atcatcagaa 5761 aattctgttt gaccagagtt cagccatcaa aagcaccgct gtccctgaag tgaaagatta 5821 tgctgctatc gtggagcaga atctgcagca ttttaagccc gtgttccagc tgcttgagaa 5881 gcagatccta tctcgagtcc agaacacagc tagctttaga aagactgccg agcgcctact 5941 gaggttttca gataagagac agacagagga agccattgac agaatttttg ctatatccca 6001 gcaacagcgg cagcagcgag gcaagtcgaa gaaaagccga aaggcgggta agcattacaa 6061 gtttgtggat gcggttcctg atattttcgc acagattgaa gttaatgaga agaagattcg 6121 acagaaagct caggttttag ctcagagaga acagcccata gacgaagatg aagaaatgaa 6181 agaccttttg gattttgctg atgtcacata tgagaagcat aaaaacgagg gttccgtgaa 6241 aggtggcttt ggacaggtgc ggatggtgac aagtcacaac agagcacctt cactgtcagc 6301 ctcctacacc agactcttct tgatcctcaa cattgccatc ttctttgtca tgctggcaat 6361 gcaactgact tatttccaga gggctcagag cctccatggc caaaggtgtc tttatgcggt 6421 cctcctaata gatagctgtg tcttactgtg gttgtattcc tcctgttccc agtctcagtg 6481 ctaaGAATTC TTCGAACATA TGAAGCTTGC TAGCACTAGT GATCCGCATG CATCTAGGGC 6541 GGCCAATTCC GCCCCTCTCC CCCCCCCCCC TCTCCCTCCC CCCCCCCTAA CGTTACTGGC 6601 CGAAGCCGCT TGGAATAAGG CCGGTGTGCG TTTGTCTATA TGTTATTTTC CACCATATTG 6661 CCGTCTTTTG GCAATGTGAG GGCCCGGAAA CCTGGCCCTG TCTTCTTGAC GAGCATTCCT 6721 AGGGGTCTTT CCCCTCTCGC CAAAGGAATG CAAGGTCTGT TGAATGTCGT GAAGGAAGCA 6781 GTTCCTCTGG AAGCTTCTTG AAGACAAACA ACGTCTGTAG CGACCCTTTG CAGGCAGCGG 6841 AACCCCCCAC CTGGCGACAG GTGCCTCTGC GGCCAAAAGC CACGTGTATA AGATACACCT 6901 GCAAAGGCGG CACAACCCCA GTGCCACGTT GTGAGTTGGA TAGTTGTGGA AAGAGTCAAA 6961 TGGCTCTCCT CAAGCGTATT CAACAAGGGG CTGAAGGATG CCCAGAAGGT ACCCCATTGT 7021 ATGGGATCTG ATCTGGGGCC TCGGTGCACA TGCTTTACAT GTGTTTAGTC GAGGTTAAAA 7081 AAACGTCTAG GCCCCCCGAA CCACGGGGAC GTGGTTTTCC TTTGAAAAAC ACGATGATAA 7141 GCTTGCCACA ACCCACAAGG AGACGACCTT CCATGACCGA GTACAAGCCC ACGGTGCGCC 7201 TCGCCACCCG CGACGACGTC CCCCGGGCCG TACGCACCCT CGCCGCCGCG TTCGCCGACT 7261 ACCCCGCCAC GCGCCACACC GTCGACCCGG ACCGCCACAT CGAGCGGGTC ACCGAGCTGC 7321 AAGAACTCTT CCTCACGCGC GTCGGGCTCG ACATCGGCAA GGTGTGGGTC GCGGACGACG 7381 GCGCCGCGGT GGCGGTCTGG ACCACGCCGG AGAGCGTCGA AGCGGGGGCG GTGTTCGCCG 7441 AGATCGGCCC GCGCATGGCC GAGTTGAGCG GTTCCCGGCT GGCCGCGCAG CAACAGATGG 7501 AAGGCCTCCT GGCGCCGCAC CGGCCCAAGG AGCCCGCGTG GTTCCTGGCC ACCGTCGGCG 7561 TCTCGCCCGA CCACCAGGGC AAGGGTCTGG GCAGCGCCGT CGTGCTCCCC GGAGTGGAGG 7621 CGGCCGAGCG CGCCGGGGTG CCCGCCTTCC TGGAGACCTC CGCGCCCCGC AACCTCCCCT 7681 TCTACGAGCG GCTCGGCTTC ACCGTCACCG CCGACGTCGA GGTGCCCGAA GGACCGCGCA 7741 CCTGGTGCAT GACCCGCAAG CCCGGTGCCT GATAATAGGC GGCCGCTCGA GACCTAGAAA 7801 AACATGGAGC AATCACAAGT AGCAATACAG CAGCTACCAA TGCTGATTGT GCCTGGCTAG 7861 AAGCACAAGA GGAGGAGGAG GTGGGTTTTC CAGTCACACC TCAGGTACCT TTAAGACCAA 7921 TGACTTACAA GGCAGCTGTA GATCTTAGCC ACTTTTTAAA AGAAAAGGGG GGACTGGAAG 7981 GGCTAATTCA CTCCCAACGA AGACAAGATC TGCTTTTTGC TTGTACTGGG TCTCTCTGGT 8041 TAGACCAGAT CTGAGCCTGG GAGCTCTCTG GCTAACTAGG GAACCCACTG CTTAAGCCTC 8101 AATAAAGCTT GCCTTGAGTG CTTCAAGTAG TGTGTGCCCG TCTGTTGTGT GACTCTGGTA 8161 ACTAGAGATC CCTCAGACCC TTTTAGTCAG TGTGGAAAAT CTCTAGCAGT AGTAGTTCAT 8221 GTCATCTTAT TATTCAGTAT TTATAACTTG CAAAGAAATG AATATCAGAG AGTGAGAGGC 8281 CTTGACATTA TAATAGATTT AGCAGGAATT GAACTAGGAG TGGAGCACAC AGGCAAAGTT 8341 CTAGAGCTCG CTGATCAGCC TCGACTGTGC CTTCTAGTTG CCAGCCATCT GTTGTTTGCC 8401 CCTCCCCCGT GCCTTCCTTG ACCCTGGAAG GTGCCACTCC CACTGTCCTT TCCTAATAAA 8461 ATGAGGAAAT TGCATCGCAT TGTCTGAGTA GGTGTCATTC TATTCTGGGG GGTGGGGGTG 8521 GGGCAGGACA GCAAGGGGGG AGGATTGGGA AGACAATAGC AGGCATGCTG GGGATGCGGT 8581 GGGCTCTATG GCTTCTGAGG CGGAAAGAAC CAGCTGGGGG CGCGCCCCTC GAGGCCGCCA 8641 TGGTCATAGC TGTTTGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT 8701 TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ACGAGACAAT AACCCTGATA 8761 AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT 8821 TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA 8881 AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA 8941 CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT 9001 TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTATTGAC GCCGGGCAAG AGCAACTCGG 9061 TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA 9121 TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA 9181 CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT 9241 GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC 9301 CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGTAGCA ATGGCAACAA CGTTGCGCAA 9361 ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA 9421 GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC 9481 TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA 9541 TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA 9601 ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA 9661 CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT 9721 CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT 9781 CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT 9841 GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC 9901 GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC 9961 AAATACTGTT CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC 10021 GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC 10081 GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGCGCAGCG GTCGGGCTGA 10141 ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC 10201 CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT 10261 CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC 10321 TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA 10381 TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC 10441 TGGCCTTTTG CA

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.