pMXs-Gata5-Puro plasmid

pMXs-Gata5-Puro plasmid

PVT101562

pMXs-Gata5-Puro plasmid Information

Plasmid name: pMXs-Gata5(mouse)-Puro
Alias: NM_008093.2;
Gene lenght: 1215bp
Host: Mammalian cells,Retrovirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter:
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: pol region-F1
Reverse primer:
Copy number: High
Induction:

pMXs-Gata5-Puro plasmid Description

pMXs-Gata5-Puro This is a plasmid suitable for protein expression in mammalian cells and can also be used for retrovirus packaging.

pMXs-Gata5-Puro plasmid Sequence

1 CCCGAAAAGT GCCACCTGCA TAATGAAAGA CCCCACCTGT AGGTTTGGCA AGCTAGCTTA 61 AGTAACGCCA TTTTGCAAGG CATGGAAAAA TACATAACTG AGAATAGAAA AGTTCAGATC 121 AAGGTCAGGA ACAGATGGAA CAGCTGAATA TGGGCCAAAC AGGATATCTG TGGTAAGCAG 181 TTCCTGCCCC GGCTCAGGGC CAAGAACAGA TGGAACAGCT GAATATGGGC CAAACAGGAT 241 ATCTGTGGTA AGCAGTTCCT GCCCCGGCTC AGGGCCAAGA ACAGATGGTC CCCAGATGCG 301 GTCCAGCCCT CAGCAGTTTC TAGAGAACCA TCAGATGTTT CCAGGGTGCC CCAAGGACCT 361 GAAATGACCC TGTGCCTTAT TTGAACTAAC CAATCAGTTC GCTTCTCGCT TCTGTTCGCG 421 CGCTTCTGCT CCCCGAGCTC AATAAAAGAG CCCACAACCC CTCACTCGGC GCGCCAGTCC 481 TCCGATTGAC TGAGTCGCCC GGGTACCCGT GTATCCAATA AACCCTCTTG CAGTTGCATC 541 CGACTTGTGG TCTCGCTGTT CCTTGGGAGG GTCTCCTCTG AGTGATTGAC TACCCGTCAG 601 CGGGGGTCTT TCATTTGGGG GCTCGTCCGG GATCGGGAGA CCCCTGCCCA GGGACCACCG 661 ACCCACCACC GGGAGGTAAG CTGGCCAGCA ACTTATCTGT GTCTGTCCGA TTGTCTAGTG 721 TCTATGACTG ATTTTATGCG CCTGCGTCGG TACTAGTTAG CTAACTAGCT CTGTATCTGG 781 CGGACCCGTG GTGGAACTGA CGAGTTCGGA ACACCCGGCC GCAACCCTGG GAGACGTCCC 841 AGGGACTTCG GGGGCCGTTT TTGTGGCCCG ACCTGAGTCC AAAAATCCCG ATCGTTTTGG 901 ACTCTTTGGT GCACCCCCCT AATAGGAGGG ATATGTGGTT CTGGTAGGAG ACGAGAACCT 961 AAAACAGTTC CCGCCTCCGT CTGAATTTTT GCTTTCGGTT TGGGACCGAA GCCGCGCCGC 1021 GCGTCTTGTC TGCTGCAGCA TCGTTCTGTG TTGTCTCTGT CTGACTGTGT TTCTGTATTT 1081 GTCTGAAAAT TAGGGCCAGA CTGTTACCAC TCCCTTAAGT TTGACCTTAG GTCACTGGAA 1141 AGATGTCGAG CGGATCGCTC ACAACCAGTC GGTAGATGTC AAGAAGAGAC GTTGGGTTAC 1201 CTTCTGCTCT GCAGAATGGC CAACCTTTAA CGTCGGATGG CCGCGAGACG GCACCTTTAA 1261 CCGAGACCTC ATCACCCAGG TTAAGATCAA GGTCTTTTCA CCTGGCCCGC ATGGACACCC 1321 AGACCAGGTC CCCTACATCG TGACCTGGGA AGCCTTGGCT TTTGACCCCC CTCCCTGGGT 1381 CAAGCCCTTT GTACACCCTA AGCCTCCGCC TCCTCTTCCT CCATCCGCCC CGTCTCTCCC 1441 CCTTGAACCT CCTCGTTCGA CCCCGCCTCG ATCCTCCCTT TATCCAGCCC TCACTCCTTC 1501 TCTAGGCGCC CCCATATGGC CATATGAGAT CTTATATGGG GCACCCCCGC CCCTTGTAAA 1561 CTTCCCTGAC CCTGACATGA CAAGAGTTAC TAACAGCCCC TCTCTCCAAG CTCACTTACA 1621 GGCTCTCTAC TTAGTCCAGC ACGAAGTCTG GAGACCTCTG GCGGCAGCCT ACCAAGAACA 1681 ACTGGACCGA CCGGTGGTAC CTCACCCTTA CCGAGTCGGC GACACAGTGT GGGTCCGCCG 1741 ACACCAGACT AAGAACCTAG AACCTCGCTG GAAAGGACCT TACACAGTCC TGCTGACCAC 1801 CCCCACCGCC CTCAAAGTAG ACGGCATCGC AGCTTGGATA CACGCCGCCC ACGTGAAGGC 1861 TGCCGACCCC GGGGGTGGAC CATCCTCTAG ACTGCCGGAT CTAGCTAGTT AATTAAGGAT 1921 CCgccaccat gtaccaaagc ttggcgttag cccagagccc cggccagggc acttacgccg 1981 actccggagc ctttctgcac tcttcgggaa ccggctcccc ggtgttcgtg gcacccactc 2041 gcatgccctc catgctgccg tacctgcctt cgtgtgagcc gggctcgcag gctcccgcgc 2101 tcgccgcgca ctccagctgg acacaagcag ttgccgctga ttcctcagcc ttcggctccg 2161 gaagcccgca cccgccagcc gcacacccac cgggagccac caccttcccg ttcgctcaca 2221 gccctccggg gtcgggcagc ggcggcagcg cgggggtccg ggacggcggc gccttccagg 2281 gcgcgctgct ggctcgcgaa cagtacccga ccccgctcgg gagacccatg ggcgcctcgt 2341 accccacaac ctacccagca tacatgagtt ccgacgtagc cccttcgtgg acctccggag 2401 ccttcgacag cagcatcctg cacggtctgc aagctcgccc tggtggcctc cctggccgca 2461 ggaccagctt cgtacctgac ttcttggagg agttccctgg tgagggccgg gagtgcgtca 2521 actgtggagc cctgtctaca ccactgtggc gcagggacgg caccggacac tatctatgca 2581 atgcctgcgg cctctatcac aagatgaacg gggtcaaccg accgctagtg aggccccaga 2641 agcgtctgtc ctcatcccga agatccggcc tatgctgctc caactgccat actgccacca 2701 ccaccctctg gaggcgcaac tcggagggcg aaccagtgtg caacgcctgt ggcctctaca 2761 tgaagctcca tggggtgcca aggccactgg caatgaaaaa ggagagtatc cagacaagaa 2821 aacggaagcc aaagaaccct gccaaaatca agggctcctc aggatccaca gcaaacacca 2881 cagcctcttc tcccactctc ctcaactctg agagctcagc caccactttg aaggcagagt 2941 ccagtctggc atctccagtg tgtgctgggc ccactatcac ctcccaggcc tccagcccag 3001 cggacgaatc cctggcctcc agccatttgg agttcaagtt cgaacctgaa gactttgcct 3061 tcacctcctc gtccatgagc ccccaggctg gtctcagtgg ggtcctacgt caggagacct 3121 ggtgtgctct ggccttggcc tagCTCGAGC GGCCGCCAGC ACAGTGGTCG ACAGCTGTGG 3181 AATGTGTGTC AGTTAGGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA 3241 AGCATGCATC TCAATTAGTC AGCAACCAGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC 3301 AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA TAGTCCCGCC CCTAACTCCG 3361 CCCATCCCGC CCCTAACTCC GCCCAGTTCC GCCCATTCTC CGCCCCATGG CTGACTAATT 3421 TTTTTTATTT ATGCAGAGGC CGAGGCCGCC TCGGCCTCTG AGCTATTCCA GAAGTAGTGA 3481 GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC AAAAAGCTTA CCATGACCGA GTACAAGCCC 3541 ACGGTGCGCC TCGCCACCCG CGACGACGTC CCCAGGGCCG TACGCACCCT CGCCGCCGCG 3601 TTCGCCGACT ACCCCGCCAC GCGCCACACC GTCGATCCGG ACCGCCACAT CGAGCGGGTC 3661 ACCGAGCTGC AAGAACTCTT CCTCACGCGC GTCGGGCTCG ACATCGGCAA GGTGTGGGTC 3721 GCGGACGACG GCGCCGCGGT GGCGGTCTGG ACCACGCCGG AGAGCGTCGA AGCGGGGGCG 3781 GTGTTCGCCG AGATCGGCCC GCGCATGGCC GAGTTGAGCG GTTCCCGGCT GGCCGCGCAG 3841 CAACAGATGG AAGGCCTCCT GGCGCCGCAC CGGCCCAAGG AGCCCGCGTG GTTCCTGGCC 3901 ACCGTCGGCG TCTCGCCCGA CCACCAGGGC AAGGGTCTGG GCAGCGCCGT CGTGCTCCCC 3961 GGAGTGGAGG CGGCCGAGCG CGCCGGGGTG CCCGCCTTCC TGGAGACCTC CGCGCCCCGC 4021 AACCTCCCCT TCTACGAGCG GCTCGGCTTC ACCGTCACCG CCGACGTCGA GTGCCCGAAG 4081 GACCGCGCGA CCTGGTGCAT GACCCGCAAG CCCGGTGCCT GACGCCCGCC CCACGACCCG 4141 CAGCGCCCGA CCGAAAGGAG CGCACGACCC ATCGTCGACG ATAAAATAAA AGATTTTATT 4201 TAGTCTCCAG AAAAAGGGGG GAATGAAAGA CCCCACCTGT AGGTTTGGCA AGCTAGCTTA 4261 AGTAACGCCA TTTTGCAAGG CATGGAAAAA TACATAACTG AGAATAGAGA AGTTCAGATC 4321 AAGGTCAGGA ACAGATGGAA CAGCTGAATA TGGGCCAAAC AGGATATCTG TGGTAAGCAG 4381 TTCCTGCCCC GGCTCAGGGC CAAGAACAGA TGGAACAGCT GAATATGGGC CAAACAGGAT 4441 ATCTGTGGTA AGCAGTTCCT GCCCCGGCTC AGGGCCAAGA ACAGATGGTC CCCAGATGCG 4501 GTCCAGCCCT CAGCAGTTTC TAGAGAACCA TCAGATGTTT CCAGGGTGCC CCAAGGACCT 4561 GAAATGACCC TGTGCCTTAT TTGAACTAAC CAATCAGTTC GCTTCTCGCT TCTGTTCGCG 4621 CGCTTCTGCT CCCCGAGCTC AATAAAAGAG CCCACAACCC CTCACTCGGG GCGCCAGTCC 4681 TCCGATTGAC TGAGTCGCCC GGGTACCCGT GTATCCAATA AACCCTCTTG CAGTTGCATC 4741 CGACTTGTGG TCTCGCTGTT CCTTGGGAGG GTCTCCTCTG AGTGATTGAC TACCCGTCAG 4801 CGGGGGTCTT TCACATGCAG CATGTATCAA AATTAATTTG GTTTTTTTTC TTAAGTATTT 4861 ACATTAAATG GCCATAGTTG CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC 4921 GTATTGGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC 4981 GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA 5041 ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG 5101 CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT 5161 CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA 5221 GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC 5281 TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT 5341 AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG 5401 CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG 5461 CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT 5521 TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC 5581 TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG 5641 CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC 5701 AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT 5761 AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTGCGGCCGG 5821 CCGCAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT 5881 CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC 5941 CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT 6001 ACCGCGAGAC CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG 6061 GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG 6121 CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC 6181 TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA 6241 ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG 6301 TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC 6361 ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA 6421 CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC 6481 AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG 6541 TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC 6601 CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC 6661 AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT 6721 ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG 6781 CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTC

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.