pLV3-CMV-TRIM21-3×HA-mCherry-H

pLV3-CMV-TRIM21-3×HA-mCherry-Hyg plasmid

PVT103647

pLV3-CMV-TRIM21-3×HA-mCherry-Hyg plasmid Information

Plasmid name: pLV3-CMV-TRIM21(human)-3×HA-mCherry-Hyg
Alias: NM_003141.4;RNF81; Ro/SSA; RO52; SSA; SSA1;
Gene lenght: 1425
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Hyg
Fluorescent labeling: Red
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-TRIM21-3×HA-mCherry-Hyg plasmid Description

pLV3-CMV-TRIM21-3×HA-mCherry-Hyg It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-TRIM21-3×HA-mCherry-Hyg plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt 1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca 1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc 2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga 2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat 2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcgtttag 2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct 2281 agagctagcC TTAAGCTTGG TACCGAGCTC GAATTCCTCG AGGCCACCat ggcttcagca 2341 gcacgcttga caatgatgtg ggaggaggtc acatgcccta tctgcctgga ccccttcgtg 2401 gagcctgtga gcatcgagtg tggccacagc ttctgccagg aatgcatctc tcaggttggg 2461 aaaggtgggg gcagcgtctg tcctgtgtgc cggcagcgct ttctgctcaa gaatctccgg 2521 cccaatcgac agctagccaa catggtgaac aaccttaaag aaatcagcca ggaggccaga 2581 gagggcacac agggggaacg gtgtgcagtg catggagaga gacttcacct gttctgtgag 2641 aaagatggga aggccctttg ctgggtatgt gcccagtctc ggaaacaccg tgaccacgcc 2701 atggtccctc ttgaggaggc tgcacaggag taccaggaga agctccaggt ggcattaggg 2761 gaactgagaa gaaagcagga gttggctgag aagttggaag tggaaattgc aataaagaga 2821 gcagactgga agaaaacagt ggaaacacag aaatctagga ttcacgcaga gtttgtgcag 2881 caaaaaaact tcctggttga agaagaacag aggcagctgc aggagctgga gaaggatgag 2941 agggagcagc tgagaatcct gggggagaaa gaggccaagc tggcccagca gagccaggcc 3001 ctacaggagc tcatctcaga gctagatcga aggtgccaca gctcagcact ggaactgctg 3061 caggaggtga taattgtcct ggaaaggagt gagtcctgga acctgaagga cctggatatt 3121 acctctccag aactcaggag tgtgtgccat gtgccagggc tgaagaagat gctgaggaca 3181 tgtgcagtcc acatcactct ggatccagac acagccaatc cgtggctgat actttcagaa 3241 gatcggagac aagtgaggct tggagacacc cagcagagca tacctggaaa tgaagagaga 3301 tttgatagtt atcctatggt cctgggtgcc cagcactttc actctggaaa acattactgg 3361 gaggtagatg tgacaggaaa ggaggcctgg gacctgggtg tctgcagaga ctctgtgcgc 3421 aggaaggggc actttttgct tagttccaag agtggcttct ggacaatttg gttgtggaac 3481 aaacaaaaat atgaggctgg cacctacccc cagactcccc tccaccttca ggtgcctcca 3541 tgccaagttg ggattttcct ggactatgag gctggcatgg tctccttcta caacatcact 3601 gaccatggct ccctcatcta ctccttctct gaatgtgcct ttacaggacc tctgcggccc 3661 ttcttcagtc ctggtttcaa tgatggagga aaaaacacag cccctctaac cctctgtcca 3721 ctgaatattg gatcacaagg atccactgac tatTACCCAT ACGACGTACC TGATTATGCA 3781 ACTTATCCTT ATGATGTACC TGATTATGCT AGCTATCCTT ATGATGTCCC AGACTACGCA 3841 TAGgcggccg cGaaggatct gcgatcgctc cggtgcccgt cagtgggcag agcgcacatc 3901 gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaacgggtg cctagagaag 3961 gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg 4021 tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt 4081 tgccgccaga acacagctga agcttcgagg ggctcgcatc tctccttcac gcgcccgccg 4141 ccctacctga ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc ccgcctgtgg 4201 tgcctcctga actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga gaccgggcct 4261 ttgtccggcg ctcccttgga gcctacctag actcagccgg ctctccacgc tttgcctgac 4321 cctgcttgct caactctacg tctttgtttc gttttctgtt ctgcgccgtt acagatccaa 4381 gctgtgaccg gcgcctacgc tagacgccac cATGGTGAGC AAGGGCGAGG AGGATAACAT 4441 GGCCATCATC AAGGAGTTCA TGCGCTTCAA GGTGCACATG GAGGGCTCCG TGAACGGCCA 4501 CGAGTTCGAG ATCGAGGGCG AGGGCGAGGG CCGCCCCTAC GAGGGCACCC AGACCGCCAA 4561 GCTGAAGGTG ACCAAGGGTG GCCCCCTGCC CTTCGCCTGG GACATCCTGT CCCCTCAGTT 4621 CATGTACGGC TCCAAGGCCT ACGTGAAGCA CCCCGCCGAC ATCCCCGACT ACTTGAAGCT 4681 GTCCTTCCCC GAGGGCTTCA AGTGGGAGCG CGTGATGAAC TTCGAGGACG GCGGCGTGGT 4741 GACCGTGACC CAGGACTCCT CCCTGCAGGA CGGCGAGTTC ATCTACAAGG TGAAGCTGCG 4801 CGGCACCAAC TTCCCCTCCG ACGGCCCCGT AATGCAGAAG AAGACCATGG GCTGGGAGGC 4861 CTCCTCCGAG CGGATGTACC CCGAGGACGG CGCCCTGAAG GGCGAGATCA AGCAGAGGCT 4921 GAAGCTGAAG GACGGCGGCC ACTACGACGC TGAGGTCAAG ACCACCTACA AGGCCAAGAA 4981 GCCCGTGCAG CTGCCCGGCG CCTACAACGT CAACATCAAG TTGGACATCA CCTCCCACAA 5041 CGAGGACTAC ACCATCGTGG AACAGTACGA ACGCGCCGAG GGCCGCCACT CCACCGGCGG 5101 CATGGACGAG CTGTACAAGG TCGAGGGAAG CGGAGCTACT AACTTCAGCC TGCTGAAGCA 5161 GGCTGGAGAC GTGGAGGAGA ACCCTGGACC TGTCGACatg aaaaagcctg aactcaccgc 5221 gacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc gtctccgacc tgatgcagct 5281 ctcggagggc gaagaatctc gtgctttcag cttcgatgta ggagggcgtg gatatgtcct 5341 gcgggtaaat agctgcgccg atggtttcta caaagatcgt tatgtttatc ggcactttgc 5401 atcggccgcg ctcccgattc cggaagtgct tgacattggg gaattcagcg agagcctgac 5461 ctattgcatc tcccgccgtg cacagggtgt cacgttgcaa gacctgcctg aaaccgaact 5521 gcccgctgtt ctgcagccgg tcgcggaggc catggatgcg atcgctgcgg ccgatcttag 5581 ccagacgagc gggttcggcc cattcggacc gcaaggaatc ggtcaataca ctacatggcg 5641 tgatttcata tgcgcgattg ctgatcccca tgtgtatcac tggcaaactg tgatggacga 5701 caccgtcagt gcgtccgtcg cgcaggctct cgatgagctg atgctttggg ccgaggactg 5761 ccccgaagtc cggcacctcg tgcacgcgga tttcggctcc aacaatgtcc tgacggacaa 5821 tggccgcata acagcggtca ttgactggag cgaggcgatg ttcggggatt cccaatacga 5881 ggtcgccaac atcttcttct ggaggccgtg gttggcttgt atggagcagc agacgcgcta 5941 cttcgagcgg aggcatccgg agcttgcagg atcgccgcgg ctccgggcgt atatgctccg 6001 cattggtctt gaccaactct atcagagctt ggttgacggc aatttcgatg atgcagcttg 6061 ggcgcagggt cgatgcgacg caatcgtccg atccggagcc gggactgtcg ggcgtacaca 6121 aatcgcccgc agaagcgcgg ccgtctggac cgatggctgt gtagaagtac tcgccgatag 6181 tggaaaccga cgccccagca ctcgtccgag ggcaaaggaa tagTCTAGAa atcaacctct 6241 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct 6301 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat 6361 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt 6421 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat 6481 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc 6541 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga 6601 caattccgtg gtgttgtcgg ggaaATCATC GTCCTTTCCT tggctgctcg cctgtgttgc 6661 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga 6721 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtctcc gccttcgccc 6781 tcagacgagt cggatctccc tttgggccgc ctccccgcct ggtaccttta agaccaatga 6841 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc 6901 taattcactc ccaacgaaga taagatctgc tttttgcttg tactgggtct ctctggttag 6961 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat 7021 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact 7081 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc 7141 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact 7201 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata 7261 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc 7321 atgtctggct ctagctatcc cgcccctaac tccgccATCC CGCCCTAACT CCGCcagttc 7381 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc 7441 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc tagacttttg 7501 cagagacggc ccaaattcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc 7561 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta 7621 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa 7681 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat 7741 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg 7801 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc 7861 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt 7921 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag 7981 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc 8041 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc 8101 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt 8161 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt 8221 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc 8281 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa 8341 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa 8401 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg 8461 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga 8521 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg 8581 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg 8641 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt 8701 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact 8761 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat 8821 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg 8881 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg 8941 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat 9001 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc 9061 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt 9121 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc 9181 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga 9241 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc 9301 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa 9361 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta 9421 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg 9481 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg 9541 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat 9601 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt 9661 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa 9721 aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct 9781 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag 9841 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc 9901 ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg 9961 cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga 10021 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc 10081 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc 10141 cagtgccaag ctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.