pLV3-CMV-Mito-Co3(mouse-opt-mo

pLV3-CMV-Mito-Co3(mouse-opt-mouse-3×FLAG-EF1a-CopGFP-Puro plasmid

PVT103876

pLV3-CMV-Mito-Co3(mouse-opt-mouse-3×FLAG-EF1a-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-Mito(mouse)-Co3(mouse-opt-mouse)-3×FLAG-EF1a-CopGFP-Puro
Alias:
Gene lenght: 784
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-Mito-Co3(mouse-opt-mouse-3×FLAG-EF1a-CopGFP-Puro plasmid Description

pLV3-CMV-Mito-Co3(mouse-opt-mouse-3×FLAG-EF1a-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-Mito-Co3(mouse-opt-mouse-3×FLAG-EF1a-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt 1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc 1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga 1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac 1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga 2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg 2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg 2161 caaatgggcg gtaggcgtgt acggtgggag gtttatataa gcagagctcg tttagtgaac 2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagagc 2281 tagcgaattc GCCACCATGT CTGTCCTGAC GCCACTGCTG CTGAGGAGCC TGACCGGCTC 2341 GGCCCGGCGG CTCATGGTGC CGCGGGCTCA GGTCCACTCG AAGATGACCC ACCAGACCCA 2401 CGCCTACCAC ATGGTGAACC CATCCCCATG GCCTCTGACA GGAGCCTTTT CAGCCCTGCT 2461 GCTGACCTCC GGCCTGGTGA TGTGGTTTCA TTACAACTCC ATCACCCTGC TGACCCTGGG 2521 GCTGCTGACA AACATCCTGA CTATGTACCA GTGGTGGAGA GACGTGATCC GCGAGGGCAC 2581 CTACCAGGGC CACCACACAC CCATCGTGCA GAAAGGCCTG CGCTATGGGA TGATCCTGTT 2641 CATTGTGAGC GAAGTGTTCT TCTTCGCAGG CTTCTTCTGG GCATTTTACC ACTCTTCCCT 2701 CGTGCCAACT CACGACCTGG GCGGCTGCTG GCCTCCTACT GGGATCAGCC CACTGAACCC 2761 TCTGGAGGTG CCTCTGCTGA ACACTAGCGT GTTGCTGGCC AGCGGCGTGT CCATCACCTG 2821 GGCCCATCAC TCCCTCATGG AGGGGAAGAG GAACCATATG AATCAGGCCC TGCTGATCAC 2881 CATCATGCTG GGCCTGTACT TTACAATTCT GCAGGCCTCT GAGTACTTTG AGACCTCCTT 2941 CAGCATTTCC GACGGAATTT ACGGCAGCAC ATTCTTTATG GCTACCGGAT TTCACGGCCT 3001 GCACGTGATC ATCGGCAGCA CCTTCCTCAT CGTGTGTCTG CTGAGACAGC TCAAGTTCCA 3061 CTTCACTTCT AAGCACCACT TTGGCTTTGA GGCTGCCGCC TGGTACTGGC ACTTCGTGGA 3121 TGTCGTGTGG CTGTTCCTGT ACGTGAGCAT CTACTGGTGG GGGTCCggat ccGACTACAA 3181 AGACCATGAC GGTGATTATA AAGATCATGA CATCGACTAC AAGGATGACG ATGACAAGTG 3241 AgcggccgcG aaggatctgc gatcgctccg gtgcccgtca gtgggcagag cgcacatcgc 3301 ccacagtccc cgagaagttg gggggagggg tcggcaattg aacgggtgcc tagagaaggt 3361 ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt cccgagggtg 3421 ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg 3481 ccgccagaac acagctgaag cttcgagggg ctcgcatctc tccttcacgc gcccgccgcc 3541 ctacctgagg ccgccatcca cgccggttga gtcgcgttct gccgcctccc gcctgtggtg 3601 cctcctgaac tgcgtccgcc gtctaggtaa gtttaaagct caggtcgaga ccgggccttt 3661 gtccggcgct cccttggagc ctacctagac tcagccggct ctccacgctt tgcctgaccc 3721 tgcttgctca actctacgtc tttgtttcgt tttctgttct gcgccgttac agatccaagc 3781 tgtgaccggc gcctacgcta gacgccacca tggagagcga cgagagcggc ctgcccgcca 3841 tggagatcga gtgccgcatc accggcaccc tgaacggcgt ggagttcgag ctggtgggcg 3901 gcggagaggg cacccccaag cagggccgca tgaccaacaa gatgaagagc accaaaggcg 3961 ccctgacctt cagcccctac ctgctgagcc acgtgatggg ctacggcttc taccacttcg 4021 gcacctaccc cagcggctac gagaacccct tcctgcacgc catcaacaac ggcggctaca 4081 ccaacacccg catcgagaag tacgaggacg gcggcgtgct gcacgtgagc ttcagctacc 4141 gctacgaggc cggccgcgtg atcggcgact tcaaggtggt gggcaccggc ttccccgagg 4201 acagcgtgat cttcaccgac aagatcatcc gcagcaacgc caccgtggag cacctgcacc 4261 ccatgggcga taacgtgctg gtgggcagct tcgcccgcac cttcagcctg cgcgacggcg 4321 gctactacag cttcgtggtg gacagccaca tgcacttcaa gagcgccatc caccccagca 4381 tcctgcagaa cgggggcccc atgttcgcct tccgccgcgt ggaggagctg cacagcaaca 4441 ccgagctggg catcgtggag taccagcacg ccttcaagac ccccatcgcc ttcgccagat 4501 cccgcgctca gtcgtccaat tctgccgtgg acggcaccgc cggacccggc tccaccggat 4561 ctcgcgaggg cagaggaagt cttctaacat gcggtgacgt ggaggagaat cccggcccta 4621 tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac 4681 gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc 4741 gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca 4801 tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga 4861 gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt 4921 cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc 4981 ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca 5041 gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg 5101 agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg 5161 acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgaa 5221 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc 5281 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta 5341 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt 5401 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg 5461 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta 5521 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt 5581 tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg 5641 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca 5701 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtctcc 5761 gccttcgccc tcagacgagt cggatctccc tttggccgcc tccccgcctg gtacctttaa 5821 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac 5881 tggaagggct aattcactcc caacgaaaat aagatctgct ttttgcttgt actgggtctc 5941 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 6001 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 6061 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta 6121 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg 6181 agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt 6241 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg 6301 tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccagtt ccgcccattc 6361 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc 6421 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagactttt gcagagacgg 6481 cccaaattcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat 6541 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag 6601 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg 6661 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc 6721 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc 6781 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa 6841 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt 6901 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg 6961 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg 7021 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag 7081 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc 7141 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa 7201 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg 7261 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc 7321 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac 7381 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg 7441 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt 7501 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt 7561 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa 7621 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga 7681 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt 7741 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg 7801 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga 7861 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga 7921 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg 7981 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc 8041 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc 8101 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca 8161 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac 8221 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg 8281 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc 8341 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg 8401 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac 8461 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat 8521 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata 8581 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa 8641 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg 8701 tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat 8761 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg 8821 tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga 8881 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag 8941 aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc 9001 ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt 9061 aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa 9121 gctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.