pCMV-CLDN18-P2A-mCherry-Neo pl

pCMV-CLDN18-P2A-mCherry-Neo plasmid

PVT103828

pCMV-CLDN18-P2A-mCherry-Neo plasmid Information

Plasmid name: pCMV-CLDN18(human)-P2A-mCherry-Neo
Alias: NM_016369.4;SFTA5; SFTPJ
Gene lenght: 783
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling: Red
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-CLDN18-P2A-mCherry-Neo plasmid Description

pCMV-CLDN18-P2A-mCherry-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-CLDN18-P2A-mCherry-Neo plasmid Sequence

1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta 61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag 121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc 181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga 241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat 301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc 361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct 421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca 481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat 541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg 601 cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg 661 agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc cagcctccgg 721 actctagagc tagcgtttaa acttaagctt ggtaccgagc tcggatccGC CACCatgtcc 781 accaccacat gccaagtggt ggcgttcctc ctgtccatcc tggggctggc cggctgcatc 841 gcggccaccg ggatggacat gtggagcacc caggacctgt acgacaaccc cgtcacctcc 901 gtgttccagt acgaagggct ctggaggagc tgcgtgaggc agagttcagg cttcaccgaa 961 tgcaggccct atttcaccat cctgggactt ccagccatgc tgcaggcagt gcgagccctg 1021 atgatcgtag gcatcgtcct gggtgccatt ggcctcctgg tatccatctt tgccctgaaa 1081 tgcatccgca ttggcagcat ggaggactct gccaaagcca acatgacact gacctccggg 1141 atcatgttca ttgtctcagg tctttgtgca attgctggag tgtctgtgtt tgccaacatg 1201 ctggtgacta acttctggat gtccacagct aacatgtaca ccggcatggg tgggatggtg 1261 cagactgttc agaccaggta cacatttggt gcggctctgt tcgtgggctg ggtcgctgga 1321 ggcctcacac taattggggg tgtgatgatg tgcatcgcct gccggggcct ggcaccagaa 1381 gaaaccaact acaaagccgt ttcttatcat gcctcaggcc acagtgttgc ctacaagcct 1441 ggaggcttca aggccagcac tggctttggg tccaacacca aaaacaagaa gatatacgat 1501 ggaggtgccc gcacagagga cgaggtacaa tcttatcctt ccaagcacga ctatgtggaa 1561 ttcggaagcg gagctactaa cttcagcctg ctgaagcagg ctggcgacgt ggaggagaac 1621 cctggaccta tggtgagcaa gggcgaggag gataacatgg ccatcatcaa ggagttcatg 1681 cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag 1741 ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac caagggtggc 1801 cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc caaggcctac 1861 gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaag 1921 tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc 1981 ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac 2041 ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg gatgtacccc 2101 gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga cggcggccac 2161 tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct gcccggcgcc 2221 tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac catcgtggaa 2281 cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct gtacaagtaa 2341 ctcgagtcta gagggcccct taagggttcg atccctaccg gttagtaatg agtttgatat 2401 ctcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat 2461 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct 2521 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag 2581 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc 2641 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc 2701 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct 2761 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg 2821 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg 2881 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg 2941 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgga 3001 aacgggggag gctaactgaa acacggaagg agacaatacc ggaaggaacc cgcgctatga 3061 cggcaataaa aagacagaat aaaacgcacg ggtgttgggt cgtttgttca taaacgcggg 3121 gttcggtccc agggctggca ctctgtcgat accccaccga gaccccattg gggccaatac 3181 gcccgcgttt cttccttttc cccaccccac cccccaagtt cgggtgaagg cccagggctc 3241 gcagccaacg tcggggcggc aggccctgcc atagcagatc tgcgcagctg gggctctagg 3301 gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc 3361 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc 3421 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcggggcat ccctttaggg 3481 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca 3541 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc 3601 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct 3661 tttgatttat aagggatttt ggggatttcg gcctattggt taaaaaatga gctgatttaa 3721 caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc 3781 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt 3841 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt 3901 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg 3961 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct 4021 ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca 4081 aaaagctccc gggagcttgt atatccattt tcggatctga tcaagagaca ggatgaggat 4141 cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga 4201 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc 4261 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga 4321 atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg 4381 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc 4441 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg 4501 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga 4561 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc 4621 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca 4681 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg 4741 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct 4801 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg 4861 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc 4921 gccttcttga cgagttcttc tgagcgggac tctggggttc gcgaaatgac cgaccaagcg 4981 acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc 5041 ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg 5101 gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat 5161 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc 5221 aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg 5281 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac 5341 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca 5401 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat 5461 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc 5521 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca 5581 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca 5641 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg 5701 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg 5761 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt 5821 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt 5881 tctcaatgct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc 5941 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt 6001 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt 6061 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc 6121 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa 6181 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt 6241 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct 6301 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta 6361 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa 6421 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc 6481 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact 6541 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc 6601 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt 6661 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta 6721 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg 6781 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt 6841 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc 6901 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt 6961 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc 7021 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc 7081 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa 7141 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac 7201 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa 7261 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt 7321 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa 7381 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct 7441 gacgtcgacg gatcgggaga tctcccgatc ccctatggtc gactctcagt acaatctgct 7501 ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt 7561 agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga 7621 atctgcttag g

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.