pLV3-CMV-KLHDC2-3×FLAG-P2A-EGF

pLV3-CMV-KLHDC2-3×FLAG-P2A-EGFP plasmid

PVT102295

pLV3-CMV-KLHDC2-3×FLAG-P2A-EGFP plasmid Information

Plasmid name: pLV3-CMV-KLHDC2(human)-3×FLAG-P2A-EGFP
Alias: NM_014315.3;HCLP-1; HCLP1; LCP
Gene lenght: 1218
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-KLHDC2-3×FLAG-P2A-EGFP plasmid Description

pLV3-CMV-KLHDC2-3×FLAG-P2A-EGFP It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-KLHDC2-3×FLAG-P2A-EGFP plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt 1921 atcgatacta gtcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga 1981 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt 2041 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt 2101 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca 2161 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt 2221 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt 2281 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca 2341 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg 2401 cggtaggcgt gtacggtggg aggtctatat aagcagagct ggtttagtga accgtcagat 2461 ccgctagcgc caccatggct gatggcaacg aggatctgcg ggctgacgac ttgcctgggc 2521 cagccttcga gagctatgag tccatggagc ttgcctgccc cgctgagcgc agcggccacg 2581 tagccgtcag cgacgggcgc cacatgttcg tctggggcgg ctacaagagt aatcaagtca 2641 gaggattata tgacttttat ctgcctagag aagaactatg gatctacaac atggagactg 2701 gaagatggaa aaaaatcaac actgaaggtg atgttcctcc ttctatgtca ggaagctgtg 2761 ctgtgtgtgt agacagggtg ctgtacttgt ttggaggaca ccattcaaga ggcaatacca 2821 ataagttcta catgctggat tcaaggtcta cagacagagt gttacagtgg gaaagaattg 2881 attgccaagg aattcctcca tcatcaaagg acaaacttgg tgtctgggta tataaaaaca 2941 agttaatatt ttttggaggg tatggatatt tgcctgaaga taaagtattg ggaacttttg 3001 aattcgatga aacatctttt tggaattcaa gtcatccaag aggatggaat gatcatgtac 3061 atattttaga tactgaaaca tttacctgga gccagcctat aactactggt aaagcacctt 3121 cacctcgtgc tgcccatgcc tgtgcaactg tcggaaatag aggcttcgtg tttggaggca 3181 gatatcgaga tgctagaatg aatgatcttc actatcttaa tctggataca tgggagtgga 3241 atgaattaat tccacaaggc atatgcccag ttggtcgatc ttggcactca ctaacaccag 3301 tttcttcaga tcatcttttt ctctttggag gatttaccac tgataaacag ccactaagtg 3361 atgcctggac ttactgcatc agtaaaaatg aatggataca atttaatcat ccatataccg 3421 aaaaaccaag gttatggcac acagcttgtg ccagcgatga aggagaagta attgtttttg 3481 gtggatgtgc caacaacttg cttgtccatc acagagctgc acacagtaat gaaatactaa 3541 tattttcagt tcaaccaaaa tctcttgtac ggctaagctt agaagcagtc atttgcttta 3601 aagaaatgtt agccaactca tggaactgcc ttccaaaaca cttacttcac agtgttaatc 3661 agaggtttgg tagtaacaac acttctggat ctgtcgacGA CTACAAAGAC CATGACGGTG 3721 ATTATAAAGA TCATGACATC GACTACAAGG ATGACGATGA CAAGGGAAGC GGAGCCACTA 3781 ACTTCTCCCT GTTGAAACAA GCAGGGGATG TCGAAGAGAA TCCCGGGCCA gaattcGCCA 3841 CCatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg 3901 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct 3961 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca 4021 ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga 4081 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct 4141 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc 4201 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc 4261 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga 4321 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg 4381 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc 4441 actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg 4501 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt 4561 aaGGATCCaa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact 4621 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg 4681 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg 4741 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa 4801 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc 4861 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg 4921 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat 4981 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt 5041 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc 5101 cgcgtctccg ccttcgccct cagacgagtc ggatctccct ttggccgcct ccccgcctgg 5161 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa 5221 aggggggact ggaagggcta attcactccc aacgaaaata agatctgctt tttgcttgta 5281 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc 5341 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt 5401 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta 5461 gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat 5521 cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca 5581 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac 5641 tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc cgcccagttc 5701 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc 5761 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc tagacttttg 5821 cagagacggc ccaaattcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc 5881 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta 5941 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa 6001 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat 6061 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg 6121 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc 6181 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt 6241 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag 6301 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc 6361 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc 6421 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt 6481 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt 6541 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc 6601 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa 6661 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa 6721 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg 6781 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga 6841 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg 6901 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg 6961 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt 7021 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact 7081 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat 7141 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg 7201 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg 7261 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat 7321 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc 7381 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt 7441 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc 7501 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga 7561 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc 7621 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa 7681 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta 7741 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg 7801 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg 7861 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat 7921 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt 7981 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa 8041 aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct 8101 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag 8161 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc 8221 ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg 8281 cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga 8341 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc 8401 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc 8461 cagtgccaag ctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.