pCMV-SLC5A7-P2A-mCherry-Neo pl

pCMV-SLC5A7-P2A-mCherry-Neo plasmid

PVT107149

pCMV-SLC5A7-P2A-mCherry-Neo plasmid Information

Plasmid name: pCMV-SLC5A7(human)-P2A-mCherry-Neo
Alias: NM_021815.5;CHT; CHT1; CMS20; DHMNVP; hCHT1; HMN7A;HMND7
Gene lenght: 1740
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling: Red
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-SLC5A7-P2A-mCherry-Neo plasmid Description

pCMV-SLC5A7-P2A-mCherry-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-SLC5A7-P2A-mCherry-Neo plasmid Sequence

1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta 61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag 121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc 181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga 241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat 301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc 361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct 421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca 481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat 541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg 601 cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg 661 agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc cagcctccgg 721 actctagagc tagcgtttaa acttaagctt ggtaccgagc tcggatccaG CCACCatggc 781 tttccatgtg gaaggactga tagctatcat cgtgttctac cttctaattt tgctggttgg 841 aatatgggct gcctggagaa ccaaaaacag tggcagcgca gaagagcgca gcgaagccat 901 catagttggt ggccgagata ttggtttatt ggttggtgga tttaccatga cagctacctg 961 ggtcggagga gggtatatca atggcacagc tgaagcagtt tatgtaccag gttatggcct 1021 agcttgggct caggcaccaa ttggatattc tcttagtctg attttaggtg gcctgttctt 1081 tgcaaaacct atgcgttcaa aggggtatgt gaccatgtta gacccgtttc agcaaatcta 1141 tggaaaacgc atgggcggac tcctgtttat tcctgcactg atgggagaaa tgttctgggc 1201 tgcagcaatt ttctctgctt tgggagccac catcagcgtg atcatcgatg tggatatgca 1261 catttctgtc atcatctctg cactcattgc cactctgtac acactggtgg gagggctcta 1321 ttctgtggcc tacactgatg tcgttcagct cttttgcatt tttgtagggc tgtggatcag 1381 cgtccccttt gcattgtcac atcctgcagt cgcagacatc gggttcactg ctgtgcatgc 1441 caaataccaa aagccgtggc tgggaactgt tgactcatct gaagtctact cttggcttga 1501 tagttttctg ttgttgatgc tgggtggaat cccatggcaa gcatactttc agagggttct 1561 ctcttcttcc tcagccacct atgctcaagt gctgtccttc ctggcagctt tcgggtgcct 1621 ggtgatggcc atcccagcca tactcattgg ggccattgga gcatcaacag actggaacca 1681 gactgcatat gggcttccag atcccaagac tacagaagag gcagacatga ttttaccaat 1741 tgttctgcag tatctctgcc ctgtgtatat ttctttcttt ggtcttggtg cagtttctgc 1801 tgctgttatg tcatcagcag attcttccat cttgtcagca agttccatgt ttgcacggaa 1861 catctaccag ctttccttca gacaaaatgc ttcggacaaa gaaatcgttt gggttatgcg 1921 aatcacagtg tttgtgtttg gagcatctgc aacagccatg gccttgctga cgaaaactgt 1981 gtatgggctc tggtacctca gttctgacct tgtttacatc gttatcttcc cccagctgct 2041 ttgtgtactc tttgttaagg gaaccaacac ctatggggcc gtggcaggtt atgtttctgg 2101 cctcttcctg agaataactg gaggggagcc atatctgtat cttcagccct tgatcttcta 2161 ccctggctat taccctgatg ataatggtat atataatcag aaatttccat ttaaaacact 2221 tgccatggtt acatcattct taaccaacat ttgcatctcc tatctagcca agtatctatt 2281 tgaaagtgga accttgccac ctaaattaga tgtatttgat gctgttgttg caagacacag 2341 tgaagaaaac atggataaga caattcttgt caaaaatgaa aatattaaat tagatgaact 2401 tgcacttgtg aagccacgac agagcatgac cctcagctca actttcacca ataaagaggc 2461 cttccttgat gttgattcca gtccagaagg gtctgggact gaagataatt tacaggaatt 2521 cggaagcgga gctactaact tcagcctgct gaagcaggct ggcgacgtgg aggagaaccc 2581 tggacctatg gtgagcaagg gcgaggagga taacatggcc atcatcaagg agttcatgcg 2641 cttcaaggtg cacatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg 2701 cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca agggtggccc 2761 cctgcccttc gcctgggaca tcctgtcccc tcagttcatg tacggctcca aggcctacgt 2821 gaagcacccc gccgacatcc ccgactactt gaagctgtcc ttccccgagg gcttcaagtg 2881 ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc gtgacccagg actcctccct 2941 gcaggacggc gagttcatct acaaggtgaa gctgcgcggc accaacttcc cctccgacgg 3001 ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc tccgagcgga tgtaccccga 3061 ggacggcgcc ctgaagggcg agatcaagca gaggctgaag ctgaaggacg gcggccacta 3121 cgacgctgag gtcaagacca cctacaaggc caagaagccc gtgcagctgc ccggcgccta 3181 caacgtcaac atcaagttgg acatcacctc ccacaacgag gactacacca tcgtggaaca 3241 gtacgaacgc gccgagggcc gccactccac cggcggcatg gacgagctgt acaagtaact 3301 cgagtctaga gggcccctta agggttcgat ccctaccggt tagtaatgag tttgatatct 3361 cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt 3421 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc 3481 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga 3541 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc 3601 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct 3661 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg 3721 gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct 3781 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc 3841 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg 3901 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggaaa 3961 cgggggaggc taactgaaac acggaaggag acaataccgg aaggaacccg cgctatgacg 4021 gcaataaaaa gacagaataa aacgcacggg tgttgggtcg tttgttcata aacgcggggt 4081 tcggtcccag ggctggcact ctgtcgatac cccaccgaga ccccattggg gccaatacgc 4141 ccgcgtttct tccttttccc caccccaccc cccaagttcg ggtgaaggcc cagggctcgc 4201 agccaacgtc ggggcggcag gccctgccat agcagatctg cgcagctggg gctctagggg 4261 gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag 4321 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt 4381 tctcgccacg ttcgccggct ttccccgtca agctctaaat cggggcatcc ctttagggtt 4441 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg 4501 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt 4561 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt 4621 tgatttataa gggattttgg ggatttcggc ctattggtta aaaaatgagc tgatttaaca 4681 aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca 4741 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt 4801 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca 4861 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc 4921 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct 4981 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa 5041 aagctcccgg gagcttgtat atccattttc ggatctgatc aagagacagg atgaggatcg 5101 tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg 5161 ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg 5221 ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat 5281 gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca 5341 gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg 5401 gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat 5461 gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa 5521 catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg 5581 gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg 5641 cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg 5701 gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat 5761 caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac 5821 cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc 5881 cttcttgacg agttcttctg agcgggactc tggggttcgc gaaatgaccg accaagcgac 5941 gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt 6001 cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga 6061 gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag 6121 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa 6181 actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta 6241 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat 6301 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt 6361 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta 6421 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc 6481 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa 6541 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa 6601 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct 6661 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac 6721 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc 6781 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc 6841 tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg 6901 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga 6961 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag 7021 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta 7081 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag 7141 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg 7201 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac 7261 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc 7321 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag 7381 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc 7441 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac 7501 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc 7561 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg 7621 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag 7681 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc 7741 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac 7801 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag 7861 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac 7921 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg 7981 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc 8041 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact 8101 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg 8161 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa 8221 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt 8281 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg 8341 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga 8401 cgtcgacgga tcgggagatc tcccgatccc ctatggtcga ctctcagtac aatctgctct 8461 gatgccgcat agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctgagtag 8521 tgcgcgagca aaatttaagc tacaacaagg caaggcttga ccgacaattg catgaagaat 8581 ctgcttagg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.