pLV3-CMV-YWHAH-3×FLAG-mCherry-

pLV3-CMV-YWHAH-3×FLAG-mCherry-Puro plasmid

PVT104553

pLV3-CMV-YWHAH-3×FLAG-mCherry-Puro plasmid Information

Plasmid name: pLV3-CMV-YWHAH(human)-3×FLAG-mCherry-Puro
Alias: NM_003405.4;YWHA1
Gene lenght: 738
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Red
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-YWHAH-3×FLAG-mCherry-Puro plasmid Description

pLV3-CMV-YWHAH-3×FLAG-mCherry-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-YWHAH-3×FLAG-mCherry-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc gaattcGCCA CCatggggga ccgggagcag ctgctgcagc gggcgcggct 2341 ggccgagcag gcggagcgct acgacgacat ggcctccgct atgaaggcgg tgacagagct 2401 gaatgaacct ctctccaatg aagatcgaaa tctcctctct gtggcctaca agaatgtggt 2461 tggtgccagg cgatcttcct ggagggtcat tagcagcatt gagcagaaaa ccatggctga 2521 tggaaacgaa aagaaattgg agaaagttaa agcttaccgg gagaagattg agaaggagct 2581 ggagacagtt tgcaatgatg tcctgtctct gcttgacaag ttcctgatca agaactgcaa 2641 tgatttccag tatgagagca aggtgtttta cctgaaaatg aagggtgatt actaccgcta 2701 cttagcagag gtcgcttctg gggagaagaa aaacagtgtg gtcgaagctt ctgaagctgc 2761 ctacaaggaa gcctttgaaa tcagcaaaga gcagatgcaa cccacgcatc ccatccggct 2821 gggcctggcc ctcaacttct ccgtgttcta ctatgagatc cagaatgcac ctgagcaagc 2881 ctgcctctta gccaaacaag ccttcgatga tgccatagct gagctggaca cactaaacga 2941 ggattcctat aaggactcca cgctgatcat gcagttgctg cgagacaacc tcaccctctg 3001 gacgagcgac cagcaggatg aagaagcagg agaaggcaac ggatccGACT ACAAAGACCA 3061 TGACGGTGAT TATAAAGATC ATGACATCGA CTACAAGGAT GACGATGACA AGtagcggcc 3121 gcGaaggatc tgcgatcgct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt 3181 ccccgagaag ttggggggag gggtcggcaa ttgaacgggt gcctagagaa ggtggcgcgg 3241 ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga 3301 accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag 3361 aacacagctg aagcttcgag gggctcgcat ctctccttca cgcgcccgcc gccctacctg 3421 aggccgccat ccacgccggt tgagtcgcgt tctgccgcct cccgcctgtg gtgcctcctg 3481 aactgcgtcc gccgtctagg taagtttaaa gctcaggtcg agaccgggcc tttgtccggc 3541 gctcccttgg agcctaccta gactcagccg gctctccacg ctttgcctga ccctgcttgc 3601 tcaactctac gtctttgttt cgttttctgt tctgcgccgt tacagatcca agctgtgacc 3661 ggcgcctacg ctagacgcca ccatggtgag caagggcgag gaggataaca tggccatcat 3721 caaggagttc atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga 3781 gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt 3841 gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg 3901 ctccaaggcc tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc 3961 cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac 4021 ccaggactcc tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa 4081 cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga 4141 gcggatgtac cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa 4201 ggacggcggc cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca 4261 gctgcccggc gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta 4321 caccatcgtg gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga 4381 gctgtacaag ggatctcgcg agggcagagg aagtcttcta acatgcggtg acgtggagga 4441 gaatcccggc cctatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt 4501 ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac 4561 cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg 4621 cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg 4681 gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc 4741 cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca 4801 ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg 4861 caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt 4921 gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt 4981 caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa 5041 gcccggtgcc tgaaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct 5101 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc 5161 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct 5221 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga 5281 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc 5341 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac 5401 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt 5461 tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt 5521 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc 5581 tcttccgcgt ctccgccttc gccctcagac gagtcggatc tccctttggc cgcctccccg 5641 cctggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa 5701 agaaaagggg ggactggaag ggctaattca ctcccaacga aaataagatc tgctttttgc 5761 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg 5821 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg 5881 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat 5941 ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg 6001 aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa 6061 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc 6121 caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc 6181 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag 6241 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctagac 6301 ttttgcagag acggcccaaa ttcgtaatca tggtcatagc tgtttcctgt gtgaaattgt 6361 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt 6421 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg 6481 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg 6541 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg 6601 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat 6661 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc 6721 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc 6781 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga 6841 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt 6901 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg 6961 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc 7021 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg 7081 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc 7141 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg 7201 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc 7261 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct 7321 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt 7381 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa 7441 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa 7501 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc 7561 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct 7621 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca 7681 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt 7741 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt 7801 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc 7861 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc 7921 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt 7981 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact 8041 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc 8101 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt 8161 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg 8221 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct 8281 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa 8341 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt 8401 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc 8461 acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc 8521 tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa 8581 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg 8641 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac 8701 tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac 8761 agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc cattcaggct gcgcaactgt 8821 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt 8881 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg 8941 acggccagtg ccaagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.