pCMV-Prrc2b-3×FLAG-Neo plasmid

pCMV-Prrc2b-3×FLAG-Neo plasmid

PVT103544

pCMV-Prrc2b-3×FLAG-Neo plasmid Information

Plasmid name: pCMV-Prrc2b(mouse)-3×FLAG-Neo
Alias: NM_001159634.1;5830434P21Rik; Bat2l; Bat2l1; D430039P21;mKIAA0515
Gene lenght: 6690
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-Prrc2b-3×FLAG-Neo plasmid Description

pCMV-Prrc2b-3×FLAG-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-Prrc2b-3×FLAG-Neo plasmid Sequence

1 GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA 61 TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG 121 TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC 181 CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA 241 CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT 301 ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC 361 CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT 421 ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA 481 CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT 541 CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG 601 CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTCTGGC TAACTAGAGA ACCCACTGCT 661 TACTGGCTTA TCGAAATTAA TACGACTCAC TATAGGGAGA CCCAAGCTGG CTAGCGTTTA 721 AACTTAAGCT TGGTACCGAG CTCGAATTCg ccaccatgtc cgatcgtttg gggcaaataa 781 cccagggcaa ggatgggaaa agcaagtact cgactctcag cctgtttgac aagtataaag 841 ggaggtcagt aggcgctgtc aggtcctcag ttattcctag acatggctta cagagtctcg 901 ggaaagttgc cacagcccgg cgtatgccac cgcctgcaaa cctgccaagc ctgaagtctg 961 aaaacaaagg aaacgacccc aacatcgtga tagttcccaa ggacgggaca ggatgggcca 1021 acaagcagga ccagcaagac ccaaagagtt ccagtgtgac ggcctctcag ccgccggagt 1081 cgcagccgca gccgggtttg cagaaatctg tctccaattt gcagaaaccg acacagtcta 1141 tcagtcagga gaacacaaat tcagtgccag gtggaccaaa gtcatgggca cagctgagtg 1201 gaaagccagt cggacacgaa ggtggtttaa ggggctcaag ccgactgtta tccttctctc 1261 ccgaggaatt tccgacgctg aaagcagctg gagggcagga caaggctggc aaagaaaagg 1321 gcgccttaga tctgtcgtat gggccaggac caagcctccg ccctcagaat gtgacaagct 1381 ggagggaggg cggtgggcgg aacatcattt ctgccgcctc tctgagcgcc tccccaacag 1441 agctgggcag caggaacgcc agtggggcag acggagcccc ttccttggca tgcaccagcg 1501 attctaagga gccctctctc cgcccagctc agcccagccg cagaggggcc tcccaattca 1561 tggggcacgg ttaccagcca cccacgtacc atgacatgct cccagcgttt atgtgttcac 1621 cacagtcatc agagaaccag actacggtgg aacgaagctc ttttcccctt cctcagctcc 1681 gcctggaacc ccgtgttcct ttcagacagt tccagatgaa cgaccaagat gggaaagaaa 1741 ggccaggcgt ggcacggcca gtgcgcccac tgaggcagct ggtggagagg gcaccaaggc 1801 ccaccatcat caacgcggaa aacctgaagg gcttggatga tctggacact gatgcggatg 1861 atgggtgggc aggcctccac gaggaagtgg actattccga gaaactgaag ttcagtgatg 1921 atgaagatga ggaggatgtt gtgaaggatg gccggtcaaa gtggaacaac tgggatccaa 1981 ggcggcagcg ggcgctgtca ttgagctctg cagacagcac tgacgccaag cgcactcagg 2041 aggaagggaa ggactggagt ggaacagcag gtggatcccg tgtcatccga aaggtgccag 2101 agcctcagcc tccttccagg aagcttcaca gttgggcctc tgggcctgac taccagaaac 2161 ccacaatggg cagcatgttc cgccagcact ccgctgagga caaggaggac aagccacccc 2221 cacggcagaa gttcatccag tcagagatgt ccgaggccgt ggaacgagcc cgaaagcgca 2281 gggaagaaga agagcgccgg gcccgagagg agaggctggc tgcctgtgct gccaaactca 2341 agcagctaga ccagaagtgc aggcaggctc agaaggccaa tgagaccccg aagccagtgg 2401 aaaaggaagt cccccgatcc ccgggcattg agaaggtctc cccgccagag aatgggcctg 2461 ttgtccgcaa aggctcccca gagttccctg tccaagaagc acccaccatg ttcttggaag 2521 agacacctgc aacatcccca acagtcgctc agagcaatag cagcagcagc agtagcagca 2581 gtagcagcat cgaggaggaa gtccgggagt ccgggtcccc tgcacaggag ttcagcaagt 2641 accagaagtc ccttcctccc cgtttccagc gccagcagca gcagcaacag cagcaacagc 2701 agcagcagca gcagcaggag cagctgtaca agatgcagca ctggcagccg gtctaccccc 2761 caccttctca cccccagcgc accttctacc cacaccaccc ccagatgctg ggctttgacc 2821 cacggtggat gatgatgcct tcctacatgg acccacgcat caccccgaca cggacgcccg 2881 tggacttcta cccctcagcc ctgcatcctt caggactgat gaagcccatg atgccccaag 2941 agtccctcag tgggactggc tgtcgctctg aagatcaaaa ctgtgtgccc tcgctccaag 3001 aacggaaagt gaccgccctc gacccagctc ctgtgtggag cccagagggc tacatggcac 3061 tgcagaacaa aggctactca ctgccccacc cgaagtctgc tgacactctg gccatgggca 3121 tgcatgtcag gaatgaaagg tcttactgtg cctcccccgg aaggccaggg ggcataagtg 3181 cccagcgcga tctctttgag gagagagggg aggagttctt gagtgctttt gacaagaagg 3241 cccaagcaga ctttgacagc tgtatctctt ctcaaagaat aggccaggag cttttatttc 3301 caccccagga aaatgttcag gaggtgggtg ctcctggggg ttgtacccca aacctcaggt 3361 gttccccatt ggagcctgac tttgtcccag ctgagaaaaa gccagagtat ggcagctggg 3421 atgttggcca ccagccaaag gccgccgaca cagccaacgg tgtcgagctg gaggctcccc 3481 gacaggagcc ttcctttcat gtttcctctt gggagaagga agggagcccc aagaaacagc 3541 caaacccgga gcctgagtgg acacctgagc cccggagctc cagcggccag caccaggagc 3601 agccgggcag gacccggagg tcaggaccca tcaagaaacc agttcttaag gccctgaagg 3661 tagaagagaa ggagaaggcc ctggagagag gcaggcaggg gcttagagag gagagctcac 3721 agcgggcccc agagaaagaa cccgtgggca gggccgagga ggacgaagag aacaaccctg 3781 ccctagccaa tgcctcctcc gccctggagg acaaggctgc ctcccgggct ggttttgccc 3841 acgaggctag caagctggat gaagatgaaa aggcagataa gacctgggag agcaggccct 3901 caagggaggc cagtgacatt cccccaacca agagaaacaa ctggatcttc attgatgagg 3961 aacaggcctt tgggggacgg ggccaggccc gtagccgtgg ccgcggcttc agagagttca 4021 ccttccgggg cggccggcct gcaggcagca gcacaagtgg tctttgtggc acgggtgtcc 4081 ttgggtctcg tggcatgtac agtagtggcc agcgcagtaa ccgaggcagg ggcctgcgtg 4141 acttcccccc accagaagac tgccccagag ccaagccaag gcgtcgcatt gccagcgaga 4201 ctcacagcga gggctctgag tatgaagagc tgcccaagag gcgccggcag cggggctctg 4261 agcatagcca cgagggcatg ctcacggaga gggacgaggg tgccctgaag gactcatggc 4321 gctccaacag aacttacacc gaggaccagg gcggtataga cacccgcagc cggggatccc 4381 ggacatgcgg gagagccctc ccaccccgcc tgagcaactg cagctatggc cgcagaacct 4441 ttgttgccaa agaacctccc cactggcaga gcaggagccc aggcagctcc tggcaggaat 4501 atggctcctc tgacccgtgt ggcccacggc gaggcaccga cagagactac ataccagact 4561 cctaccgaca atctgacaca tttggaagca ggctttttga ggacagccgc acggaggaca 4621 agaggtcctt tttccaagat gaccatgggg cagattctga aaatgcagaa aacaggccct 4681 tccgtaggag gcggccccca cgccaagaca agccccctcg cttccggcgc ctccgtcaag 4741 agcgggagtc cctgggcctg tggggacctg aggaggagtc ccatctgctg gcaagccagt 4801 ggccaggccg gtccaaactc tgtcctgggg acaagagtgg tcctggaggt cacagatccc 4861 cagagctttc ctaccagaat tcctctgatc atgccaacga ggagtgggag accgcctccg 4921 agagtagtga cttcagcgag cgtagggagc ggcgagaagg cctcgtggct gaacccgaag 4981 cgcaagggga tggtggcctc tctgggtcca gtttgggtga aaagaaggag ctggccaaga 5041 ggagcttctc cagccagagg cccctggccg acagacagag ccgcaagctg gagccgggag 5101 ggtttgggga gaagcctgtt aggccaggtg ggggtgagcc ttccccccgc tgcgagagcc 5161 agcagagcgg gacgcctctg aaagtcaaaa ggtctccaga tgaggccttg cctggaggcc 5221 ttggcagcca ttcaccctat gccttggaga gaaccactca cgccagctct gacggtcctg 5281 aaacccccag taagaaatca gagagagagg tttctttgcc tacccagagg gctagtgagc 5341 aggaagaggc ccggaagcag tttgacctgg gctatggaaa tgctctcatt gacaactgcg 5401 cctccagccc tggggaagag aatgaagcaa gctctgtggt gggtgaaggc ttcattgaag 5461 tcctgaccaa gaagcagcgt cgcctgctgg aggaggagag aagaaaaaaa gaacaggctg 5521 ctcaggtgcc tgtcaaaggt cggggccttt catcccggat tcctcctcgg tttgctaaaa 5581 agcagaacgg cctgtgtctg gagcaagatg tgactgtgcc tggcagcagc ctgggcactg 5641 agatctggga gaacagcagc caagccctcc ctgtgcaggg cgccgccagt gactcatgga 5701 ggacagctgt cactgccttc agcagtactg agcctggcac ctcagagggg tttaagagca 5761 gccagggaga tagtggcgtt gacttgagtg ctgagtctcg ggagtcgtct gcgacctcct 5821 cacagcgcag ttccccatat ggcactctca agcctgagga aattagcggg cctggcctgg 5881 cggaatctaa ggccgacagc cacaaggacc aagctcagaa gcaggcggag cacaaggact 5941 cagaacaagg ctctgcacag agcaaggagc acagaccagg acccatcggc aacgagcggt 6001 ctctgaaaaa cagaaagggc tctgaggggg ccgagcggct gccaggggcc gttgtcccgc 6061 ctgttaatgg ggtggagatt cacgtggact ccgtgctgcc ggtgccgccc attgagttcg 6121 gagtcagtcc aaaagactct gatttcagcc tgccacccgg gtctgtttct ggtcctgtag 6181 ggaatccagt ggccaagctt caggacgtct tggctagtaa tgcaggactg acgcagagta 6241 ttcccatcct gcggcgggat caccacatgc agagagccat cggcctctct cccatgtctt 6301 tccccaccgc agacctcaca ctaaagatgg agtctgcacg caaggcttgg gaaaactccc 6361 ccagtttgcc agagcagagc tctccaggag gtgcaggctc gggcatccag cctccatcct 6421 ctgtgggcgc ctccaatggg gtcaactaca gctcctttgg tggagtgtcg atgccgccca 6481 tgcccgtggc ttctgtagcg ccttctgctt cgataccagg cagccacctc ccacctctgt 6541 acctggatgg ccacgtgttt gcaagtcagc cccgactggt tcctcaaacg atacctcagc 6601 agcagagcta ccagcaggcc gccactgccc agcagatccc gatatccctt cacacgtctc 6661 tgcaggccca ggcccagctt gggctgagag gtgggctccc cgtctcccag tcccaggaga 6721 tcttcagctc cctgcagccc ttcaggtctc aggtgtacat gcaccccagc ctgtcaccac 6781 ccagcacgat gatcctctca gggggtacag ccttgaagcc cccatactct gcatttcctg 6841 gcatacagcc cttggagatg gtgaagcccc agtctggctc accctaccag cccatgagtg 6901 gaaaccaggc cctggtctac gagggccagc ttggccaggc tgccgggctg ggcacctccc 6961 agatgctgga ttcccagctc ccacagctca ctatgccact gcctcggtat ggctccgggc 7021 agcagccact gatcctgcca cagtccatcc agctgcctcc aggacagagt ctctctgttg 7081 gagccccccg gagggttcct ccacctggct cccaacctcc cgtcctgaac accagcagag 7141 agtccgctcc gatggagttg aaaggcttcc actttgccga cagtaaacag aatgttccta 7201 ctggagggtc cgcaccatcc ccgcaggcat acaggcctag ctctgctagc cccagtggga 7261 agccctctgg atcagcagtt aacatgggct ctgtgcaggg acactacgtt caacaggcaa 7321 agcgagtgga tgaaaaaccc ggcctgggaa ccgtgaagct gcaggaggcc tcatcagcca 7381 cctcccagat gaagcgaacc ggagcaatca agcctcgggc agtcaaggtg gagggaagca 7441 aagccGGATC CGACTACAAA GACCATGACG GTGATTATAA AGATCATGAC ATCGACTACA 7501 AGGATGACGA TGACAAGTAG TGAGGGCCCG ATATCTCGAC AATCAACCTC TGGATTACAA 7561 AATTTGTGAA AGATTGACTG GTATTCTTAA CTATGTTGCT CCTTTTACGC TATGTGGATA 7621 CGCTGCTTTA ATGCCTTTGT ATCATGCTAT TGCTTCCCGT ATGGCTTTCA TTTTCTCCTC 7681 CTTGTATAAA TCCTGGTTGC TGTCTCTTTA TGAGGAGTTG TGGCCCGTTG TCAGGCAACG 7741 TGGCGTGGTG TGCACTGTGT TTGCTGACGC AACCCCCACT GGTTGGGGCA TTGCCACCAC 7801 CTGTCAGCTC CTTTCCGGGA CTTTCGCTTT CCCCCTCCCT ATTGCCACGG CGGAACTCAT 7861 CGCCGCCTGC CTTGCCCGCT GCTGGACAGG GGCTCGGCTG TTGGGCACTG ACAATTCCGT 7921 GGTGTTGTCG GGGAAGCTGA CGTCCTTTCC ATGGCTGCTC GCCTGTGTTG CCACCTGGAT 7981 TCTGCGCGGG ACGTCCTTCT GCTACGTCCC TTCGGCCCTC AATCCAGCGG ACCTTCCTTC 8041 CCGCGGCCTG CTGCCGGCTC TGCGGCCTCT TCCGCGTCTT CGCCTTCGCC CTCAGACGAG 8101 TCGGATCTCC CTTTGGGCCG CCTCCCCGCC TGGAAACGGG GGAGGCTAAC TGAAACACGG 8161 AAGGAGACAA TACCGGAAGG AACCCGCGCT ATGACGGCAA TAAAAAGACA GAATAAAACG 8221 CACGGGTGTT GGGTCGTTTG TTCATAAACG CGGGGTTCGG TCCCAGGGCT GGCACTCTGT 8281 CGATACCCCA CCGAGACCCC ATTGGGGCCA ATACGCCCGC GTTTCTTCCT TTTCCCCACC 8341 CCACCCCCCA AGTTCGGGTG AAGGCCCAGG GCTCGCAGCC AACGTCGGGG CGGCAGGCCC 8401 TGCCATAGCA GATCTGCGCA GCTGGGGCTC TAGGGGGTAT CCCCACGCGC CCTGTAGCGG 8461 CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC 8521 CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC 8581 CCGTCAAGCT CTAAATCGGG GCATCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT 8641 CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC 8701 GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC 8761 TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGGGGAT 8821 TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTAATTCTG 8881 TGGAATGTGT GTCAGTTAGG GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG CAGAAGTATG 8941 CAAAGCATGC ATCTCAATTA GTCAGCAACC AGGTGTGGAA AGTCCCCAGG CTCCCCAGCA 9001 GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCATAGTCCC GCCCCTAACT 9061 CCGCCCATCC CGCCCCTAAC TCCGCCCAGT TCCGCCCATT CTCCGCCCCA TGGCTGACTA 9121 ATTTTTTTTA TTTATGCAGA GGCCGAGGCC GCCTCTGCCT CTGAGCTATT CCAGAAGTAG 9181 TGAGGAGGCT TTTTTGGAGG CCTAGGCTTT TGCAAAAAGC TCCCGGGAGC TTGTATATCC 9241 ATTTTCGGAT CTGATCAAGA GACAGGATGA GGATCGTTTC GCATGATTGA ACAAGATGGA 9301 TTGCACGCAG GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT TCGGCTATGA CTGGGCACAA 9361 CAGACAATCG GCTGCTCTGA TGCCGCCGTG TTCCGGCTGT CAGCGCAGGG GCGCCCGGTT 9421 CTTTTTGTCA AGACCGACCT GTCCGGTGCC CTGAATGAAC TGCAGGACGA GGCAGCGCGG 9481 CTATCGTGGC TGGCCACGAC GGGCGTTCCT TGCGCAGCTG TGCTCGACGT TGTCACTGAA 9541 GCGGGAAGGG ACTGGCTGCT ATTGGGCGAA GTGCCGGGGC AGGATCTCCT GTCATCTCAC 9601 CTTGCTCCTG CCGAGAAAGT ATCCATCATG GCTGATGCAA TGCGGCGGCT GCATACGCTT 9661 GATCCGGCTA CCTGCCCATT CGACCACCAA GCGAAACATC GCATCGAGCG AGCACGTACT 9721 CGGATGGAAG CCGGTCTTGT CGATCAGGAT GATCTGGACG AAGAGCATCA GGGGCTCGCG 9781 CCAGCCGAAC TGTTCGCCAG GCTCAAGGCG CGCATGCCCG ACGGCGAGGA TCTCGTCGTG 9841 ACCCATGGCG ATGCCTGCTT GCCGAATATC ATGGTGGAAA ATGGCCGCTT TTCTGGATTC 9901 ATCGACTGTG GCCGGCTGGG TGTGGCGGAC CGCTATCAGG ACATAGCGTT GGCTACCCGT 9961 GATATTGCTG AAGAGCTTGG CGGCGAATGG GCTGACCGCT TCCTCGTGCT TTACGGTATC 10021 GCCGCTCCCG ATTCGCAGCG CATCGCCTTC TATCGCCTTC TTGACGAGTT CTTCTGAGCG 10081 GGACTCTGGG GTTCGCGAAA TGACCGACCA AGCGACGCCC AACCTGCCAT CACGAGATTT 10141 CGATTCCACC GCCGCCTTCT ATGAAAGGTT GGGCTTCGGA ATCGTTTTCC GGGACGCCGG 10201 CTGGATGATC CTCCAGCGCG GGGATCTCAT GCTGGAGTTC TTCGCCCACC CCAACTTGTT 10261 TATTGCAGCT TATAATGGTT ACAAATAAAG CAATAGCATC ACAAATTTCA CAAATAAAGC 10321 ATTTTTTTCA CTGCATTCTA GTTGTGGTTT GTCCAAACTC ATCAATGTAT CTTATCATGT 10381 CTGTATACCG TCGACCTCTA GCTAGAGCTT GGCGTAATCA TGGTCATAGC TGTTTCCTGT 10441 GTGAAATTGT TATCCGCTCA CAATTCCACA CAACATACGA GCCGGAAGCA TAAAGTGTAA 10501 AGCCTGGGGT GCCTAATGAG TGAGCTAACT CACATTAATT GCGTTGCGCT CACTGCCCGC 10561 TTTCCAGTCG GGAAACCTGT CGTGCCAGCT GCATTAATGA ATCGGCCAAC GCGCGGGGAG 10621 AGGCGGTTTG CGTATTGGGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT 10681 CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 10741 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 10801 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 10861 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 10921 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 10981 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAA TGCTCACGCT GTAGGTATCT 11041 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 11101 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 11161 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 11221 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGGACAG TATTTGGTAT 11281 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 11341 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 11401 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 11461 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 11521 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA 11581 CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC 11641 CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG 11701 CCCCAGTGCT GCAATGATAC CGCGAGACCC ACGCTCACCG GCTCCAGATT TATCAGCAAT 11761 AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT 11821 CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG 11881 CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC 11941 ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA 12001 AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC 12061 ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT 12121 TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG 12181 TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT 12241 GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG 12301 ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC 12361 CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC 12421 GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA 12481 GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG 12541 GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGTC GACGGATCGG GAGATCTCCC 12601 GATCCCCTAT GGTCGACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT 12661 CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG CGAGCAAAAT TTAAGCTACA 12721 ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC TTAGG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.