pLV3-CMV-MCS-3×FLAG-P2A-EGFP p

pLV3-CMV-MCS-3×FLAG-P2A-EGFP plasmid

PVT101531

pLV3-CMV-MCS-3×FLAG-P2A-EGFP plasmid Information

Plasmid name: pLV3-CMV-MCS-3×FLAG-P2A-EGFP
Alias: -
Gene lenght:
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Empty Vector
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-MCS-3×FLAG-P2A-EGFP plasmid Description

pLV3-CMV-MCS-3×FLAG-P2A-EGFP It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-MCS-3×FLAG-P2A-EGFP plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt 1921 atcgatacta gtcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga 1981 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt 2041 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt 2101 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca 2161 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt 2221 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt 2281 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca 2341 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg 2401 cggtaggcgt gtacggtggg aggtctatat aagcagagct ggtttagtga accgtcagat 2461 ccgctagcgc taccggactc agatctcgag ctcaagcttc GATATCCAgt cgacGACTAC 2521 AAAGACCATG ACGGTGATTA TAAAGATCAT GACATCGACT ACAAGGATGA CGATGACAAG 2581 GGAAGCGGAG CCACTAACTT CTCCCTGTTG AAACAAGCAG GGGATGTCGA AGAGAATCCC 2641 GGGCCAgaat tcGCCACCat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc 2701 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc 2761 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg 2821 cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc 2881 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc 2941 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag 3001 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac 3061 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg 3121 gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac 3181 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg 3241 ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag 3301 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg 3361 gacgagctgt acaagtaaGG ATCCaatcaa cctctggatt acaaaatttg tgaaagattg 3421 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct 3481 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg 3541 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact 3601 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc 3661 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc 3721 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag 3781 ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc 3841 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg 3901 gctctgcggc ctcttccgcg tctccgcctt cgccctcaga cgagtcggat ctccctttgg 3961 ccgcctcccc gcctggtacc tttaagacca atgacttaca aggcagctgt agatcttagc 4021 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aaaataagat 4081 ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct 4141 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta 4201 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca 4261 gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt 4321 gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac 4381 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt 4441 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc 4501 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg 4561 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg 4621 gaggcctaga cttttgcaga gacggcccaa attcgtaatc atggtcatag ctgtttcctg 4681 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta 4741 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg 4801 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga 4861 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg 4921 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag 4981 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc 5041 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca 5101 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt 5161 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc 5221 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc 5281 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc 5341 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact 5401 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg 5461 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta 5521 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca 5581 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa 5641 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg 5701 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc 5761 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg 5821 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat 5881 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg 5941 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa 6001 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca 6061 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc 6121 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt 6181 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa 6241 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat 6301 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct 6361 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga 6421 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag 6481 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga 6541 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca 6601 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg 6661 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc 6721 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag 6781 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca 6841 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg 6901 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag 6961 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg 7021 gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg 7081 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc 7141 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga 7201 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac 7261 gttgtaaaac gacggccagt gccaagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.