pLV3-U6-GOT2-sgRNA1-sgRNA2-Cas

pLV3-U6-GOT2-sgRNA1-sgRNA2-Cas9-EGFP-Puro plasmid

PVT104624

pLV3-U6-GOT2-sgRNA1-sgRNA2-Cas9-EGFP-Puro plasmid Information

Plasmid name: pLV3-U6-GOT2(human)-sgRNA1-sgRNA2-Cas9-EGFP-Puro
Alias: GGACGCGGGTCCACTCCCGT AACAGCGAAGTCTTGAAGAG
Gene lenght:
Host: Mammalian cells,lentivirus
Use(s): Gene editing
Fragment type: CRISPR
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: U6
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer:
Reverse primer: EF1a-R(Hairpin structure)
Copy number: High
Induction:

pLV3-U6-GOT2-sgRNA1-sgRNA2-Cas9-EGFP-Puro plasmid Description

pLV3-U6-GOT2-sgRNA1-sgRNA2-Cas9-EGFP-Puro This is a plasmid suitable for gene editing in mammalian cells, and it can also be used for lentivirus packaging.

pLV3-U6-GOT2-sgRNA1-sgRNA2-Cas9-EGFP-Puro plasmid Sequence

1 CAGGGACAGC AGAGATCCAG TTTGGTTAAT TAAGGTACCT TTTGCTGGCC TTTTGCTCAC 61 ATGCGCCATT CTCGAGCATG CGCCATTCTC GAGCATGCTC GAGAATGGCG CATGTGAGGG 121 CCTATTTCCC ATGATTCCTT CATATTTGCA TATACGATAC AAGGCTGTTA GAGAGATAAT 181 TGGAATTAAT TTGACTGTAA ACACAAAGAT ATTAGTACAA AATACGTGAC GTAGAAAGTA 241 ATAATTTCTT GGGTAGTTTG CAGTTTTAAA ATTATGTTTT AAAATGGACT ATCATATGCT 301 TACCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAAA 361 CACCGGGGAC GCGGGTCCAC TCCCGTGTTT TAGAGCTAGA AATAGCAAGT TAAAATAAGG 421 CTAGTCCGTT ATCAACTTGA AAAAGTGGCA CCGAGTCGGT GCTTTTTTGT TTTAGAGCTA 481 GAAATAGCAA GTTAAAATAA GGCTAGTCCG TTTTTAGCGC GTGCGCCAAT TCTGCAGACA 541 AATGGCTCTA GAGAGGGCCT ATTTCCCATG ATTCCTTCAT ATTTGCATAT ACGATACAAG 601 GCTGTTAGAG AGATAATTGG AATTAATTTG ACTGTAAACA CAAAGATATT AGTACAAAAT 661 ACGTGACGTA GAAAGTAATA ATTTCTTGGG TAGTTTGCAG TTTTAAAATT ATGTTTTAAA 721 ATGGACTATC ATATGCTTAC CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC 781 TTGTGGAAAG GACGAAACAC CgAACAGCGA AGTCTTGAAG AGGTTTTAGA GCTAGAAATA 841 GCAAGTTAAA ATAAGGCTAG TCCGTTATCA ACTTGAAAAA GTGGCACCGA GTCGGTGCTT 901 TTTTGTTTTA GAGCTAGAAA TAGCAAGTTA AAATAAGGCT AGTCCGTTTT TAGCGCGTGC 961 GCCAATTCTG CAGACAAATG GGGTACCCGT TACATAACTT ACGGAATTCG CTAGCTAGGT 1021 CTTGAAAGGA GTGGGAATTG GCTCCGGTGC CCGTCAGTGG GCAGAGCGCA CATCGCCCAC 1081 AGTCCCCGAG AAGTTGGGGG GAGGGGTCGG CAATTGATCC GGTGCCTAGA GAAGGTGGCG 1141 CGGGGTAAAC TGGGAAAGTG ATGTCGTGTA CTGGCTCCGC CTTTTTCCCG AGGGTGGGGG 1201 AGAACCGTAT ATAAGTGCAG TAGTCGCCGT GAACGTTCTT TTTCGCAACG GGTTTGCCGC 1261 CAGAACACAG GACCGGTTCT AGAGCGCTGC CACCATGGAC AAGAAGTACA GCATCGGCCT 1321 GGACATCGGC ACCAACTCTG TGGGCTGGGC CGTGATCACC GACGAGTACA AGGTGCCCAG 1381 CAAGAAATTC AAGGTGCTGG GCAACACCGA CCGGCACAGC ATCAAGAAGA ACCTGATCGG 1441 AGCCCTGCTG TTCGACAGCG GCGAAACAGC CGAGGCCACC CGGCTGAAGA GAACCGCCAG 1501 AAGAAGATAC ACCAGACGGA AGAACCGGAT CTGCTATCTG CAAGAGATCT TCAGCAACGA 1561 GATGGCCAAG GTGGACGACA GCTTCTTCCA CAGACTGGAA GAGTCCTTCC TGGTGGAAGA 1621 GGATAAGAAG CACGAGCGGC ACCCCATCTT CGGCAACATC GTGGACGAGG TGGCCTACCA 1681 CGAGAAGTAC CCCACCATCT ACCACCTGAG AAAGAAACTG GTGGACAGCA CCGACAAGGC 1741 CGACCTGCGG CTGATCTATC TGGCCCTGGC CCACATGATC AAGTTCCGGG GCCACTTCCT 1801 GATCGAGGGC GACCTGAACC CCGACAACAG CGACGTGGAC AAGCTGTTCA TCCAGCTGGT 1861 GCAGACCTAC AACCAGCTGT TCGAGGAAAA CCCCATCAAC GCCAGCGGCG TGGACGCCAA 1921 GGCCATCCTG TCTGCCAGAC TGAGCAAGAG CAGACGGCTG GAAAATCTGA TCGCCCAGCT 1981 GCCCGGCGAG AAGAAGAATG GCCTGTTCGG AAACCTGATT GCCCTGAGCC TGGGCCTGAC 2041 CCCCAACTTC AAGAGCAACT TCGACCTGGC CGAGGATGCC AAACTGCAGC TGAGCAAGGA 2101 CACCTACGAC GACGACCTGG ACAACCTGCT GGCCCAGATC GGCGACCAGT ACGCCGACCT 2161 GTTTCTGGCC GCCAAGAACC TGTCCGACGC CATCCTGCTG AGCGACATCC TGAGAGTGAA 2221 CACCGAGATC ACCAAGGCCC CCCTGAGCGC CTCTATGATC AAGAGATACG ACGAGCACCA 2281 CCAGGACCTG ACCCTGCTGA AAGCTCTCGT GCGGCAGCAG CTGCCTGAGA AGTACAAAGA 2341 GATTTTCTTC GACCAGAGCA AGAACGGCTA CGCCGGCTAC ATTGACGGCG GAGCCAGCCA 2401 GGAAGAGTTC TACAAGTTCA TCAAGCCCAT CCTGGAAAAG ATGGACGGCA CCGAGGAACT 2461 GCTCGTGAAG CTGAACAGAG AGGACCTGCT GCGGAAGCAG CGGACCTTCG ACAACGGCAG 2521 CATCCCCCAC CAGATCCACC TGGGAGAGCT GCACGCCATT CTGCGGCGGC AGGAAGATTT 2581 TTACCCATTC CTGAAGGACA ACCGGGAAAA GATCGAGAAG ATCCTGACCT TCCGCATCCC 2641 CTACTACGTG GGCCCTCTGG CCAGGGGAAA CAGCAGATTC GCCTGGATGA CCAGAAAGAG 2701 CGAGGAAACC ATCACCCCCT GGAACTTCGA GGAAGTGGTG GACAAGGGCG CTTCCGCCCA 2761 GAGCTTCATC GAGCGGATGA CCAACTTCGA TAAGAACCTG CCCAACGAGA AGGTGCTGCC 2821 CAAGCACAGC CTGCTGTACG AGTACTTCAC CGTGTATAAC GAGCTGACCA AAGTGAAATA 2881 CGTGACCGAG GGAATGAGAA AGCCCGCCTT CCTGAGCGGC GAGCAGAAAA AGGCCATCGT 2941 GGACCTGCTG TTCAAGACCA ACCGGAAAGT GACCGTGAAG CAGCTGAAAG AGGACTACTT 3001 CAAGAAAATC GAGTGCTTCG ACTCCGTGGA AATCTCCGGC GTGGAAGATC GGTTCAACGC 3061 CTCCCTGGGC ACATACCACG ATCTGCTGAA AATTATCAAG GACAAGGACT TCCTGGACAA 3121 TGAGGAAAAC GAGGACATTC TGGAAGATAT CGTGCTGACC CTGACACTGT TTGAGGACAG 3181 AGAGATGATC GAGGAACGGC TGAAAACCTA TGCCCACCTG TTCGACGACA AAGTGATGAA 3241 GCAGCTGAAG CGGCGGAGAT ACACCGGCTG GGGCAGGCTG AGCCGGAAGC TGATCAACGG 3301 CATCCGGGAC AAGCAGTCCG GCAAGACAAT CCTGGATTTC CTGAAGTCCG ACGGCTTCGC 3361 CAACAGAAAC TTCATGCAGC TGATCCACGA CGACAGCCTG ACCTTTAAAG AGGACATCCA 3421 GAAAGCCCAG GTGTCCGGCC AGGGCGATAG CCTGCACGAG CACATTGCCA ATCTGGCCGG 3481 CAGCCCCGCC ATTAAGAAGG GCATCCTGCA GACAGTGAAG GTGGTGGACG AGCTCGTGAA 3541 AGTGATGGGC CGGCACAAGC CCGAGAACAT CGTGATCGAA ATGGCCAGAG AGAACCAGAC 3601 CACCCAGAAG GGACAGAAGA ACAGCCGCGA GAGAATGAAG CGGATCGAAG AGGGCATCAA 3661 AGAGCTGGGC AGCCAGATCC TGAAAGAACA CCCCGTGGAA AACACCCAGC TGCAGAACGA 3721 GAAGCTGTAC CTGTACTACC TGCAGAATGG GCGGGATATG TACGTGGACC AGGAACTGGA 3781 CATCAACCGG CTGTCCGACT ACGATGTGGA CCATATCGTG CCTCAGAGCT TTCTGAAGGA 3841 CGACTCCATC GACAACAAGG TGCTGACCAG AAGCGACAAG AACCGGGGCA AGAGCGACAA 3901 CGTGCCCTCC GAAGAGGTCG TGAAGAAGAT GAAGAACTAC TGGCGGCAGC TGCTGAACGC 3961 CAAGCTGATT ACCCAGAGAA AGTTCGACAA TCTGACCAAG GCCGAGAGAG GCGGCCTGAG 4021 CGAACTGGAT AAGGCCGGCT TCATCAAGAG ACAGCTGGTG GAAACCCGGC AGATCACAAA 4081 GCACGTGGCA CAGATCCTGG ACTCCCGGAT GAACACTAAG TACGACGAGA ATGACAAGCT 4141 GATCCGGGAA GTGAAAGTGA TCACCCTGAA GTCCAAGCTG GTGTCCGATT TCCGGAAGGA 4201 TTTCCAGTTT TACAAAGTGC GCGAGATCAA CAACTACCAC CACGCCCACG ACGCCTACCT 4261 GAACGCCGTC GTGGGAACCG CCCTGATCAA AAAGTACCCT AAGCTGGAAA GCGAGTTCGT 4321 GTACGGCGAC TACAAGGTGT ACGACGTGCG GAAGATGATC GCCAAGAGCG AGCAGGAAAT 4381 CGGCAAGGCT ACCGCCAAGT ACTTCTTCTA CAGCAACATC ATGAACTTTT TCAAGACCGA 4441 GATTACCCTG GCCAACGGCG AGATCCGGAA GCGGCCTCTG ATCGAGACAA ACGGCGAAAC 4501 CGGGGAGATC GTGTGGGATA AGGGCCGGGA TTTTGCCACC GTGCGGAAAG TGCTGAGCAT 4561 GCCCCAAGTG AATATCGTGA AAAAGACCGA GGTGCAGACA GGCGGCTTCA GCAAAGAGTC 4621 TATCCTGCCC AAGAGGAACA GCGATAAGCT GATCGCCAGA AAGAAGGACT GGGACCCTAA 4681 GAAGTACGGC GGCTTCGACA GCCCCACCGT GGCCTATTCT GTGCTGGTGG TGGCCAAAGT 4741 GGAAAAGGGC AAGTCCAAGA AACTGAAGAG TGTGAAAGAG CTGCTGGGGA TCACCATCAT 4801 GGAAAGAAGC AGCTTCGAGA AGAATCCCAT CGACTTTCTG GAAGCCAAGG GCTACAAAGA 4861 AGTGAAAAAG GACCTGATCA TCAAGCTGCC TAAGTACTCC CTGTTCGAGC TGGAAAACGG 4921 CCGGAAGAGA ATGCTGGCCT CTGCCGGCGA ACTGCAGAAG GGAAACGAAC TGGCCCTGCC 4981 CTCCAAATAT GTGAACTTCC TGTACCTGGC CAGCCACTAT GAGAAGCTGA AGGGCTCCCC 5041 CGAGGATAAT GAGCAGAAAC AGCTGTTTGT GGAACAGCAC AAGCACTACC TGGACGAGAT 5101 CATCGAGCAG ATCAGCGAGT TCTCCAAGAG AGTGATCCTG GCCGACGCTA ATCTGGACAA 5161 AGTGCTGTCC GCCTACAACA AGCACCGGGA TAAGCCCATC AGAGAGCAGG CCGAGAATAT 5221 CATCCACCTG TTTACCCTGA CCAATCTGGG AGCCCCTGCC GCCTTCAAGT ACTTTGACAC 5281 CACCATCGAC CGGAAGAGGT ACACCAGCAC CAAAGAGGTG CTGGACGCCA CCCTGATCCA 5341 CCAGAGCATC ACCGGCCTGT ACGAGACACG GATCGACCTG TCTCAGCTGG GAGGCGACAA 5401 GCGACCTGCC GCCACAAAGA AGGCTGGACA GGCTAAGAAG AAGAAAGATT ACAAAGACGA 5461 TGACGATAAG GGATCCGGCG CAACAAACTT CTCTCTGCTG AAACAAGCCG GAGATGTCGA 5521 AGAGAATCCT GGACCGATGG TGTCTAAGGG CGAAGAGCTG TTCACCGGGG TGGTGCCCAT 5581 CCTGGTCGAG CTGGACGGCG ACGTAAACGG CCACAAGTTC AGCGTGTCCG GCGAGGGCGA 5641 GGGCGATGCC ACCTACGGCA AGCTGACCCT GAAGTTCATC TGCACCACCG GCAAGCTGCC 5701 CGTGCCCTGG CCCACCCTCG TGACCACCCT GACCTACGGC GTGCAGTGCT TCAGCCGCTA 5761 CCCCGACCAC ATGAAGCAGC ACGACTTCTT CAAGTCCGCC ATGCCCGAAG GCTACGTCCA 5821 GGAGCGCACC ATCTTCTTCA AGGACGACGG CAACTACAAG ACCCGCGCCG AGGTGAAGTT 5881 CGAGGGCGAC ACCCTGGTGA ACCGCATCGA GCTGAAGGGC ATCGACTTCA AGGAGGACGG 5941 CAACATCCTG GGGCACAAGC TGGAGTACAA CTACAACAGC CACAACGTCT ATATCATGGC 6001 CGACAAGCAG AAGAACGGCA TCAAGGTGAA CTTCAAGATC CGCCACAACA TCGAGGACGG 6061 CAGCGTGCAG CTCGCCGACC ACTACCAGCA GAACACCCCC ATCGGCGACG GCCCCGTGCT 6121 GCTGCCCGAC AACCACTACC TGAGCACCCA GTCCGCCCTG AGCAAAGACC CCAACGAGAA 6181 GCGCGATCAC ATGGTCCTGC TGGAGTTCGT GACCGCCGCC GGGATCACTC TCGGCATGGA 6241 CGAGCTGTAC AAGgagggca gaggaagtct tctaacatgc ggtgacgtgg aggagaatcc 6301 cggccctATG ACCGAGTACA AGCCCACGGT GCGCCTCGCC ACCCGCGACG ACGTCCCCAG 6361 GGCCGTACGC ACCCTCGCCG CCGCGTTCGC CGACTACCCC GCCACGCGCC ACACCGTCGA 6421 TCCGGACCGC CACATCGAGC GGGTCACCGA GCTGCAAGAA CTCTTCCTCA CGCGCGTCGG 6481 GCTCGACATC GGCAAGGTGT GGGTCGCGGA CGACGGCGCC GCGGTGGCGG TCTGGACCAC 6541 GCCGGAGAGC GTCGAAGCGG GGGCGGTGTT CGCCGAGATC GGCCCGCGCA TGGCCGAGTT 6601 GAGCGGTTCC CGGCTGGCCG CGCAGCAACA GATGGAAGGC CTCCTGGCGC CGCACCGGCC 6661 CAAGGAGCCC GCGTGGTTCC TGGCCACCGT CGGAGTCTCG CCCGACCACC AGGGCAAGGG 6721 TCTGGGCAGC GCCGTCGTGC TCCCCGGAGT GGAGGCGGCC GAGCGCGCCG GGGTGCCCGC 6781 CTTCCTGGAG ACCTCCGCGC CCCGCAACCT CCCCTTCTAC GAGCGGCTCG GCTTCACCGT 6841 CACCGCCGAC GTCGAGGTGC CCGAAGGACC GCGCACCTGG TGCATGACCC GCAAGCCCGG 6901 TGCCTGActc gagTACGCGT TAAGTCGACA ATCAACCTCT GGATTACAAA ATTTGTGAAA 6961 GATTGACTGG TATTCTTAAC TATGTTGCTC CTTTTACGCT ATGTGGATAC GCTGCTTTAA 7021 TGCCTTTGTA TCATGCTATT GCTTCCCGTA TGGCTTTCAT TTTCTCCTCC TTGTATAAAT 7081 CCTGGTTGCT GTCTCTTTAT GAGGAGTTGT GGCCCGTTGT CAGGCAACGT GGCGTGGTGT 7141 GCACTGTGTT TGCTGACGCA ACCCCCACTG GTTGGGGCAT TGCCACCACC TGTCAGCTCC 7201 TTTCCGGGAC TTTCGCTTTC CCCCTCCCTA TTGCCACGGC GGAACTCATC GCCGCCTGCC 7261 TTGCCCGCTG CTGGACAGGG GCTCGGCTGT TGGGCACTGA CAATTCCGTG GTGTTGTCGG 7321 GGAAATCATC GTCCTTTCCT TGGCTGCTCG CCTGTGTTGC CACCTGGATT CTGCGCGGGA 7381 CGTCCTTCTG CTACGTCCCT TCGGCCCTCA ATCCAGCGGA CCTTCCTTCC CGCGGCCTGC 7441 TGCCGGCTCT GCGGCCTCTT CCGCGTCTTC GCCTTCGCCC TCAGACGAGT CGGATCTCCC 7501 TTTGGGCCGC CTCCCCGCGT CGACTTTAAG ACCAATGACT TACAAGGCAG CTGTAGATCT 7561 TAGCCACTTT TTAAAAGAAA AGGGGGGACT GGAAGGGCTA ATTCACTCCC AACGAAGACA 7621 AGATCTGCTT TTTGCTTGTA CTGGGTCTCT CTGGTTAGAC CAGATCTGAG CCTGGGAGCT 7681 CTCTGGCTAA CTAGGGAACC CACTGCTTAA GCCTCAATAA AGCTTGCCTT GAGTGCTTCA 7741 AGTAGTGTGT GCCCGTCTGT TGTGTGACTC TGGTAACTAG AGATCCCTCA GACCCTTTTA 7801 GTCAGTGTGG AAAATCTCTA GCAGGGCCCG TTTAAACCCG CTGATCAGCC TCGACTGTGC 7861 CTTCTAGTTG CCAGCCATCT GTTGTTTGCC CCTCCCCCGT GCCTTCCTTG ACCCTGGAAG 7921 GTGCCACTCC CACTGTCCTT TCCTAATAAA ATGAGGAAAT TGCATCGCAT TGTCTGAGTA 7981 GGTGTCATTC TATTCTGGGG GGTGGGGTGG GGCAGGACAG CAAGGGGGAG GATTGGGAAG 8041 ACAATAGCAG GCATGCTGGG GATGCGGTGG GCTCTATGGC TTCTGAGGCG GAAAGAACCA 8101 GCTGGGGCTC TAGGGGGTAT CCCCACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG 8161 TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG 8221 CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG 8281 GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT 8341 AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT 8401 TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA 8461 TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA 8521 ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTAATTCTG TGGAATCACG TGCTACGAGA 8581 TTTCGATTCC ACCGCCGCCT TCTATGAAAG GTTGGGCTTC GGAATCGTTT TCCGGGACGC 8641 CGGCTGGATG ATCCTCCAGC GCGGGGATCT CATGCTGGAG TTCTTCGCCC ACCCCAACTT 8701 GTTTATTGCA GCTTATAATG GTTACAAATA AAGCAATAGC ATCACAAATT TCACAAATAA 8761 AGCATTTTTT TCACTGCATT CTAGTTGTGG TTTGTCCAAA CTCATCAATG TATCTTATCA 8821 TGTCTGTATA CCGTCGACCT CTAGCTAGAG CTTGGCGTAA TCATGGTCAT AGCTGTTTCC 8881 TGTGTGAAAT TGTTATCCGC TCACAATTCC ACACAACATA CGAGCCGGAA GCATAAAGTG 8941 TAAAGCCTGG GGTGCCTAAT GAGTGAGCTA ACTCACATTA ATTGCGTTGC GCTCACTGCC 9001 CGCTTTCCAG TCGGGAAACC TGTCGTGCCA GCTGCATTAA TGAATCGGCC AACGCGCGGG 9061 GAGAGGCGGT TTGCGTATTG GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC 9121 GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 9181 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 9241 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 9301 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 9361 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 9421 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 9481 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 9541 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 9601 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 9661 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 9721 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 9781 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 9841 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 9901 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 9961 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC 10021 TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC 10081 ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC 10141 TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC 10201 AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC 10261 CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT 10321 GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC 10381 TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA 10441 AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT 10501 ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG 10561 CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC 10621 GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA 10681 AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT 10741 GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT 10801 CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG 10861 GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA 10921 TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT 10981 AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC GTCGACGGAT CGGGAGATCT 11041 CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG 11101 TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT GCGCGAGCAA AATTTAAGCT 11161 ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC TGCTTAGGGT TAGGCGTTTT 11221 GCGCTGCTTC GCGATGTACG GGCCAGATAT ACGCGTTGAC ATTGATTATT GACTAGTTAT 11281 TAATAGTAAT CAATTACGGG GTCATTAGTT CATAGCCCAT ATATGGAGTT CCGCGTTACA 11341 TAACTTACGG TAAATGGCCC GCCTGGCTGA CCGCCCAACG ACCCCCGCCC ATTGACGTCA 11401 ATAATGACGT ATGTTCCCAT AGTAACGCCA ATAGGGACTT TCCATTGACG TCAATGGGTG 11461 GAGTATTTAC GGTAAACTGC CCACTTGGCA GTACATCAAG TGTATCATAT GCCAAGTACG 11521 CCCCCTATTG ACGTCAATGA CGGTAAATGG CCCGCCTGGC ATTATGCCCA GTACATGACC 11581 TTATGGGACT TTCCTACTTG GCAGTACATC TACGTATTAG TCATCGCTAT TACCATGGTG 11641 ATGCGGTTTT GGCAGTACAT CAATGGGCGT GGATAGCGGT TTGACTCACG GGGATTTCCA 11701 AGTCTCCACC CCATTGACGT CAATGGGAGT TTGTTTTGGC ACCAAAATCA ACGGGACTTT 11761 CCAAAATGTC GTAACAACTC CGCCCCATTG ACGCAAATGG GCGGTAGGCG TGTACGGTGG 11821 GAGGTCTATA TAAGCAGCGC GTTTTGCCTG TACTGGGTCT CTCTGGTTAG ACCAGATCTG 11881 AGCCTGGGAG CTCTCTGGCT AACTAGGGAA CCCACTGCTT AAGCCTCAAT AAAGCTTGCC 11941 TTGAGTGCTT CAAGTAGTGT GTGCCCGTCT GTTGTGTGAC TCTGGTAACT AGAGATCCCT 12001 CAGACCCTTT TAGTCAGTGT GGAAAATCTC TAGCAGTGGC GCCCGAACAG GGACTTGAAA 12061 GCGAAAGGGA AACCAGAGGA GCTCTCTCGA CGCAGGACTC GGCTTGCTGA AGCGCGCACG 12121 GCAAGAGGCG AGGGGCGGCG ACTGGTGAGT ACGCCAAAAA TTTTGACTAG CGGAGGCTAG 12181 AAGGAGAGAG ATGGGTGCGA GAGCGTCAGT ATTAAGCGGG GGAGAATTAG ATCGCGATGG 12241 GAAAAAATTC GGTTAAGGCC AGGGGGAAAG AAAAAATATA AATTAAAACA TATAGTATGG 12301 GCAAGCAGGG AGCTAGAACG ATTCGCAGTT AATCCTGGCC TGTTAGAAAC ATCAGAAGGC 12361 TGTAGACAAA TACTGGGACA GCTACAACCA TCCCTTCAGA CAGGATCAGA AGAACTTAGA 12421 TCATTATATA ATACAGTAGC AACCCTCTAT TGTGTGCATC AAAGGATAGA GATAAAAGAC 12481 ACCAAGGAAG CTTTAGACAA GATAGAGGAA GAGCAAAACA AAAGTAAGAC CACCGCACAG 12541 CAAGCGGCCG CTGATCTTCA GACCTGGAGG AGGAGATATG AGGGACAATT GGAGAAGTGA 12601 ATTATATAAA TATAAAGTAG TAAAAATTGA ACCATTAGGA GTAGCACCCA CCAAGGCAAA 12661 GAGAAGAGTG GTGCAGAGAG AAAAAAGAGC AGTGGGAATA GGAGCTTTGT TCCTTGGGTT 12721 CTTGGGAGCA GCAGGAAGCA CTATGGGCGC AGCGTCAATG ACGCTGACGG TACAGGCCAG 12781 ACAATTATTG TCTGGTATAG TGCAGCAGCA GAACAATTTG CTGAGGGCTA TTGAGGCGCA 12841 ACAGCATCTG TTGCAACTCA CAGTCTGGGG CATCAAGCAG CTCCAGGCAA GAATCCTGGC 12901 TGTGGAAAGA TACCTAAAGG ATCAACAGCT CCTGGGGATT TGGGGTTGCT CTGGAAAACT 12961 CATTTGCACC ACTGCTGTGC CTTGGAATGC TAGTTGGAGT AATAAATCTC TGGAACAGAT 13021 TTGGAATCAC ACGACCTGGA TGGAGTGGGA CAGAGAAATT AACAATTACA CAAGCTTAAT 13081 ACACTCCTTA ATTGAAGAAT CGCAAAACCA GCAAGAAAAG AATGAACAAG AATTATTGGA 13141 ATTAGATAAA TGGGCAAGTT TGTGGAATTG GTTTAACATA ACAAATTGGC TGTGGTATAT 13201 AAAATTATTC ATAATGATAG TAGGAGGCTT GGTAGGTTTA AGAATAGTTT TTGCTGTACT 13261 TTCTATAGTG AATAGAGTTA GGCAGGGATA TTCACCATTA TCGTTTCAGA CCCACCTCCC 13321 AACCCCGAGG GGACCCGACA GGCCCGAAGG AATAGAAGAA GAAGGTGGAG AGAGAGACAG 13381 AGACAGATCC ATTCGATTAG TGAACGGATC GGCACTGCGT GCGCCAATTC TGCAGACAAA 13441 TGGCAGTATT CATCCACAAT TTTAAAAGAA AAGGGGGGAT TGGGGGGTAC AGTGCAGGGG 13501 AAAGAATAGT AGACATAATA GCAACAGACA TACAAACTAA AGAATTACAA AAACAAATTA 13561 CAAAAATTCA AAATTTTCGG GTTTATTA

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.