pLV3-CMV-CLDN5-EGFP-EF1a-Puro

pLV3-CMV-CLDN5-EGFP-EF1a-Puro plasmid

PVT102797

pLV3-CMV-CLDN5-EGFP-EF1a-Puro plasmid Information

Plasmid name: pLV3-CMV-CLDN5(human)-EGFP-EF1a-Puro
Alias: NM_001130861.1;AWAL; BEC1; CPETRL1; TMDVCF; TMVCF
Gene lenght: 909
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-CLDN5-EGFP-EF1a-Puro plasmid Description

pLV3-CMV-CLDN5-EGFP-EF1a-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-CLDN5-EGFP-EF1a-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt 1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc 1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga 1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac 1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga 2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg 2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg 2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac 2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagagc 2281 tagcgaattc gccaccatga cccgcgcacg gattggctgc ttcgggccgg ggggccgggc 2341 ccgggggaca gaatccgccc ccgaaccttc aaagagggta ccccccggca ggagctggca 2401 gacccaggag gtgcgacaga cccgcggggc aaacggactg gggccaagag ccgggagcgc 2461 gggcgcaaag gcaccagggc ccgcccaggg cgccgcgcag cacggccttg ggggttctgc 2521 gggccttcgg gtgcgcgtct cgcctctagc catggggtcc gcagcgttgg agatcctggg 2581 cctggtgctg tgcctggtgg gctggggggg tctgatcctg gcgtgcgggc tgcccatgtg 2641 gcaggtgacc gccttcctgg accacaacat cgtgacggcg cagaccacct ggaaggggct 2701 gtggatgtcg tgcgtggtgc agagcaccgg gcacatgcag tgcaaagtgt acgactcggt 2761 gctggctctg agcaccgagg tgcaggcggc gcgggcgctc accgtgagcg ccgtgctgct 2821 ggcgttcgtt gcgctcttcg tgaccctggc gggcgcgcag tgcaccacct gcgtggcccc 2881 gggcccggcc aaggcgcgtg tggccctcac gggaggcgtg ctctacctgt tttgcgggct 2941 gctggcgctc gtgccactct gctggttcgc caacattgtc gtccgcgagt tttacgaccc 3001 gtctgtgccc gtgtcgcaga agtacgagct gggcgcagcg ctgtacatcg gctgggcggc 3061 caccgcgctg ctcatggtag gcggctgcct cttgtgctgc ggcgcctggg tctgcaccgg 3121 ccgtcccgac ctcagcttcc ccgtgaagta ctcagcgccg cggcggccca cggccaccgg 3181 cgactacgac aagaagaact acgtcggatc cGGAGGAGGG GGAAGCatgg tgagcaaggg 3241 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg 3301 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct 3361 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct 3421 gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt 3481 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg 3541 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga 3601 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa 3661 ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa 3721 cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca 3781 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca 3841 gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt 3901 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaagcgg ccgcgaagga 3961 tctgcgatcg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga 4021 agttgggggg aggggtcggc aattgaacgg gtgcctagag aaggtggcgc ggggtaaact 4081 gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata 4141 taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc 4201 tgaagcttcg aggggctcgc atctctcctt cacgcgcccg ccgccctacc tgaggccgcc 4261 atccacgccg gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt 4321 ccgccgtcta ggtaagttta aagctcaggt cgagaccggg cctttgtccg gcgctccctt 4381 ggagcctacc tagactcagc cggctctcca cgctttgcct gaccctgctt gctcaactct 4441 acgtctttgt ttcgttttct gttctgcgcc gttacagatc caagctgtga ccggcgccta 4501 cgctagatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtcccccgg 4561 gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgac 4621 ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg 4681 ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg 4741 ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg 4801 agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc 4861 aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt 4921 ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc 4981 ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc 5041 accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt 5101 gcctgagtcg acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt 5161 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct 5221 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt 5281 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac 5341 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct 5401 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca 5461 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt 5521 ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc 5581 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct 5641 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg 5701 cctggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa 5761 agaaaagggg ggactggaag ggctaattca ctcccaacga aaataagatc tgctttttgc 5821 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg 5881 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg 5941 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat 6001 ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg 6061 aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa 6121 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc 6181 caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc 6241 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag 6301 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctagac 6361 ttttgcagag acggcccaaa ttcgtaatca tggtcatagc tgtttcctgt gtgaaattgt 6421 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt 6481 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg 6541 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg 6601 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg 6661 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat 6721 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc 6781 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc 6841 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga 6901 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt 6961 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg 7021 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc 7081 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg 7141 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc 7201 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg 7261 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc 7321 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct 7381 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt 7441 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa 7501 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa 7561 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc 7621 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct 7681 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca 7741 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt 7801 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt 7861 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc 7921 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc 7981 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt 8041 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact 8101 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc 8161 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt 8221 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg 8281 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct 8341 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa 8401 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt 8461 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc 8521 acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc 8581 tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa 8641 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg 8701 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac 8761 tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac 8821 agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc cattcaggct gcgcaactgt 8881 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt 8941 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg 9001 acggccagtg ccaagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.